期刊
MACROMOLECULAR BIOSCIENCE
卷 16, 期 8, 页码 1158-1169出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/mabi.201600001
关键词
dental pulp stem cells; in vivo-forming hydrogel; osteogenic differentiation; tissue engineering
资金
- Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Education [2013R1A2A2A04014200, 2014M3A9E5073700]
- Priority Research Centers Program through the National Research Foundation of Korea (NRF) - Ministry of Education [2010-0028294]
- National Research Foundation of Korea [2013R1A2A2A04014200] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
In this study, human dental pulp stem cells (hDPSCs) are examined as a cellular source for bone tissue engineering using an in vivo-forming hydrogel. The hDPSCs are easily harvested in large quantities from extracted teeth. The stemness of harvested hDPSCs indicates their relative tolerance to ex vivo manipulation in culture. The in vitro osteogenic differentiation of hDPSCs is characterized using Alizarin Red S (ARS), von Kossa (VK), and alkaline phosphatase (ALP) staining. The solution of hDPSCs and a methoxy polyethylene glycol-polycaprolactone block copolymer (PC) is easily prepared by simple mixing at room temperature and in no more than 10 s it forms in vivo hydrogels after subcutaneous injection into rats. In vivo osteogenic differentiation of hDPSCs in the in vivo-forming hydrogel is confirmed by micro-computed tomography (CT), histological staining, and gene expression. Micro-CT analysis shows evidence of significant tissue-engineered bone formation in hDPSCs-loaded hydrogel in the presence of osteogenic factors. Differentiated osteoblasts in in vivo-forming hydrogel are identified by ARS and VK staining and are found to exhibit characteristic expression of genes like osteonectin, osteopontin, and osteocalcin. In conclusion, hDPSCs embedded in an in vivo-forming hydrogel may provide benefits as a noninvasive formulation for bone tissue engineering applications.
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