Article
Plant Sciences
Tsuyoshi Tanaka, Katsutomo Sasaki
Summary: This study aimed to investigate the axillary bud growth of chrysanthemum under temperature changes, and identified key genes for axillary bud development after cold treatment through transcriptome analysis. The results revealed that at least 101 genes in our dataset were homologous to transcription factors involved in axillary meristem development and growth in Arabidopsis, and six genes showed significant variations in expression under different conditions. The study suggests that these genes may be involved in the formation of axillary buds in chrysanthemum after cold treatment.
Article
Plant Sciences
Monica Canton, Cristian Forestan, Gianpiero Marconi, Esther Carrera, Claudio Bonghi, Serena Varotto
Summary: In temperate zones, fruit trees adjust their annual growth cycle to seasonal changes. Chilling accumulation allows peach flower bud development to be completed, accompanied by changes in transcription, epigenetic modifications, and chromatin structure.
Article
Horticulture
Wuwu Wen, Haimeng Fang, Lingqi Yue, Muhammad Khalil-Ur-Rehman, Yiqi Huang, Zhaoxuan Du, Guoshun Yang, Yanshuai Xu
Summary: The most common bud sport trait in grapevines is the change in color of grape berry skin, which is mainly regulated by the MYBA gene and the synthesis of anthocyanins. In this study, the expressions of genes related to grape berry bud sport skin color were evaluated using RNA-Seq technology, and it was found that the VvMYBA1/A2 gene was highly expressed in red grape varieties compared to white grape varieties at the veraison stage. Additionally, a specific gene (gene ID: Vitvi19g01871) showed the highest expressions in all samples, and its high expression level may play an important role in the bud sport color of grape berry skin at the veraison stage.
Article
Veterinary Sciences
Haoran Sun, Ke Meng, Yifan Wang, Yayan Wang, Xiaochun Yuan, Xinhai Li
Summary: Hair follicles in Dorper sheep exhibit seasonal cyclic growth and shedding. RNA sequencing analysis revealed potential regulatory mechanisms involved in the cyclic growth and development of hair follicles in Dorper sheep.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Huan Wang, Huabin Zhu, Zhihui Hu, Nuo Heng, Jianfei Gong, Yi Wang, Huiying Zou, Shanjiang Zhao
Summary: The Pc mutation locus is a simple single mutation for breeding polled cattle. The mechanism of Pc locus involves inhibition of neural crest cell EMT generation and keratin expression, affecting the migration and keratinization of the horn bud tissue.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Developmental Biology
Hussein A. Saud, Paul A. O'Neill, Yosuke Ono, Bas Verbruggen, Ronny Van Aerle, Jaebum Kim, Jae-Seong Lee, Brian C. Ring, Tetsuhiro Kudoh
Summary: This study characterized two mutants in self-fertilizing mangrove killifish, with identified mutated genes for rapid identification. The findings showcase the potential for accelerating future small-scale forward-genetic screening and mutation identification.
Article
Biotechnology & Applied Microbiology
Junmin He, Bingru Zhao, Xixia Huang, Xuefeng Fu, Guifen Liu, Yuezhen Tian, Cuiling Wu, Jingyi Mao, Jing Liu, Shuangbao Gun, Kechuan Tian
Summary: This study identified differentially expressed genes during the development of hair follicles in Merino sheep and identified key genes affecting hair follicle morphogenesis through network analysis. These findings are important for understanding fleece evolution in animals and provide a theoretical basis for breeding fine wool sheep.
Article
Multidisciplinary Sciences
Racheli Wercberger, Joao M. Braz, Jarret A. Weinrich, Allan I. Basbaum
Summary: Using retro-TRAP and RNA sequencing, researchers have uncovered extensive molecular diversity of spino-and trigeminoparabrachial projection neurons, including distinct subsets of gene expression. Further investigation revealed significant functional heterogeneity in these projection neurons, showing convergence and segregation of pain-and itch-provoking inputs into molecularly diverse subsets of NK1R- and non-NK1R-expressing neurons.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Agriculture, Dairy & Animal Science
Daqing Wang, Yanyan Yang, Yongli Song, Shaoyin Fu, Xiaolong He, Biao Wang, Liwei Wang, Xin Chen, Xihe Li, Yongbin Liu, Guifang Cao
Summary: The addition of ghrelin to the in vitro maturation system of sheep oocytes was found to shorten the maturation time and hinder early embryonic development. The RNA-seq analysis revealed alterations in cell cycle signaling pathways and the inhibition of P53. Overall, these findings provide new insights into the effects of exogenous ghrelin on sheep oocyte maturation and early embryonic development in vitro.
Article
Genetics & Heredity
Zhanyue Wu, Meng Liu, Mingyi Yan, Shutong Dong, Sen Wu
Summary: This study aims to accurately identify the core regulatory genes in the process of muscle development of black Tibetan sheep, further explore the physiological processes of growth, development, and myogenesis of black Tibetan sheep, and carry out molecular breeding of black Tibetan sheep. The expression of genes during muscle development at different stages was quantified, and overexpression and interference techniques were used to detect the role of core genes in the proliferation of primary muscle cells of black Tibetan sheep. The importance of core genes in different developmental stages and their potential interaction mechanisms were investigated. Overall, the research is rated as 8 out of 10 in terms of importance.
Article
Food Science & Technology
Shishuai Sun, Chunyu Cao, Jibo Li, Qingwei Meng, Baojing Cheng, Baoming Shi, Anshan Shan
Summary: Lycopene plays a vital role in improving immunity, enhancing antioxidant capacity, and regulating fat metabolism. The study found that under a high-fat diet, lycopene can reduce oxidative stress, inflammation, and fat deposition in the placenta, promoting fetal development. Lycopene supplementation decreases levels of IL-17, IL-6, and TNF-alpha, increases expression of Glrx gene and protein, and enhances antioxidant capacity in the placenta.
MOLECULAR NUTRITION & FOOD RESEARCH
(2021)
Article
Genetics & Heredity
Yuchen Li, Weihong Zeng, Tong Li, Yanyan Guo, Guangyong Zheng, Xiaoying He, Lilian Bai, Guolian Ding, Li Jin, Xinmei Liu
Summary: The study conducted integrative single-cell analyses of thymocytes at different gestational ages, identifying candidate genes regulating T cell receptor lineage selection and characterizing the trajectory of early thymocyte commitment during fetal growth. Comparisons with mouse data revealed both conserved and species-specific transcriptional dynamics, as well as susceptibility genes associated with autoimmune disorders.
FRONTIERS IN GENETICS
(2021)
Article
Agriculture, Dairy & Animal Science
Taotao Li, Meilin Jin, Xiaojuan Fei, Zehu Yuan, Yuqin Wang, Kai Quan, Tingpu Wang, Junxiang Yang, Maochang He, Caihong Wei
Summary: This study investigates the difference in liver fat metabolism between two sheep breeds, Hu sheep and Tibetan sheep, using RNA-seq technology. Differentially expressed genes and enriched pathways related to fat metabolism were identified, and the expression levels of several genes were validated at both the mRNA and protein levels. The results reveal the difference in fat metabolism between the two sheep breeds, suggesting that Tibetan sheep have a higher level of fatty acid oxidation in the liver compared to Hu sheep. The enrichment of fat-metabolism-related genes in the thermogenesis pathway indicates their impact on adaptive thermogenesis in Tibetan sheep.
Article
Cell Biology
Aleksandra Lipka, Jan Pawel Jastrzebski, Lukasz Paukszto, Karol Gustaw Makowczenko, Elzbieta Lopienska-Biernat, Marek Gowkielewicz, Ewa Lepiarczyk, Marta Wiszpolska, Mariusz Krzysztof Majewski, Marta Majewska
Summary: Impaired fetal growth is a major cause of prematurity, stillbirth, and infant mortality. This study investigated non-coding RNAs in the placentas of male and female fetuses with FGR. Dysregulation of lncRNAs was found to affect vasculature in female placentas, while immune processes were more affected in male placentas.
Editorial Material
Biology
Peter Kalds, Shuhong Huang, Yulin Chen, Xiaolong Wang
Summary: Understanding the genetic basis of sheep tails is crucial for improving sheep production and welfare. Recent findings reveal that the HOXB13 gene regulates tail length in sheep, providing new insights into the genetic determination of this phenotype.
COMMUNICATIONS BIOLOGY
(2022)
Article
Agronomy
Tianyou Bai, Benmeng Liang, Yuhetian Zhao, Jiangang Han, Yabin Pu, Chunxin Wang, Yuehui Ma, Lin Jiang
Summary: This study conducted a transcriptomic analysis of the skin of different breeds of sheep at different growth stages to explore the molecular mechanisms of skin and hair diversity regulation. The results identified differentially expressed genes and transcription factors associated with hair follicle development. Additionally, key pathways and candidate genes involved in skin structure differences were identified. This study provides insights into the genetic structure and gene regulation in the skin and hair growth process.
Article
Agronomy
Ruonan Li, Yuehui Ma, Lin Jiang
Summary: The dairy sheep industry is a significant but underdeveloped part of the small ruminant industry. Sheep milk has higher nutritional value compared to goat milk and cow milk, with abundant fat yield, protein percentage, and mineral contents. There have been studies on genetic factors influencing milk production and composition traits, providing opportunities for the development of the sheep milk industry. However, further research is still needed in this field.
Article
Agriculture, Dairy & Animal Science
Ling Li, Yefang Li, Qing Ma, Shuqin Liu, Yuehui Ma, Lin Jiang
Summary: This study investigated the family structure, parent-child pair, and inbreeding coefficient of a Tan sheep breeding farm in Ningxia, China. The results provide the theoretical basis for selecting excellent breeding stock and making an appropriate mating plan, as well as the scientific basis for the conservation and reproduction of Tan sheep germplasm resources.
Article
Agronomy
Benmeng Liang, Yuhetian Zhao, Yabin Pu, Xiaohong He, Jiangang Han, Baima Danzeng, Yuehui Ma, Jianfeng Liu, Lin Jiang
Summary: Through the analysis of 448 individuals from 24 Tibetan sheep populations in different regions, we successfully constructed specific SNP genetic marker sets for the identification of Tibetan sheep breeds at the molecular level, including Yunnan-Tibetan sheep, Zuogong, Heizang, Gongga, and Tao sheep.
Article
Agronomy
Dandan Wang, Shengnan Wang, Wenjie Tian, Yuehui Ma, Lin Jiang
Summary: This study found that ZBED6 KO pigs had heavier spleens. RNA sequencing analysis revealed significantly upregulated IGF2 mRNA in the spleen of ZBED6 KO pigs. Functional enrichment analysis showed enrichment mainly in myofibril assembly and sarcomere. IGF2, DE-lnRNAs, and ACTN2 may be the key candidate genes in promoting spleen growth in ZBED6 KO pigs.
Article
Agriculture, Dairy & Animal Science
Qian Chen, Chang Huang, Yinxiao Su, Qian Zhao, Yabin Pu, Xiaohong He, Lin Jiang, Yuehui Ma, Qianjun Zhao, Shaohui Ye
Summary: In this study, the transcriptional profile of ovine skeletal muscle satellite cells was constructed using the RNA-Seq method. A total of 1954 DEGs, 1479 AS, and 253 TFs were detected during SMSC proliferation and differentiation. GO and KEGG analyses showed enrichment of the MAPK signaling pathway, PI3K-Akt signaling pathway, Wnt signaling pathway, and Ras signaling pathway. This study provides valuable insights into the transcriptional regulation of SMSCs during proliferation and differentiation and contributes to our understanding of the molecular mechanisms underlying myogenesis and muscle development.
Article
Agriculture, Dairy & Animal Science
Ruonan Li, Yuhetian Zhao, Benmeng Liang, Yabin Pu, Lin Jiang, Yuehui Ma
Summary: In this study, whole-genome sequences were analyzed to investigate the genetic signatures of milk production in dairy sheep, and 553 genes were found to be related to milk production. The validation experiment showed that the FCGR3A gene has a significant negative relationship with milk production. This study provides a basis for further research on the genetic mechanism underlying milk production traits in sheep.
Article
Biology
Ling Liu, Shengnan Wang, Wenjie Tian, Cheng Xu, Chengjie Wei, Kai Cui, Lin Jiang, Dandan Wang
Summary: Zbed6 single-allele knockout promotes an increase in skeletal muscle weight and muscle fiber area in mice by regulating another target gene.