期刊
出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.2105732118
关键词
pain; itch; projection neurons; dorsal horn; RNA-seq
资金
- NIH [NSR35097306]
- Open Philanthropy
- NSF Graduate Research Program
Using retro-TRAP and RNA sequencing, researchers have uncovered extensive molecular diversity of spino-and trigeminoparabrachial projection neurons, including distinct subsets of gene expression. Further investigation revealed significant functional heterogeneity in these projection neurons, showing convergence and segregation of pain-and itch-provoking inputs into molecularly diverse subsets of NK1R- and non-NK1R-expressing neurons.
A remarkable molecular and functional heterogeneity of the primary sensory neurons and dorsal horn interneurons transmits pain-and or itch-relevant information, but the molecular signature of the projection neurons that convey the messages to the brain is unclear. Here, using retro-TRAP (translating ribosome affinity purification) and RNA sequencing, we reveal extensive molecular diversity of spino-and trigeminoparabrachial projection neurons. Among the many genes identified, we highlight distinct subsets of Cck(+)-, Nptx2(+)-, Nmb(+)-, and Crh(+)-expressing projection neurons. By combining in situ hybridization of retrogradely labeled neurons with Fos-based assays, we also demonstrate significant functional heterogeneity, including both convergence and segregation of pain-and itch-provoking inputs into molecularly diverse subsets of NK1R- and non-NK1R- expressing projection neurons.
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