Article
Medicine, Research & Experimental
Yulin Wang, Fabian De Labastida Rivera, Chelsea L. Edwards, Teija C. M. Frame, Jessica A. Engel, Luzia Bukali, Jinrui Na, Susanna S. Ng, Dillon Corvino, Marcela Montes de Oca, Patrick T. Bunn, Megan S. F. Soon, Dean Andrew, Jessica R. Loughland, Jia Zhang, Fiona H. Amante, Bridget E. Barber, James S. Mccarthy, J. Alejandro Lopez, Michelle J. Boyle, Christian R. Engwerda
Summary: The development of highly effective malaria vaccines and improvement of drug-treatment protocols are important for malaria elimination. However, the establishment of parasite-specific immune regulatory networks hinder these efforts. This study identified STING as a critical mediator of type I interferon production by CD4+ T cells during malaria infection, leading to the development of specific CD4+ T cells.
JOURNAL OF CLINICAL INVESTIGATION
(2023)
Article
Virology
Nozomi Kuse, Tomohiro Akahoshi, Masafumi Takiguchi
Summary: The study demonstrated that priming with STING ligand 3'3'-cGAMP effectively activated HLA-B*52:01-restricted CD8(+) T cells, which showed strong ability to suppress HIV-1 replication and expressed high levels of cytolytic effector molecules. This suggests that priming with STING ligand of functional CD8(+) T cells specific for protective epitopes could be beneficial in an HIV-1 cure therapy.
JOURNAL OF VIROLOGY
(2021)
Article
Oncology
Anlin Li, Yuanyuan Wang, Zhixin Yu, Zihui Tan, Lina He, Sha Fu, Mengting Shi, Wei Du, Linfeng Luo, Zhichao Li, Jiaqing Liu, Yixin Zhou, Wenfeng Fang, Yunpeng Yang, Li Zhang, Shaodong Hong
Summary: In non-small cell lung cancer, STK11 phenotype rather than mutation status accurately identifies patients with immunotherapy-refractory disease and reflects immune suppression.
JOURNAL OF THORACIC ONCOLOGY
(2023)
Article
Multidisciplinary Sciences
Takayuki Chikata, Hiroyuki Gatanaga, Hung The Nguyen, Daisuke Mizushima, Yu Zhang, Nozomi Kuse, Shinichi Oka, Masafumi Takiguchi
Summary: This study investigated HIV-1-specific CD8(+) T cell responses in 200 Japanese HIV-1-exposed seronegative (HESN) men who have sex with men (MSM). The results showed the presence of HLA-B*51:01-restricted Pol TI8- specific and HLA-A*02:06-restricted Pol SV9-specific CD8+ T cells in two and one individuals, respectively. These HIV-1-specific T cells may contribute to suppressing HIV-1 replication in HESN-MSM individuals.
Article
Immunology
Raquel de Souza Vieira, Marilda Savoia Nascimento, Isau Henrique Noronha, Jose Ronnie Carvalho Vasconcelos, Luiz Alberto Benvenuti, Glen N. Barber, Niels Olsen Saraiva Camara, Jorge Kalil, Edecio Cunha-Neto, Rafael Ribeiro Almeida
Summary: STING signaling pathway plays an important role in immune response to Trypanosoma cruzi infection, and its absence negatively affects the activation of IRF-dependent pathways and cytokine expression.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
An-Liang Guo, Jin-Fang Zhao, Lin Gao, Hui-Huang Huang, Ji-Yuan Zhang, Chao Zhang, Jin-Wen Song, Ruo-Nan Xu, Xing Fan, Ming Shi, Yan-Mei Jiao, Fu-Sheng Wang
Summary: The study found that CD11c(+) CD8(+) T cells can effectively control viral replication during HIV-1 infection and have the potential to be used as an immunotherapeutic approach.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Konjit Getachew Muleta, Isabel Ulmert, Kedir Hussen Hamza, Sharne van Dijl, Joy Nakawesi, Katharina Lahl
Summary: Rotavirus (RV) infection induces strong adaptive immunity, with initial clearance depending on cytotoxic CD8 T cells. Type I classical dendritic cells (cDC1) play a key role in CD8 T cell induction and RV cytotoxicity. This study found that the individual Toll-like receptor 3 (TLR3) and MyD88-mediated signaling pathways were not required for CD8 T cell response or RV clearance. Surprisingly, absence of type I interferon (IFN) signaling did not alter the accumulation of RV-specific CD8 T cells, but it impaired their ability to produce IFN gamma and granzyme.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Virology
Jian Deng, Sheng-Nan Zheng, Yang Xiao, Mei-Ling Nan, Jing Zhang, Lulu Han, Yi Zheng, Yanying Yu, Qiang Ding, Chengjiang Gao, Pei-Hui Wang
Summary: SARS-CoV-2 NSP8 inhibits the production of type I and III interferons by targeting RIG-I/MDA5, TRIF, and STING signaling molecules. It disrupts the assembly of RIG-I/MDA5-MAVS complex, resulting in impaired phosphorylation and nuclear translocation of IRF3. NSP8 also directly interacts with TRIF and STING to suppress their signaling transduction.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Review
Cell Biology
Nadia Duarte, Abdul Muktadir Shafi, Carlos Penha-Goncalves, Teresa Faria Pais
Summary: The endothelium layer lining the inner surface of blood vessels plays important physiological roles and participates in immune responses. Induction and signaling of type I interferon in endothelial cells at the blood-brain barrier have been extensively studied. Abnormal endothelial cell function and brain inflammation are associated with excessive STING activation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Immunology
Leila Khajavi, Xuan-Hung Nguyen, Clemence Queriault, Marianne Chabod, Lucie Barateau, Yves Dauvilliers, Matthias Zytnicki, Roland Liblau
Summary: This study identified differentially expressed genes in T-cell subsets between narcolepsy patients and healthy controls through RNA sequencing analysis, and found that these genes are involved in processes such as immune synapse formation, TCR activation, and inflammatory response. The results showed an inflammatory transcriptomic profile in naive CD4 and CD8 T-cell subsets, possibly indicating their involvement in the development or progression of narcolepsy.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Virology
Jian Deng, Yi Zheng, Sheng-Nan Zheng, Mei-Ling Nan, Lulu Han, Jing Zhang, Yunyun Jin, Ji-An Pan, Chengjiang Gao, Pei-Hui Wang
Summary: The NSP7 protein of SARS-CoV-2 inhibits the production of interferons, crucial components of antiviral immunity, by targeting multiple signaling pathways. It disrupts the normal functioning of RIG-I/MDA5, TLR3-TRIF, and cGAS-STING pathways, leading to a dampened interferon response. Additionally, NSP7 decreases immune activation and facilitates virus replication.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Immunology
Avishay Dolitzky, Inbal Hazut, Shmulik Avlas, Sharon Grisaru-Tal, Michal Itan, Ilan Zaffran, Francesca Levi-Schaffer, Motti Gerlic, Ariel Munitz
Summary: This study demonstrates that eosinophils can interact with apoptotic cells, leading to polarized activation towards an anti-inflammatory phenotype. Apoptotic cells suppress the inflammatory responses of eosinophils and enhance the activation induced by specific cytokines. These findings reveal the heterogeneity of eosinophil activation patterns and highlight the potential role of apoptotic cells in regulating eosinophil polarization.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Leeana D. Peters, Wen- Yeh, Juan M. Arnoletti, Matthew E. Brown, Amanda L. Posgai, Clayton E. Mathews, Todd M. Brusko
Summary: The autoimmune pathogenesis of T1D involves immune cell infiltration into pancreatic islets. CD8(+) T cells play a primary role in killing insulin-producing beta-cells. This study demonstrates the engineering of human T cells to improve the specificity and function of autoreactive CD8(+) T cells, potentially leading to cellular therapeutics for treating autoimmunity.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Cell Biology
Laura L. Dickey, Laura J. Martins, Vicente Planelles, Timothy M. Hanley
Summary: Macrophages chronically infected with HIV-1 can enter a latent state resembling latency, characterized by decreased viral transcription and maintenance of a non-permissive state. This transition to viral latency is mediated by type I interferon signaling and requires the viral accessory protein Vpr. Understanding the mechanisms of HIV-1 latency in macrophages can lead to potential therapeutic targets for eliminating this important HIV-1 reservoir.
JOURNAL OF LEUKOCYTE BIOLOGY
(2022)
Article
Cell Biology
Hannah L. Itell, Daryl Humes, Julie Overbaugh
Summary: This study identifies new ISGs that inhibit HIV and confirms the role of certain factors in IFN restriction in T cells. The findings demonstrate the multifaceted nature of IFN restriction against HIV and establish a screening model for identifying HIV-restricting ISGs.
Article
Immunology
Pierre Gantner, Supranee Buranapraditkun, Amelie Pagliuzza, Caroline Dufour, Marion Pardons, Julie L. Mitchell, Eugene Kroon, Carlo Sacdalan, Nicha Tulmethakaan, Suteeraporn Pinyakorn, Merlin L. Robb, Nittaya Phanuphak, Jintanat Ananworanich, Denise Hsu, Sandhya Vasan, Lydie Trautmann, Remi Fromentin, Nicolas Chomont
Summary: During early stages of HIV infection, the virus initially targets a small population of proliferating memory CD4+ T cells with high surface expression of CCR5. Productively infected cells exhibit different phenotypes and TCR sequences depending on the stage of infection and location within the body. The TCR repertoire of infected cells is biased towards previously expanded and disseminated clones, suggesting independent infection events. Latent genetically intact proviruses are present early in infection, indicating simultaneous generation of latent infected cells.
Article
Biochemistry & Molecular Biology
Sara Bolivar-Marin, Marcela Castro, Diego Losada-Floriano, Santiago Cortes, Federico Perdomo-Celis, Giovani Lastra, Carlos F. Narvaez
Summary: Increased levels of inflammatory cytokines, such as IL-6, IL-10, and sTNFRI, are associated with respiratory failure, immune dysregulation, coagulopathy, and mortality in individuals with COVID-19.
JOURNAL OF INTERFERON AND CYTOKINE RESEARCH
(2023)
Article
Immunology
Chris Y. Y. Chiu, Maya D. D. Schou, James H. H. McMahon, Steven G. G. Deeks, Remi Fromentin, Nicolas Chomont, Michelle N. N. Wykes, Thomas A. A. Rasmussen, Sharon R. R. Lewin
Summary: The expression of immune checkpoint proteins is elevated in people with HIV, both on and off antiretroviral therapy (ART). Soluble immune checkpoint proteins and their ligands can be detected in plasma and may correlate with HIV reservoir size and T-cell function. Further investigation is needed to understand the role of these soluble proteins in the treatment and cure of HIV infection.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Microbiology
Hernando Gutierrez-Barbosa, Sandra Medina-Moreno, Federico Perdomo-Celis, Harry Davis, Carolina Coronel-Ruiz, Juan C. Zapata, Joel V. Chua
Summary: Humanized mice are valuable for studying human diseases, but it is important to understand their strengths and limitations and choose the appropriate model. This study found that the Hu-SGM3 model consistently produced higher numbers of various human immune cells, while the hu-NOG-EXL model had a higher number of circulating platelets in an inactivated state. The hu-NSG and hu-NCG models had low frequencies of immune cells. Therefore, selecting the right humanized mouse model is crucial for specific research questions.
Review
Multidisciplinary Sciences
Valentina Restrepo Arenas, Maria T. Rugeles, Federico Perdomo-Celis, Natalia Taborda
Summary: Achieving a cure for HIV infection is a global priority, and CD8+ T cells play a central role in the natural control of HIV, suggesting their potential for use in achieving remission or cure. This work reviews the challenges, models, and mechanisms related to HIV control mediated by CD8+ T cells, and discusses strategies based on this cell population in the search for an HIV cure. Finally, the current challenges and perspectives in translating this knowledge into scalable HIV cure strategies are analyzed.
Article
Microbiology
Mathieu Dube, Olivier Tastet, Caroline Dufour, Geremy Sannier, Nathalie Brassard, Gloria-Gabrielle Delgado, Amelie Pagliuzza, Corentin Richard, Manon Nayrac, Jean-Pierre Routy, Alexandre Prat, Jacob D. Estes, Remi Fromentin, Nicolas Chomont, Daniel E. Kaufmann
Summary: During suppressive antiretroviral therapy, spontaneous transcription and translation of HIV can persist, with p24(-)expressing cells evident in 39% of participants. Active reservoirs are enriched in central memory T cells and CCR6(-) and activation marker-expressing cells. The magnitude of the active reservoir positively correlates with HIV-specific CD4(+) and CD8(+) T cell responses and multiple HIV-specific T cell clusters.
CELL HOST & MICROBE
(2023)
Article
Microbiology
Hiroshi Takata, Julie L. Mitchell, Julian Pacheco, Amelie Pagliuzza, Suteeraporn Pinyakorn, Supranee Buranapraditkun, Carlo Sacdalan, Louise Leyre, Sam Nathanson, Juyeon C. Kakazu, Jintana Intasan, Peeriya Prueksakaew, Nitiya Chomchey, Nittaya Phanuphak, Mark de Souza, Elias K. Haddad, Morgane Rolland, Sodsai Tovanabutra, Sandhya Vasan, Denise C. Hsu, Nicolas Chomont, Lydie Trautmann
Summary: This study found that HIV-specific CD8+ T cell responses and HIV reservoir declined significantly after 2 years of antiretroviral therapy (ART). Sustained HIV-specific CD8+ T cell responses were associated with a greater reduction of integrated HIV provirus. However, the magnitude of CD8+ T cells specific for different HIV proteins positively correlated with the active reservoir size during ART. High HIV DNA levels were strongly associated with maintenance of short-lived HIV-specific CD8+ T cells regardless of the time of ART initiation.
CELL HOST & MICROBE
(2023)
Article
Immunology
Doris M. Salgado, Gina M. Rivera, William A. Pinto, Jairo Rodriguez, Gladys Acosta, Diana M. Castaneda, Rocio Vega, Federico Perdomo-Celis, Irene Bosch, Carlos F. Narvaez
Summary: This study investigates the differential pattern and dynamic of soluble factors between pediatric dengue and sepsis, and their relationship with clinical severity. The results suggest that cytokine responses play an important role in the pathophysiology and clinical outcome of both diseases, offering potential new diagnosis and therapeutic strategies.
PEDIATRIC INFECTIOUS DISEASE JOURNAL
(2023)
Article
Cell Biology
Caroline Dufour, Maria Julia Ruiz, Amelie Pagliuzza, Corentin Richard, Aniqa Shahid, Remi Fromentin, Rosalie Ponte, Amelie Cattin, Tomas Raul Wiche Salinas, Syim Salahuddin, Teslin Sandstrom, Stephanie Burke Schinkel, Cecilia T. Costiniuk, Mohammad-Ali Jenabian, Petronela Ancuta, Jean-Pierre Routy, Eric A. Cohen, Zabrina L. Brumme, Christopher Power, Jonathan B. Angel, Nicolas Chomont
Summary: HIV persists in various tissues during antiretroviral therapy, with different anatomical compartments contributing differently to the viral reservoir. In two individuals on suppressive ART, HIV DNA was found in all tissues, with significant variations across compartments. Intact HIV genomes were mainly detected in secondary lymphoid organs, indicating the potential migration and circulation of infected cells between anatomical sites.
Article
Cell Biology
Ralph-Sydney Mboumba Bouassa, Eve Comeau, Yulia Alexandrova, Amelie Pagliuzza, Alexis Yero, Suzanne Samarani, Judy Needham, Joel Singer, Terry Lee, Florian Bobeuf, Claude Vertzagias, Giada Sebastiani, Shari Margolese, Enrico Mandarino, Marina B. Klein, Bertrand Lebouche, Jean-Pierre Routy, Nicolas Chomont, Cecilia T. Costiniuk, Mohammad-Ali Jenabian
Summary: Chronic HIV infection is accompanied by persistent inflammation, and this study suggests that cannabinoids may help reduce systemic inflammation in people with HIV. The study found that oral cannabinoids, either in combination with THC and CBD or CBD-only, significantly reduced inflammatory markers and affected immune cell subsets. These findings can guide future large clinical trials investigating the anti-inflammatory properties of cannabinoids.
Article
Medicine, General & Internal
Lena Royston, Stephane Isnard, Carolina A. Berini, Simeng Bu, Peter L. Lakatos, Talat Bessissow, Nicolas Chomont, Marina Klein, Bertrand Lebouche, Alexandra de Pokomandy, Nadine Kronfli, Cecilia T. Costiniuk, Rejean Thomas, Cecile Tremblay, Guy Boivin, Jean-Pierre Routy
Summary: This study aims to assess whether letermovir, a novel anti-CMV drug, can inhibit CMV subclinical replication in HIV patients receiving ART, and subsequently reduce CMV-associated gut damage and inflammation. The study will conduct a 14-week open-label, randomized, controlled clinical trial with 60 CMV-seropositive ART-treated HIV patients. The effects of letermovir on gut damage, microbial translocation, inflammation, and HIV reservoir size will be evaluated.
Article
Immunology
Mehdi Benlarbi, Jonathan Richard, Catherine Bourassa, William D. Tolbert, Carl Chartrand-Lefebvre, Gabrielle Gendron-Lepage, Mohamed Sylla, Mohamed El-Far, Marc Messier-Peet, Camille Guertin, Isabelle Turcotte, Remi Fromentin, Myriam Maude Verly, Jeremie Prevost, Andrew Clark, Walther Mothes, Daniel E. Kaufmann, Frank Maldarelli, Nicolas Chomont, Philippe Begin, Cecile Tremblay, Jean-Guy Baril, Benoit Trottier, Sylvie Trottier, Ralf Duerr, Marzena Pazgier, Madeleine Durand, Andres Finzi
Summary: This study found that soluble glycoprotein 120 (sgp120) and gp120-specific anti-cluster A antibodies are associated with immune dysfunction, chronic inflammation, and subclinical cardiovascular disease in HIV-infected individuals.
JOURNAL OF INFECTIOUS DISEASES
(2023)