Article
Cell Biology
Elena Shersher, Mohini Lahiry, Annamil Alvarez-Trotta, Giulia Diluvio, David J. Robbins, Ramin Shiekhattar, Anthony J. Capobianco
Summary: This study identifies the INT complex as a novel co-factor in Notch-mediated transcription that together with NACK activates Notch target genes and leads to cancer cell proliferation.
CELL COMMUNICATION AND SIGNALING
(2021)
Article
Pharmacology & Pharmacy
Shuzo Urata, Olaposi Idowu Omotuyi, Ayako Izumisawa, Takeshi Ishikawa, Satoshi Mizuta, Yasuteru Sakurai, Tatsuaki Mizutani, Hiroshi Ueda, Yoshimasa Tanaka, Jiro Yasuda
Summary: This study reveals the dimerization mechanism of VP40 and identifies two compounds through computational screening and in vitro assays, which may serve as lead drug candidates against Filovirus diseases.
ANTIVIRAL RESEARCH
(2022)
Article
Cell Biology
Ramesh Yelagandula, Karin Stecher, Maria Novatchkova, Luca Michetti, Georg Michlits, Jingkui Wang, Pablo Hofbauer, Gintautas Vainorius, Carina Pribitzer, Luke Isbel, Sasha Mendjan, Dirk Schuebeler, Ulrich Elling, Julius Brennecke, Oliver Bell
Summary: ZNF462 haploinsufficiency is associated with Weiss-Kruszka syndrome, which is characterized by neurodevelopmental defects including autism. However, the molecular functions of ZNF462 have remained unclear. This study identifies ZFP462 as the murine homologue of ZNF462 and demonstrates its role in regulating neural lineage specification in mouse embryonic stem cells (ESCs) by interacting with the H3K9-specific histone methyltransferase complex G9A/GLP to silence meso-endodermal genes.
NATURE CELL BIOLOGY
(2023)
Article
Cell Biology
Silvia T. Erni, John C. Gill, Carlotta Palaferri, Gabriella Fernandes, Michelle Buri, Katherine Lazarides, Denis Grandgirard, Albert S. B. Edge, Stephen L. Leib, Marta Roccio
Summary: The study evaluated the otoregenerative potential of different gamma-secretase inhibitors (GSIs) in various assay models and found that GSIs induced hair cell gene expression and increased hair cell numbers, especially outer hair cells, in different models. These findings suggest the potential of targeting Notch signaling with novel GSIs for hearing restoration, paving the way for more complex animal studies and clinical research.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Fangquan Chen, Shan Zhu, Rui Kang, Daolin Tang, Jiao Liu
Summary: In this study, the researchers identified ATP6V0D1 as a direct target of JTC801, which drives alkaliptosis in human PDAC cells. The interaction between ATP6V0D1 and STAT3 increases STAT3 expression and activity, maintaining lysosome homeostasis. Inhibition of STAT3 restores the sensitivity of ATP6V0D1-deficient cells to alkaliptosis. Furthermore, high expression of ATP6V0D1 correlates with prolonged survival in PDAC patients.
Article
Oncology
Yanqiong Chen, Wenting Zhang, Xiufeng Bai, Yi Liu
Summary: This study developed a new method to specifically inhibit the activity of the transcription factor STAT3, which successfully blocked its transcription activity in cancer cells and provided a novel approach for the treatment of cancer and autoimmune diseases.
Review
Biochemistry & Molecular Biology
Wantae Kim, Blase LeBlanc, Wendy L. Matthews, Zhong-Yin Zhang, Yan Zhang
Summary: Phosphorylation of RNA polymerase II is crucial for the temporal progression of eukaryotic transcription, and chemical genetic methods have been used to investigate pathways regulated by kinases and phosphatases targeting RNAP II. Recent advancements in chemical biology have led to the discovery of inhibitors and activators for kinases/phosphatases, providing new avenues for studying RNA polymerase II-mediated transcription.
CURRENT OPINION IN CHEMICAL BIOLOGY
(2021)
Article
Immunology
Qiuyu Wu, Xiangjie Chen, Caixia Qiao, Xinhua Cao, Qian Du, Yukang Yuan, Yibo Zuo, Ying Miao, Zhijin Zheng, Tingting Zhang, Lichao Zang, Xinyu Yang, Weifeng Shi, Zhijun Xie, Yang Xu, Depei Wu, Chengping Wen, Hui Zheng
Summary: Methotrexate (MTX) is commonly used to treat rheumatoid arthritis, acute leukemia, and psoriasis. However, the exact mechanism of myelosuppression caused by MTX is still unknown. This study explores the impact of MTX on the Notch signaling pathway and finds that MTX strongly suppresses this pathway by stabilizing Numb proteins and reducing Notch1 protein levels. Interestingly, the monomeric drug Triptolide can alleviate the inhibitory effect of MTX on the Notch signaling pathway.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Oncology
Bing Chen, Ya-Nan Deng, Xixi Wang, Zijing Xia, Yu He, Peng Zhang, Samina Ejaz Syed, Qiu Li, Shufang Liang
Summary: miR-26a is a key regulator of CRC tumorigenesis that enhances AKT1 transcription by improving the deacetylation modification of RREB1 in glycolysis, promoting CRC tumor growth.
Article
Biochemistry & Molecular Biology
Luigi Borzacchiello, Roberta Veglia Tranchese, Roberta Grillo, Roberta Arpino, Laura Mosca, Giovanna Cacciapuoti, Marina Porcelli
Summary: Recent research has found a correlation between dysregulated Notch and tumor metastasis in colorectal cancer (CRC). The study also showed that S-adenosyl-L-methionine (AdoMet) could act as a potential antimetastatic agent by modulating the expression of miRNA-34a/-34c/-449a and inhibiting the migration of CRC cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Chen Zhao, Xiaodong Li, Guantong Sun, Pengcheng Liu, Keyu Kong, Xuzhuo Chen, Fei Yang, Xiaoqing Wang
Summary: This study reveals the key role played by circFOXO3 in the progression of osteoarthritis (OA), showing that its overexpression can alleviate apoptosis of chondrocytes and promote the synthesis of extracellular matrix via activation of FOXO3 and autophagy.
CELL DEATH & DISEASE
(2022)
Article
Biochemistry & Molecular Biology
Catia Santos-Pereira, Juliana F. Rocha, Henrique S. Fernandes, Ligia R. Rodrigues, Manuela Corte-Real, Sergio F. Sousa
Summary: Lactoferrin is a bioactive milk protein with strong anticancer and antifungal activities, and its interaction with the proton pump V-ATPase leads to cell death. The study employed computational methods to elucidate the binding mechanism of lactoferrin to V-ATPase, revealing that lactoferrin inhibits ATP hydrolysis by binding to the cytosolic domain of V-ATPase.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Chemistry, Multidisciplinary
Razvan C. Cioc, Marc Crockatt, Jan C. van Der Waal, Pieter C. A. Bruijnincx
Summary: A hydrazine-mediated approach was used to produce renewable aromatics by Diels-Alder aromatization of biobased furfurals. The synthesis of aromatics using this method was selective, simple, and mild.
Article
Multidisciplinary Sciences
Zhen Zhang, Yun-xin Lu, Fangzhou Liu, Lingjie Sang, Chengyu Shi, Shaofang Xie, Weixiang Bian, Jie-cheng Yang, Zuozhen Yang, Lei Qu, Shi-yi Chen, Jun Li, Lu Yang, Qingfeng Yan, Wenqi Wang, Peifen Fu, Jianzhong Shao, Xu Li, Aifu Lin
Summary: It has been found that the lncRNA BREA2 stabilizes the Notch1 intracellular domain (NICD1) and promotes breast cancer metastasis, while WWP2 acts as an E3 ligase for NICD1 and suppresses breast cancer metastasis. BREA2 impairs the formation of the WWP2-NICD1 complex, leading to the activation of Notch signaling and lung metastasis. The loss of BREA2 sensitizes breast cancer cells to inhibition of Notch signaling and suppresses the growth of breast cancer patient-derived xenograft tumors, highlighting its therapeutic potential.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Review
Oncology
Jordan A. Harry, Mark L. Ormiston
Summary: Breast cancer is the most common cancer affecting women worldwide and is the second leading cause of cancer related death. Tumor angiogenesis plays a crucial role in the growth and metastasis of cancer, and while current anti-angiogenic therapies targeting Vascular Endothelial Growth Factor and other pathways have limitations and side effects, alternative pathways are being explored to find more effective treatments.
FRONTIERS IN ONCOLOGY
(2021)
Article
Biochemistry & Molecular Biology
G. Franciosa, G. Diluvio, F. Del Gaudio, M. V. Giuli, R. Palermo, P. Grazioli, A. F. Campese, C. Talora, D. Bellavia, G. D'Amati, Z. M. Besharat, C. Nicoletti, C. W. Siebel, L. Choy, A. Rustighi, G. Del Sal, I. Screpanti, S. Checquolo
Article
Oncology
Giulia Diluvio, Francesca Del Gaudio, Maria Valeria Giuli, Giulia Franciosa, Eugenia Giuliani, Rocco Palermo, Zein Mersini Besharat, Maria Gemma Pignataro, Alessandra Vacca, Giulia d'Amati, Marella Maroder, Claudio Talora, Carlo Capalbo, Diana Bellavia, Saula Checquolo
Article
Oncology
Maria Valeria Giuli, Giulia Diluvio, Eugenia Giuliani, Giulia Franciosa, Laura Di Magno, Maria Gemma Pignataro, Luca Tottone, Carmine Nicoletti, Zein Mersini Besharat, Giovanna Peruzzi, Maria Pelullo, Rocco Palermo, Gianluca Canettieri, Claudio Talora, Giulia D'Amati, Diana Bellavia, Isabella Screpanti, Saula Checquolo
Article
Oncology
Annamil Alvarez-Trotta, William Guerrant, Luisana Astudillo, Mohini Lahiry, Giulia Diluvio, Elena Shersher, Hugo Kaneku, David J. Robbins, Darren Orton, Anthony J. Capobianco
Summary: NADI-351 is the first specific small-molecule inhibitor that selectively disrupts Notch1 transcriptional complexes, demonstrating robust antitumor activity without inducing intestinal toxicity, providing a potential therapeutic approach for improved cancer treatment.
Article
Biochemistry & Molecular Biology
Sebastian K. J. Landor, Niina M. Santio, William B. Eccleshall, Valeriy M. Paramonov, Ellen K. Gagliani, Daniel Hall, Shao-Bo Jin, Kathe M. Dahlstrom, Tiina A. Salminen, Adolfo Rivero-Muller, Urban Lendahl, Rhett A. Kovall, Paivi J. Koskinen, Cecilia Sahlgren
Summary: Research shows that PIM kinases modulate the signaling output of different Notch paralogs through distinct mechanisms, promoting breast cancer tumorigenesis. Despite losing interaction with CSL after phosphorylation, Notch3 still maintains tumorigenic potential under estrogenic conditions.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Cell Biology
Elena Shersher, Mohini Lahiry, Annamil Alvarez-Trotta, Giulia Diluvio, David J. Robbins, Ramin Shiekhattar, Anthony J. Capobianco
Summary: This study identifies the INT complex as a novel co-factor in Notch-mediated transcription that together with NACK activates Notch target genes and leads to cancer cell proliferation.
CELL COMMUNICATION AND SIGNALING
(2021)
Article
Genetics & Heredity
Yi Kuang, Anna Pyo, Natanel Eafergan, Brittany Cain, Lisa M. Gutzwiller, Ofri Axelrod, Ellen K. Gagliani, Matthew T. Weirauch, Raphael Kopan, Rhett A. Kovall, David Sprinzak, Brian Gebelein
Summary: Notch signaling controls various developmental processes by regulating gene expression, with its dependent enhancers recruiting different complexes to mediate transcription through monomeric CSL sites and cooperative dimer sites called SPS. The way these enhancers bind different complexes can lead to significant differences in the level of target gene activation.
Article
Cell Biology
Laura Barreyro, Avery M. Sampson, Chiharu Ishikawa, Kathleen M. Hueneman, Kwangmin Choi, Mario A. Pujato, Somchai Chutipongtanate, Michael Wyder, Wendy D. Haffey, Eric O'Brien, Mark Wunderlich, Vighnesh Ramesh, Ellen M. Kolb, Cem Meydan, Yaseswini Neelamraju, Lyndsey C. Bolanos, Susanne Christie, Molly A. Smith, Madeline Niederkorn, Tomoya Muto, Santosh Kesari, Francine E. Garrett-Bakelman, Boris Bartholdy, Britta Will, Matthew T. Weirauch, James C. Mulloy, Zartash Gul, Stephen Medlin, Rhett A. Kovall, Ari M. Melnick, John P. Perentesis, Kenneth D. Greis, Elmar Nurmemmedov, William L. Seibel, Daniel T. Starczynowski
Summary: This study found that dysregulation of immune signaling pathways is associated with oncogenic immune signaling states in acute myeloid leukemia (AML) hematopoietic stem and progenitor cells. Inhibition of the ubiquitin-conjugating enzyme UBE2N disrupted oncogenic immune signaling and promoted cell death in AML cells while sparing normal cells. These findings highlight the potential of interfering with UBE2N function as a therapeutic strategy for AML.
SCIENCE TRANSLATIONAL MEDICINE
(2022)
Article
Genetics & Heredity
Ellen K. Gagliani, Lisa M. Gutzwiller, Yi Kuang, Yoshinobu Odaka, Phillipp Hoffmeister, Stefanie Hauff, Aleksandra Turkiewicz, Emily Harding-Theobald, Patrick J. Dolph, Tilman Borggrefe, Franz Oswald, Brian Gebelein, Rhett A. Kovall
Summary: Notch signaling pathway involves RBPJ variants associated with AOS, encoding proteins with compromised DNA binding activity but retained cofactor binding. This mechanism underlies the dysregulation of Notch target genes in AOS phenotypes.