Article
Cell Biology
Mark A. Aminzadeh, Mario Fournier, Akbarshakh Akhmerov, K. Candis Jones-Ungerleider, Jackelyn B. Valle, Eduardo Marban
Summary: This study found that administering heart-derived EVs orally, combined with casein, can exert beneficial bioactivity in vivo by improving muscle function in mice with muscular dystrophy. This approach enhances uptake and significantly expands the potential therapeutic applications, making it a viable treatment strategy.
JOURNAL OF EXTRACELLULAR VESICLES
(2021)
Article
Cardiac & Cardiovascular Systems
Achille Anselmo, Derk Frank, Laura Papa, Chiara Viviani Anselmi, Elisa Di Pasquale, Marta Mazzola, Cristina Panico, Francesca Clemente, Cristiana Soldani, Christina Pagiatakis, Rabea Hinkel, Ruth Thalmann, Reiner Kozlik-Feldmann, Michele Miragoli, Pierluigi Carullo, Marco Vacchiano, Antonio Chaves-Sanjuan, Nadia Santo, Maria Angela Losi, Matteo Carlo Ferrari, Annibale Alessandro Puca, Vincent Christiansen, Hatim Seoudy, Sandra Freitag-Wolf, Norbert Frey, Astrid Dempfle, Mark Mercola, Giovanni Esposito, Carlo Briguori, Christian Kupatt, Gianluigi Condorelli
Summary: Extracellular vesicles derived from cardiomyocytes, marked by CD172a, are increased in patients with aortic stenosis, ischaemic heart disease, and cardiomyopathy, and are influenced by cellular stress such as hypoxia. These vesicles can affect the cardiac function and may have prognostic value in aortic stenosis patients.
EUROPEAN HEART JOURNAL
(2021)
Article
Cell Biology
Stefan Vogt, Madhusudhan Reddy Bobbili, Gerhard Stadlmayr, Katharina Stadlbauer, Jorgen Kjems, Florian Ruker, Johannes Grillari, Gordana Wozniak-Knopp
Summary: Research has shown that by using the CD81 protein to recognize target antigens, the targeted delivery capability of EVs can be enhanced, and this has been validated in mammalian cells. Mutant sequences are reformatted into full-length CD81 and expressed in EVs produced by HeLa cells, showing improved uptake by specific antigens.
JOURNAL OF EXTRACELLULAR VESICLES
(2021)
Article
Multidisciplinary Sciences
Luize G. Lima, Sunyoung Ham, Hyunku Shin, Edna P. Z. Chai, Erica S. H. Lek, Richard J. Lobb, Alexandra F. Muller, Suresh Mathivanan, Belinda Yeo, Yeonho Choi, Belinda S. Parker, Andreas Moller
Summary: Exosomes secreted by cancer cells play a crucial role in metastasis, with cytokines in the tumor microenvironment binding to exosomes and promoting their uptake by specific cell lineages and organs to enhance metastatic dissemination. This interaction between exosomes and cytokines is a key determinant of cancer progression.
NATURE COMMUNICATIONS
(2021)
Article
Medicine, Research & Experimental
Chao Chen, Li Zhang, Zhengyi Ruan
Summary: This study investigates the regulatory mechanism of GATA3 in tumor-associated macrophages derived extracellular vesicles (EVs) and its role in immune escape and chemotherapy resistance of ovarian cancer (OC) cells.
MOLECULAR PHARMACEUTICS
(2023)
Review
Engineering, Biomedical
Qingpeng Xie, Yujia Hao, Na Li, Haoyue Song, Xiaohang Chen, Zilan Zhou, Jia Wang, Yuan Zhang, Huifei Li, Pengcheng Han, Xing Wang
Summary: Extracellular vesicles (EVs) are nanosized membrane vesicles that play a role in intercellular communication. However, their low uptake rate and rapid clearance limit their therapeutic applications. Engineering strategies can enhance EV uptake and overcome these limitations, opening up potential clinical uses.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Chemistry, Physical
Ziyuan Wang, Haisheng Zhou, Haoran Yu, Zhongji Pu, Jinling Xu, Hongyu Zhang, Jianping Wu, Lirong Yang
Summary: This study investigates the substrate recognition mechanism of glutamate dehydrogenase (GluDH) and develops a computational engineering strategy to redesign the binding pocket, leading to the synthesis of noncanonical amino acids. Tailor-made variants were created to improve the specific activities for producing high-value products. The computational enzyme redesign strategy shows promise in enhancing the production of noncanonical amino acids.
Article
Neurosciences
Zhihui Zhu, Zainuddin Quadri, Simone M. Crivelli, Ahmed Elsherbini, Liping Zhang, Priyanka Tripathi, Haiyan Qin, Emily Roush, Stefka D. Spassieva, Mariana Nikolova-Karakashian, Timothy S. McClintock, Erhard Bieberich
Summary: Deficiency of nSMase2 improves memory in adult mice and prevents oxidative stress-induced elevation of ceramide and secretion of exosomes by astrocytes that suppress neuronal function.
MOLECULAR NEUROBIOLOGY
(2022)
Review
Engineering, Chemical
Zhi Hao Kwok, Chenghao Wang, Yang Jin
Summary: Emerging evidence suggests that extracellular vesicles (EVs) play a significant role in modulating human diseases, but further research is needed to understand their transportation and uptake mechanisms. Understanding the specificity of EV transport is crucial for the development of EVs as valuable diagnostics and therapeutics.
Article
Biotechnology & Applied Microbiology
Sabrina Picciotto, Pamela Santonicola, Angela Paterna, Estella Rao, Samuele Raccosta, Daniele Paolo Romancino, Rosina Noto, Nicolas Touzet, Mauro Manno, Elia Di Schiavi, Antonella Bongiovanni, Giorgia Adamo
Summary: Extracellular vesicles (EVs) are lipid membrane nano-sized vesicles secreted by various cell types for intercellular communication. Nanoalgosomes, a subtype of EVs derived from microalgae, show potential as nanocarriers of bioactive compounds. In this study, it was found that nanoalgosomes can be actively uptaken in vitro by human cells and in vivo by C. elegans cells, offering potential applications for theranostics.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biophysics
Vasiliy S. Chernyshev, Daniil Nozdriukhin, Roman Chuprov-Netochin, Ekaterina Tsydenzhapova, Marina Novoselova, Dmitry Gorin, Alexey Yashchenok
Summary: sEVs, with their exceptional properties, have attracted significant interest in therapeutic and diagnostic applications. Researchers have found that deposition of sEVs onto polyelectrolyte-coated silica microparticles can enhance their uptake by cells, highlighting the important role of sEV membrane receptors in the interaction with cells.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Article
Cell Biology
Julia Solana-Balaguer, Nuria Martin-Flores, Pol Garcia-Segura, Genis Campoy-Campos, Leticia Perez-Sisques, Almudena Chicote-Gonzalez, Joaquin Fernandez-Irigoyen, Enrique Santamaria, Esther Perez-Navarro, Jordi Alberch, Cristina Malagelada
Summary: This study investigates the transfer of RTP801 toxicity via extracellular vesicles (EVs), and its impact on neuronal death and morphology. Results indicate that RTP801-induced toxicity is transferred to neurons via EVs, leading to apoptosis and impairing neuron morphology complexity. Conversely, EVs derived from neurons where RTP801 was silenced show improved arborization in recipient neurons.
JOURNAL OF EXTRACELLULAR VESICLES
(2023)
Article
Pharmacology & Pharmacy
Youchao Deng, Xiaosheng Song, Iredia D. Iyamu, Aiping Dong, Jinrong Min, Rong Huang
Summary: In this study, we reported the discovery of a noncanonical SAH surrogate YD1113 that selectively inhibits PRMT3/4/5. Crystal structures revealed a unique hydrophobic binding pocket induced by YD1113 in PRMT3/4. Furthermore, YD1113 could be modified to form a bisubstrate analogue YD1290, which exhibited potent and selective inhibition to type I PRMTs.
ACTA PHARMACEUTICA SINICA B
(2023)
Article
Pharmacology & Pharmacy
Tuan Thach Pham, Huan Chen, Phuong Hoang Diem Nguyen, Migara Kavishka Jayasinghe, Anh Hong Le, Minh T. N. Le
Summary: Extracellular vesicles derived from red blood cells (RBCEVs) can release therapeutic RNA/DNA cargos at late endosomes and lysosomes, allowing the cargos to interact with cytoplasmic targets and lead to tumor suppression in vitro and in a murine model of acute myeloid leukemia without significant toxicity. Surface functionalization of RBCEVs with an anti-human CXCR4 antibody enhances gene silencing efficiency by facilitating specific uptake by CXCR4+ leukemic cells. These findings provide insights into the mechanisms of cellular uptake and endosomal escape routes of nucleic acid cargos delivered by RBCEVs and have important implications for improving the RBCEV-based delivery system.
PHARMACOLOGICAL RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Joelle Noriko Galang, Yefeng Shen, Ulrike Koitzsch, Xiaojie Yu, Hannah Eischeid-Scholz, Daniel Bachurski, Tilman T. Rau, Christina Neppl, Marco Herling, Bianca Bulimaga, Elena Vasyutina, Michal R. Schweiger, Reinhard Buettner, Margarete Odenthal, Maria M. Anokhina
Summary: In this study, circLSD1-RNAs were found to participate in the intercellular communication of lung cancer cells with the tumor environment. They are primarily located in the cytoplasm and are released from lung cancer and non-cancer cells by extracellular vesicles and ribonucleoprotein complexes, respectively. Importantly, they are differently taken up by different cell types.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Anissa Viveiros, Vaibhavi Kadam, John Monyror, Luis Carlos Morales, Desmond Pink, Aja M. Rieger, Simonetta Sipione, Elena Posse de Chaves
Summary: Extracellular vesicles (EVs) play important roles in physiological functions and health and disease. This study presents a method for in-cell EV labeling using fluorescent lipids and direct analysis of lipid-labeled EVs in the conditioned medium by imaging flow cytometry (IFC). The optimized protocol reduces sample processing and loss, enabling improved detection of quantitative changes in EV secretion and subpopulations.
Article
Chemistry, Multidisciplinary
Duong T. Bui, Zhixiong Li, Pavel I. Kitov, Ling Han, Elena N. Kitova, Marlene Fortier, Camille Fuselier, Philippine Granger Joly de Boissel, David Chatenet, Nicolas Doucet, Stephen M. Tompkins, Yves St-Pierre, Lara K. Mahal, John S. Klassen
Summary: Electrospray ionization mass spectrometry (ESI-MS) is a powerful label-free assay for detecting noncovalent biomolecular complexes and quantifying binding thermochemistry. However, the assumption that the ion signals of free and bound species in ESI-MS measurements can quantitatively reflect their relative concentrations is only valid when their response factors are similar.
ACS CENTRAL SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Ilhan Tomris, Luca Unione, Linh Nguyen, Pouya Zaree, Kim M. Bouwman, Lin Liu, Zeshi Li, Jelle A. Fok, Maria Rios Carrasco, Roosmarijn van der Woude, Anne L. M. Kimpel, Mirte W. Linthorst, Sinan E. Kilavuzoglu, Enrico C. J. M. Verpalen, Tom G. Caniels, Rogier W. Sanders, Balthasar A. . Heesters, Roland J. Pieters, Jesu's Jimenez-Barbero, John S. Klassen, Geert-Jan Boons, Robert P. de Vries
Summary: SARS-CoV-2 viruses use ACE2 as a functional receptor through their spike protein. The NTD of SARS-CoV-2 beta variant strain is capable of binding to Vero E6 cells and has a specific preference for 9-O-acetylated structures, suggesting a potential dual-receptor functionality of the S1 domain.
ACS CHEMICAL BIOLOGY
(2023)
Article
Nanoscience & Nanotechnology
Ming Xia, Frank Vago, Ling Han, Pengwei Huang, Linh Nguyen, Geert-Jan Boons, John S. Klassen, Wen Jiang, Ming Tan
Summary: In this study, the aTSR domain of the CSP was characterized and found to bind to heparan sulfate glycans, making it an important functional domain and a potential vaccine target. Fusion of aTSR with norovirus VP1's S domain resulted in the formation of highly immunogenic nanoparticles that elicited specific antibodies. These findings suggest that the aTSR nanoparticle could be a promising vaccine candidate for preventing and treating malaria.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2023)
Article
Chemistry, Multidisciplinary
Duong T. Bui, James Favell, Elena N. Kitova, Zhixiong Li, Kelli A. McCord, Edward N. Schmidt, Fahima Mozaneh, Mohamed Elaish, Amr El-Hawiet, Yves St-Pierre, Tom C. Hobman, Matthew S. Macauley, Lara K. Mahal, Morris R. Flynn, John S. Klassen
Summary: We introduce a breakthrough screening technology called Concentration-Independent (COIN)-nMS, which enables the quantitative screening of natural glycan libraries and measurement of absolute ligand affinities for glycan-binding proteins. This technology exploits slow mixing of solutions inside a nanoflow electrospray ionization emitter to determine the affinities of GBP-glycan interactions. The results demonstrate the versatility of COIN-CaR-nMS in discovering new ligands, precisely measuring their affinities, and uncovering fine specificities, such as sialoglycan binding properties of the SARS-CoV-2 receptor binding domain and recognition of monosialylated hybrid and biantennary N-glycans.
ACS CENTRAL SCIENCE
(2023)
Article
Chemistry, Analytical
Zhixiong Li, Duong T. Bui, Yanxiang Shao, Elena N. Kitova, Stephanie White, Danny Vesprini, Stanley K. Liu, Lara K. Mahal, Hon S. Leong, John S. Klassen
Summary: The PSA test is commonly used for prostate cancer screening but it may result in misdiagnosis. Increased levels of α2-3-linked N-acetylneuraminic acid in the N-glycan sialylation of PSA have been identified as a potential biomarker for clinically significant prostate cancer. A native mass spectrometry approach was developed to quantify α2-3-linked N-acetylneuraminic acid levels in PSA extracted from blood serum, which can discriminate between clinically significant prostate cancer and low-grade prostate cancer.
ANALYTICAL CHEMISTRY
(2023)
Article
Biology
Khushali Patel, Jasmine Nguyen, Sumaiyah Shaha, Amy Brightwell, Wendy Duan, Ashley Zubkowski, Ivan K. Domingo, Meghan Riddell
Summary: The syncytiotrophoblast, an epithelial cell in the human placenta, acts as a barrier and exchange interface between the mother and fetus. Dysfunction of syncytiotrophoblasts is observed in pregnancy pathologies, such as preeclampsia. Our study shows that a loss of aPKC activity or expression induces programmed necrosis in syncytiotrophoblasts through gasdermin-E. This suggests that aPKCs play a role in maintaining the function of syncytiotrophoblasts and that pro-inflammatory cytokines can lead to cell death at the maternal-fetal interface.
LIFE SCIENCE ALLIANCE
(2023)