Article
Biochemistry & Molecular Biology
Wen-Jing Zhong, Tian Liu, Hui-Hui Yang, Jia-Xi Duan, Jin-Tong Yang, Xin-Xin Guan, Jian-Bing Xiong, Yan-Feng Zhang, Chen-Yu Zhang, Yong Zhou, Cha-Xiang Guan
Summary: The activation of the triggering receptor expressed on myeloid cells-1 (TREM-1) can potentiate acute lung injury (ALI) and induce inflammation, but the underlying mechanism is unclear. This study found that blocking TREM-1 can attenuate the activation of NOD-, LRR- and pyrin domain-containing 3 (NLRP3) inflammasome and glycolysis in LPS-induced ALI mice. Furthermore, TREM-1 activation enhances glycolysis and inhibits oxidative phosphorylation in macrophages, and this effect is dependent on the hypoxia-inducible factor-1a (HIF-1 alpha)/mTOR/glycolysis pathway. Inhibiting mTOR or HIF-1 alpha can suppress TREM-1-induced metabolic reprogramming and NLRP3/caspase-1 activation. These findings reveal a novel mechanism of TREM-1-mediated NLRP3 inflammasome activation through the mTOR/HIF-1 alpha/glycolysis pathway.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2023)
Article
Engineering, Biomedical
Chaowang Huang, Qianyi You, Jing Xu, Di Wu, Huaping Chen, Yuhang Guo, Jiancheng Xu, Mingdong Hu, Hang Qian
Summary: This study proposes and demonstrates an anti-inflammatory dual therapy strategy that uses self-assembled DNA nanostructures as nontoxic, programmable delivery vehicles, achieving anti-inflammatory effects by regulating macrophage polarization and promoting macrophage autophagy.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Cell Biology
Hui-Ling Jiang, Hui-Hui Yang, Yu-Biao Liu, Chen-Yu Zhang, Wen-Jing Zhong, Xin-Xin Guan, Ling Jin, Jie-Ru Hong, Jin-Tong Yang, Xiao-Hua Tan, Qing Li, Yong Zhou, Cha-Xiang Guan
Summary: This study investigated the role of L-OPA1 deficiency-mediated mitochondrial dysfunction in the necroptosis of alveolar epithelial cells (AECs). The findings revealed that inhibition of L-OPA1 aggravated the pathological injury, inflammatory response, and necroptosis in the lungs of LPS-induced acute lung injury mice. In vitro experiments demonstrated that inhibition of L-OPA1 induced necroptosis of AECs, while activation of L-OPA1 alleviated necroptosis of AECs under the LPS challenge.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Medicine, Research & Experimental
Liangjun Xia, Chunli Zhang, Nianyin Lv, Zihao Liang, Tonghui Ma, Haibo Cheng, Youbing Xia, Liyun Shi
Summary: AdMSC-Exos effectively donate mitochondrial components to improve macrophage mitochondrial integrity and oxidative phosphorylation, leading to the restoration of metabolic and immune homeostasis in airway macrophages and alleviating lung inflammatory pathology.
Article
Biochemistry & Molecular Biology
Hang Zhu, Ying Tan, Wenjun Du, Yang Li, Sam Toan, David Mui, Feng Tian, Hao Zhou
Summary: In the context of cardiac ischemia-reperfusion injury, PGAM5 plays a role in driving cardiomyocyte necroptosis by regulating mitochondrial quality control, while also suppressing necroptosis without preventing apoptosis, leading to improved heart function and reduced inflammation response.
Article
Physiology
Sara Siskind, Fangming Zhang, Max Brenner, Ping Wang
Summary: Circulating eCIRP and eCIRP-induced activation of the TREM-1 receptor play an important role in the development of AKI. Mice deficient in TREM-1 have attenuated AKI and reduced endothelial cell activation after injection of rmCIRP. Inhibition of TREM-1 with M3 attenuates HRGEC activation after eCIRP stimulation.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Medicine, Research & Experimental
Yan Ding, Lei Ma, Limin He, Quanxiao Xu, Zhuang Zhang, Zhen Zhang, Xinping Zhang, Rui Fan, Wenjun Ma, Ya 'nan Sun, Baile Zhang, Wentai Li, Yao Zhai, Jiandong Zhang
Summary: This study demonstrates the preventive effect of crocetin on radiation-induced lung injury, primarily achieved by inhibiting necroptosis. This work provides a new strategy for the prevention of lung radiation injury using extracts from Chinese herbal medicine.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Biology
Parker S. Woods, Lucas M. Kimmig, Kaitlyn A. Sun, Angelo Y. Meliton, Obada R. Shamaa, Yufeng Tian, Renguel Cetin-Atalay, Willard W. Sharp, Robert B. Hamanaka, Goekhan M. Mutlu
Summary: Cellular metabolism plays a crucial role in regulating macrophage function. Changes in local oxygen concentration, such as those seen in conditions like acute respiratory distress syndrome (ARDS), can impact the metabolism and function of tissue-resident alveolar macrophages (TR-AMs), leading to their progressive loss which correlates with disease severity and mortality. Targeting cellular metabolism, particularly under hypoxic conditions, may hold therapeutic potential in improving outcomes in diseases characterized by acute inflammation.
Article
Pharmacology & Pharmacy
Rui-wen Mao, Shan-ping He, Jun-gang Lan, Wu-zheng Zhu
Summary: The study suggests that honokiol can prevent cisplatin-induced acute kidney injury by preserving mitochondrial integrity and function.
BRITISH JOURNAL OF PHARMACOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Lijuan Xu, Ye Tu, Jiao Li, Wannian Zhang, Zhibin Wang, Chunlin Zhuang, Lei Xue
Summary: This study developed a series of cyclohexanone-containing benzothiazole analogues with excellent dual-targeting RIPK1 and RIPK3 activity and anti-necroptosis activity. Compound 41, in particular, showed improved selectivity and anti-necroptosis activity compared to the original compound SZM594. It was able to inhibit TNF-alpha expression in a cell model and significantly alleviate LPS-induced ALI in a mouse model. These compounds hold promise as lead structures for the discovery of novel protective agents for ALI.
CHINESE CHEMICAL LETTERS
(2022)
Article
Critical Care Medicine
Maomao Sun, Zhenhua Zeng, Gege Xu, Sheng An, Zhiya Deng, Ran Cheng, Yi Yao, Junjie Wu, Hongbin Hu, Qiaobing Huang, Jie Wu
Summary: This study revealed that the mitochondrial dynamics of alveolar macrophages (AMs) are imbalanced in sepsis and acute lung injury (ALI)/acute respiratory distress syndrome (ARDS), characterized by impaired mitochondrial fusion, increased fission, and mitochondrial cristae remodeling. Modulating mitochondrial dynamics by suppressing excessive fission or promoting fusion can inhibit the polarization of AMs into a proinflammatory phenotype and attenuate sepsis-induced ALI.
Article
Immunology
Xuehui Li, Yongting Zhang, Jinping Wang, Yuyu Li, Yuchong Wang, Fan Shi, Liang Hong, Lanjuan Li, Hongyan Diao
Summary: In this study, Z-Val-Ala-DL-Asp-fluoromethylketone (zVAD) was found to alleviate ConA-induced liver injury in mice by increasing macrophage sensitivity to necroptosis through IL-10-induced TNFR1 expression. This research sheds new light on the treatment of autoimmune hepatitis by inducing macrophage necroptosis with zVAD.
JOURNAL OF AUTOIMMUNITY
(2022)
Article
Biochemistry & Molecular Biology
Zhu-Lin Luo, Hong-Yu Sun, Xiao-Bo Wu, Long Cheng, Jian-Dong Ren
Summary: The study demonstrated that EGCG can significantly attenuate lung injury during acute pancreatitis by inhibiting NLRP3 inflammasome activation. The mechanism is mainly attributed to the elimination of mtROS and its oxidative product ox-mtDNA, thus providing protection against AP-associated lung injury.
Article
Immunology
Kavitha Sedmaki, Kalyani Karnam, Pravesh Sharma, Ashutosh Mahale, Ganesh Routholla, Balaram Ghosh, Onkar Prakash Kulkarni
Summary: This study highlights the role of HDAC6 in regulating the tubulin-mediated secretion of IL-1 beta and RIP kinase mediated necroptosis in acute oxalate nephropathy.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Article
Plant Sciences
Yan-Ru Cui, Fei Qu, Wen-Jing Zhong, Hui-Hui Yang, Jie Zeng, Jun-Hao Huang, Jie Liu, Ming-Yue Zhang, Yong Zhou, Cha-Xiang Guan
Summary: This study validated the beneficial effects of Alo in LPS-induced acute lung injury (ALI), suggesting that Alo may be a new drug candidate against ALI. Alo attenuated lung injuries, reduced inflammation and oxidative stress, and reversed LPS-induced necroptosis.