Colloidal gold-McAb probe-based rapid immunoassay strip for simultaneous detection of fumonisins in maize

BACKGROUNDFumonisins are a kind of toxic and carcinogenic mycotoxin. A rapid immunochromatographic test strip has been developed for simultaneous detection of fumonisin B-1, B-2 and B-3 (FB1, FB2 and FB3) in maize based on colloidal gold-labelled monoclonal antibody (McAb) against FB1 probe. RESULTSThe anti-FB1 McAb (2E11-H3) was produced through immunisation and cell fusion, and identified as high affinity, specificity and sensitivity. The cross-reaction ratios with fumonisin B-2 and B-3 were accordingly 385% and 72.4%, while none with other analogues. The colloid gold-labelled anti-FB1 McAb probe was successfully prepared and used for establishing the immunochromatographic strip. The test strip showed high sensitivity and specificity, the IC50 for FB1 was 58.08 ng mL(-1), LOD was 11.24 ng mL(-1), calculated from standard curve. Moreover, the test strip exhibited high cross-reactivity with FB2 and FB3, and could be applied to the simultaneous detection of FBs (FB1:FB2:FB3 = 12:4:1) in maize sample with high accuracy and precision. The average recoveries of FBs in maize ranged from 90.42% to 95.29%, and CVs were 1.25-3.77%. The results of the test strip for FBs samples showed good correlation with high-performance liquid chromatography analysis. CONCLUSIONThe immunochromatographic test strip could be employed in the rapid simultaneous detection of FB1, FB2 and FB3 in maize samples on-site. (c) 2016 Society of Chemical Industry