Article
Chemistry, Applied
Keysson V. Fernandes, Elisa D. C. Cavalcanti, Eliane P. Cipolatti, Erika C. G. Aguieiras, Martina C. C. Pinto, Fernanda A. Tavares, Priscila R. da Silva, Roberto Fernandez-Lafuente, Sara Arana-Pena, Jose Carlos Pinto, Charles L. B. Assuncao, Jose Andre C. da Silva, Denise M. G. Freire
Summary: This study successfully synthesized biolubricants from by-products of soybean oil processing using the lipase from Candida rugosa, showing high conversion rates and improved efficiency through immobilization. The products demonstrated good lubricant properties, indicating a potential environmentally friendly alternative to petroleum-based lubricants.
Article
Agriculture, Multidisciplinary
Yifei Zhang, Guangzheng Ma, Shushu Wang, Binbin Nian, Yi Hu
Summary: This study achieved efficient immobilization of Candida rugosa lipase on commercial macroporous resins, resulting in an efficient catalyst for the synthesis of phytosterol esters. A solvent-free strategy using oleic acid as the esterification reaction medium yielded a high yield of ester synthesis. This immobilization strategy provides new possibilities for the high-efficiency production of pine sterol esters and other food source nutrients.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2023)
Article
Biotechnology & Applied Microbiology
Shushu Wang, Shan Li, Runtang Liu, Wei Zhang, Huajin Xu, Yi Hu
Summary: In this study, Candida rugosa lipase (CRL) was activated and covalently immobilized on a nanocomposite material using surfactants. The nanocomposite material, fabricated by combining magnetic nanoparticles with chitosan, proved to be an ideal support for enzyme immobilization. The immobilized CRL showed improved stability and reusability.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Nanoscience & Nanotechnology
Yongfa Cheng, Oi-Ming Lai, Chin-Ping Tan, Worawan Panpipat, Ling-Zhi Cheong, Cai Shen
Summary: Immobilization can improve the stability and reusability of lipases, but may also lead to conformational changes that decrease catalytic activity. In this study, grafting proline onto UIO-66 crystals for immobilization of Candida rugosa lipase showed enhanced enzyme activity and better recycling capabilities, indicating improved catalytic efficiency.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Biochemistry & Molecular Biology
Elif Ozyilmaz, Ozge Caglar
Summary: In this study, a mechanochemical technique was used to immobilize enzymes, with two different zinc-based MOF types being preferred. It was found that one of the MOFs (Zn-MOF-74) had six times higher catalytic activity than the other (ZIF-8). Furthermore, Zn-MOF-74 demonstrated better enantioselectivity.
PROCESS BIOCHEMISTRY
(2023)
Article
Materials Science, Multidisciplinary
Nengwen Gao, Jing Liu, Xiaoli Wang, Yu Zhang
Summary: This study investigates the immobilization of lipase on ceramic membranes using modification and cross-linking methods. The immobilized lipases show higher catalytic activity and stability, making them suitable for applications in high-temperature and alkaline environments.
JOURNAL OF MATERIALS SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Evan A. Bisirri, Thaiesha A. Wright, Daniel K. Schwartz, Joel L. Kaar
Summary: Protein-polymer conjugation is a useful approach to improve protein stability and performance, but polymer-polymer interactions can also affect the properties of polymer-modified proteins. This study demonstrates that by adjusting the ratio of polymers, the productivity of lipase can be optimized, although this may lead to a trade-off between activity and stability.
Article
Biology
Prasanna J. Patil, Subodh A. Kamble, Maruti J. Dhanavade, Xin Liang, Chengnan Zhang, Xiuting Li
Summary: This study used molecular modeling approaches to investigate the interaction between CRL and the specific MOF ZIF-8. The results of molecular docking analysis showed that ZIF-8 was located at the active site pocket of CRL and formed hydrogen bonds with specific amino acids. The MD simulation analysis demonstrated the stability of the CRL-ZIF-8 docked complex and the maintenance of all interactions throughout the simulation. These findings contribute to the understanding of the molecular mechanisms and potential applications of immobilized CRL for various industries.
Article
Chemistry, Multidisciplinary
Miaojia Song, Xiaohui Di, Yu Zhang, Yongming Sun, Zhongming Wang, Zhenhong Yuan, Ying Guo
Summary: This study investigated the effects of enzyme loading, alcohol/acid ratio, and temperature on the yield of methyl levulinate through a kinetic model. The results showed that enzyme loading increases the rate constants of esterification/hydrolysis, while temperature elevation increases both the rate constants and the equilibrium constant. The application of binary regression analysis was successful in studying the enzymatic esterification kinetics.
Article
Materials Science, Multidisciplinary
Elif Ozyilmaz, Ozge Caglar, Idris Sargin, Gulsin Arslan
Summary: The study successfully prepared a new support material for encapsulating Candida rugosa lipase by combining edible mushroom-derived CQDs and ZIF-8 crystals, with higher enzyme loading efficiency compared to in-situ encapsulation. Structural, morphological, and spectrophotometric analyses showed that CQDs and the enzyme were physically entrapped within the three-dimensional structures of ZIF-8.
MATERIALS TODAY COMMUNICATIONS
(2022)
Article
Chemistry, Physical
Yupei Jian, Yilei Han, Zhongwang Fu, Meng Xia, Guoqiang Jiang, Diannan Lu, Jianzhong Wu, Zheng Liu
Summary: This study investigates the conformational dynamics of enzymes in organic solvents and their impact on catalytic kinetics through a combination of experimental and simulation approaches. The results demonstrate that conjugating enzymes with soluble polymers enhances enzyme flexibility and improves catalytic efficiency. The simulations further support the hypothesis that polymer motion enhances enzyme dynamics.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2022)
Article
Chemistry, Physical
Nurfadhila Nasya Ramlee, Rosli Md Illias, Roshanida A. Rahman, Susilawati Toemen, Rangabhashiyam Selvasembian, Rabi'atul Adawiyah Ahmad, Nor Hasmaliana Abdul Manas, Nur Izyan Wan Azelee
Summary: Enzyme immobilization on inorganic materials is gaining more attention due to the potential characteristics of high surface area-to-volume ratios, increasing enzyme loading efficiency. In this study, Candida rugosa lipase was successfully immobilized on a metal oxide hybrid support, showing high thermal stability and wide pH stability range. The immobilized enzyme also demonstrated high tolerance in solvents compared to free enzyme.
Review
Chemistry, Multidisciplinary
Ailada Charoenpol, Daniel Crespy, Albert Schulte, Wipa Suginta
Summary: Several enzymes can convert chitin and its derivative, chitosan, into low-molecular weight sugars known as chitooligosaccharides (COSs), which have biological activity and potential applications in biomedical, pharmaceutical, and biotechnological fields. Enzyme immobilization on carrier particles can improve enzyme stability and recovery.
Article
Spectroscopy
Shiyi Chen, Mengli Ma, Juan Peng, Xiaogang He, Qian Wang, Ganghui Chu
Summary: A novel approach based on nearinfrared spectroscopy (NIRs) was developed for predicting the enzyme activity of CRL/ZIF-8. The NIR model was established by linking the enzyme activity data with the NIR spectra of the samples. The model showed a strong relationship between NIRs and CRL/ZIF-8 enzyme activity, providing a simple and rapid method for quantifying the enzyme activity.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Biochemistry & Molecular Biology
Zeping Lu, Mei Chen, Tongtong Jin, Binbin Nian, Yi Hu
Summary: Ionic liquids (ILs) are used as chemical modifiers to improve the efficiency, activity, and stability of enzymes. This study investigated the impact of ILs with different chain lengths on the enzymatic properties of Candida antarctica lipase B (CALB) and found that ILs significantly influenced CALB's absorption orientation. Molecular dynamics simulations showed that ILs with suitable chain lengths formed strong interactions with CNTs and ILs, stabilizing CALB's open state and improving its activity and stability. This study provides insights into the design of green modifiers for better immobilized enzymes and lays the groundwork for future research on IL modifiers.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Environmental Sciences
Aleksandar Stojsavljevic, Jovana Jagodic, Ljiljana Vujotic, Slavica Borkovic-Mitic, Zorica Rasic-Milutinovic, Dragana Jovanovic, Marija Gavrovic-Jankulovic, Dragan Manojlovic
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2020)
Article
Biochemical Research Methods
Dusan Velickovic, Guanshi Zhang, Dejan Bezbradica, Arunima Bhattacharjee, Ljiljana Pasa-Tolic, Kumar Sharma, Theodore Alexandrov, Christopher R. Anderton
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2020)
Review
Chemistry, Multidisciplinary
Noelia Losada-Garcia, Ivan Rodriguez-Oliva, Milica Simovic, Dejan Bezbradica, Jose M. Palomo
Article
Chemistry, Multidisciplinary
Marija Corovic, Ana Milivojevic, Milica Simovic, Katarina Banjanac, Rada Pjanovic, Dejan Bezbradica
SUSTAINABLE CHEMISTRY AND PHARMACY
(2020)
Article
Environmental Sciences
Aleksandar Stojsavljevic, Branislav Rovcanin, Jovana Jagodic, Danijela Draskovic Radojkovic, Ivan Paunovic, Marija Gavrovic-Jankulovic, Dragan Manojlovic
ENVIRONMENTAL RESEARCH
(2020)
Article
Biochemistry & Molecular Biology
Aleksandar Stojsavljevic, Branislav Rovcanin, Jovana Jagodic, Durda Krstic, Ivan Paunovic, Marija Gavrovic-Jankulovic, Dragan Manojlovic
Summary: The study revealed changes in trace elements in thyroid disease patients and suggested that certain element ratios could serve as blood markers for disease discrimination. Significant differences in the levels of certain elements in pathological blood samples compared to control samples were found, indicating the potential for new circulating screening markers.
BIOLOGICAL TRACE ELEMENT RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Jovana Jagodic, Branislav Rovcanin, Ivan Paunovic, Milan Jovanovic, Marija Gavrovic-Jankulovic, Dragan Manojlovic, Aleksandar Stojsavljevic
Summary: This study provided insight into the baseline status of trace metals in healthy adrenal tissues and adenomatous adrenal tissues. Adenomatous tissues showed altered trace metal contents compared to healthy tissues, with significant findings related to essential elements and lead. Nodular diameter above 4 cm had the most significant effect on changes in elemental composition.
JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Zorana Lopandic, Luka Dragacevic, Dragan Popovic, Uros Andjelkovic, Rajna Minic, Marija Gavrovic-Jankulovic
Summary: This study designed a BanLec-eGFP chimera for studying the glycosylation structure and function of different microorganisms, successfully expressed and confirmed its structure using molecular modeling and molecular mechanics techniques.
Article
Biochemistry & Molecular Biology
Zorana Lopandic, Isidora Protic-Rosic, Aleksandra Todorovic, Sofija Glamoclija, Marija Gnjatovic, Danica Cujic, Marija Gavrovic-Jankulovic
Summary: This study demonstrated the immunoreactivity of a designed M protein of the SARS-CoV-2 virus with IgM and IgG antibodies in serum samples from COVID-19 convalescents. The ELISA results showed over 93% positivity for IgM detection and over 96% positivity for specific IgG detection to the M protein in COVID-19 sera. Based on its ability to provoke an immune response and strong antigenic properties, the M protein has the potential for diagnostic purposes and vaccine design.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Food Science & Technology
Katarina Katic, Katarina Banjanac, Milica Simovic, Marija Corovic, Ana Milivojevic, Aleksandar Marinkovic, Dejan Bezbradica
Summary: The study found that FNS and its derivatives are suitable for immobilizing commercial protease preparations, resulting in higher hydrolytic activities and yields when hydrolyzing sunflower meal protein isolate.
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Andrijana Nesic, Milena Cavic, Milica Popovic, Marija Gavrovic-Jankulovic
Summary: The study evaluated the activation of Act d 1 in cell culture medium and its impact on the gene expression of pro-inflammatory cytokines in two model systems.
MOLECULAR BIOLOGY REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Luka Dragacevic, Zorana Lopandic, Marija Gavrovic-Jankulovic, Irena Zivkovic, Veljko Blagojevic, Natalija Polovic, Rajna Minic
Summary: The surface of microorganisms is covered with carbohydrates, making them unique glycan probes. Lectins can bind to these probes, which can be used for selecting microorganisms or novel lectins. The enzyme-linked lectin sorbent assay (ELLSA) is a reliable and convenient method for high-throughput analysis, which can be used for profiling microbial glycan structures or testing novel lectins.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Isidora Protic-Rosic, Andrijana Nesic, Ivana Lukic, Radmila Miljkovic, Dragan M. Popovic, Marina Atanaskovic-Markovic, Marijana Stojanovic, Marija Gavrovic-Jankulovic
Summary: The study introduces a newly designed recombinant chimera with potential immunomodulatory effects for the treatment of allergic diseases, showing promising implications for allergen-specific immunotherapy.
MOLECULAR IMMUNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Ana Milivojevic, Marija Corovic, Milica Simovic, Katarina Banjanac, Dejan Bezbradica
BIOCHEMICAL ENGINEERING JOURNAL
(2020)
Article
Chemistry, Multidisciplinary
Jelena Bebic, Katarina Banjanac, Jelena Rusmirovic, Marija Corovic, Ana Milivojevic, Milica Simovic, Aleksandar Marinkovic, Dejan Bezbradica