Article
Biochemistry & Molecular Biology
Caterina Visconte, Chiara Fenoglio, Maria Serpente, Paola Muti, Andrea Sacconi, Marta Rigoni, Andrea Arighi, Vittoria Borracci, Marina Arcaro, Beatrice Arosio, Evelyn Ferri, Maria Teresa Golia, Elio Scarpini, Daniela Galimberti
Summary: This study isolated total EVs from plasma and characterized the miRNA contents in AD patients. The results showed widespread deregulation of specific miRNAs in plasma EVs, some of which were already known to be associated with neurological pathologies. Further validation analysis confirmed the upregulation of certain miRNAs in prodromal AD patients, suggesting their involvement in the early progression of AD.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Physiology
Atanaska Ivanova Doncheva, Silvana Romero, Manuel Ramirez-Garrastacho, Sindre Lee, Kristoffer J. Kolnes, Daniel Steensen Tangen, Thomas Olsen, Christian A. Drevon, Alicia Llorente, Knut Tomas Dalen, Marit Hjorth
Summary: Exercise-induced extracellular vesicles and their cargo miRNAs are associated with dysglycemia, obesity, and metabolic health. These findings provide a foundation for future studies on the exercise-induced effects of extracellular vesicles and their cargo miRNAs.
Article
Oncology
Ping Sun, Chao Wang, Ge Mang, Xiangli Xu, Shuai Fu, Jianfeng Chen, Xiaoqi Wang, Weiwei Wang, Hairu Li, Peng Zhao, Yifei Li, Qi Chen, Naixin Wang, Zhonghua Tong, Xin Fu, Ying Lang, Shasha Duan, Dongmei Liu, Maomao Zhang, Jiawei Tian
Summary: Mounting evidence suggests that extracellular vesicles (EVs) play an important role in cardiac physiology and pathology. This study found that EVs induced by acute myocardial infarction (AMI) can impair cardiomyocyte survival and worsen cardiac injury. The mechanism involves the EV-encapsulated miR-503 directly targeting PGC-1β and SIRT3, leading to mitochondrial metabolic dysfunction and cardiomyocyte death. Endothelial cells were identified as the primary source of miR-503 in EVs after AMI.
CLINICAL AND TRANSLATIONAL MEDICINE
(2022)
Article
Immunology
Pevindu Abeysinghe, Natalie Turner, Hassendrini Peiris, Kanchan Vaswani, Nick Cameron, Nathanael McGhee, Jayden Logan, Murray D. Mitchell
Summary: Ticks present in beef cattle worldwide result in significant economic losses, emphasizing the importance of evaluating and predicting tick resistance in global cattle trade. Utilizing miRNA profiles in exosomes as potential biomarkers for tick resistance is a promising approach, as miRNA have regulatory functions and differential expressions between groups. Identifying specific miRNA, such as MiR-449a, in high tick-resistant cattle could potentially serve as markers for improved resistance in breeding programs.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Immunology
Giuseppe Lia, Clara Di Vito, Stefania Bruno, Marta Tapparo, Lucia Brunello, Armando Santoro, Jacopo Mariotti, Stefania Bramanti, Elisa Zaghi, Michela Calvi, Lorenzo Comba, Martina Fasci, Luisa Giaccone, Giovanni Camussi, Eileen M. Boyle, Luca Castagna, Andrea Evangelista, Domenico Mavilio, Benedetto Bruno
Summary: Even with high-dose PT-Cy, acute and chronic GvHDs remain a clinical challenge. Reliable biomarkers for predicting their onset have yet to be identified. This study found a significant association between the expression profiles of EVs and their miRNA cargo with the onset of aGvHD. The correlation between EV expression profile and cargo with plasma biomarkers was also investigated.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Gastroenterology & Hepatology
Qichen Shen, Zhuizui Huang, Lingyan Ma, Jiachen Yao, Ting Luo, Yao Zhao, Yingping Xiao, Yuanxiang Jin
Summary: Inflammatory bowel disease (IBD) is a global disease without a cure, and disruption of the microbial ecosystem is considered a major cause. This study investigated the role of extracellular vesicles (EVs) and membrane vesicles (MVs) in host-microbe interactions in colitis. The researchers found that miR-181b-5p transplantation inhibited inflammation and promoted macrophage polarization, while miR-200b-3p regulated the microbiota composition and contributed to intestinal barrier integrity. MVs from feces and BMVs from colitis also had similar effects. These findings provide new insights and potential treatment strategies for colitis.
Article
Biochemistry & Molecular Biology
Anny Waloski Robert, Bruna Hilzendeger Marcon, Addeli Bez Batti Angulski, Sharon de Toledo Martins, Amanda Leitolis, Marco Augusto Stimamiglio, Alexandra Cristina Senegaglia, Alejandro Correa, Lysangela Ronalte Alves
Summary: This study investigates the effect of changing oxygen levels on the miRNA content of extracellular vesicles (EVs) derived from endothelial-like cells. The results show that the selective loading of miRNAs in the EVs is influenced by the oxygen status, and the targeted transcripts are involved in cell growth and survival pathways. These findings support the regenerative potential of EVs derived from endothelial-like cells and suggest that their properties can be fine-tuned by culture conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, Research & Experimental
Wei Yan, Li Song, Huihan Wang, Wei Yang, Liang Hu, Ying Yang
Summary: In this study, it was found that miRNA-181b-5p carried by extracellular vesicles in the peripheral blood of ALL patients can enter ALL cells and promote the malignancy of ALL cells by enhancing proliferation, migration, and invasion abilities.
JOURNAL OF TRANSLATIONAL MEDICINE
(2021)
Article
Physiology
Katie E. McGibbon, David B. Pyne, Laine E. Heidenreich, Robin Pla
Summary: The study found that adopting a relatively slower start strategy and an even pacing strategy in a 1500-m freestyle race can help swimmers achieve better results. In contrast, a fast start strategy did not perform well in terms of overall race time.
INTERNATIONAL JOURNAL OF SPORTS PHYSIOLOGY AND PERFORMANCE
(2021)
Article
Biochemistry & Molecular Biology
Serena Maggio, Barbara Canonico, Paola Ceccaroli, Emanuela Polidori, Andrea Cioccoloni, Luca Giacomelli, Carlo Ferri Marini, Giosue Annibalini, Marco Gervasi, Piero Benelli, Francesco Fabbri, Laura Del Coco, Francesco Paolo Fanizzi, Anna Maria Giudetti, Francesco Lucertini, Michele Guescini
Summary: Exercise-released extracellular vesicles (EVs) are a new class of exerkines that promote systemic beneficial effects. Differences in exercise protocols and the lack of standardized EV isolation protocols make it difficult to compare studies in the literature. This study investigated the release of EVs and associated miRNAs in response to different exercise regimens, finding increases in EVs and specific miRNAs following acute aerobic exercise (AAE). The results provide a better understanding of the role of circulating EVs in exercise-induced inflammatory processes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Virology
Steven Kopcho, Marina McDew-White, Wasifa Naushad, Mahesh Mohan, Chioma M. Okeoma
Summary: This study comprehensively analyzed the composition of circulating exmiRNAs and their association with extracellular particles, such as EVs and ECs, using sRNA sequencing. The study also found that SIV infection can alter the miRNA profile of EVs and suggested that miR-128-3p may be a potential target of HIV/SIV. This research has important implications for the development of biomarker approaches for various diseases based on the analysis of circulating exmiRNAs.
Article
Oncology
Jolene Chisholm, Sandor Haas-Neill, Peter Margetts, Khalid Al-Nedawi
Summary: Prostate cancer (PC) is a major cause of death in men, and current biomarker tests have limitations in accurately diagnosing the disease. This study examined the protein, mRNA, and miRNA content of extracellular vesicles (EVs) collected from PC patients and healthy subjects. The analysis revealed distinct groups of proteins, mRNAs, and miRNAs that differed between healthy subjects and PC patients, as well as between different tumor types. These findings suggest potential molecular markers for diagnosing PC.
FRONTIERS IN ONCOLOGY
(2022)
Article
Physiology
Suhong Park, Hyo Youl Moon
Summary: The study found that the number of urinary EVs significantly increased after exercise and returned to baseline levels after 1-hour rest. miRNA sequencing revealed alterations in four miRNAs pre- and post-exercise, suggesting that urinary EVs could potentially serve as a novel biomarker for acute exercise-induced fatigue.
EUROPEAN JOURNAL OF APPLIED PHYSIOLOGY
(2022)
Article
Cell Biology
Ekaterina Zubkova, Evgeniy Evtushenko, Irina Beloglazova, German Osmak, Phillip Koshkin, Alexander Moschenko, Mikhail Menshikov, Yelena Parfyonova
Summary: Research has shown that rat adipose-derived MSC-overexpressing stem cell factor (SCF) can induce endogenous regenerative processes and improve cardiac function. Genetic modification of MSC has a great impact on their miRNA composition. This study provides new insights into the regulatory networks underlying extracellular vesicle effects.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Ari Ogaki, Yuji Ikegaya, Ryuta Koyama
Summary: Extracellular vesicles (EVs) composed of lipid bilayer membranes and containing various molecules like mRNA and miRNA play a crucial role in intercellular communication in the brain. Astrocytes, as the most abundant glial cells in the mammalian brain, may be influenced by EVs from inside and outside the brain, subsequently affecting brain function. Further research on the specific targeting mechanism of EVs to astrocytes could open up new therapeutic possibilities for brain diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)