Biochemical and HDX Mass Spectral Characterization of the SARS-CoV-2 Nsp1 Protein

Structural insights into the activity regulation of full-length non-structural protein 1 from SARS-CoV-2

(2023) Ying Wang et al.   STRUCTURE

Drug targeting Nsp1-ribosomal complex shows antiviral activity against SARS-CoV-2

(2022) Mohammad Afsar et al.   eLife

A surprisingly simple three-state generic process for reversible protein denaturation by trifluoroethanol

(2022) Mujahid Hossain et al.   BIOPHYSICAL CHEMISTRY

Genomic monitoring of SARS-CoV-2 uncovers an Nsp1 deletion variant that modulates type I interferon response

(2021) Jing-wen Lin et al.   Cell Host & Microbe

1H, 13C, and 15N backbone chemical-shift assignments of SARS-CoV-2 non-structural protein 1 (leader protein)

(2021) Ying Wang et al.   Biomolecular NMR Assignments

SARS-CoV-2 uses a multipronged strategy to impede host protein synthesis

(2021) Yaara Finkel et al.   NATURE

Large-Scale Recombinant Production of the SARS-CoV-2 Proteome for High-Throughput and Structural Biology Applications

(2021) Nadide Altincekic et al.   Frontiers in Molecular Biosciences

Targeted in situ cross-linking mass spectrometry and integrative modeling reveal the architectures of three proteins from SARS-CoV-2

(2021) Moriya Slavin et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structural Basis and Function of the N Terminus of SARS-CoV-2 Nonstructural Protein 1

(2021) Kaitao Zhao et al.   Microbiology Spectrum

I(nsp1)ecting SARS-CoV-2–ribosome interactions

(2021) Matthieu Simeoni et al.   Communications Biology

1H, 13C and 15N resonance assignment of the SARS-CoV-2 full-length nsp1 protein and its mutants reveals its unique secondary structure features in solution

(2021) Tatiana Agback et al.   PLoS One

The N-terminal domain of SARS-CoV-2 nsp1 plays key roles in suppression of cellular gene expression and preservation of viral gene expression

(2021) Aaron S. Mendez et al.   Cell Reports

Coronavirus Nsp1: Immune Response Suppression and Protein Expression Inhibition

(2021) Shuai Yuan et al.   Frontiers in Microbiology

Emerging Mutations in Nsp1 of SARS-CoV-2 and Their Effect on the Structural Stability

(2021) Kejie Mou et al.   Pathogens

The Cap-Snatching SFTSV Endonuclease Domain Is an Antiviral Target

(2020) Wenjie Wang et al.   Cell Reports

The Novel Coronavirus SARS‐CoV‐2: From a Zoonotic Infection to Coronavirus Disease‐19 (COVID19)

(2020) Rafael dos Santos Bezerra et al.   JOURNAL OF MEDICAL VIROLOGY

Analysis of Temperature-Dependent H/D Exchange Mass Spectrometry Experiments

(2020) Nastaran N. Tajoddin et al.   ANALYTICAL CHEMISTRY

Structural basis for translational shutdown and immune evasion by the Nsp1 protein of SARS-CoV-2

(2020) Matthias Thoms et al.   SCIENCE

SARS-CoV-2 Nsp1 binds the ribosomal mRNA channel to inhibit translation

(2020) Katharina Schubert et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Characterization of accessory genes in coronavirus genomes

(2020) Christian Jean Michel et al.   Virology Journal

SARS-CoV-2 nsp1: Bioinformatics, Potential Structural and Functional Features, and Implications for Drug/Vaccine Designs

(2020) Yuan-Qin Min et al.   Frontiers in Microbiology

Structure of nonstructural protein 1 from SARS-CoV-2.

(2020) Lauren K. Clark et al.   JOURNAL OF VIROLOGY

Structural Characterization of SARS-CoV-2: Where We Are, and Where We Need to Be

(2020) Giuseppina Mariano et al.   Frontiers in Molecular Biosciences

Structural characterization of nonstructural protein 1 from SARS-CoV-2

(2020) Cameron Semper et al.   iScience

Reliable identification of significant differences in differential HX-MS measurements using a hybrid significance testing approach

(2019) Tyler S Hageman et al.   ANALYTICAL CHEMISTRY

The Influenza A Virus Endoribonuclease PA-X Usurps Host mRNA Processing Machinery to Limit Host Gene Expression

(2019) Lea Gaucherand et al.   Cell Reports

Recommendations for performing, interpreting and reporting hydrogen deuterium exchange mass spectrometry (HDX-MS) experiments

(2019) Glenn R. Masson et al.   NATURE METHODS

The entropic force generated by intrinsically disordered segments tunes protein function

(2018) Nicholas D. Keul et al.   NATURE

Middle East Respiratory Syndrome Coronavirus nsp1 Inhibits Host Gene Expression by Selectively Targeting mRNAs Transcribed in the Nucleus while Sparing mRNAs of Cytoplasmic Origin

(2015) Kumari G. Lokugamage et al.   JOURNAL OF VIROLOGY

Experimental Validation of the Role of Trifluoroethanol as a Nanocrowder

(2014) Robert M. Culik et al.   JOURNAL OF PHYSICAL CHEMISTRY B

Comprehensive analysis of loops at protein-protein interfaces for macrocycle design

(2014) Jason Gavenonis et al.   Nature Chemical Biology

Identification of Residues of SARS-CoV nsp1 That Differentially Affect Inhibition of Gene Expression and Antiviral Signaling

(2013) Andrew R. Jauregui et al.   PLoS One

Mapping of unfolding states of integral helical membrane proteins by GPS-NMR and scattering techniques: TFE-induced unfolding of KcsA in DDM surfactant

(2012) Antonello Calcutta et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

SARS Coronavirus nsp1 Protein Induces Template-Dependent Endonucleolytic Cleavage of mRNAs: Viral mRNAs Are Resistant to nsp1-Induced RNA Cleavage

(2011) Cheng Huang et al.   PLoS Pathogens

Trifluoroethanol-induced conformational transitions of proteins: Insights gained from the differences between α-lactalbumin and ribonuclease A

(2010) Klaus Gast et al.   PROTEIN SCIENCE

Severe Acute Respiratory Syndrome Coronavirus nsp1 Suppresses Host Gene Expression, Including That of Type I Interferon, in Infected Cells

(2008) K. Narayanan et al.   JOURNAL OF VIROLOGY