Online Collision-Induced Unfolding of Therapeutic Monoclonal Antibody Glyco-Variants through Direct Hyphenation of Cation Exchange Chromatography with Native Ion Mobility–Mass Spectrometry

Antibodies to watch in 2022

(2022) Hélène Kaplon et al.   mAbs

Studying protein structure and function by native separation–mass spectrometry

(2022) Guusje van Schaick et al.   Nature Reviews Chemistry

Multiattribute Monitoring of Antibody Charge Variants by Cation-Exchange Chromatography Coupled to Native Mass Spectrometry

(2021) Markus Haberger et al.   JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY

Simultaneous Monitoring of Monoclonal Antibody Variants by Strong Cation-Exchange Chromatography Hyphenated to Mass Spectrometry to Assess Quality Attributes of Rituximab-Based Biotherapeutics

(2021) Fiammetta Di Marco et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

State-of-the-Art Native Mass Spectrometry and Ion Mobility Methods to Monitor Homogeneous Site-Specific Antibody-Drug Conjugates Synthesis

(2021) Evolène Deslignière et al.   Pharmaceuticals

Towards a simple on-line coupling of ion exchange chromatography and native mass spectrometry for the detailed characterization of monoclonal antibodies

(2021) Amarande Murisier et al.   JOURNAL OF CHROMATOGRAPHY A

Analytical characterization of coformulated antibodies as combination therapy

(2020) Jun Kim et al.   mAbs

Middle level IM-MS and CIU experiments for improved therapeutic immunoglobulin subclass fingerprinting

(2020) Thomas Botzanowski et al.   ANALYTICAL CHEMISTRY

Toward Automation of Collision-Induced Unfolding Experiments through Online Size Exclusion Chromatography Coupled to Native Mass Spectrometry

(2020) Evolène Deslignière et al.   ANALYTICAL CHEMISTRY

CIUSuite 2: Next-Generation Software for the Analysis of Gas-Phase Protein Unfolding Data

(2019) Daniel A. Polasky et al.   ANALYTICAL CHEMISTRY

A Modified Drift Tube Ion Mobility-Mass Spectrometer for Charge-Multiplexed Collision-Induced Unfolding

(2019) Daniel D. Vallejo et al.   ANALYTICAL CHEMISTRY

Native Mass Spectrometry, Ion Mobility, and Collision-Induced Unfolding for Conformational Characterization of IgG4 Monoclonal Antibodies

(2018) Oscar Hernandez-Alba et al.   ANALYTICAL CHEMISTRY

Charge Variant Analysis of Monoclonal Antibodies Using Direct Coupled pH Gradient Cation Exchange Chromatography to High-Resolution Native Mass Spectrometry

(2018) Florian Füssl et al.   ANALYTICAL CHEMISTRY

Collision induced unfolding of isolated proteins in the gas phase: past, present, and future

(2018) Sugyan M Dixit et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Collision induced unfolding detects subtle differences in intact antibody glycoforms and associated fragments

(2018) Yuwei Tian et al.   INTERNATIONAL JOURNAL OF MASS SPECTROMETRY

ORIGAMI : A software suite for activated ion mobility mass spectrometry (aIM-MS) applied to multimeric protein assemblies

(2018) Lukasz G. Migas et al.   INTERNATIONAL JOURNAL OF MASS SPECTROMETRY

Ultrasensitive Characterization of Charge Heterogeneity of Therapeutic Monoclonal Antibodies Using Strong Cation Exchange Chromatography Coupled to Native Mass Spectrometry

(2018) Yuetian Yan et al.   ANALYTICAL CHEMISTRY

Charge variant native mass spectrometry benefits mass precision and dynamic range of monoclonal antibody intact mass analysis

(2018) Aaron O. Bailey et al.   mAbs

Influence of N-glycosylation on effector functions and thermal stability of glycoengineered IgG1 monoclonal antibody with homogeneous glycoforms

(2018) Ryuta Wada et al.   mAbs

Development of a fast workflow to screen the charge variants of therapeutic antibodies

(2017) Elsa Wagner-Rousset et al.   JOURNAL OF CHROMATOGRAPHY A

Determination of isoelectric points and relative charge variants of 23 therapeutic monoclonal antibodies

(2017) Alexandre Goyon et al.   JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES

Insights from native mass spectrometry approaches for top- and middle- level characterization of site-specific antibody-drug conjugates

(2017) Thomas Botzanowski et al.   mAbs

Next generation antibody drugs: pursuit of the 'high-hanging fruit'

(2017) Paul J. Carter et al.   NATURE REVIEWS DRUG DISCOVERY

Microheterogeneity of Recombinant Antibodies: Analytics and Functional Impact

(2017) Beate Beyer et al.   Biotechnology Journal

Evaluation of Ion Mobility-Mass Spectrometry for Comparative Analysis of Monoclonal Antibodies

(2016) Carly N. Ferguson et al.   JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY

Collision Induced Unfolding of Intact Antibodies: Rapid Characterization of Disulfide Bonding Patterns, Glycosylation, and Structures

(2015) Yuwei Tian et al.   ANALYTICAL CHEMISTRY

Ion-exchange chromatography for the characterization of biopharmaceuticals

(2015) Szabolcs Fekete et al.   JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS

Influence of glycosylation pattern on the molecular properties of monoclonal antibodies

(2014) Kai Zheng et al.   mAbs

In vitro and in vivo modifications of recombinant and human IgG antibodies

(2014) Hongcheng Liu et al.   mAbs

Improving pH gradient cation-exchange chromatography of monoclonal antibodies by controlling ionic strength

(2012) Liangyi Zhang et al.   JOURNAL OF CHROMATOGRAPHY A

The impact of glycosylation on monoclonal antibody conformation and stability

(2012) Kai Zheng et al.   mAbs

Collision Cross Sections of Proteins and Their Complexes: A Calibration Framework and Database for Gas-Phase Structural Biology

(2010) Matthew F. Bush et al.   ANALYTICAL CHEMISTRY

Post-translational Modifications Differentially Affect IgG1 Conformation and Receptor Binding

(2010) Damian Houde et al.   MOLECULAR & CELLULAR PROTEOMICS