Review
Cell Biology
Mabruka Alfaidi, Matthew L. Scott, Anthony Wayne Orr
Summary: The Nck family of adaptor proteins, Nck1 and Nck2, play crucial roles in linking phosphotyrosine signaling to changes in cytoskeletal dynamics and gene expression, affecting cellular phenotype. While they have redundant roles in vascular development and angiogenesis, recent studies suggest that Nck1 and Nck2 may differentially regulate cell phenotype in the adult vasculature. Domain-specific interactions likely mediate these isoform-selective effects, and could potentially be targeted for therapeutic interventions.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Briana C. Bywaters, Gonzalo M. Rivera
Summary: The non-catalytic region of tyrosine kinase (Nck) family of adaptors, consisting of Nck1 and Nck2, plays a key role in cellular information flow and specificity by recruiting components of signaling networks. Nck adaptors are involved in cytoskeletal remodeling, immune cell receptor activation, cell adhesion, and have some functional redundancy between Nck1 and Nck2.
JOURNAL OF CELL SCIENCE
(2021)
Article
Immunology
Pawel Borowicz, Vibeke Sundvold, Hanna Chan, Greger Abrahamsen, Hanna Kjelstrup, Tuula A. Nyman, Anne Spurkland
Summary: The study reveals that Lck Tyr(192) regulates Lck pTyr(394) and pTyr(505) activity, playing a crucial role in maintaining a pool of already active Lck in resting T cells. This provides an additional mechanism for fine-tuning Lck and T cell activity.
JOURNAL OF IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Zhengzheng Yan, Haihua Luo, Bingyao Xie, Tian Tian, Shan Li, Zhixia Chen, Jinghua Liu, Xuwen Zhao, Liyong Zhang, Yongqiang Deng, Timothy R. Billiar, Yong Jiang
Summary: SLP76 plays an important role in RAGE-mediated pro-inflammatory signaling, and targeting its signaling pathway could be a therapeutic approach for sepsis.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Rhiannon Morris, Yaoyuan Zhang, Julia Ellyard, Carola G. Vinuesa, James M. Murphy, Artem Laktyushin, Nadia J. Kershaw, Jeffrey J. Babon
Summary: The researchers presented structures of the substrate recognition (SH2) domain of LNK in complex with phosphorylated motifs from JAK2 and EPOR, providing insight into its binding specificity and mode of action.
NATURE COMMUNICATIONS
(2021)
Review
Immunology
Yael Dinur-Schejter, Irina Zaidman, Hagar Mor-Shaked, Polina Stepensky
Summary: Adaptor molecules play a crucial role in cell signaling by linking multiple proteins into intricate complexes, and mutations in these proteins have been associated with human inborn errors of immunity.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Zi Wang, Yubao Shan, Ru Wang, Heng Zhou, Rui Hu, Ying Li, Jiang Zhu, Yunhuang Yang, Maili Liu
Summary: This study investigated the interaction between SHIP2-SH2 and CagA protein from a structural perspective using NMR spectroscopy. The results showed that EPIYA-C and -D have high affinity to the binding site of SHIP2-SH2, while EPIYA-A and -B have weak affinity. The study also revealed the selective preference of SHIP2-SH2 for aliphatic residues at the pY+3 position in its ligand. These findings provide insights into the ligand sequence and the mechanism by which CagA protein hijacks SHIP2.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Keith P. Eidell, Alenka Lovy, Nicholas R. Sylvain, Frank A. Scangarello, Hayley Muendlein, Michael J. Ophir, Ken Nguyen, Maria-Cristina Seminario, Stephen C. Bunnell
Summary: Integrin engagement enhances T cell activation within the immune synapse. Myosin filaments surround and co-transport with SLP76 microclusters, with TCR ligand density governing their movement. Integrin beta 2, kindlin-3 and zyxin are required for complete centripetal transport, with LFA-1 and kindlin-3 having ligand-independent roles.
JOURNAL OF CELL SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Eduardo A. Gaspar-Morales, Anthony Waterston, Mourad Sadqi, Pedro Diaz-Parga, Ariell M. Smith, Arvind Gopinath, Roberto C. Andresen Eguiluz, Eva de Alba
Summary: The protein ASC can polymerize into intricate filament networks to assemble the inflammasome, and noncovalent, pH-responsive hydrogels can be created by controlling the pH. Natural variants of ASC and engineered proteins can also form hydrogels, demonstrating the capability of self-assembled protein hydrogels.
Article
Cell Biology
Jessica J. A. Hummel, Casper C. Hoogenraad
Summary: In this study, the authors systematically investigated the selectivity and regulation of KIF1A-driven transport of different types of vesicles, emphasizing the importance of phosphorylation for vesicle binding and the role of specific adaptors in achieving cargo specificity. They propose a model where motor dimerization, posttranslational modifications, and specific adaptors regulate selective KIF1A cargo trafficking.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Cell Biology
Alba Zuidema, Paul Atherton, Maaike Kreft, Liesbeth Hoekman, Onno B. Bleijerveld, Nagarjuna Nagaraj, Nanpeng Chen, Reinhard Fassler, Arnoud Sonnenberg
Summary: Integrins mediate cell adhesion and growth factor receptor signal transduction through interactions with PEAK1, Tensin3, and Shc1.
JOURNAL OF CELL BIOLOGY
(2022)
Review
Immunology
Chen Liu, Monika Raab, Yirui Gui, Christopher E. Rudd
Summary: T-cell activation is a complex process involving a network of kinases and adaptors that integrate surface signals with effector functions. SKAP1, a key immune-specific adaptor, plays multiple roles in regulating integrin activation, stop-signal, and cell cycling of T cells. Ongoing research on SKAP1 and its binding partners will provide insights into immune function regulation and potential new treatments for diseases.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Emanuele Bellacchio
Summary: The localization and activity of SHP2 tyrosine phosphatase are regulated by the binding of phosphotyrosine peptides to the tandem SH2 domains. The N-SH2 domain can inhibit the enzyme's activity by blocking the catalytic site, but this inhibition can be relieved by binding of phosphotyrosine peptides. A molecular dynamics simulation study reveals that the closed conformation of the self-inhibited phosphatase is stabilized by the interaction between the N-SH2 domain and a conserved peptide portion in PTPN11 exon 6.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Medicine, Research & Experimental
Yun Chen, Yunhui Gu, Xing Xiong, Yangyang Zheng, Xiao Liu, Weiqi Wang, Guoliang Meng
Summary: This review provides an overview of TRADD and discusses its roles in various diseases including infectious and inflammatory diseases, cardiovascular diseases, central nervous system diseases, cancer, endometriosis, hepatocyte proliferation, preterm birth, and perinatal development.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Review
Cell Biology
Szabolcs Sipeki, Kitti Koprivanacz, Tamas Takacs, Anita Kurilla, Loretta Laszlo, Virag Vas, Laszlo Buday
Summary: Signal transduction is essential for cells to perceive and respond to information, with subcellular hubs formed via protein interactions playing a central role. The SH2 and SH3 domains serve as prototypes for interaction domains that recognize proteins, lipids, nucleic acids, and small molecules. The lipid-binding properties of SH2 and SH3 domains are also important for modulating complex assembly.
Article
Cell Biology
Agnete Bratsberg Eriksen, Maria Lyngaas Torgersen, Kristine Lillebo Holm, Greger Abrahamsen, Anne Spurkland, Jan Oivind Moskaug, Anne Simonsen, Heidi Kiil Blomhoff
Article
Immunology
Monika Enqvist, Eivind Heggernes Ask, Elin Forslund, Mattias Carlsten, Greger Abrahamsen, Vivien Beziat, Sandra A Andersson, Marie Schaffer, Anne Spurkland, Yenan Bryceson, Bjorn Onfelt, Karl-Johan Malmberg
JOURNAL OF IMMUNOLOGY
(2015)
Article
Immunology
Agnete Bratsberg Eriksen, Tone Berge, Marte Wendel Gustavsen, Ingvild Sorum Leikfoss, Steffan Daniel Bos, Anne Spurkland, Hanne F. Harbo, Heidi Kill Blomhoff
JOURNAL OF NEUROIMMUNOLOGY
(2015)
Article
Genetics & Heredity
Loukas Moutsianas, Luke Jostins, Ashley H. Beecham, Alexander T. Dilthey, Dionysia K. Xifara, Maria Ban, Tejas S. Shah, Nikolaos A. Patsopoulos, Lars Alfredsson, Carl A. Anderson, Katherine E. Attfield, Sergio E. Baranzini, Jeffrey Barrett, Thomas M. C. Binder, David Booth, Dorothea Buck, Elisabeth G. Celius, Chris Cotsapas, Sandra D'Alfonso, Calliope A. Dendrou, Peter Donnelly, Benedicte Dubois, Bertrand Fontaine, Lars Fugger, An Goris, Pierre-Antoine Gourraud, Christiane Graetz, Bernhard Hemmer, Jan Hillert, Ingrid Kockum, Stephen Leslie, Christina M. Lill, Filippo Martinelli-Boneschi, Jorge R. Oksenberg, Tomas Olsson, Annette Oturai, Janna Saarela, Helle Bach Sondergaard, Anne Spurkland, Bruce Taylor, Juliane Winkelmann, Frauke Zipp, Jonathan L. Haines, Margaret A. Pericak-Vance, Chris C. A. Spencer, Graeme Stewart, David A. Hafler, Adrian J. Ivinson, Hanne F. Harbo, Stephen L. Hauser, Philip L. De Jager, Alastair Compston, Jacob L. McCauley, Stephen Sawcer, Gil McVean
Article
Multidisciplinary Sciences
P. Moussa, G. Abrahamsen, N. Fodil, R. P. Gopalakrishnan, M. Mancini, E. Dissen, P. C. Saether, S. A. Wiltshire, G. A. Boivin, G. Caignard, A. Spurkland, S. M. Vidal
SCIENTIFIC REPORTS
(2015)
Article
Genetics & Heredity
T. Berge, I. S. Leikfoss, I. S. Brorson, S. D. Bos, C. M. Page, M. W. Gustavsen, A. Bjolgerud, T. Holmoy, E. G. Celius, J. Damoiseaux, J. Smolders, H. F. Harbo, A. Spurkland
GENES AND IMMUNITY
(2016)
Article
Biochemical Research Methods
Meseret Habtamu, Markos Abebe, Abraham Aseffa, Anne Margarita Dyrhol-Riise, Anne Spurkland, Greger Abrahamsen
JOURNAL OF IMMUNOLOGICAL METHODS
(2018)
Article
Biotechnology & Applied Microbiology
Thorny Cecilie Bie Andersen, Kjersti Lindsjo, Cecilie Dahl Hem, Lise Koll, Per Eugen Kristiansen, Lars Skjeldal, Amy H. Andreotti, Anne Spurkland
Article
Multidisciplinary Sciences
Ingvild S. Leikfoss, Pankaj K. Keshari, Marte W. Gustavsen, Anja Bjolgerud, Ina S. Brorson, Elisabeth G. Celius, Anne Spurkland, Steffan D. Bos, Hanne F. Harbo, Tone Berge
Article
Immunology
A. D. Pandya, T. B. Leergaard, E. Dissen, G. Haraldsen, A. Spurkland
SCANDINAVIAN JOURNAL OF IMMUNOLOGY
(2014)
Article
Immunology
Pawel Borowicz, Hanna Chan, Daniel Medina, Simon Gumpelmair, Hanna Kjelstrup, Anne Spurkland
SCANDINAVIAN JOURNAL OF IMMUNOLOGY
(2020)
Review
Immunology
Pawel Borowicz, Hanna Chan, Anette Hauge, Anne Spurkland
SCANDINAVIAN JOURNAL OF IMMUNOLOGY
(2020)
Article
Immunology
Pawel Borowicz, Vibeke Sundvold, Hanna Chan, Greger Abrahamsen, Hanna Kjelstrup, Tuula A. Nyman, Anne Spurkland
Summary: The study reveals that Lck Tyr(192) regulates Lck pTyr(394) and pTyr(505) activity, playing a crucial role in maintaining a pool of already active Lck in resting T cells. This provides an additional mechanism for fine-tuning Lck and T cell activity.
JOURNAL OF IMMUNOLOGY
(2021)
Meeting Abstract
Clinical Neurology
T. Berge, I. S. Leikfoss, I. S. Brorson, S. D. Bos, C. M. Page, M. W. Gustavsen, A. Bjolgerud, T. Holmoy, E. G. Celius, J. Damoiseaux, J. Smolders, H. F. Harbo, A. Spurkland
MULTIPLE SCLEROSIS JOURNAL
(2015)