Genetically programmed cell-based synthesis of non-natural peptide and depsipeptide macrocycles

Creating custom synthetic genomes in Escherichia coli with REXER and GENESIS

(2021) Wesley E. Robertson et al.   Nature Protocols

Sense codon reassignment enables viral resistance and encoded polymer synthesis

(2021) Wesley E. Robertson et al.   SCIENCE

Rapid discovery and evolution of orthogonal aminoacyl-tRNA synthetase–tRNA pairs

(2020) Daniele Cervettini et al.   NATURE BIOTECHNOLOGY

Engineered triply orthogonal pyrrolysyl–tRNA synthetase/tRNA pairs enable the genetic encoding of three distinct non-canonical amino acids

(2020) Daniel L. Dunkelmann et al.   Nature Chemistry

Total synthesis of Escherichia coli with a recoded genome

(2019) Julius Fredens et al.   NATURE

Modification and de novo design of non-ribosomal peptide synthetases using specific assembly points within condensation domains

(2019) Kenan A. J. Bozhüyük et al.   Nature Chemistry

Engineering an Automaturing Transglutaminase with Enhanced Thermostability by Genetic Code Expansion with Two Codon Reassignments

(2018) Kazumasa Ohtake et al.   ACS Synthetic Biology

Trapping biosynthetic acyl-enzyme intermediates with encoded 2,3-diaminopropionic acid

(2018) Nicolas Huguenin-Dezot et al.   NATURE

Controlling orthogonal ribosome subunit interactions enables evolution of new function

(2018) Wolfgang H. Schmied et al.   NATURE

Recombinant Macrocyclic Lanthipeptides Incorporating Non-Canonical Amino Acids

(2017) Claudio Zambaldo et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Nonribosomal biosynthesis of backbone-modified peptides

(2017) David L. Niquille et al.   Nature Chemistry

Ribosome-mediated synthesis of natural product-like peptides via cell-free translation

(2016) Rumit Maini et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Facile Removal of Leader Peptides from Lanthipeptides by Incorporation of a Hydroxy Acid

(2015) Noah A. Bindman et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Rationally evolving tRNAPylfor efficient incorporation of noncanonical amino acids

(2015) Chenguang Fan et al.   NUCLEIC ACIDS RESEARCH

Structural Insights into Incorporation of Norbornene Amino Acids for Click Modification of Proteins

(2013) Sabine Schneider et al.   CHEMBIOCHEM

A Cyclic Peptide Inhibitor of HIF-1 Heterodimerization That Inhibits Hypoxia Signaling in Cancer Cells

(2013) Elena Miranda et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Ligation of Expressed Protein α-Hydrazides via Genetic Incorporation of an α-Hydroxy Acid

(2012) Yi-Ming Li et al.   ACS Chemical Biology

N-Acetyl lysyl-tRNA synthetases evolved by a CcdB-based selection possessN-acetyl lysine specificity in vitro and in vivo

(2012) Takuya Umehara et al.   FEBS LETTERS

A Rationally Designed Pyrrolysyl-tRNA Synthetase Mutant with a Broad Substrate Spectrum

(2012) Yane-Shih Wang et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Evolution of cyclic peptide protease inhibitors

(2011) T. S. Young et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Polymerization of α-Hydroxy Acids by Ribosomes

(2008) Atsushi Ohta et al.   CHEMBIOCHEM

Multistep Engineering of Pyrrolysyl-tRNA Synthetase to Genetically Encode Nɛ-(o-Azidobenzyloxycarbonyl) lysine for Site-Specific Protein Modification

(2008) Tatsuo Yanagisawa et al.   CHEMISTRY & BIOLOGY

Recognition of Non-α-amino Substrates by Pyrrolysyl-tRNA Synthetase

(2008) Takatsugu Kobayashi et al.   JOURNAL OF MOLECULAR BIOLOGY

Addition of an α-Hydroxy Acid to the Genetic Code of Bacteria

(2007) Jiantao Guo et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION