Engineered triply orthogonal pyrrolysyl–tRNA synthetase/tRNA pairs enable the genetic encoding of three distinct non-canonical amino acids

Mutually orthogonal nonsense-suppression systems and conjugation chemistries for precise protein labeling at up to three distinct sites

(2019) James S Italia et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Total synthesis of Escherichia coli with a recoded genome

(2019) Julius Fredens et al.   NATURE

Mutually orthogonal pyrrolysyl-tRNA synthetase/tRNA pairs

(2018) Julian C. W. Willis et al.   Nature Chemistry

HMMER web server: 2018 update

(2018) Simon C Potter et al.   NUCLEIC ACIDS RESEARCH

Methanomethylophilus alvus Mx1201 provides basis for mutual orthogonal pyrrolysyl tRNA/aminoacyl-tRNA synthetase pairs in mammalian cells.

(2018) Birthe Meineke et al.   ACS Chemical Biology

An Evolved Methanomethylophilus alvus Pyrrolysyl-tRNA Synthetase/tRNA Pair Is Highly Active and Orthogonal in Mammalian Cells

(2018) Václav Beránek et al.   BIOCHEMISTRY

Controlling orthogonal ribosome subunit interactions enables evolution of new function

(2018) Wolfgang H. Schmied et al.   NATURE

UniProt: a worldwide hub of protein knowledge

(2018)   NUCLEIC ACIDS RESEARCH

Expanding and reprogramming the genetic code

(2017) Jason W. Chin   NATURE

A semi-synthetic organism that stores and retrieves increased genetic information

(2017) Yorke Zhang et al.   NATURE

Labeling and identifying cell-specific proteomes in the mouse brain

(2017) Toke P Krogager et al.   NATURE BIOTECHNOLOGY

Crystal structures reveal an elusive functional domain of pyrrolysyl-tRNA synthetase

(2017) Tateki Suzuki et al.   Nature Chemical Biology

Biosynthesis and genetic encoding of phosphothreonine through parallel selection and deep sequencing

(2017) Michael Shaofei Zhang et al.   NATURE METHODS

Precise quantification of translation inhibition by mRNA structures that overlap with the ribosomal footprint in N-terminal coding sequences

(2017) Amin Espah Borujeni et al.   NUCLEIC ACIDS RESEARCH

In Vivo Biosynthesis of a β-Amino Acid-Containing Protein

(2016) Clarissa Melo Czekster et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Translation Initiation is Controlled by RNA Folding Kinetics via a Ribosome Drafting Mechanism

(2016) Amin Espah Borujeni et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Defining synonymous codon compression schemes by genome recoding

(2016) Kaihang Wang et al.   NATURE

The EcoCyc database: reflecting new knowledge aboutEscherichia coliK-12

(2016) Ingrid M. Keseler et al.   NUCLEIC ACIDS RESEARCH

Efficient genetic encoding of phosphoserine and its nonhydrolyzable analog

(2015) Daniel T Rogerson et al.   Nature Chemical Biology

Expanding and Reprogramming the Genetic Code of Cells and Animals

(2014) Jason W. Chin   Annual Review of Biochemistry

Comparative genomics highlights the unique biology of Methanomassiliicoccales, a Thermoplasmatales-related seventh order of methanogenic archaea that encodes pyrrolysine

(2014) Guillaume Borrel et al.   BMC GENOMICS

Proteome labeling and protein identification in specific tissues and at specific developmental stages in an animal

(2014) Thomas S Elliott et al.   NATURE BIOTECHNOLOGY

Optimized orthogonal translation of unnatural amino acids enables spontaneous protein double-labelling and FRET

(2014) Kaihang Wang et al.   Nature Chemistry

Unique Characteristics of the Pyrrolysine System in the 7th Order of Methanogens: Implications for the Evolution of a Genetic Code Expansion Cassette

(2014) Guillaume Borrel et al.   ARCHAEA-AN INTERNATIONAL MICROBIOLOGICAL JOURNAL

A Tryptophanyl-tRNA Synthetase/tRNA Pair for Unnatural Amino Acid Mutagenesis inE. coli

(2013) Abhishek Chatterjee et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

A Versatile Platform for Single- and Multiple-Unnatural Amino Acid Mutagenesis in Escherichia coli

(2013) Abhishek Chatterjee et al.   BIOCHEMISTRY

A simple system for expression of proteins containing 3-azidotyrosine at a pre-determined site in Escherichia coli

(2013) Akiyoshi Ikeda-Boku et al.   JOURNAL OF BIOCHEMISTRY

RNAstructure: web servers for RNA secondary structure prediction and analysis

(2013) Stanislav Bellaousov et al.   NUCLEIC ACIDS RESEARCH

Translation rate is controlled by coupled trade-offs between site accessibility, selective RNA unfolding and sliding at upstream standby sites

(2013) Amin Espah Borujeni et al.   NUCLEIC ACIDS RESEARCH

PylSn and the Homologous N-terminal Domain of Pyrrolysyl-tRNA Synthetase Bind the tRNA That Is Essential for the Genetic Encoding of Pyrrolysine

(2012) Ruisheng Jiang et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Evolution of multiple, mutually orthogonal prolyl-tRNA synthetase/tRNA pairs for unnatural amino acid mutagenesis in Escherichia coli

(2012) A. Chatterjee et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Reprogramming the Genetic Code

(2012) J. W. Chin   SCIENCE

HMMER web server: interactive sequence similarity searching

(2011) R. D. Finn et al.   NUCLEIC ACIDS RESEARCH

Expanding the Genetic Code of Escherichia coli with Phosphoserine

(2011) H.-S. Park et al.   SCIENCE

De NovoGeneration of Mutually Orthogonal Aminoacyl-tRNA Synthetase/tRNA Pairs

(2010) Heinz Neumann et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Encoding multiple unnatural amino acids via evolution of a quadruplet-decoding ribosome

(2010) Heinz Neumann et al.   NATURE

Rational design of an orthogonal tryptophanyl nonsense suppressor tRNA

(2010) Randall A. Hughes et al.   NUCLEIC ACIDS RESEARCH

Pyrrolysyl-tRNA synthetase–tRNAPyl structure reveals the molecular basis of orthogonality

(2009) Kayo Nozawa et al.   NATURE

Automated design of synthetic ribosome binding sites to control protein expression

(2009) Howard M Salis et al.   NATURE BIOTECHNOLOGY

Multistep Engineering of Pyrrolysyl-tRNA Synthetase to Genetically Encode Nɛ-(o-Azidobenzyloxycarbonyl) lysine for Site-Specific Protein Modification

(2008) Tatsuo Yanagisawa et al.   CHEMISTRY & BIOLOGY

Genetically encoding Nε-acetyllysine in recombinant proteins

(2008) Heinz Neumann et al.   Nature Chemical Biology