Article
Biochemistry & Molecular Biology
Yuko Ibuki, Yukako Komaki, Guang Yang, Tatsushi Toyooka
Summary: This study found that UVA1 can enhance UVB-induced cell death and trigger the formation of DNA double-strand breaks. This suggests that simultaneous exposure to high doses of UVA1 and UVB may pose potential health risks.
PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Arun Mouli Kolinjivadi, Haresh Sankar, Ramveer Choudhary, Lavina Sierra Tay, Tuan Zea Tan, Naoko Murata-Kamiya, Dominic Chih-Cheng Voon, Dennis Kappei, Masanori Hatakeyama, Vaidehi Krishnan, Yoshiaki Ito
Summary: The bacterial pathogen H. pylori exploits the host DNA repair proteins to induce genomic instability by downregulating key DNA repair genes such as FANCI, FANCD2, BRCA1, and BRCA2 through the virulence factor CagA. This results in impaired fork stability, increased DNA double strand breaks, and compromised genome stability, revealing a novel mechanism of bacterial virulence.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell & Tissue Engineering
Marziyeh Tolouee, Koen D. W. Hendriks, Fia Fia Lie, Lucas P. Gartzke, Maaike Goris, Femke Hoogstra-Berends, Steven Bergink, Robert H. Henning
Summary: Cooling at 4 degrees C can cause extensive DNA damage, including double-strand breaks, in cells. This damage is time- and temperature-dependent and is associated with an excess production of reactive oxygen species (ROS). Subsequent rewarming fails to fully repair the DNA damage. Precluding ROS formation can reduce the DNA damage. Cooling-induced DNA damage may significantly affect cell survival, proliferation, and genomic stability, with relevance in cellular and organ function.
CELL TRANSPLANTATION
(2022)
Article
Biochemistry & Molecular Biology
Alexandr Zalessky, Yuriy Fedotov, Elizaveta Yashkina, Viktor Nadtochenko, Andreyan N. Osipov
Summary: The study found that different pulse power densities of femtosecond laser radiation can lead to the formation of XRCC1 and gamma H2AX protein foci, indicating that femtosecond laser radiation can be considered as a tool for local ionization of biological material.
Article
Environmental Sciences
Yanfeng Sun, Yanjian Wan, Ying Jiang, Huaiji Wang
Summary: The study found that exposure to Acetaminophen (AAP) was common in young children in central and south China, with AAP concentrations positively correlated with oxidative stress biomarkers 8-hydroxy-guanosine (8-OHG) and 8-hydroxy-2'-deoxyguanosine (8-OHdG). This suggests a potential association between AAP exposure and DNA and RNA damage.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Biology
Galina A. Posypanova, Marya G. Ratushnyak, Yuliya P. Semochkina, Alexander N. Strepetov
Summary: The study aimed to investigate the effects of gamma-neutron radiation on mouse neural stem/progenitor cells. The research found that gamma-neutron radiation can cause cell death and proliferation, with a high relative biological efficiency. Special measures are needed to protect the neurogenic regions of the brain.
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY
(2022)
Article
Biology
Sonia Spandole-Dinu, Ana-Maria Catrina, Oana Cristina Voinea, Alina Andone, Speranta Radu, Cerasela Haidoiu, Octavian Calborean, Radu Gabriel Hertzog, Diana Mihaela Popescu
Summary: This pilot study aimed to evaluate the potential radioprotective effect of Natural SOD (R) in Wistar rats exposed to ionizing radiation. The results showed a potential protective effect of Natural SOD (R) against radiation-induced DNA damage, particularly in the testes. Further investigations on the long-term effects of X-ray in animals administered Natural SOD (R) are needed for a better estimation of the radioprotective effect.
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Sunwoo Min, Ho-Soo Lee, Jae-Hoon Ji, Yungyeong Heo, Yonghyeon Kim, Sunyoung Chae, Yong Won Choi, Ho-Chul Kang, Makoto Nakanishi, Hyeseong Cho
Summary: This study demonstrates that histone H2A acetylation at K118 is enriched in transcriptionally active regions, and the RSF1-HDAC1 complex is involved in deacetylating H2A(X)-K118 under DNA damage conditions, affecting DNA repair and transcriptional repression.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Jieming Jiang, Xiaolin Ou, Danlu Han, Zhipeng He, Song Liu, Ning Mao, Zhonghui Zhang, Chang-Lian Peng, Jianbin Lai, Chengwei Yang
Summary: This study reveals that localization of the SMC5/6 complex at double-strand breaks in plant cells is dependent on the protein scaffold consisting of IDN2, CDC5, and ADA2b, and this recruitment is further mediated by DNA damage-induced RNAs.
Article
Multidisciplinary Sciences
Kelsey Gallant, Arsun Bektas, Mary Kaileh, Ana Lustig, Luigi Ferrucci, Gianluca Pegoraro, Tom Misteli
Summary: DNA damage is a prominent biomarker for various diseases and the aging process. Traditional microscopy or cytometric methods are not ideal for large-scale studies. We have developed a high-throughput single-cell assay that accurately measures DNA damage using two major markers. The assay demonstrates sensitivity and low variability in various human immune cells, providing a novel approach for large-scale evaluation of DNA damage.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Physical
Leo Sala, Hlib Lyshchuk, Jana Sachova, David Chvatil, Jaroslav Kocisek
Summary: DNA origami nanoframes were used to study the effect of nucleoside substitutions on radiation-induced DNA damage. The results showed that increased AT or GC content did not increase the number of DNA strand breaks. Different nucleoside analogues had different effects on DSB, indicating different radiosensitization mechanisms. Bystander effects were also observed when substitutions were introduced in the parallel DNA sequence. This new approach overcomes limitations of previous methods and enables detailed studies of poorly understood conformation or bystander effects in radiation-induced DNA damage.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2022)
Review
Oncology
Davide Valente, Maria Pia Gentileschi, Antonino Guerrisi, Vicente Bruzzaniti, Aldo Morrone, Silvia Soddu, Alessandra Verdina
Summary: This article discusses the induction of gamma H2AX in individuals exposed to ionizing radiation and its applications in clinical studies. By analyzing the various conditions that induce H2AX phosphorylation and describing commonly used methods for gamma H2AX determination, the assessment of DSBs following IR exposure can be optimized.
Article
Acoustics
Cecile Fant, Adeline Granzotto, Jean-Louis Mestas, Jacqueline Ngo, Maxime Lafond, Cyril Lafon, Nicolas Foray, Frederic Padilla
Summary: The combination of unseeded stable cavitation and doxorubicin was shown to increase DNA double-strand break induction, reduce cell viability, and proliferation significantly. Mechanistic study suggested that the presence of cavitation played a vital role in enhancing early DNA double-strand break induction, likely mediated by a bystander effect with the release of extracellular calcium.
ULTRASOUND IN MEDICINE AND BIOLOGY
(2021)
Article
Engineering, Biomedical
Jung-Soo Suh, Tae-Jin Kim
Summary: Understanding the spatiotemporal behavior of DNA double-strand breaks (DSBs) is crucial for studying DNA damage and repair processes. Traditional methods rely on biochemical assays, such as immunostaining, to detect DSBs. However, a reliable technique for real-time visualization and assessment of DSB activity in living cells is still lacking. In this study, we developed a novel DNA biosensor based on fluorescence resonance energy transfer (FRET), which specifically reacts to gamma H2AX activity induced by drugs or ionizing radiation (IR). Our biosensor enables the quantification of DSB events at high spatiotemporal resolutions, providing a new experimental tool for studying DNA double-strand breaks. This biosensor has the potential to elucidate the molecular mechanisms underlying DNA damage and repair processes.
BIOMATERIALS RESEARCH
(2023)
Article
Oncology
Thorsten Derlin, Natalia Bogdanova, Fiona Ohlendorf, Dhanya Ramachandran, Rudolf A. Werner, Tobias L. Ross, Hans Christiansen, Frank M. Bengel, Christoph Henkenberens
Summary: Assessment of gamma-H2AX and 53BP1 foci formation in peripheral blood lymphocytes of patients receiving radioligand therapy may hold promise for predicting early treatment response and subclinical hematotoxicity.
Review
Genetics & Heredity
Bryant C. Nelson, Christa W. Wright, Yuko Ibuki, Maria Moreno-Villanueva, Hanna L. Karlsson, Giel Hendriks, Christopher M. Sims, Neenu Singh, Shareen H. Doak
Article
Toxicology
Tatsushi Toyooka, Yukie Yanagiba, Megumi Suda, Yuko Ibuki, Rui-Sheng Wang
TOXICOLOGY LETTERS
(2017)
Article
Chemistry, Medicinal
Guang Yang, Yuko Ibuki
CHEMICAL RESEARCH IN TOXICOLOGY
(2018)
Article
Chemistry, Medicinal
Kazutaka Hirakawa, Dongyan Ouyang, Yuko Ibuki, Shiho Hirohara, Shigetoshi Okazaki, Eiji Kono, Naohiro Kanayama, Jotaro Nakazaki, Hiroshi Segawa
CHEMICAL RESEARCH IN TOXICOLOGY
(2018)
Article
Toxicology
Tatsushi Toyooka, Yukie Yanagiba, Yuko Ibuki, Rui-Sheng Wang
JOURNAL OF APPLIED TOXICOLOGY
(2018)
Article
Biotechnology & Applied Microbiology
Guang Yang, Yukako Komaki, Yuko Ibuki
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS
(2018)
Article
Pharmacology & Pharmacy
Kazuhiro Nishiyama, Takuro Numaga-Tomita, Yasuyuki Fujimoto, Tomohiro Tanaka, Chiemi Toyama, Akiyuki Nishimura, Tomohiro Yamashita, Naoya Matsunaga, Satoru Koyanagi, Yasu-Taka Azuma, Yuko Ibuki, Koji Uchida, Shigehiro Ohdo, Motohiro Nishida
BRITISH JOURNAL OF PHARMACOLOGY
(2019)
Article
Chemistry, Medicinal
Kazutaka Hirakawa, Ayaka Suzuki, Dongyan Ouyang, Shigetoshi Okazaki, Yuko Ibuki, Jotaro Nakazaki, Hiroshi Segawa
CHEMICAL RESEARCH IN TOXICOLOGY
(2019)
Article
Toxicology
Guang Yang, Yukako Komaki, Ikuma Yoshida, Yuko Ibuki
TOXICOLOGY IN VITRO
(2019)
Article
Biochemistry & Molecular Biology
Yuko Ibuki, Yukako Komaki, Guang Yang, Tatsushi Toyooka
Summary: This study found that UVA1 can enhance UVB-induced cell death and trigger the formation of DNA double-strand breaks. This suggests that simultaneous exposure to high doses of UVA1 and UVB may pose potential health risks.
PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Tetsuya Fukuda, Yukako Komaki, Yuta Mori, Yuko Ibuki
Summary: This study revealed that low extracellular pH inhibits the nucleotide excision repair (NER) pathway, potentially contributing to carcinogenesis in inflamed tissues with acidic pH.
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS
(2021)
Article
Chemistry, Medicinal
Mariko Shikata, Tatsushi Toyooka, Yukako Komaki, Yuko Ibuki
Summary: The study found that NNK globally acetylated histone H3, impacting gamma-H2AX generation. Activation of the nicotinic acetylcholine receptor alpha 7nAChR by NNK activated the PI3K/Akt pathway, leading to Ac-H3. The enhanced Ac-H3 contributed to increased gamma-H2AX, implying that NNK-induced DNA damage is influenced by the alpha 7nAChR-mediated signal transduction pathway.
CHEMICAL RESEARCH IN TOXICOLOGY
(2021)
Article
Chemistry, Medicinal
Kazutaka Hirakawa, Naoki Kishimoto, Yoshinobu Nishimura, Yuko Ibuki, Masaaki Fuki, Shigetoshi Okazaki
Summary: The photoexcited state of the trimer is almost the same as that of the monomer, and these phosphorus(V)porphyrins demonstrate a similar protein-photodamaging activity. The difference in association between the photosensitizer molecules and cells is the key factor of phototoxicity by these phosphorus(V)porphyrins.
CHEMICAL RESEARCH IN TOXICOLOGY
(2023)
Article
Oncology
Guang Yang, Yuko Ibuki
Article
Genetics & Heredity
Tatsuya Kato, Tatsushi Toyooka, Yuko Ibuki, Shuichi Masuda, Masatoshi Watanabe, Yukari Totsuka
GENES AND ENVIRONMENT
(2017)
Article
Biochemistry & Molecular Biology
Girgis Obaid, Menitte Eroy, Jie Zhao, Shazia Bano, Mari Mino-Kenudson, Tayyaba Hasan
Summary: The presence of desmoplasia in pancreatic ductal adenocarcinoma (PDAC) is associated with poor treatment outcomes and reduced drug delivery. By analyzing collagen subtypes in PDAC tumors following different treatment regimens, immunofluorescence profiling can predict long-term survival and tumor growth inhibition. This information can aid in personalized treatment and improve patient survival.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Fatemeh Malekpour, Rashin Bahrami, Mahshid Hodjat, Neda Hakimiha, Behnam Bolhari, Aidin Sooratgar, Soheil Niavarzi
Summary: This study investigated the effect of photobiomodulation (PBM) therapy using an 808 nm diode laser on cellular modulation mechanisms in regenerative endodontic procedures (REPs). The results showed that PBM treatment significantly increased cell viability and migration, and enhanced the release of TGF-131 from dentin.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Pranathi Tata, Ramakrishnan Ganesan, Jayati Ray Dutta
Summary: Thin film coatings with potent antibacterial properties are widely used in medical devices, frequently touched surfaces, and food packaging. However, achieving antibacterial performance under both light and dark conditions remains a challenge. In this study, we successfully prepared AgBr-coated ZnO nanorod thin film architecture using a unique surfactant-mediated solution-phase spin-coating approach. The resulting structures showed dual functionality as antimicrobial agents and photosensitizers, effectively enhancing the visible-light photodisinfection efficacy of ZnO nanorods. The concentration of the surfactant-based precursor solution could be modulated to control the AgBr content. Additionally, components like urea and lactic acid were found to enhance the photocatalytic efficiency of the structures.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
(2024)
