Article
Engineering, Environmental
Teng Zhang, Jiashun Cao, Qirong Zhu, Boming Fu, E. Yang, Fang Fang, Qian Feng, Jingyang Luo
Summary: The study demonstrates that glycerol-driven partial denitrification reactor can form granular sludge with excellent settleability and high microbial activity, achieving stable and efficient nitrite accumulation. The mature granules are compact in structure, dominated by cocci, and the proteins in extracellular polymeric substances play a key role in the granulation process.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Agricultural Engineering
Zhe Liu, Jingwen Wang, Shumin Zhang, Yiwen Hou, Jiaxuan Wang, Min Gao, Xingdu Chen, Aining Zhang, Yongjun Liu, Zhihua Li
Summary: The formation characteristics of algal-bacteria granular sludge (ABGS) under different light intensities were investigated. It was found that stronger light favored the improvement of sludge characteristics and nutrient removal performances during the growing stage, while weaker light ensured stable operation of the system during the mature stage. The dominant bacterial genus in mature ABGS cultured under low light intensity was Zoogloe, and different algal genus were dominant.
BIORESOURCE TECHNOLOGY
(2023)
Article
Engineering, Environmental
Lifang Yu, Qiong Zhang, Ren Li, Bingchuang Qiao, Ze Wang, Lanxiang Zheng, Dangcong Peng
Summary: The migration of microbial communities in a synergistic partial denitrification/anammox system was investigated. The anammox process was the dominant nitrogen removal pathway, with an average contribution of 74.31%. Nitrate-reducing bacteria tended to immigrate to anammox biofilms, possibly due to different extracellular polymeric substance (EPS)-mediated mechanisms.
Article
Green & Sustainable Science & Technology
Wanqing Ding, Wenbiao Jin, Xu Zhou, Qinhui Yang, Chuan Chen, Qilin Wang
Summary: This study investigated the dewaterability of anaerobic granular sludge (AGS) by Fe(II)-PDS conditioning, finding that Fe(II)-PDS conditioning can enhance dewaterability by breaking down EPS layers and microbial cell structures, releasing bound water and facilitating sludge re-flocculation. Protein was identified as the primary component in AGS and showed a strong correlation with dewaterability.
JOURNAL OF CLEANER PRODUCTION
(2021)
Article
Engineering, Environmental
Ziang Su, Junting Zhao, Zheng Lu, Mengdan Wang, Congcong Guo, Xue Song, Xuebai Guo, Ming Cai, Zhenjun Wu
Summary: This study investigated the effects of different temperature conditions on nitritation denitrification. The results showed that stepwise cooling facilitated a faster start-up of the process and achieved higher nitrite accumulation compared to continuous low temperature conditions. Additionally, lower temperatures significantly inhibited denitrification activity.
JOURNAL OF WATER PROCESS ENGINEERING
(2022)
Article
Green & Sustainable Science & Technology
Yabing Xiao, Yao Shen, Bin Ji
Summary: This research focused on cultivating MBGS from activated sludge by inoculation. The results showed that inoculation helped in the successful formation of MBGS within a short period of time, and achieved stable removal of organics, ammonia, and phosphorus. Functional microbial communities, including phosphorus accumulating organisms, were enhanced by the inoculation.
JOURNAL OF CLEANER PRODUCTION
(2022)
Article
Engineering, Environmental
Benedetta Pagliaccia, Emiliano Carretti, Mirko Severi, Debora Berti, Claudio Lubello, Tommaso Lotti
Summary: The recovery and conversion of Extracellular Polymeric Substances (EPS) from sewage sludge into bio-based commodities could improve the economics and environmental sustainability of wastewater treatment. The application of EPS as a biosorbent for heavy metal removal showed high adsorption capacities and diverse adsorption mechanisms, making it suitable for treating complex wastewaters.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Environmental Sciences
Zhe Liu, Rushuo Yang, Zhengyang Li, Fangzhi Ning, Jiaxuan Wang, Min Gao, Aining Zhang, Yongjun Liu
Summary: This study investigated the culture and characteristics of quinoline-degraded aerobic granular sludge (AGS) under different cycle durations. The results showed that an 8-hour cycle duration enhanced the growth of quinoline-degraded AGS and improved settleability and biomass retention. Acidovorax and Paracoccus were dominant bacteria in quinoline degradation in these systems. Additionally, an 8-hour cycle duration resulted in a greater production of extracellular polymeric substances and better total nitrogen removal compared to a 12-hour cycle duration.
ENVIRONMENTAL RESEARCH
(2023)
Article
Environmental Sciences
Zhang-Wei He, Wen-Zong Liu, Cong-Cong Tang, Bin Liang, Ai-Juan Zhou, Fan Chen, Yong-Xiang Ren, Ai-Jie Wang
Summary: The study revealed that BACs accelerated cell lysis and contributed to the accumulation of SCFAs in waste activated sludge. At high concentrations, BACs almost completely inhibited methane production and shifted microbial communities related to SCFAs and methane productions.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Agricultural Engineering
Zhe Liu, Xuhua Zhang, Shumin Zhang, Hao Qi, Yiwen Hou, Min Gao, Jiaxuan Wang, Aining Zhang, Yiping Chen, Yongjun Liu
Summary: Adding polymeric ferric sulfate, aluminum sulfate, and diatomite can enhance aerobic granulation and improve wastewater treatment efficiency under low organic loading conditions. These carriers also increase the production of extracellular polymeric substances and influence the composition of microbial communities.
BIORESOURCE TECHNOLOGY
(2022)
Article
Environmental Sciences
Nathan Pacheco Amin Vieira da Costa, Nelson Libardi, Rejane Helena Ribeiro da Costa
Summary: The ability of extracellular polymeric substances (EPS) recovered from aerobic granular sludge (AGS) to act as bioflocculant was tested in a pilot-scale sequencing batch reactor (SBR), fed with low-strength municipal wastewater. EPS were compared with the addition of Na-alginate as a standardized biopolymer. The addition of both EPS and Na-alginate led to increased adhesion of particles and improved settling biomass in the reactor.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2022)
Article
Engineering, Environmental
Chen Wang, Wei Wei, Yu-Ting Zhang, Xiaohu Dai, Bing-Jie Ni
Summary: The inhibitory effect of different sizes of polystyrene microplastics (PS-MPs) on an anaerobic granular sludge (AGS) wastewater treatment system was investigated. The results showed that larger PS-MPs had a stronger inhibitory effect on methane production and organic carbon degradation by AGS, as well as causing damage to AGS granules and reduction in acidogens and methanogens populations.
Article
Environmental Sciences
Qiulai He, Zhiyi Xie, Zhidong Fu, Hongyu Wang, Li Chen, Shuxian Gao, Wei Zhang, Jianyang Song, Peng Xu, Jian Yu, Jingwei Ma
Summary: The impact of phenol on aerobic granular sludge was investigated in terms of biological phosphorus removal and microbial community structures. Elevated phenol concentrations not only stimulated biological phosphorus removal but also enhanced the production of extracellular polymeric substances, maintaining the structural stability of the granules.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Agricultural Engineering
Zhe Liu, Rushuo Yang, Dan Zhang, Jiaxuan Wang, Min Gao, Aining Zhang, Wenlong Liu, Yongjun Liu
Summary: This study investigated the effect of particulate organic matter (POM) on sludge granulation under low organic load. The results showed that POM promoted the formation of aerobic granular sludge (AGS) and enhanced the sludge settleability and biomass retention. However, when the POM content exceeded a certain level, the sludge performance deteriorated. The analysis revealed that POM suppressed the secretion of extracellular protein and affected the microbial community.
BIORESOURCE TECHNOLOGY
(2023)
Article
Environmental Sciences
Gonglei Wang, Xiaoxiao Huang, Shuai Wang, Fan Yang, Shiting Sun, Peng Yan, Youpeng Chen, Fang Fang, Jinsong Guo
Summary: The food-to-microorganism ratio (F/M) is an important parameter that affects the settleability of aerobic granular sludge (AGS). This study found a relationship between F/M and the sludge volume index (SVI), and identified Thiothrix and Candidatus_Competibacter as dominant bacteria influencing AGS settling performance. The study also showed that an increase in F/M led to a decrease in AGS settling performance, but high levels of gellike polysaccharides effectively retained the granular biomass, resulting in the recovery of AGS settleability.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2023)
Article
Biotechnology & Applied Microbiology
Yiran Qu, Innocent Bekard, Ben Hunt, Jamie Black, Louis Fabri, Sally L. Gras, Sandra. E. Kentish
Summary: This study compares the performance of a nanofiber device and a resin column for antibody capture. The nanofiber device has a larger housing volume and lower binding capacity, but comparable eluate purity to the resin column. It shows high stability, can be used for multiple cycles, and maintains consistent eluate quality when scaled up. The use of a single nanofiber device can significantly reduce costs compared to a resin column, especially when the number of batches is limited.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Brandon Moore, Christos Georgakis, Chris Antoniou, Sarwat Khattak
Summary: Fed-batch cell culture processes are commonly used in biomanufacturing due to their simplicity and applicability in cGMP environments. However, the challenge lies in the changing physiochemical conditions within the bioreactor as the cell density changes. Traditional response surface models (RSMs) are commonly used for optimization but are limited by their use of time-invariant factors. Dynamic RSM (DRSM) models can predict the time-dependent impact of process inputs, allowing for optimization of process operations that change over time.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Lin Li, Yunfan Bai, Chuhua Qi, Yile Du, Xiaoxiao Ma, Yutong Li, Pingping Wu, Shuangli Chen, Sijing Zhang
Summary: A succinic anhydride-modified apple pomace (SAMAP) was synthesized to address environmental issues caused by the accumulation of apple pomace and effectively treat heavy metal ions. SAMAP exhibited high adsorption capacity for Cu(II) and Pb(II), suggesting its potential application in wastewater treatment.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Peter Satzer
Summary: Water for injection (WFI) production in the biopharmaceutical industry consumes excessive amounts of water and energy. Recycling buffers can potentially save up to 90% of resources, but achieving the full theoretical potential is impossible when a risk-aware design is used. Universal risk-based assessment is important for regulatory authorities to consider the implementation of such a strategy.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Gaoya Sun, Lingkai Jin, Guangxue Wang, Xiaoge Wang, Jin Huang
Summary: In this study, heterologous expression and homologous overexpression of ABC transporter proteins AatA and MdlB were found to improve butyric acid production in C. tyrobutyricum. The overexpression of these proteins upregulated the expression levels of key enzymes in the acetate synthesis pathway and promoted the synthesis and secretion of acetic acid. Additionally, the increase in ATPase activity facilitated sugar utilization, induced extracellular secretion of acetate, and shortened fermentation periods.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Taiki Okamura, Rina Aritomi, Takuya Matsumoto, Ryosuke Yamada, Hidehiko Hirakawa, Hiroyasu Ogino
Summary: In this study, proline was introduced to improve the stability of putidaredoxin reductase (PdR) in the Pseudomonas putida cytochrome P450 system. It was found that PdR_T221P had a longer half-life at high temperatures compared to wild-type PdR, but a shorter half-life in the presence of methanol. Molecular dynamics simulations supported these findings.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Yuying Hu, Xiaofan Wang, Shihao Zhang, Zimu Liu, Tengfang Hu, Xin Wang, Xiaoming Peng, Hongling Dai, Jing Wu, Fengping Hu
Summary: This study investigates the effect of iron-carbon micro-electrolysis (ICME) materials on high-solid anaerobic digestion (HSAD). The results show that ICME materials promote methane production in HSAD by increasing the attachment area of microorganisms and facilitating symbiotic metabolism of certain bacterial species. This study provides new insights into microbial mechanisms and enhances our understanding of ICME material enhancement in HSAD.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Jibao Liu, Yufeng Xu, Yuansong Wei
Summary: This study investigated the role of sludge rheology in anaerobic digestion (AD) and found that rheological properties increased with the increase of solid content, resulting in a negative effect on methane production. An extended ADM1 model revealed that enhanced sludge rheological properties increased mass diffusion resistance and reduced uptake rate of acetate.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Xiaoyan Liu, Zongze Chen, Dewen Kong, Xinying Zhang, Chuanhua Wang, Yongqi Wang
Summary: This study explored the role of intracellular and extracellular enzymes of Acinetobacter baumannii and Talaromyces sp. in the degradation of crude oil. The extracellular enzymes of Talaromyces sp. were more effective in degrading n-alkanes, while those of Acinetobacter baumannii had a better effect on aromatic hydrocarbons. The degradation enzyme systems of both bacteria and fungi complemented each other, improving the overall degradation ability.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Jing Dong, Lingli Xu, Yuxiang Liu, Li Ren, Ke Yuan
Summary: The utilization of biochar-immobilized microorganisms is an effective method for eliminating phenol from water. The high susceptibility of bacteria to environmental factors is a challenge for practical implementation. In this study, biochar was used to reduce microbial susceptibility and enhance phenol removal. The addition of biochar altered the dominant species of phenol-degrading bacteria and response surface analysis indicated the significant influence of biochar pyrolysis temperature and experimental temperature on phenol removal rate.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Zibin Pan, Mengying Liu, Zuliang Chen
Summary: This study successfully removed metalloids and heavy metals from acid mine drainage (AMD) using bio-synthesized Fe/Cu nanoparticles (Fe/Cu NPs). The Fe/Cu NPs showed high removal capacities and the presence of organic substances contributed to their stability.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Guangbing Liu, Han Zhang, Jincan Huang, Lu Zhang, Teng Zhang, Xuemin Yu, Weijing Liu, Chunkai Huang
Summary: This study investigated the effect of Fenton pre-treatment on the treatment efficiency of printing and dyeing wastewater (PDW) using two anaerobic/aerobic-membrane bioreactors (A/O-MBRs). The results showed that Fenton pre-treatment significantly improved the removal efficiency of COD and AOX in PDW, and reduced membrane fouling. The Shannon indices and metagenomics analysis indicated that the microbial diversity in anaerobic flocs was higher than that in aerobic flocs, and EC3.1.1.45 and pcaI were identified as key functional genes.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Lidia Borgosz, Duygu Dikicioglu
Summary: The Industrial Internet of Things (IIoT) is a system that connects devices and provides real-time insight into industrial processes. However, the complexity and regulatory requirements of the biomanufacturing sector make it challenging to implement IIoT. There is a need for universal solutions to overcome this challenge and advance the field of biomanufacturing.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Yajie Li, Weikang Kong, Yuyao Zhang, Huarui Zhou, Hongbo Liu, Salma Tabassum
Summary: In this study, the iron-carbon multi-micro electric field coupling anaerobic co-digestion technique was used to treat coal gasification wastewater (CGW). The experimental results showed that under optimal operating conditions, this technique can significantly reduce the toxicity of the wastewater and achieve high removal efficiencies. Additionally, the analysis of microbial communities revealed that the coupling system promotes direct interspecies electron transfer.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Aparecido Nivaldo Modenes, Debora Gozzi Fernandes, Daniela Estelita Goes Trigueros, Matheus Guilherme Amador, Fernando Rodolfo Espinoza-Quinones, Taysa de Souza Braniz, Adilson Ricken Schuelter, Glacy Jaqueline da Silva, Lucimar Pereira Bonett
Summary: This study aimed to systematically remove organic pollutants from raw dairy wastewater with high concentrations of COD, TOC, and TN using Poterioochromonas malhamensis algae strains. The results showed that the biomass yield rate using FP-PBRs was 10% higher than tubular PBRs, and the organic pollution in wastewater was significantly reduced with a decrease of about 98% in COD, 95% in TN, and 92% in TOC.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)