On the difficulties of characterizing weak protein interactions that are critical for neurotransmitter release

Munc13 structural transitions and oligomers that may choreograph successive stages in vesicle priming for neurotransmitter release

(2022) Kirill Grushin et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Molecular Mechanisms Underlying Neurotransmitter Release

(2022) Josep Rizo   Annual Review of Biophysics

Inhibition of calcium-triggered secretion by hydrocarbon-stapled peptides

(2022) Ying Lai et al.   NATURE

All-atom molecular dynamics simulations of Synaptotagmin-SNARE-complexin complexes bridging a vesicle and a flat lipid bilayer

(2022) Josep Rizo et al.   eLife

SNARE assembly enlightened by cryo-EM structures of a synaptobrevin–Munc18-1–syntaxin-1 complex

(2022) Karolina P. Stepien et al.   Science Advances

Synaptotagmin-1–, Munc18-1–, and Munc13-1–dependent liposome fusion with a few neuronal SNAREs

(2021) Karolina P. Stepien et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Chaperoning SNARE Folding and Assembly

(2021) Yongli Zhang et al.   Annual Review of Biochemistry

Evaluation of the tert-butyl group as a probe for NMR studies of macromolecular complexes

(2021) Rashmi Voleti et al.   JOURNAL OF BIOMOLECULAR NMR

Highly accurate protein structure prediction with AlphaFold

(2021) John Jumper et al.   NATURE

Synaptotagmin 1 oligomerization via the juxtamembrane linker regulates spontaneous and evoked neurotransmitter release

(2021) Kevin C. Courtney et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Control of neurotransmitter release by two distinct membrane-binding faces of the Munc13-1 C1C2B region

(2021) Marcial Camacho et al.   eLife

Synaptotagmin 1 oligomers clamp and regulate different modes of neurotransmitter release

(2020) Erica Tagliatti et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Re‐examining how Munc13‐1 facilitates opening of syntaxin‐1

(2020) Magdalena Magdziarek et al.   PROTEIN SCIENCE

Complexin Suppresses Spontaneous Exocytosis by Capturing the Membrane-Proximal Regions of VAMP2 and SNAP25

(2020) Jörg Malsam et al.   Cell Reports

Ca2+-dependent release of synaptotagmin-1 from the SNARE complex on phosphatidylinositol 4,5-bisphosphate-containing membranes

(2020) Rashmi Voleti et al.   eLife

Munc13 binds and recruits SNAP25 to chaperone SNARE complex assembly

(2020) Ramalingam Venkat Kalyana Sundaram et al.   FEBS LETTERS

The control of release probability at nerve terminals

(2019) Jeremy S. Dittman et al.   NATURE REVIEWS NEUROSCIENCE

Munc18 and Munc13 serve as a functional template to orchestrate neuronal SNARE complex assembly

(2019) Shen Wang et al.   Nature Communications

Multiple factors maintain assembled trans-SNARE complexes in the presence of NSF and aSNAP

(2019) Eric A Prinslow et al.   eLife

Membrane bridging by Munc13-1 is crucial for neurotransmitter release

(2019) Bradley Quade et al.   eLife

Munc18-1 is crucial to overcome the inhibition of synaptic vesicle fusion by αSNAP

(2019) Karolina P. Stepien et al.   Nature Communications

Munc13-1 MUN domain and Munc18-1 cooperatively chaperone SNARE assembly through a tetrameric complex

(2019) Tong Shu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Molecular Mechanisms of Fast Neurotransmitter Release

(2018) Axel T. Brunger et al.   Annual Review of Biophysics

Arrest oftrans-SNARE zippering uncovers loosely and tightly docked intermediates in membrane fusion

(2018) Halenur Yavuz et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Synaptotagmin oligomers are necessary and can be sufficient to form a Ca2+ -sensitive fusion clamp

(2018) Sathish Ramakrishnan et al.   FEBS LETTERS

Dynamically Primed Synaptic Vesicle States: Key to Understand Synaptic Short-Term Plasticity

(2018) Erwin Neher et al.   NEURON

Munc18-1 catalyzes neuronal SNARE assembly by templating SNARE association

(2018) Junyi Jiao et al.   eLife

UNC-18 and Tomosyn Antagonistically Control Synaptic Vesicle Priming Downstream of UNC-13 inCaenorhabditis elegans

(2017) Seungmee Park et al.   JOURNAL OF NEUROSCIENCE

A cascade of multiple proteins and lipids catalyzes membrane fusion

(2017) William Wickner et al.   MOLECULAR BIOLOGY OF THE CELL

The primed SNARE–complexin–synaptotagmin complex for neuronal exocytosis

(2017) Qiangjun Zhou et al.   NATURE

Synaptic weight set by Munc13-1 supramolecular assemblies

(2017) Hirokazu Sakamoto et al.   NATURE NEUROSCIENCE

Stable Positioning of Unc13 Restricts Synaptic Vesicle Fusion to Defined Release Sites to Promote Synchronous Neurotransmission

(2017) Suneel Reddy-Alla et al.   NEURON

Molecular Mechanisms of Synaptic Vesicle Priming by Munc13 and Munc18

(2017) Ying Lai et al.   NEURON

Exceptionally tight membrane-binding may explain the key role of the synaptotagmin-7 C2A domain in asynchronous neurotransmitter release

(2017) Rashmi Voleti et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Morphologies of synaptic protein membrane fusion interfaces

(2017) Preeti Gipson et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Heterodimerization of Munc13 C2A domain with RIM regulates synaptic vesicle docking and priming

(2017) Marcial Camacho et al.   Nature Communications

Munc13-1 and Munc18-1 together prevent NSF-dependent de-priming of synaptic vesicles

(2017) Enqi He et al.   Nature Communications

A synaptotagmin suppressor screen indicates SNARE binding controls the timing and Ca2+ cooperativity of vesicle fusion

(2017) Zhuo Guan et al.   eLife

Reconciling isothermal titration calorimetry analyses of interactions between complexin and truncated SNARE complexes

(2017) Eric A Prinslow et al.   eLife

Autoinhibition of Munc18-1 modulates synaptobrevin binding and helps to enable Munc13-dependent regulation of membrane fusion

(2017) Ewa Sitarska et al.   eLife

Mechanistic insights into neurotransmitter release and presynaptic plasticity from the crystal structure of Munc13-1 C1C2BMUN

(2017) Junjie Xu et al.   eLife

Unique Structural Features of Membrane-Bound C-Terminal Domain Motifs Modulate Complexin Inhibitory Function

(2017) David Snead et al.   Frontiers in Molecular Neuroscience

Complexin splits the membrane-proximal region of a single SNAREpin

(2016) L. Yin et al.   BIOCHEMICAL JOURNAL

C-terminal domain of mammalian complexin-1 localizes to highly curved membranes

(2016) Jihong Gong et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

N-terminal domain of complexin independently activates calcium-triggered fusion

(2016) Ying Lai et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Synaptotagmin-1 C2B domain interacts simultaneously with SNAREs and membranes to promote membrane fusion

(2016) Shen Wang et al.   eLife

Functional synergy between the Munc13 C-terminal C1 and C2 domains

(2016) Xiaoxia Liu et al.   eLife

PtdInsP2 and PtdSer cooperate to trap synaptotagmin-1 to the plasma membrane in the presence of calcium

(2016) Ángel Pérez-Lara et al.   eLife

Architecture of the synaptotagmin–SNARE machinery for neuronal exocytosis

(2015) Qiangjun Zhou et al.   NATURE

Syntaxin opening by the MUN domain underlies the function of Munc13 in synaptic-vesicle priming

(2015) Xiaoyu Yang et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Synaptotagmin-1 binds to PIP2-containing membrane but not to SNAREs at physiological ionic strength

(2015) Yongsoo Park et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Dynamic binding mode of a Synaptotagmin-1–SNARE complex in solution

(2015) Kyle D Brewer et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

A direct role for the Sec1/Munc18-family protein Vps33 as a template for SNARE assembly

(2015) R. W. Baker et al.   SCIENCE

Syntaxin opening by the MUN domain underlies the function of Munc13 in synaptic-vesicle priming

(2015) Xiaoyu Yang et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Synaptotagmin-1 binds to PIP2-containing membrane but not to SNAREs at physiological ionic strength

(2015) Yongsoo Park et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Dynamic binding mode of a Synaptotagmin-1–SNARE complex in solution

(2015) Kyle D Brewer et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

An Extended Helical Conformation in Domain 3a of Munc18-1 Provides a Template for SNARE (SolubleN-Ethylmaleimide-sensitive Factor Attachment Protein Receptor) Complex Assembly

(2014) Daniel Parisotto et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Calcium sensitive ring-like oligomers formed by synaptotagmin

(2014) J. Wang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Membrane curvature sensing by the C-terminal domain of complexin

(2014) David Snead et al.   Nature Communications

The accessory helix of complexin functions by stabilizing central helix secondary structure

(2014) Daniel T Radoff et al.   eLife

Re-examining how complexin inhibits neurotransmitter release

(2014) Thorsten Trimbuch et al.   eLife

Analysis of SNARE Complex/Synaptotagmin-1 Interactions by One-Dimensional NMR Spectroscopy

(2013) Amy Zhou et al.   BIOCHEMISTRY

Subtle Interplay between Synaptotagmin and Complexin Binding to the SNARE Complex

(2013) Junjie Xu et al.   JOURNAL OF MOLECULAR BIOLOGY

Synaptotagmin-1 and Synaptotagmin-7 Trigger Synchronous and Asynchronous Phases of Neurotransmitter Release

(2013) Taulant Bacaj et al.   NEURON

Neurotransmitter Release: The Last Millisecond in the Life of a Synaptic Vesicle

(2013) Thomas C. Südhof   NEURON

Prevalent mechanism of membrane bridging by synaptotagmin-1

(2013) Alpay B. Seven et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The Membrane Fusion Enigma: SNAREs, Sec1/Munc18 Proteins, and Their Accomplices—Guilty as Charged?

(2012) Josep Rizo et al.   Annual Review of Cell and Developmental Biology

Solution single-vesicle assay reveals PIP2-mediated sequential actions of synaptotagmin-1 on SNAREs

(2012) Jae-Yeol Kim et al.   EMBO JOURNAL

The Presynaptic Active Zone

(2012) Thomas C. Südhof   NEURON

Reconstitution of the Vital Functions of Munc18 and Munc13 in Neurotransmitter Release

(2012) C. Ma et al.   SCIENCE

Short-Term Presynaptic Plasticity

(2012) W. G. Regehr   Cold Spring Harbor Perspectives in Biology

Phosphatidylinositol 4,5-Bisphosphate Alters Synaptotagmin 1 Membrane Docking and Drives Opposing Bilayers Closer Together

(2011) Weiwei Kuo et al.   BIOCHEMISTRY

Complexin Maintains Vesicles in the Primed State in C. elegans

(2011) Robert J. Hobson et al.   CURRENT BIOLOGY

Complexin Has Opposite Effects on Two Modes of Synaptic Vesicle Fusion

(2011) Jesse A. Martin et al.   CURRENT BIOLOGY

Comparative Lipidomic Analysis of Mouse and Human Brain with Alzheimer Disease

(2011) Robin B. Chan et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Synaptotagmin-1 may be a distance regulator acting upstream of SNARE nucleation

(2011) Geert van den Bogaart et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Munc13 mediates the transition from the closed syntaxin–Munc18 complex to the SNARE complex

(2011) Cong Ma et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Complexin cross-links prefusion SNAREs into a zigzag array

(2011) Daniel Kümmel et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Complexin activates and clamps SNAREpins by a common mechanism involving an intermediate energetic state

(2011) Feng Li et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

RIM Proteins Activate Vesicle Priming by Reversing Autoinhibitory Homodimerization of Munc13

(2011) Lunbin Deng et al.   NEURON

In vitro system capable of differentiating fast Ca2+-triggered content mixing from lipid exchange for mechanistic studies of neurotransmitter release

(2011) M. Kyoung et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Binding of Munc18-1 to Synaptobrevin and to the SNARE Four-Helix Bundle

(2010) Yi Xu et al.   BIOCHEMISTRY

At the junction of SNARE and SM protein function

(2010) Chavela M Carr et al.   CURRENT OPINION IN CELL BIOLOGY

HOPS prevents the disassembly of trans-SNARE complexes by Sec17p/Sec18p during membrane fusion

(2010) Hao Xu et al.   EMBO JOURNAL

Synaptotagmin 1 and SNAREs Form a Complex That Is Structurally Heterogeneous

(2010) Alex L. Lai et al.   JOURNAL OF MOLECULAR BIOLOGY

Binding of the complexin N terminus to the SNARE complex potentiates synaptic-vesicle fusogenicity

(2010) Mingshan Xue et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Munc13 C2B domain is an activity-dependent Ca2+ regulator of synaptic exocytosis

(2010) Ok-Ho Shin et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Doc2b Is a High-Affinity Ca2+ Sensor for Spontaneous Neurotransmitter Release

(2010) A. J. Groffen et al.   SCIENCE

Modular architecture of Munc13/calmodulin complexes: dual regulation by Ca2+ and possible function in short-term synaptic plasticity

(2009) Fernando Rodríguez-Castañeda et al.   EMBO JOURNAL

A role of complexin-lipid interactions in membrane fusion

(2009) Florian Seiler et al.   FEBS LETTERS

The Ca2+Affinity of Synaptotagmin 1 Is Markedly Increased by a Specific Interaction of Its C2B Domain with Phosphatidylinositol 4,5-Bisphosphate

(2009) Anand Radhakrishnan et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Tilting the Balance between Facilitatory and Inhibitory Functions of Mammalian and Drosophila Complexins Orchestrates Synaptic Vesicle Exocytosis

(2009) Mingshan Xue et al.   NEURON

Complexin Controls the Force Transfer from SNARE Complexes to Membranes in Fusion

(2009) A. Maximov et al.   SCIENCE

Munc18a controls SNARE assembly through its interaction with the syntaxin N-peptide

(2008) Pawel Burkhardt et al.   EMBO JOURNAL

NMR analysis of the closed conformation of syntaxin-1

(2008) Xiaocheng Chen et al.   JOURNAL OF BIOMOLECULAR NMR

The Janus-faced nature of the C2B domain is fundamental for synaptotagmin-1 function

(2008) Mingshan Xue et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Complexins facilitate neurotransmitter release at excitatory and inhibitory synapses in mammalian central nervous system

(2008) M. Xue et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Conformational Switch of Syntaxin-1 Controls Synaptic Vesicle Fusion

(2008) Stefan H. Gerber et al.   SCIENCE