Article
Biochemistry & Molecular Biology
Siting Wu, Mengshi Sun, Xin Zhang, Jiaming Liao, Mengke Liu, Qiwei Qin, Jingguang Wei
Summary: The study characterized the EcTRAF4 homologue in orange-spotted grouper and found that it plays crucial roles in RGNNV infection process, promoting viral replication and interacting with RGNNV coat protein to inhibit IFN signaling and reduce inflammatory responses.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Fisheries
Xuzhuo Duan, Kaishan Liang, Min Yang, Minlin Zhang, Xiaoling Zuo, Xianze Jia, Zongyang Li, Jie Yu, Liyuan Luo, Jinhong Shan, Huihong Zhao, Yong Zhang, Qiwei Qin, Qing Wang
Summary: The study conducted a genome-wide association study on the resistance of giant groupers to red-spotted grouper nervous necrosis virus (RGNNV), identifying SNP loci and candidate genes that are significantly associated with RGNNV resistance. G protein-coupled receptor 143 (gpr143) was identified as a candidate gene for disease resistance in giant groupers.
Article
Immunology
Mengshi Sun, Siting Wu, Shaozhu Kang, Jiaming Liao, Luhao Zhang, Zhuqing Xu, Hong Chen, Linting Xu, Xin Zhang, Qiwei Qin, Jingguang Wei
Summary: This study found that the infection of red-spotted grouper nervous necrosis virus (RGNNV) induced the formation of stress granules (SGs) in grouper spleen cells. The SGs particles co-located with the classic SG marker protein eIF3 eta, and some SGs depolymerized under treatment with a translation inhibitor. Inhibition of the PERK kinase significantly inhibited the formation of SGs and decreased the phosphorylation of eIF2 alpha. Overexpression of G3BP1 in Epinephelus coioides inhibited the replication of RGNNV by up-regulating the interferon and inflammatory response.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Fisheries
Min Yang, Qing Wang, Jinpeng Chen, Yuxin Wang, Yong Zhang, Qiwei Qin
Summary: The red-spotted grouper, a commercially important species in China and Southeast Asia, faces a serious threat from RGNNV disease outbreaks. By conducting a GWAS, researchers identified candidate genes involved in nervous system development, retinal formation, and lipid metabolism regulation, suggesting potential pathways to enhance disease resistance. This study provides insights for marker-assisted selection in breeding programs aiming to improve red-spotted grouper's resilience to RGNNV.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Fisheries
Xixi Guo, Wenji Wang, Qi Zheng, Qiwei Qin, Youhua Huang, Xiaohong Huang
Summary: This study compared the transcriptomic analysis of SGIV and RGNNV infected grouper cells, revealing the different cell death mechanisms and host immune responses caused by these two viruses. The results also showed the important roles of cytoskeleton and MAPK signaling pathways in SGIV infection, as well as the relationship between post-translational modifications of MAPK signaling-related proteins and viral replication and vacuole formation during RGNNV infection.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Xuzhuo Duan, Xianze Jia, Kaishan Liang, Fengqi Huang, Jinhong Shan, Huitao Chen, Xinhe Ruan, Lihua Li, Huihong Zhao, Qing Wang
Summary: In this study, the researchers successfully induced testis maturation in red-spotted grouper by injecting recombinant plasmids into the immature testis. The results showed that the testis developed from the immature to the mature state within 7 days after injection, and the spermatozoa concentration and motility in injected fish were comparable to naturally mature fish.
MARINE BIOTECHNOLOGY
(2022)
Article
Fisheries
Yoichiro Kitani, Yuto Osaka, Shoichiro Ishizaki
Summary: LAOs in the serum of red-spotted grouper can kill bacterial pathogens through generated hydrogen peroxide. The activation of LAO can be triggered by seawater, altering its secondary structure. This suggests that LAOs in the grouper's serum serve as a defense mechanism against pathogen invasion when mixed with seawater.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Jinpeng Chen, Xinshuai Li, Jianling Huang, Qing Wang, Shaowen Wang, Shina Wei, Qiwei Qin, Min Yang
Summary: In this study, the transcriptional regulation of Epinephelus akaara RFX5 (EaRFX5) on EaMHCI and its effect on immune pathways were investigated. The results showed that EaRFX5 plays a differentiated immunomodulatory role in response to various stimuli in different tissues, with the most significant upregulation in the kidney after SGIV challenge. In addition, EaRFX5 appeared to promote the expression of EaMHCIa gene, interferon signaling pathway and inflammatory cytokine.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Fengqi Huang, Jinhong Shan, Kaishan Liang, Min Yang, Xiaoming Zhou, Xuzhuo Duan, Xianze Jia, Huihong Zhao, Qiwei Qin, Qing Wang
Summary: A rapid detection method for Red-spotted grouper nervous necrosis virus (RGNNV) based on the CRISPR-Cas13a system was developed in this study. The method showed high sensitivity and specificity, providing a theoretical basis for field detection of RGNNV.
Article
Microbiology
Qing Wang, Cheng Peng, Min Yang, Fengqi Huang, Xuzhuo Duan, Shaowen Wang, Huitao Cheng, Huirong Yang, Huihong Zhao, Qiwei Qin
Summary: The study uncovered that RGNNV primarily targeted the midbrain of orange-spotted grouper, leading to neuronal damage. Single-cell RNA-seq analysis identified 35 cell subtypes, with enriched macrophages in infected fish showing acute inflammatory responses. Pseudotime analysis revealed the transformation of microglia into activated macrophages to reduce nerve tissue damage caused by the virus.
Article
Fisheries
Min Yang, Jinpeng Chen, Xinshuai Li, Jianling Huang, Qing Wang, Shaowen Wang, Shina Wei, Qiwei Qin
Summary: This study characterized NFYC and MHCIa in red-spotted grouper, revealing that EaNFYC can regulate the MHC pathway by activating EaMHCIa as well as interferon signalling molecules and inflammatory cytokines. Additionally, EaNFYC exerts its effect on a specific region of the EaMHCIa promoter and activates MHCIa expression through binding at specific binding sites. These findings contribute to a better understanding of the regulatory mechanisms of NFYC in fish MHC transcription.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Wengang Xu, Huafeng Zou, Jun Zeng, Weiping Mei, SongHee Choi
Summary: This study investigated the effects of light spectra exposure on the growth and reproduction of juvenile red spotted grouper. The fish exposed to white LEDs exhibited the best growth, while the expression levels of growth hormone (GH) showed no significant differences and the pre-pro-somatostatin-I (PSS-I) expression levels significantly increased. All four LED spectra were effective in stimulating food intake. The gonadal development advanced in all four LED groups, while there were no significant differences in FSHβ and LHβ expression.
Article
Biochemistry & Molecular Biology
Yue-Xuan Wang, Sui-Feng Xu, Ye-Wen Wang, Yun-Xiang Jiang, Qi-Wei Qin, Shi-Na Wei
Summary: This study aimed to investigate the protective effect of curcumin against Singapore grouper iridovirus-induced intestinal damage in orange-spotted groupers. The results showed that curcumin significantly alleviated intestine damage, repaired intestinal barrier dysfunction, suppressed intestinal cells apoptosis and inflammatory response, protected intestinal cells from oxidative injury, and restored the disruption of the intestinal microbiota caused by the virus. These findings provide a theoretical basis for the use of curcumin in aquaculture to prevent SGIV-induced intestinal injury.
Article
Marine & Freshwater Biology
Byeong-Hoon Kim, Sung-Pyo Hur, Song-Hee Choi, Young-Don Lee, Chi-Hoon Lee
Summary: This study aimed to explore suitable rearing water temperatures for minimizing water temperature stress and promoting growth in farmed red-spotted groupers. The results showed that July to September 2020 was the period with the fastest body growth (body weight and total length). In August 2020, weight gain, specific growth rate, and feed efficiency were the highest. The findings suggest that a water temperature of 26 degrees Celsius is suitable for farmed red-spotted groupers.
OCEAN SCIENCE JOURNAL
(2023)
Article
Fisheries
Weijun Mai, Bingjun Yan, Jian Xin
Summary: In this study, a vaccine utilizing Bacillus subtilis as a vehicle was developed to protect grouper against Nervous Necrosis Virus (NNV) infection. The vaccine induced enhanced non-specific immune responses, specific antibody production, and up-regulation of immune-related genes in the grouper. The highest dose of bath vaccination showed the most promising results, with significantly reduced mortality after NNV challenge. The vaccine demonstrated high levels of mucosal and humoral immunity and is a potential candidate for protecting against NNV infection.
