Article
Fisheries
Venkata Satyanarayana Nallala, M. Makesh, K. Radhika, T. Sathish Kumar, P. Raja, R. Subburaj, M. Kailasam, K. K. Vijayan
Summary: In this study, nervous necrosis virus was successfully isolated from asymptomatic wild Asian seabass, and its genome was analyzed. The virus showed infectivity to Asian seabass larvae, causing 100% mortality, making it a potential candidate for developing a vaccine against viral nervous necrosis.
Article
Virology
Zhiqi Zhang, Jing Xing, Xiaoqian Tang, Xiuzhen Sheng, Heng Chi, Wenbin Zhan
Summary: Nervous necrosis virus (NNV) is a significant pathogen in the aquaculture industry, causing substantial financial losses. The capsid protein (CP) is the only structural protein with pathogenicity and antigenicity, but information on immunodominant regions is scarce.
JOURNAL OF VIROLOGY
(2023)
Article
Fisheries
Zhiqi Zhang, Jing Xing, Xiaoqian Tang, Xiuzhen Sheng, Heng Chi, Wenbin Zhan
Summary: This study manufactured abundant RGNNV VLPs and developed three monoclonal antibodies that can accurately detect the capsid protein of RGNNV and show potential in detection and tracking of the virus. Two of the antibodies also possess some neutralizing capacity against RGNNV infection.
Article
Fisheries
Xixi Guo, Wenji Wang, Qi Zheng, Qiwei Qin, Youhua Huang, Xiaohong Huang
Summary: This study compared the transcriptomic analysis of SGIV and RGNNV infected grouper cells, revealing the different cell death mechanisms and host immune responses caused by these two viruses. The results also showed the important roles of cytoskeleton and MAPK signaling pathways in SGIV infection, as well as the relationship between post-translational modifications of MAPK signaling-related proteins and viral replication and vacuole formation during RGNNV infection.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Weijun Mai, Bingjun Yan, Jian Xin
Summary: In this study, a vaccine utilizing Bacillus subtilis as a vehicle was developed to protect grouper against Nervous Necrosis Virus (NNV) infection. The vaccine induced enhanced non-specific immune responses, specific antibody production, and up-regulation of immune-related genes in the grouper. The highest dose of bath vaccination showed the most promising results, with significantly reduced mortality after NNV challenge. The vaccine demonstrated high levels of mucosal and humoral immunity and is a potential candidate for protecting against NNV infection.
Article
Fisheries
Menglan Zhang, Zhijie Lu, MeiZhen Tang, Gan Pan, Lijuan Zhao, Zhendong Qin, Li Lin
Summary: The study found that Epinephelus coioides mannose receptor (EcMR) responds to virus infection by modulating virus-induced apoptosis and inflammation.
Article
Fisheries
Min Yang, Qing Wang, Jinpeng Chen, Yuxin Wang, Yong Zhang, Qiwei Qin
Summary: The red-spotted grouper, a commercially important species in China and Southeast Asia, faces a serious threat from RGNNV disease outbreaks. By conducting a GWAS, researchers identified candidate genes involved in nervous system development, retinal formation, and lipid metabolism regulation, suggesting potential pathways to enhance disease resistance. This study provides insights for marker-assisted selection in breeding programs aiming to improve red-spotted grouper's resilience to RGNNV.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Siting Wu, Mengshi Sun, Xin Zhang, Jiaming Liao, Mengke Liu, Qiwei Qin, Jingguang Wei
Summary: The study characterized the EcTRAF4 homologue in orange-spotted grouper and found that it plays crucial roles in RGNNV infection process, promoting viral replication and interacting with RGNNV coat protein to inhibit IFN signaling and reduce inflammatory responses.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Fisheries
Rahul Krishnan, Rahul Rajendran, Yo-Seb Jang, Jong-Oh Kim, Su-Young Yoon, Myung-Joo Oh
Summary: NLRC3, a unique regulatory NLR, is involved in the modulation of cellular processes and inflammatory responses. In this study, the function of NLRC3 in the context of nervous necrosis virus (NNV) infection in seven band grouper was characterized. NLRC3 was found to enhance NNV replication and attenuate interferon responses, while positively regulating inflammasome response and pro-inflammatory gene expression. NLRC3 also negatively regulates the PI3K-mTOR axis and activates the cellular autophagic response. These findings provide important insights into the regulatory mechanism of NLRC3 in the immune response following NNV infection.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Immunology
Mengshi Sun, Siting Wu, Shaozhu Kang, Jiaming Liao, Luhao Zhang, Zhuqing Xu, Hong Chen, Linting Xu, Xin Zhang, Qiwei Qin, Jingguang Wei
Summary: This study found that the infection of red-spotted grouper nervous necrosis virus (RGNNV) induced the formation of stress granules (SGs) in grouper spleen cells. The SGs particles co-located with the classic SG marker protein eIF3 eta, and some SGs depolymerized under treatment with a translation inhibitor. Inhibition of the PERK kinase significantly inhibited the formation of SGs and decreased the phosphorylation of eIF2 alpha. Overexpression of G3BP1 in Epinephelus coioides inhibited the replication of RGNNV by up-regulating the interferon and inflammatory response.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Fisheries
W. M. Gayashani Sandamalika, D. S. Liyanage, Chaehyeon Lim, Hyerim Yang, Sukkyoung Lee, Taehyug Jeong, Qiang Wan, Jehee Lee
Summary: This study investigates the immune responses of red-spotted groupers upon pathogenic invasion. Transcriptomic profiles were analyzed to identify differentially expressed genes (DEGs) in red-spotted grouper head kidney stimulated with lipopolysaccharide (LPS), polyinosinic:polycytidylic acid (poly I:C), and nervous necrosis virus (NNV). Cluster analysis of the DEGs revealed their distribution in nine separate clusters based on expression patterns. Most DEGs showed significant upregulation 6 hours after poly I:C stimulation. Functional annotation using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis identified several immune-related signaling pathways with significant expression. Quantitative real-time PCR validation confirmed the reliability of selected DEGs. Overall, this study provides valuable insights into the immune responses of red-spotted groupers, enhancing our understanding of fish immunity.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
H. A. C. R. Hanchapola, D. S. Liyanage, W. K. M. Omeka, Chaehyeon Lim, Gaeun Kim, Taehyug Jeong, Jehee Lee
Summary: This study identified and characterized the EaTXNDC12 gene in red spotted grouper, evaluated its transcriptional responses, and investigated the activity of the recombinant protein. The results showed that EaTXNDC12 plays an important role in cellular redox homeostasis and immune regulation.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Rahul Krishnan, Jong-Oh Kim, Yo-Seb Jang, Myung-Joo Oh
Summary: PSMB8 serves as an important regulator of NF-xB signaling during nervous necrosis virus (NNV) infection in sevenband grouper, showing the ability to reduce virus-induced activation of NF-xB promoters and downregulate the expression of proinflammatory cytokines and interferons.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Xuzhuo Duan, Xianze Jia, Kaishan Liang, Fengqi Huang, Jinhong Shan, Huitao Chen, Xinhe Ruan, Lihua Li, Huihong Zhao, Qing Wang
Summary: In this study, the researchers successfully induced testis maturation in red-spotted grouper by injecting recombinant plasmids into the immature testis. The results showed that the testis developed from the immature to the mature state within 7 days after injection, and the spermatozoa concentration and motility in injected fish were comparable to naturally mature fish.
MARINE BIOTECHNOLOGY
(2022)
Article
Fisheries
Sumit Vinod Jungi, Pakkakul Sangsuriya, Suwimon Taengphu, Kornsunee Phiwsaiya, Molruedee Sonthi, Bunlung Nuangsaeng, Krishna R. Salin, Saengchan Senapin, Ha Thanh Dong
Summary: This study identified Nervous Necrosis Virus (NNV) of the RGNNV genotype as a virus associated with massive die-offs in imported pearl grouper fry in Thailand. The virus was found to localize in the vacuolation lesions of the central nervous system and retina of infected fish. Appropriate biosecurity measures are needed to prevent any loss to the marine aquaculture industry considering the infectious nature and broad host range of the virus.