Conformational transitions of the Spindly adaptor underlie its interaction with Dynein and Dynactin

Structure of the RZZ complex and molecular basis of Spindly‐driven corona assembly at human kinetochores

(2022) Tobias Raisch et al.   EMBO JOURNAL

JIP3 interacts with dynein and kinesin-1 to regulate bidirectional organelle transport

(2022) Ricardo Celestino et al.   JOURNAL OF CELL BIOLOGY

The Disordered Spindly C-terminus Interacts with RZZ Subunits ROD-1 and ZWL-1 in the Kinetochore through the Same Sites in C. Elegans

(2021) Morkos A. Henen et al.   JOURNAL OF MOLECULAR BIOLOGY

Structure and Mechanics of Dynein Motors

(2021) John T. Canty et al.   Annual Review of Biophysics

Cryo‐EM reveals the complex architecture of dynactin's shoulder region and pointed end

(2021) Clinton K Lau et al.   EMBO JOURNAL

Expasy, the Swiss Bioinformatics Resource Portal, as designed by its users

(2021) Séverine Duvaud et al.   NUCLEIC ACIDS RESEARCH

Highly accurate protein structure prediction with AlphaFold

(2021) John Jumper et al.   NATURE

Reconstitution and use of highly active human CDK1 : Cyclin‐B : CKS1 complexes

(2021) Pim J. Huis in 't Veld et al.   PROTEIN SCIENCE

CENP-F stabilizes kinetochore-microtubule attachments and limits dynein stripping of corona cargoes

(2020) Philip Auckland et al.   JOURNAL OF CELL BIOLOGY

Kinetochore protein Spindly controls microtubule polarity in Drosophila axons

(2020) Urko del Castillo et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Organizational Principles of the NuMA-Dynein Interaction Interface and Implications for Mitotic Spindle Functions

(2020) Cristina Renna et al.   STRUCTURE

Crowning the Kinetochore: The Fibrous Corona in Chromosome Segregation

(2020) Geert J.P.L. Kops et al.   TRENDS IN CELL BIOLOGY

Quantifying the heterogeneity of macromolecular machines by mass photometry

(2020) Adar Sonn-Segev et al.   Nature Communications

Mechanobiology of the Mitotic Spindle

(2020) Nenad Pavin et al.   DEVELOPMENTAL CELL

A transient helix in the disordered region of dynein light intermediate chain links the motor to structurally diverse adaptors for cargo transport

(2019) Ricardo Celestino et al.   PLOS BIOLOGY

Dynein activators and adaptors at a glance

(2019) Mara A. Olenick et al.   JOURNAL OF CELL SCIENCE

Electroporated recombinant proteins as tools for in vivo functional complementation, imaging and chemical biology

(2019) Amal Alex et al.   eLife

Polo regulates Spindly to prevent premature stabilization of kinetochore–microtubule attachments

(2019) João Barbosa et al.   EMBO JOURNAL

Dynamic kinetochore size regulation promotes microtubule capture and chromosome biorientation in mitosis

(2018) Carlos Sacristan et al.   NATURE CELL BIOLOGY

The cytoplasmic dynein transport machinery and its many cargoes

(2018) Samara L. Reck-Peterson et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Cryo-electron tomography reveals that dynactin recruits a team of dyneins for processive motility

(2018) Danielle A. Grotjahn et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

A conserved interaction of the dynein light intermediate chain with dynein-dynactin effectors necessary for processivity

(2018) In-Gyun Lee et al.   Nature Communications

Cryo-electron tomography reveals that dynactin recruits a team of dyneins for processive motility

(2018) Danielle A. Grotjahn et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Spindly is required for rapid migration of human cells

(2018) Claudia Conte et al.   Biology Open

Simplified Protocol for Cross-linking Mass Spectrometry Using the MS-Cleavable Cross-linker DSBU with Efficient Cross-link Identification

(2018) Dongqing Pan et al.   ANALYTICAL CHEMISTRY

Cryo-EM shows how dynactin recruits two dyneins for faster movement

(2018) Linas Urnavicius et al.   NATURE

Walking Forward with Kinesin

(2018) Eva Klinman et al.   TRENDS IN NEUROSCIENCES

Distinct Roles of RZZ and Bub1-KNL1 in Mitotic Checkpoint Signaling and Kinetochore Expansion

(2018) Jose-Antonio Rodriguez-Rodriguez et al.   CURRENT BIOLOGY

Self-Assembly of the RZZ Complex into Filaments Drives Kinetochore Expansion in the Absence of Microtubule Attachment

(2018) Cláudia Pereira et al.   CURRENT BIOLOGY

Molecular mechanism of dynein recruitment to kinetochores by the Rod–Zw10–Zwilch complex and Spindly

(2017) José B. Gama et al.   JOURNAL OF CELL BIOLOGY

Structure of the RZZ complex and molecular basis of its interaction with Spindly

(2017) Shyamal Mosalaganti et al.   JOURNAL OF CELL BIOLOGY

MolProbity: More and better reference data for improved all-atom structure validation

(2017) Christopher J. Williams et al.   PROTEIN SCIENCE

NuMA recruits dynein activity to microtubule minus-ends at mitosis

(2017) Christina L Hueschen et al.   eLife

The human cytoplasmic dynein interactome reveals novel activators of motility

(2017) William B Redwine et al.   eLife

How dynein and dynactin transport cargos: a structural perspective

(2016) Andrew P Carter et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Hook Adaptors Induce Unidirectional Processive Motility by Enhancing the Dynein-Dynactin Interaction

(2016) Mara A. Olenick et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Assembly and activation of dynein–dynactin by the cargo adaptor protein Hook3

(2016) Courtney M. Schroeder et al.   JOURNAL OF CELL BIOLOGY

biGBac enables rapid gene assembly for the expression of large multisubunit protein complexes

(2016) Florian Weissmann et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Bicaudal D Family of Motor Adaptors: Linking Dynein Motility to Cargo Binding

(2016) Casper C. Hoogenraad et al.   TRENDS IN CELL BIOLOGY

Structural basis for cargo binding and autoinhibition of Bicaudal-D1 by a parallel coiled-coil with homotypic registry

(2015) Shin-ichi Terawaki et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Microtubule Minus-End-Targeting Proteins

(2015) Anna Akhmanova et al.   CURRENT BIOLOGY

The Molecular Biology of Spindle Assembly Checkpoint Signaling Dynamics

(2015) Andrea Musacchio   CURRENT BIOLOGY

A novel role of farnesylation in targeting a mitotic checkpoint protein, human Spindly, to kinetochores

(2015) Devinderjit K. Moudgil et al.   JOURNAL OF CELL BIOLOGY

Preventing farnesylation of the dynein adaptor Spindly contributes to the mitotic defects caused by farnesyltransferase inhibitors

(2015) Andrew J. Holland et al.   MOLECULAR BIOLOGY OF THE CELL

Adaptive changes in the kinetochore architecture facilitate proper spindle assembly

(2015) Valentin Magidson et al.   NATURE CELL BIOLOGY

Structural organization of the dynein–dynactin complex bound to microtubules

(2015) Saikat Chowdhury et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

xVis: a web server for the schematic visualization and interpretation of crosslink-derived spatial restraints

(2015) Maximilian Grimm et al.   NUCLEIC ACIDS RESEARCH

The structure of the dynactin complex and its interaction with dynein

(2015) L. Urnavicius et al.   SCIENCE

Structural organization of the dynein–dynactin complex bound to microtubules

(2015) Saikat Chowdhury et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

MASSIF-1: a beamline dedicated to the fully automatic characterization and data collection from crystals of biological macromolecules

(2015) Matthew W. Bowler et al.   JOURNAL OF SYNCHROTRON RADIATION

In vitroreconstitution of a highly processive recombinant human dynein complex

(2014) Max A Schlager et al.   EMBO JOURNAL

Activation of cytoplasmic dynein motility by dynactin-cargo adapter complexes

(2014) R. J. McKenney et al.   SCIENCE

Bicaudal D Family Adaptor Proteins Control the Velocity of Dynein-Based Movements

(2014) Max A. Schlager et al.   Cell Reports

A Ras-like domain in the light intermediate chain bridges the dynein motor to a cargo-binding region

(2014) Courtney M Schroeder et al.   eLife

ThePDB_REDOserver for macromolecular structure model optimization

(2014) Robbie P. Joosten et al.   IUCrJ

Bicaudal-D uses a parallel, homodimeric coiled coil with heterotypic registry to coordinate recruitment of cargos to dynein

(2013) Y. Liu et al.   GENES & DEVELOPMENT

Systematic dissection of dynein regulators in mitosis

(2013) Jonne A. Raaijmakers et al.   JOURNAL OF CELL BIOLOGY

Functions and mechanics of dynein motor proteins

(2013) Anthony J. Roberts et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Crosstalk Between Microtubule Attachment Complexes Ensures Accurate Chromosome Segregation

(2013) D. K. Cheerambathur et al.   SCIENCE

RELION: Implementation of a Bayesian approach to cryo-EM structure determination

(2012) Sjors H.W. Scheres   JOURNAL OF STRUCTURAL BIOLOGY

Dynactin's pointed-end complex is a cargo-targeting module

(2012) Ting-Yu Yeh et al.   MOLECULAR BIOLOGY OF THE CELL

BICD2, dynactin, and LIS1 cooperate in regulating dynein recruitment to cellular structures

(2012) Daniël Splinter et al.   MOLECULAR BIOLOGY OF THE CELL

Fiji: an open-source platform for biological-image analysis

(2012) Johannes Schindelin et al.   NATURE METHODS

Designer proteins: applications of genetic code expansion in cell biology

(2012) Lloyd Davis et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Recent advances in theCRANKsoftware suite for experimental phasing

(2011) Navraj S. Pannu et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

REFMAC5 for the refinement of macromolecular crystal structures

(2011) Garib N. Murshudov et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Overview of theCCP4 suite and current developments

(2011) Martyn D. Winn et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Probing Protein-Protein Interactions with a Genetically Encoded Photo-crosslinking Amino Acid

(2011) Hui-wang Ai et al.   CHEMBIOCHEM

Enabling high-throughput ligation-independent cloning and protein expression for the family of ubiquitin specific proteases

(2011) Mark P.A. Luna-Vargas et al.   JOURNAL OF STRUCTURAL BIOLOGY

XDS

(2010) Wolfgang Kabsch   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Features and development ofCoot

(2010) P. Emsley et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Removal of Spindly from microtubule-attached kinetochores controls spindle checkpoint silencing in human cells

(2010) R. Gassmann et al.   GENES & DEVELOPMENT

Spindly/CCDC99 Is Required for Efficient Chromosome Congression and Mitotic Checkpoint Regulation

(2010) Marin Barisic et al.   MOLECULAR BIOLOGY OF THE CELL

Structural Analysis of the RZZ Complex Reveals Common Ancestry with Multisubunit Vesicle Tethering Machinery

(2010) Filiz Çivril et al.   STRUCTURE

The structural basis of Arf effector specificity: the crystal structure of ARF6 in a complex with JIP4

(2009) Tatiana Isabet et al.   EMBO JOURNAL

Dynein light intermediate chain 1 is required for progress through the spindle assembly checkpoint

(2009) Mylavarapu V S Sivaram et al.   EMBO JOURNAL

Mitotic control of kinetochore-associated dynein and spindle orientation by human Spindly

(2009) Ying Wai Chan et al.   JOURNAL OF CELL BIOLOGY

Enzymatic assembly of DNA molecules up to several hundred kilobases

(2009) Daniel G Gibson et al.   NATURE METHODS

A new mechanism controlling kinetochore-microtubule interactions revealed by comparison of two dynein-targeting components: SPDL-1 and the Rod/Zwilch/Zw10 complex

(2008) R. Gassmann et al.   GENES & DEVELOPMENT

Direct role of dynein motor in stable kinetochore-microtubule attachment, orientation, and alignment

(2008) Dileep Varma et al.   JOURNAL OF CELL BIOLOGY

SPDL-1 functions as a kinetochore receptor for MDF-1 inCaenorhabditis elegans

(2008) Takaharu G. Yamamoto et al.   JOURNAL OF CELL BIOLOGY

Dynein Light Intermediate Chain: An Essential Subunit That Contributes to Spindle Checkpoint Inactivation

(2008) Sarah Mische et al.   MOLECULAR BIOLOGY OF THE CELL