4.5 Article

Fungal variegatic acid and extracellular polysaccharides promote the site-specific generation of reactive oxygen species

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出版社

SPRINGER HEIDELBERG
DOI: 10.1007/s10295-016-1889-5

关键词

Serpula lacrymans; Variegatic acid; Fe(III) reduction; Reactive oxygen species; beta-Glucan; Radical scavengers; Fungal extracellular matrix; Fungal sheath

资金

  1. Forestry Industry Research Special Funds for Public Welfare Projects [201204702-B2]
  2. USDA-HATCH Project [S-1041 VA-136288]
  3. Research Council of Norway [243663/E50 BioMim]
  4. China Scholarship Council [201406510014]

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This study aims to clarify the role of variegatic acid (VA) in fungal attack by Serpula lacrymans, and also the generation and scavenging of reactive oxygen species (ROS) by the fungus. VA promotes a mediated Fenton reaction to generated ROS after oxalate solubilizes oxidized forms of iron. The fungal extracellular matrix (ECM) beta-glucan scavenged ROS, and we propose this as a mechanism to protect the fungal hyphae while ROS generation is promoted to deconstruct the lignocellulose cell wall. A relatively high pH (4.4) also favored Fe(III) transfer from oxalate to VA as opposed to a lower pH (2.2) conditions, suggesting a pH-dependent Fe(III) transfer to VA employed by S. lacrymans. This permits ROS generation within the higher pH of the cell wall, while limiting ROS production near the fungal hyphae, while beta-glucan from the fungal ECM scavenges ROS in the more acidic environments surrounding the fungal hyphae.

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