Article
Cell Biology
Fei Ge, Qi Pan, Yue Qin, Mengping Jia, Chengchao Ruan, Xiangxiang Wei, Qing Jing, Xiuling Zhi, Xinhong Wang, Lindi Jiang, Elena Osto, Jieyu Guo, Dan Meng
Summary: This study explored the cellular and molecular properties of aged cardiac vasculature in monkeys and mice. It revealed that the transcription factor BACH1 plays a significant role in regulating endothelial cell senescence and its upregulation exacerbates endothelial cell aging. The findings suggest that BACH1 may serve as a master regulator of aging-related genes in vascular aging.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Letter
Cell & Tissue Engineering
Shaoyang Zhang, Jing Yi Wang, Baojie Li, Feng Yin, Huijuan Liu
Summary: UC-MSCs exhibit heterogeneity during in vitro expansion, but single-cell RNA sequencing has identified two distinct populations of freshly isolated UC-MSCs. These populations show different gene expression patterns, suggesting that they may be suitable for treating different diseases.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Oncology
Kai Chen, Yazhou Wang, Yuting Hou, Qi Wang, Di Long, Xinxin Liu, Xiaodong Tian, Yinmo Yang
Summary: This study characterized the cellular landscape and transcriptional dynamics of T cells in PDAC through single-cell RNA sequencing analysis, identifying interactions between malignant ductal cells and tumor cells. The findings demonstrated that CCL5 promoted tumor cell migration in PDAC by interacting with SDC1.
Article
Oncology
YanJie Lu, HanZheng Zhao, Ying Liu, YanZhen Zuo, Qian Xu, Lei Liu, XiaoMin Li, HongBin Zhu, Ying Zhang, Shuling Zhang, XiangYang Zhao, YuHong Li
Summary: Chronic stress can exacerbate tumor angiogenesis, primarily through elevating stress-related hormone levels and causing behavioral changes in individuals. Utilizing isoprenaline as an agonist of beta 2-adrenergic receptor, the study demonstrated the molecular mechanisms of stress in tumor angiogenesis both in vivo and in vitro. Isoprenaline promotes VEGF autocrine in HUVECs, up-regulating plexinA1 and VEGFR2 levels, leading to activation of the JAK2-STAT3 signaling pathway essential for angiogenesis.
FRONTIERS IN ONCOLOGY
(2021)
Review
Pharmacology & Pharmacy
Bingying Dai, Liqing Ding, Lijuan Zhao, Honglin Zhu, Hui Luo
Summary: This review summarizes the current knowledge on immune cells and stromal cells in SSc patients and discusses their potential roles in the pathogenesis of SSc, with a focus on recent advances in new subtypes by scRNA-seq.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Cell Biology
Fengchan Li, Yun Du, Lei Hong, Ziting Liu, Kunmin Yan, Chu Liu, Zhen Zhu, Qiongyu Lu, Chaojun Tang, Li Zhu
Summary: Research has shown that there is cellular heterogeneity between symptomatic and asymptomatic plaques, with cell adhesion-related genes upregulated in asymptomatic plaques. Additionally, cell adhesion is enriched in stable and non-hemorrhaged plaques, which may be related to plaque stability and hemorrhage.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2022)
Article
Medicine, Research & Experimental
Hailang Fan, Lairong Song, Jian Fan, Junpeng Ma, Xiaojie Li, Junting Zhang, Jian Hu, Zhen Wu, Dake Zhang, Liang Wang
Summary: Analyzing meningioma of distinct pathological types at the single-cell level reveals high heterogeneity and diverse biological functions within neoplastic cells. Proliferative neoplastic cells may educate macrophages, promoting tumorigenesis possibly through the MIF-CD74 interaction, offering novel insights for potential therapeutic avenues.
JOURNAL OF TRANSLATIONAL MEDICINE
(2023)
Article
Cell Biology
Qianqian Ge, Jiajia Wang, Jitao Li, Jian Li
Summary: This study used single cell RNA-sequencing to investigate the specific responses of shrimp gill cells to high pH stress. The results showed that pillar cells were the main target cell type in response to high pH stress, exhibiting immune activation and stress defense states. Additionally, the study predicted the regulatory network of the pillar cell population and identified two transcription factors.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Immunology
Katarzyna Sarad, Monika Stefanska, Izabela Kraszewska, Krzysztof Szade, Judith C. Sluimer, Przemyslaw Blyszczuk, Jozef Dulak, Agnieszka Jazwa-Kusior
Summary: This study analyzed the transcriptome of different Nrf2-deficient macrophage subpopulations from murine atherosclerotic aortas. The results showed that Nrf2 deficiency led to subtype-specific transcriptomic changes associated with inflammation, iron homeostasis, and cell injury or death pathways.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Isabela Gobbo Ferreira, Manuela Berto Pucca, Isadora Sousa de Oliveira, Felipe Augusto Cerni, Beatriz de Cassia da Silva Jacob, Eliane Candiani Arantes
Summary: VEGF is a crucial regulator of angiogenesis in both physiological and pathological conditions. It is categorized into seven families, with different types found in mammals, viruses, and snake venoms. This review focuses on the svVEGF family, exploring its distribution, molecular structure, functions, and potential applications within snake venoms.
CYTOKINE & GROWTH FACTOR REVIEWS
(2021)
Article
Pharmacology & Pharmacy
Rei Mishima, Masayuki Taniguchi, Kazutoshi Matsushita, Bowen Tian, Tomoyuki Furuyashiki
Summary: Using single-cell RNA sequencing, distinct microglial subpopulations with different transcriptome signatures were identified in the resting brain. The distribution of these subpopulations varied across brain regions, particularly between the cerebral cortices and the hypothalamus. Lipopolysaccharide and chronic social defeat stress, both involving the innate immune receptor TLR4, upregulated marker genes of selective microglial subpopulations. These findings highlight the contribution of microglial subpopulations to the heterogeneity of microglial transcriptome and responsiveness in different brain regions.
JOURNAL OF PHARMACOLOGICAL SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Walker D. Short, Emily Steen, Aditya Kaul, Xinyi Wang, Oluyinka O. Olutoye, Hima V. Vangapandu, Natalie Templeman, Alexander J. Blum, Chad M. Moles, Daria A. Narmoneva, Timothy M. Crombleholme, Manish J. Butte, Paul L. Bollyky, Sundeep G. Keswani, Swathi Balaji
Summary: This study found that overexpression of IL-10 in dermal wounds recruits EPCs, increases vascular structures, and accelerates re-epithelialization.
Article
Cell Biology
Eliane Sibler, Yuliang He, Luca Ducoli, Nadja Keller, Noriki Fujimoto, Lothar C. Dieterich, Michael Detmar
Summary: The study used single-cell RNA sequencing to investigate transcriptional changes in lymphatic endothelial cells in lymph nodes draining naive and inflamed skin. It was found that subsets of these cells responded individually to skin inflammation, suggesting distinct functions under pathological conditions. Dysregulated genes during inflammation were identified, including an up-regulation of CD200, potentially involved in immune regulation. Additionally, in silico analysis predicted interactions of these cells with diverse immune cells in the lymph nodes, showing similarities in transcriptional changes in different skin inflammation settings.
Review
Biology
Aidan S. Marshall, Nick S. Jones
Summary: Mitochondria, the powerhouse of the cell, exist in multiple copies in almost every cell, each with its own mtDNA. Mutations in mtDNA can lead to cell dysfunction and disease. Heteroplasmy, a state where cells harbor both wild type and variant mtDNA, can arise through inheritance or mutations throughout life.
Article
Gastroenterology & Hepatology
Tingting Su, Yilin Yang, Sanchuan Lai, Jain Jeong, Yirang Jung, Matthew McConnell, Teruo Utsumi, Yasuko Iwakiri
Summary: This study mapped the spatial distribution and transcriptomic changes of liver sinusoidal endothelial cells (LSECs) in normal vs cirrhotic mouse livers, providing insights into the pathogenesis of liver cirrhosis.
CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY
(2021)
Article
Cell Biology
Anthony Leyme, Arthur Marivin, Lorena Perez-Gutierrez, Lien T. Nguyen, Mikel Garcia-Marcos
JOURNAL OF CELL BIOLOGY
(2015)
Article
Immunology
Tamara Girbl, Tchern Lenn, Lorena Perez, Loic Rolas, Anna Barkaway, Aude Thiriot, Carlos del Fresno, Eleanor Lynam, Elin Hub, Marcus Thelen, Gerard Graham, Ronen Alon, David Sancho, Ulrich H. von Andrian, Mathieu-Benoit Voisin, Antal Rot, Sussan Nourshargh
Article
Immunology
Natalia Reglero-Real, Lorena Perez-Gutierrez, Azumi Yoshimura, Loic Rolas, Jose Garrido-Mesa, Anna Barkaway, Catherine Pickworth, Rebeca S. Saleeb, Maria Gonzalez-Nunez, Shani N. Austin-Williams, Dianne Cooper, Laura Vazquez-Martinez, Tao Fu, Giulia De Rossi, Matthew Golding, Mathieu-Benoit Voisin, Chantal M. Boulanger, Yoshiaki Kubota, William A. Muller, Sharon A. Tooze, Thomas D. Nightingale, Lucy Collinson, Mauro Perretti, Ezra Aksoy, Sussan Nourshargh
Summary: This study identified autophagy as a modulator of endothelial cell leukocyte trafficking machinery aimed at terminating physiological inflammation.
Editorial Material
Cell Biology
Natalia Reglero-Real, Lorena Perez-Gutierrez, Sussan Nourshargh
Summary: Inflammatory reactions are characterized by the infiltration of neutrophils into tissues, which is regulated by endothelial cell autophagy. Specifically, autophagy machinery controls the architecture of endothelial cell contacts and the reorganization and degradation of adhesion molecules, serving as a physiological brake on leukocyte trafficking.
Article
Medicine, Research & Experimental
Pin Li, Qin Li, Nilima Biswas, Hong Xin, Tanja Diemer, Lixian Liu, Lorena Perez Gutierrez, Giovanni Paternostro, Carlo Piermarocchi, Sergii Domanskyi, Ruikang K. Wang, Napoleone Ferrara
Summary: The study identified LIF as a potential therapeutic approach to prevent atrophy associated with AMD by protecting the choroid, highlighting its dual role as a mitogen for specific endothelial cells while inhibiting growth in others.
EMBO MOLECULAR MEDICINE
(2022)
Review
Cell Biology
Lorena Perez-Gutierrez, Napoleone Ferrara
Summary: The formation of new blood vessels, known as angiogenesis, is regulated by various factors, with vascular endothelial growth factor A (VEGFA) and its receptors VEGFR1 and VEGFR2 being key components. The VEGF pathway is the main target of anti-angiogenic drugs approved by the FDA, which are widely used in oncology and the treatment of neovascular eye disorders. In this Review, the molecular basis of VEGFA action, its interactions with other pathways, and its role in regenerative medicine are discussed.
NATURE REVIEWS MOLECULAR CELL BIOLOGY
(2023)