期刊
JOURNAL OF FOOD SCIENCE
卷 81, 期 3, 页码 H786-H793出版社
WILEY-BLACKWELL
DOI: 10.1111/1750-3841.13226
关键词
barley beta-glucans; 3T3-L1 cells; PPAR gamma; C/EBP alpha
资金
- Taiwan Science and Technology Cooperation Program of China [2013DFH30050]
- Special Fund for Agro-scientific Research in the Public Interest [201403063]
- Earmarked Fund for China Agriculture Research System Modern Agro-313 industry Technology [CYTX-014]
- Agricultural Science and Technology Program for Innovation Team on Identification and excavation of Elite Crop Germplasm, CAAS
In this study, 2 beta-glucans with different molecular weight were prepared and purified from hull-less barley bran. The aim was to evaluate their effects on the differentiation of 3T3-L1 pre-adipocytes. Results showed that barley beta-glucans inhibited the differentiation of 3T3-L1 pre-adipocytes induced by differentiation medium in a dose-dependent manner, the suppressive effect of high-molecular-weight barley beta-glucans (552 kDa, BGH) was stronger (P < 0.05) than that of low-molecular-weight barley beta-glucan (32 kDa, BGL), evidenced by the significantly decrease (P < 0.05) of Oil-red O staining and intracellular triglyceride content in the mature adipocytes. Besides, gene expression analysis and Western Blot analysis revealed that both BGH and BGL inhibited the mRNA and protein levels of adipogenesis related transcription factors peroxisome proliferator-activated receptor. (PPAR.) and CCAAT-enhancer-binding protein a (C/EBP alpha) which are principal regulators of adipogenesis. Furthermore, the mRNA and protein expression levels of PPAR. target genes in adipose tissue including adipocyte fatty acid binding protein (ap2), lipoprotein lipase (LPL), uncoupling protein-2 (UCP-2), and glucose-transporter 4 (Glut4) in 3T3-L1 cells was also markedly downregulated (P < 0.05). These findings were anticipated to help develop barley beta-glucans based functional food for the management of obesity.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据