Article
Biology
Jie Xiong, Zhaoyue Li, Hao Tang, Yuchen Duan, Xiaofang Ban, Ke Xu, Yutong Guo, Yingfeng Tu
Summary: This study aimed to investigate the heterogeneity of immune cells involved in atherosclerosis progression and poor prognosis by integrating sequencing results and using an enhanced single-cell sequencing workflow. The analysis identified 28 distinct subpopulations based on gene expression profiles, which showed high heterogeneity in tissue preferences, genetic perturbations, functional variations, immune dynamics, transcriptional regulators, metabolic changes, and communication patterns. Importantly, specific T cell subtypes and foamy macrophages were found to play significant roles in atherosclerosis progression and prognosis, providing potential targets for precise immunotherapies.
Article
Pharmacology & Pharmacy
Yan Wang, Di Qin, Yiyao Gao, Yunxin Zhang, Yao Liu, Lihong Huang
Summary: This study used single-cell RNA sequencing and network pharmacology to analyze effective targets for Osteosarcoma treatment. The cell heterogeneity of the Osteosarcoma single-cell dataset was analyzed, and key targets for OS therapy were determined. GO and KEGG analysis showed that the identified genes were associated with immune regulation pathways. Molecular docking analysis supported the potential drug-target pair for Osteosarcoma treatment.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Cell & Tissue Engineering
Naoki Ishiuchi, Ayumu Nakashima, Satoshi Maeda, Yoshie Miura, Kisho Miyasako, Kensuke Sasaki, Toshio Uchiki, Ayano Sasaki, Shogo Nagamatsu, Naoki Nakao, Masataka Nagao, Takao Masaki
Summary: Fibrosis is a common histological feature in chronic organ injury, leading to organ failure. Adipose-derived mesenchymal stem cells (ASCs) have been studied as a therapeutic tool to prevent organ failure. This study compared the therapeutic effects of ASCs from superficial and deep layers and found that both types of ASCs are equally effective and safe for treating organ injury.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Transplantation
Snigdha N. Rao, Margot Zahm, Audrey Casemayou, Marie Buleon, Stanislas Faguer, Guylene Feuillet, Jason S. Iacovoni, Olivier P. Joffre, Ignacio Gonzalez-Fuentes, Emeline Lhuillier, Frederic Martins, Elodie Riant, Alexia Zakaroff-Girard, Joost P. Schanstra, Jean Sebastien Saulnier-Blache, Julie Belliere
Summary: Single-cell RNA sequencing revealed a novel macrophage subpopulation associated with rhabdomyolysis-induced acute kidney injury (RM-AKI) that is characterized by cellular senescence. This study provides proof-of-concept that senolytics could have nephroprotective effects through immune modulation.
NEPHROLOGY DIALYSIS TRANSPLANTATION
(2023)
Review
Geriatrics & Gerontology
Min Qiu, Jia-bin Zong, Quan-wei He, Yu-xiao Liu, Yan Wan, Man Li, Yi-fan Zhou, Jie-hong Wu, Bo Hu
Summary: This review summarizes the key genes in brain cells and specific pathways revealed by single-cell RNA sequencing, providing valuable insights into the pathological mechanisms of ischemic stroke and the development of new treatment strategies.
Article
Genetics & Heredity
Jianjun Huang, Li Liu, Lingling Qin, Hehua Huang, Xue Li
Summary: This study utilized single-cell transcriptomics to reveal cellular heterogeneity and identified the importance of endothelial cells in Parkinson's disease (PD). Five upregulated marker genes of endothelial cells were identified as potential therapeutic targets for PD.
FRONTIERS IN GENETICS
(2022)
Article
Cell Biology
Ganbin Li, Haoyu Zhang, Ke Cao, Zhiwei Zhai, Zhenjun Wang, Lei Yang, Jiagang Han
Summary: This study used whole transcriptome RNA sequencing to analyze the differential expression of noncoding RNA (ncRNA) and mRNA in visceral adipose tissue from obese and lean individuals. The results revealed the involvement of ncRNAs in inflammation-related pathways and identified an obesity-associated competing endogenous RNA (ceRNA) network regulated by circORC5 and circNTRK2.
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2022)
Article
Pharmacology & Pharmacy
Andres Lopez-Cortes, Patricia Guevara-Ramirez, Nikolaos C. Kyriakidis, Carlos Barba-Ostria, Angela Leon Caceres, Santiago Guerrero, Esteban Ortiz-Prado, Cristian R. Munteanu, Eduardo Tejera, Domenica Cevallos-Robalino, Ana Maria Gomez-Jaramillo, Katherine Simbana-Rivera, Adriana Granizo-Martinez, Gabriela Perez-M, Silvana Moreno, Jennyfer M. Garcia-Cardenas, Ana Karina Zambrano, Yunierkis Perez-Castillo, Alejandro Cabrera-Andrade, Lourdes Puig San Andres, Carolina Proano-Castro, Jhommara Bautista, Andreina Quevedo, Nelson Varela, Luis Abel Quinones, Cesar Paz-y-Mino
Summary: Potential therapeutic targets for drug repurposing against COVID-19 were identified through in silico analyses of the immune system protein interactome network, single-cell RNA sequencing of human tissues, and artificial neural networks. These targets, significantly overexpressed in specific cells such as nasal, lung, and ileal cells, can be considered for treatment of severe COVID-19 patients after effective clinical trial analysis.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Immunology
Andrew D. Hildreth, Feiyang Ma, Yung Yu Wong, Ryan Sun, Matteo Pellegrini, Timothy E. O'Sullivan
Summary: This study provides a comprehensive analysis of the cellular composition in healthy and obese human white adipose tissue, revealing changes in immune cell populations and inflammatory pathways in obese individuals. The findings highlight the importance of immune cell regulation in metabolic diseases and offer insights into new cellular networks and differentiation trajectories in white adipose tissue.
Review
Oncology
Boyu Sun, Ziyu Xun, Nan Zhang, Kai Liu, Xiangqi Chen, Haitao Zhao
Summary: Immune checkpoint blockade is a promising strategy in treating advanced cancers, but only a small fraction of patients benefit from it. The tumor microenvironment plays a crucial role in the efficacy of immune checkpoint blockade, and single-cell RNA sequencing helps to understand its complex composition, study the mechanisms behind the treatment response, and discover biomarkers for predicting response and therapeutic targets.
CANCER CELL INTERNATIONAL
(2023)
Article
Biochemistry & Molecular Biology
Rashid Ahmed, Tariq Zaman, Farhan Chowdhury, Fatima Mraiche, Muhammad Tariq, Irfan S. Ahmad, Anwarul Hasan
Summary: Single-cell RNA sequencing techniques have the potential to explore the development and progression of tumors by analyzing the transcriptome at the single-cell level. These techniques provide a better understanding of the cellular diversity and interactions in the tumor microenvironment. Integrating single-cell RNA sequencing with other omics technologies revolutionizes the understanding of tumor microenvironment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Cankun Zhou, Minqing Feng, Yonglian Chen, Side Lv, Yifan Zhang, Jiebo Chen, Rujian Zhang, Xiaobin Huang
Summary: This study identified potential immune-related biomarkers for the diagnosis and treatment of endometriosis, providing valuable insights for developing clinically relevant strategies. Abnormal immune cell distribution, particularly increased macrophages, was observed in endometriosis, indicating the potential of immune genes CXCL12, ROBO3, and SCG2 as drug targets.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Medicine, General & Internal
Tim J. Enz, James R. Tribble, Pete A. Williams
Summary: The study identified shared differentially expressed genes in different glaucoma models and screened compounds that target these genes for potential neuroprotective therapies. Experimental validation showed that folic acid, valproic acid, and WY-14643 demonstrated significant protection against retinal ganglion cell death. The increasing availability of open access-omics data serves as a valuable resource for future therapeutic investigations.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Genetics & Heredity
Niambi Brewer, John T. Fong, Deyu Zhang, Girish Ramaswamy, Eileen M. Shore
Summary: Progressive osseous heteroplasia (POH) is caused by inactivation of the GNAS gene, resulting in heterotopic bone formation starting in subcutaneous tissues and progressing to deeper connective tissues. Gnas inactivation leads to depletion of adipose tissue and increased extracellular matrix density, promoting heterotopic bone formation. The tissue microenvironment influenced by Gnas inactivation affects mutant and wild-type progenitor cells, contributing to heterotopic ossification (HO) formation.
FRONTIERS IN GENETICS
(2021)
Article
Pathology
Junyan Tao, Dantong Sun, Hai Zhou, Jingjuan Zhu, Xiaochun Zhang, Helei Hou
Summary: The study showed that 39.7% of HGSOC patients harbored at least one actionable genetic alteration, with 25.0% of patients having mutations beyond BRCA1 that may require off-label therapy or participation in clinical trials. NGS assays are necessary to screen actionable genetic alterations in HGSOC patients for personalized and precise treatment.
PATHOLOGY RESEARCH AND PRACTICE
(2022)