Microscale thermophoresis as a powerful growing analytical technique for the investigation of biomolecular interaction and the determination of binding parameters

The Acid-Base/Deprotonation Equilibrium Can Be Studied with a MicroScale Thermophoresis (MST)

(2022) Paweł Mateusz Nowak et al.   MOLECULES

Evaluation of Xa inhibitors as potential inhibitors of the SARS-CoV-2 Mpro protease

(2022) Katarzyna Papaj et al.   PLoS One

Novel diagnostic and prognostic factors for the advanced melanoma based on the glycosylation-related changes studied by biophysical profiling methods

(2022) Anna Sobiepanek et al.   BIOSENSORS & BIOELECTRONICS

Cost-effective, green HPLC determination of losartan, valsartan and their nitrosodiethylamine impurity: application to pharmaceutical dosage forms

(2022) Soad S. Abd El-Hay et al.   Royal Society Open Science

Analytical Performance and Greenness Evaluation of Five Multi-Level Design Models Utilized for Impurity Profiling of Favipiravir, a Promising COVID-19 Antiviral Drug

(2022) Adel Ehab Ibrahim et al.   MOLECULES

Exploring the Unique Contrast Properties of Aptamer–Gadolinium Conjugates in Magnetic Resonance Imaging for Targeted Imaging of Thrombi

(2021) Anna Koudrina et al.   ACS Applied Materials & Interfaces

Aptamers against the β-Conglutin Allergen: Insights into the Behavior of the Shortest Multimeric (Intra)Molecular DNA G-Quadruplex

(2021) Ciara K. O’ Sullivan et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Human DDX3X Unwinds Japanese Encephalitis and Zika Viral 5′ Terminal Regions

(2021) Corey Nelson et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Biochemical and structural analyses reveal critical residues in δ subunit affecting its bindings to β′ subunit of Staphylococcus aureus RNA polymerase

(2021) Zhaozhu Lin et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Spontaneous and frequent conformational dynamics induced by A…A mismatch in d(CAA)·d(TAG) duplex

(2021) Yogeeshwar Ajjugal et al.   Scientific Reports

Multifaceted Mechanism of Amicoumacin A Inhibition of Bacterial Translation

(2021) Elena M. Maksimova et al.   Frontiers in Microbiology

Simultaneous determination of zearalenone and ochratoxin A based on microscale thermophoresis assay with a bifunctional aptamer

(2021) Yan Yang et al.   ANALYTICA CHIMICA ACTA

Standard operation procedure for switchSENSE DRX systems

(2021) Hanna Müller-Landau et al.   EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS

Microscale thermophoresis suggests a new model of regulation of cardiac myosin function via interaction with cardiac myosin-binding protein C

(2021) Saraswathi Ponnam et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Design, Synthesis, and Anti-ToCV Activity of Novel 4(3H)-Quinazolinone Derivatives Bearing Dithioacetal Moiety

(2020) Guangcheng Zu et al.   JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

Mitigating effects of osmolytes on the interactions between nanoparticles and supported lipid bilayer

(2020) Zehui Xia et al.   JOURNAL OF COLLOID AND INTERFACE SCIENCE

Membrane Phospholipids and Polyphosphates as Cofactors and Binding Molecules of SERPINA12 (vaspin)

(2020) Catherine A. Tindall et al.   MOLECULES

Chimeric Peptidomimetics of SOCS 3 Able to Interact with JAK2 as Anti-inflammatory Compounds

(2020) Sara La Manna et al.   ACS Medicinal Chemistry Letters

Mycobacterium tuberculosis ribosomal protein S1 (RpsA) and variants with truncated C-terminal end show absence of interaction with pyrazinoic acid

(2020) Katherine Vallejos-Sánchez et al.   Scientific Reports

Metallacarborane Complex Boosts the Rate of DNA Oligonucleotide Hydrolysis in the Reaction Catalyzed by Snake Venom Phosphodiesterase

(2020) Damian Kaniowski et al.   Biomolecules

Exploring IDP–Ligand Interactions: Tau K18 as a Test Case

(2020) Darius Vagrys et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Effects of gold nanoparticle morphologies on interactions with proteins

(2020) Gongke Wang et al.   Materials Science & Engineering C-Materials for Biological Applications

Assessment of binding interaction dihydromyricetin and myricetin with bovine lactoferrin and effects on antioxidant activity

(2020) Junyi Huang et al.   SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY

Structure of the Human Respiratory Syncytial Virus M2-1 Protein in Complex with a Short Positive-Sense Gene-End RNA

(2020) Yunrong Gao et al.   STRUCTURE

How to measure and evaluate binding affinities

(2020) Inga Jarmoskaite et al.   eLife

Microscale Thermophoresis as a Screening Tool to Predict Melanin Binding of Drugs

(2020) Laura Hellinen et al.   Pharmaceutics

New insights on the interaction mechanism of rhTNFα with its antagonists Adalimumab and Etanercept

(2020) María Angélica Contreras et al.   BIOCHEMICAL JOURNAL

Investigation of the enantioselective interaction between selected drug enantiomers and human serum albumin by mobility shift‐affinity capillary electrophoresis

(2020) Ratih Ratih et al.   JOURNAL OF SEPARATION SCIENCE

Blood-Brain Barrier Permeable Chitosan Oligosaccharides Interfere with β-Amyloid Aggregation and Alleviate β-Amyloid Protein Mediated Neurotoxicity and Neuroinflammation in a Dose- and Degree of Polymerization-Dependent Manner

(2020) Limeng Zhu et al.   Marine Drugs

Odorant binding protein 2 reduces imidacloprid susceptibility of Diaphorina citri

(2020) Xiao-Qiang Liu et al.   PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY

Modulation of inner filter effect of non-conjugated silver nanoparticles on blue emitting ZnS quantum dots for the quantitation of betahistine

(2020) Vijay D. Chavada et al.   SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY

Human Serum Albumin Binds Native Insulin and Aggregable Insulin Fragments and Inhibits Their Aggregation

(2020) Joanna Wasko et al.   Biomolecules

On the correlation of cereblon binding, fluorination and antiangiogenic properties of immunomodulatory drugs

(2020) Christopher Heim et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Human DDX17 Unwinds Rift Valley Fever Virus Non-Coding RNAs

(2020) Corey R. Nelson et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Repurposing of genistein as anti-sickling agent: elucidation by multi spectroscopic, thermophoresis, and molecular modeling techniques

(2020) Muntjeeb M. Syed et al.   JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS

The effects of L-arginine on protein stability and DNA binding ability of SaeR, a transcription factor in Staphylococcus aureus

(2020) Ruochen Fan et al.   PROTEIN EXPRESSION AND PURIFICATION

An In-Silico Pipeline for Rapid Screening of DNA Aptamers against Mycotoxins: The Case-Study of Fumonisin B1, Aflatoxin B1 and Ochratoxin A

(2020) Fulvio Ciriaco et al.   Polymers

Strong interactions between Salp15 homologues from the tick I. ricinus and distinct types of the outer surface OspC protein from Borrelia

(2020) Paulina Bierwagen et al.   Ticks and Tick-Borne Diseases

Structural insights into BCL2 pro-survival protein interactions with the key autophagy regulator BECN1 following phosphorylation by STK4/MST1

(2019) Erinna F. Lee et al.   Autophagy

MCC1019, a selective inhibitor of the Polo-box domain of Polo-like kinase 1 as novel, potent anticancer candidate

(2019) Sara Abdelfatah et al.   Acta Pharmaceutica Sinica B

A mutually-induced conformational fit underlies Ca2+-directed interactions between calmodulin and the proximal C terminus of KCNQ4 K+ channels

(2019) Crystal R. Archer et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Purification and Characterization of a Secretory Alkaline Metalloprotease with Highly Potent Antiviral Activity from Serratia marcescens Strain S3

(2019) Yuanxia Qin et al.   JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

Integron Derived Aminoglycoside-Sensing Riboswitches Control Aminoglycoside Acetyltransferase Resistance Gene Expression

(2019) Shasha Wang et al.   ANTIMICROBIAL AGENTS AND CHEMOTHERAPY

Comparison of different label-free imaging high-throughput biosensing systems for aptamer binding measurements using thrombin aptamers

(2019) Christin Rath et al.   ANALYTICAL BIOCHEMISTRY

N1-Propargylguanosine Modified mRNA Cap Analogs: Synthesis, Reactivity, and Applications to the Study of Cap-Binding Proteins

(2019) Michal Kopcial et al.   MOLECULES

Rational drug-design approach supported with thermodynamic studies — a peptide leader for the efficient bi-substrate inhibitor of protein kinase CK2

(2019) Maria Winiewska-Szajewska et al.   Scientific Reports

GS-CA Compounds: First-In-Class HIV-1 Capsid Inhibitors Covering Multiple Grounds

(2019) Kamal Singh et al.   Frontiers in Microbiology

Studying the Potassium-Induced G-Quadruplex DNA Folding Process Using Microscale Thermophoresis

(2019) Ming-Li Zhang et al.   BIOCHEMISTRY

Aspulvinone O, a natural inhibitor of GOT1 suppresses pancreatic ductal adenocarcinoma cells growth by interfering glutamine metabolism

(2019) Weiguang Sun et al.   Cell Communication and Signaling

Novel colloidal associations of soyasaponins and lipid components (DPPC, cholesterol) as potential adjuvants for vaccines

(2019) Carolin de Groot et al.   VACCINE

Molecular Analysis of Protein-Protein Interactions in the Ethylene Pathway in the Different Ethylene Receptor Subfamilies

(2019) Mareike Berleth et al.   Frontiers in Plant Science

Structure-activity relationships for binding of 4-substituted triazole-phenols to macrophage migration inhibitory factor (MIF)

(2019) Zhangping Xiao et al.   EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY

Competitive Interactions between PIRT, the Cold Sensing Ion Channel TRPM8, and PIP2 Suggest a Mechanism for Regulation

(2019) Nicholas J. Sisco et al.   Scientific Reports

Computational design and characterization of nanobody-derived peptides that stabilize the active conformation of the β2-adrenergic receptor (β2-AR)

(2019) Milan Sencanski et al.   Scientific Reports

Tuning PFKFB3 Bisphosphatase Activity Through Allosteric Interference

(2019) Helena Macut et al.   Scientific Reports

Studying Interactions between 2’-O-Me-Modified Inhibitors and MicroRNAs Utilizing Microscale Thermophoresis

(2019) Markus Herkt et al.   Molecular Therapy-Nucleic Acids

Analytical approaches for the determination of deferiprone and its iron (III) complex: Investigation of binding affinity based on liquid chromatography-mass spectrometry (LC-ESI/MS) and capillary electrophoresis-frontal analysis (CE/FA)

(2019) Mufarreh Asmari et al.   MICROCHEMICAL JOURNAL

A rapid solution-based method for determining the affinity of heroin hapten-induced antibodies to heroin, its metabolites, and other opioids

(2018) Oscar B. Torres et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Using two-site binding models to analyze microscale thermophoresis data

(2018) Shih-Chia Tso et al.   ANALYTICAL BIOCHEMISTRY

Genetic coding algorithm for sense and antisense peptide interactions

(2018) Nikola Štambuk et al.   BIOSYSTEMS

Protein–protein interactions in AQP regulation – biophysical characterization of AQP0–CaM and AQP2–LIP5 complex formation

(2018) Stefan Kreida et al.   FARADAY DISCUSSIONS

Analysis of the pH-Dependent Fe(III) Ion Chelating Activity of Anthocyanin Extracted from Black Soybean [Glycine max (L.) Merr.] Coats

(2018) Yanli Xie et al.   JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

Thermophoresis for characterizing biomolecular interaction

(2018) Mufarreh Asmari et al.   METHODS

Near-native, site-specific and purification-free protein labeling for quantitative protein interaction analysis by MicroScale Thermophoresis

(2018) Tanja Bartoschik et al.   Scientific Reports

Protein–protein interactions in AQP regulation – biophysical characterization of AQP0–CaM and AQP2–LIP5 complex formation

(2018) Stefan Kreida et al.   FARADAY DISCUSSIONS

Using Microscale Thermophoresis to Characterize Hits from High-Throughput Screening: A European Lead Factory Perspective

(2018) Julie M. Rainard et al.   SLAS Discovery

A Chemical Biology Approach to Model Pontocerebellar Hypoplasia Type 1B (PCH1B)

(2018) Liberty François-Moutal et al.   ACS Chemical Biology

Probing ATP/ATP-Aptamer or ATP-Aptamer Mutant Complexes by Microscale Thermophoresis and Molecular Dynamics Simulations: Discovery of an ATP-Aptamer Sequence of Superior Binding Properties

(2018) Yonatan Biniuri et al.   JOURNAL OF PHYSICAL CHEMISTRY B

Assessment of Binding Interaction between Bovine Lactoferrin and Tetracycline Hydrochloride: Multi-Spectroscopic Analyses and Molecular Modeling

(2018) Qifan Sun et al.   MOLECULES

Virtual Screening and Biomolecular Interactions of CviR-Based Quorum Sensing Inhibitors Against Chromobacterium violaceum

(2018) Vinothkannan Ravichandran et al.   Frontiers in Cellular and Infection Microbiology

Human αS1-casein induces IL-8 secretion by binding to the ecto-domain of the TLR4/MD2 receptor complex

(2018) Thorsten Saenger et al.   BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS

The characterization and validation of 17β-estradiol binding aptamers

(2017) Markéta Svobodová et al.   JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

(2017) Clemens Entzian et al.   Jove-Journal of Visualized Experiments

ITC-derived binding affinity may be biased due to titrant (nano)-aggregation. Binding of halogenated benzotriazoles to the catalytic domain of human protein kinase CK2

(2017) Maria Winiewska et al.   PLoS One

Exploring the binding of d(GGGT)4 to the HIV-1 integrase: An approach to investigate G-quadruplex aptamer/target protein interactions

(2016) Veronica Esposito et al.   BIOCHIMIE

Structure and target interaction of a G-quadruplex RNA-aptamer

(2016) Kristina Szameit et al.   RNA Biology

Screening and Identification of DNA Aptamers to Tyramine Using in Vitro Selection and High-Throughput Sequencing

(2016) Stefania Valenzano et al.   ACS Combinatorial Science

Comparison of In-Solution Biorecognition Properties of Aptamers against Ochratoxin A

(2016) Maureen McKeague et al.   Toxins

Tunable stringency aptamer selection and gold nanoparticle assay for detection of cortisol

(2014) Jennifer A. Martin et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Novel microscale approaches for easy, rapid determination of protein stability in academic and commercial settings

(2014) Crispin G. Alexander et al.   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

MicroScale Thermophoresis: Interaction analysis and beyond

(2014) Moran Jerabek-Willemsen et al.   JOURNAL OF MOLECULAR STRUCTURE

Metrology for metalloproteins—where are we now, where are we heading?

(2013) Claudia Swart   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Thermodynamic origins of protein folding, allostery, and capsid formation in the human hepatitis B virus core protein

(2013) C. G. Alexander et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Label-Free Microscale Thermophoresis Discriminates Sites and Affinity of Protein-Ligand Binding

(2012) Susanne A. I. Seidel et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Microscale thermophoresis quantifies biomolecular interactions under previously challenging conditions

(2012) Susanne A.I. Seidel et al.   METHODS

Molecular Interaction Studies Using Microscale Thermophoresis

(2011) Moran Jerabek-Willemsen et al.   ASSAY AND DRUG DEVELOPMENT TECHNOLOGIES

Protein-binding assays in biological liquids using microscale thermophoresis

(2010) Christoph J. Wienken et al.   Nature Communications