Article
Plant Sciences
Han Jiang, Qi-Jun Ma, Ming-Shuang Zhong, Huai-Na Gao, Yuan-Yuan Li, Yu-Jin Hao
Summary: In this study, MdPAT16 was identified as a key player in mediating salt stress response in apple plants through palmitoylation of target proteins. Overexpression of MdPAT16 improved plant salt stress resistance and promoted the accumulation of soluble sugars. Furthermore, MdPAT16 was shown to interact with MdCBL1 and stabilize its protein levels through palmitoylation, suggesting a potential missing link in the plant salt stress response pathway.
Article
Food Science & Technology
Alexandra Buergy, Agnes Rolland-Sabate, Alexandre Leca, Catherine M. G. C. Renard
Summary: The impact of apple cultivars and processing methods on the texture of purees was investigated in this study. Viscosity of purees varied significantly between different cultivars, with Granny Smith cultivar showing the highest viscosity due to its firm fruits and large particles. Mealy Braeburn apples had lower viscosity purees compared to non-mealy ones, attributed to reduced cell adhesion. Processing method also affected the viscosity of purees, with high temperature-low shear process resulting in more viscous purees.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Plant Sciences
Xingqiang Fan, Yuqi Li, Cecilia H. H. Deng, Shiyao Wang, Zijun Wang, Yi Wang, Changpeng Qiu, Xuefeng Xu, Zhenhai Han, Wei Li
Summary: Propagation through stem cuttings is a popular method for clonal propagation of certain tree species. This study investigates the regulatory mechanisms of adventitious root formation in apple trees. The research identifies the gene MdBRC1 as a key player in inhibiting adventitious root formation. The study also proposes potential target genes and the use of CRISPR technology to enhance adventitious root formation in woody plants.
Article
Plant Sciences
Simon Miranda, Stefano Piazza, Floriana Nuzzo, Mingai Li, Jorge Lagreze, Axel Mithoefer, Alessandro Cestaro, Danuse Tarkowska, Richard Espley, Andrew Dare, Mickael Malnoy, Stefan Martens
Summary: The effects of targeting the key gene MdPGT1 involved in phloridzin biosynthesis in apple using conventional transgenesis and CRISPR/Cas9-mediated genome editing were simultaneously assessed. Knockdown lines showed impaired growth and leaf morphology, while genome-edited lines exhibited normal growth despite reduced phloridzin levels. Transcriptomic and metabolic analyses revealed regulated genes involved in phenylpropanoid and flavonoid pathways. Phytohormone profiling demonstrated differential modulation, with increased salicylic and jasmonic acid levels in dwarf lines and no correlation between auxin and ABA with growth phenotype.
Article
Biochemistry & Molecular Biology
Yifeng Feng, Shanshan Li, Rongjian Jia, Jie Yang, Qiufang Su, Zhengyang Zhao
Summary: Exposure to high-intensity sunlight can lead to sunburn on fruits, causing damage to peel cell structure and color changes. Fruits initially try to enhance photosynthetic protection ability and antioxidant enzyme activity in response to external injury.
Article
Food Science & Technology
Caiping Huang, Bangzhu Peng
Summary: A high-performance nitrogen-doped chitosan-TiO2 nanocomposite was successfully prepared by a simple precipitation method and showed significant photocatalytic activities for the degradation of patulin in apple juice. The nanocomposite effectively degraded patulin within 35 minutes under ultraviolet irradiation, with little impact on the quality of apple juice.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Plant Sciences
Hui Wang, Shuang Wang, Miao-Miao Fan, Shu-Hui Zhang, Lu-Long Sun, Zheng-Yang Zhao
Summary: Bagging is an important technique for producing high-quality fruits, but it can lead to browning in apple peel due to changes in microenvironment, destruction of cell structure, decrease in flavonoids, and increase in triterpenoids.
Article
Medicine, Research & Experimental
Mona F. Mahmoud, Walied Abdo, Mohamed Nabil, Badreddine Drissi, Assem M. El-Shazly, Mohamed A. O. Abdelfattah, Mansour Sobeh
Summary: The butanol fraction of apple leaves (BLE) exhibited gastro-protective and healing effects against indomethacin-induced gastric ulcers in rats. It reduced oxidative stress, inflammatory cytokines, and COX-2 expression and increased mucoglycoproteins and HSP70 expression. These effects are likely due to the presence of phytoconstituents.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Food Science & Technology
Weijie Lan, Sylvie Bureau, Songchao Chen, Alexandre Leca, Catherine M. G. C. Renard, Benoit Jaillais
Summary: The study successfully applied different spectroscopic techniques and modeling methods to trace the proportions of apple varieties in purees and predict the quality characteristics. The PLS models performed well in estimating the proportions of apple cultivars in mixtures, while MIRS technique was effective in predicting the quality of final purees based on spectral data of composed cultivars.
Article
Agronomy
Md Shipon Miah, Chloe Hinson, Macarena Farcuh
Summary: This research evaluated the horticultural performance of 'Buckeye Gala' apple scion grafted onto different rootstocks. The results showed that different rootstocks have significant impacts on tree size, yield, and fruit maturity. Considering the importance of rootstocks in fruit maturity and quality, it is crucial to take them into account when making management decisions in Western Maryland orchards.
Article
Plant Sciences
Stefan Roeder, Sara Serra, Stefano Musacchi
Summary: In this study, apple cultivars were categorized based on their pollen tube growth performance, with 41 individuals divided into three subgroups using statistical analysis and a decision tree model for prediction. Thresholds were established from the decision tree to characterize new observations, demonstrating the effectiveness of the methods in a case study with 29 apple accessions.
Article
Horticulture
Leo Omar Duarte Marques, Mateus da Silveira Pasa, Paulo Mello-Farias, Flavio Gilberto Herter
Summary: This study evaluates the efficiency of different budburst inducers for apple trees and finds that garlic extract is not effective, but hydrogen cyanamide and Erger (R) are good alternatives. The biological method of single bud is not suitable for evaluating the efficiency of budburst inducers.
SCIENTIA HORTICULTURAE
(2022)
Article
Horticulture
S. Alessandri, R. Gregori, L. Dondini, S. Sansavini
Summary: A molecular characterization was conducted on the ancient apple variety 'Rosa Romana' to identify genotype groups and their relationship with phenotypic traits. The study revealed higher quality traits of 'Rosa Romana' apples grown in the Apennines mountain compared to those produced at lower altitudes. The genomic validation of identified clones allows for the recovery and promotion of new plantings of 'Rosa Romana' apples in the Apennines mountain region of Italy.
SCIENTIA HORTICULTURAE
(2021)
Article
Microbiology
Felix Mahnkopp-Dirks, Viviane Radl, Susanne Kublik, Silvia Gschwendtner, Michael Schloter, Traud Winkelmann
Summary: Apple Replant Disease (ARD) is a widespread problem in the horticultural industry, affecting plant growth and fruit quality. This study used both culture-independent and culture-dependent approaches to investigate the endophytic root microbiome of apple plants in ARD-affected and unaffected soil. The results suggest that Streptomyces play a key role in the etiology of ARD in the field.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yongzhou Li, Xu Tan, Jing Guo, Enyue Hu, Qi Pan, Yuan Zhao, Yu Chu, Yuandi Zhu
Summary: The TAC1 gene family plays a crucial role in controlling plant branch angle and plant form. This study identified the subcellular localization of MdTAC1a and MdTAC1b in apple, showing their importance in regulating leaf angles and plant morphology. The interaction between MdTAC1a and MdSRC2, highly expressed in trees with large branch angles, may be a key factor influencing branch-angle variation in apple.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Maria Dolores Camalle, Ales Pencik, Ondrej Novak, Lina Zhao, Udi Zurgil, Aaron Fait, Noemi Tel-Zur
Summary: The collapse of certain melon-pumpkin graft combinations may be caused by impairment of photoassimilates transport and imbalance of root auxin and cytokinins. Incompatible combinations show elevated levels of IAA and certain cytokinins in the roots during fruit ripening, while restricting transport of photoassimilates and inducing their accumulation in the scion.
HORTICULTURE RESEARCH
(2022)
Article
Plant Sciences
Klaus-Peter Goetz, Frank-M Chmielewski, Danuse Tarkowska, Ales Pencik, Ondrej Novak
Summary: This study was conducted to assess the involvement of gibberellins (GAs), cytokinins (CKs), and auxins (AX) in the dormancy of sweet cherry buds. The results showed that these hormone groups did not exhibit significant changes during the transition between different dormancy phases. However, there were specific changes in the content of bioactive molecules during ontogenetic development.
JOURNAL OF PLANT GROWTH REGULATION
(2023)
Article
Multidisciplinary Sciences
Lanxin Li, Huihuang Chen, Saqer S. Alotaibi, Ales Pencik, Maciek Adamowski, Ondrej Novak, Jiri Friml
Summary: This study investigates the mechanism of rapid alkalinization factors (RALFs) in regulating plant growth. It is found that RALF1 inhibits the growth of Arabidopsis thaliana roots by inducing apoplast alkalinization, which is mediated by the receptor FERONIA (FER) through net H+ influx. Additionally, the study reveals cross-talk between RALF1 and auxin signaling pathways, where RALF1 up-regulates auxin biosynthesis to regulate root growth. The biphasic action of RALF1 on growth allows plants to respond rapidly to environmental stimuli and reprogram growth and development in the long term.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Plant Sciences
Ioanna Antoniadi, Eduardo Mateo-Bonmati, Marketa Pernisova, Federica Brunoni, Mariana Antoniadi, Mauricio Garcia-Atance Villalonga, Anita Ament, Michal Karady, Colin Turnbull, Karel Dolezal, Ales Pencik, Karin Ljung, Ondrej Novak
Summary: Cytokinin and auxin are plant hormones that coordinate plant development. Their interactions in plant underground growth are well established. Different types of cytokinins have different effects on root growth, while some mutants show root phenotypes opposite to known cytokinin functions and related to auxin levels.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemical Research Methods
Jitka Siroka, Federica Brunoni, Ales Pencik, Vaclav Mik, Asta Zukauskaite, Miroslav Strnad, Ondrej Novak, Kristyna Flokova
Summary: This study optimized a high-throughput sample preparation method for liquid chromatography-tandem mass spectrometry determination of acidic phytohormones. The method allows analysis of minute amounts of plant material and is suitable for large-scale interspecies studies.
Article
Biochemistry & Molecular Biology
Milica Milutinovic, Dura Nakarada, Jelena Bozunovic, Milos Todorovic, Uros Gasic, Suzana Zivkovic, Marijana Skoric, Durda Ivkovic, Jelena Savic, Nina Devrnja, Neda Anicic, Tijana Banjanac, Milos Mojovic, Danijela Misic
Summary: This study provides a characterization of the redox processes associated with the ripening of bittersweet berries for the first time. Reactive oxygen species were measured using Electron Paramagnetic Resonance Spectroscopy and Imaging, and the metabolic profiling of antioxidants and antioxidant enzyme activity was assessed. Changes in antioxidants and oxidation processes occurred during the transition from green to ripe stage. The skin of bittersweet berries had the highest antioxidant potential, and the enzymatic antioxidant system played a critical protective role. The EPRI methodology was presented to assess the redox status of fruits and discriminate between different tissues. The intracellular reoxidation of nitroxide probe 3CP was observed for the first time and proposed as an indicator of oxidative stress during ripening. The noninvasive EPRI technique has the potential for broader application in studying redox processes in fruits and other oxidative stress-related circumstances.
Letter
Plant Sciences
Federica Brunoni, Ales Pencik, Asta Zukauskaite, Anita Ament, Martina Kopecna, Silvio Collani, David Kopecny, Ondrej Novak
Article
Horticulture
Nevena Banjac, Dijana Krstic-Milosevic, Tatjana Mijalkovic, Mirjana Petrovic, Tatjana Cosic, Mariana Stanisic, Branka Vinterhalter
Summary: In this study, an efficient tissue culture system for improving the rocket cultivar Domaca rukola was established. Optimal shoot multiplication and organogenesis protocols were determined through experiments with different hormone concentrations and combinations. In addition, in vitro-produced rocket shoots were found to be a potential source of the antioxidant flavonoid kaempferol.
Article
Plant Sciences
Sofija Stupar, Vaclav Motyka, Petre I. Dobrev, Tatjana Cosic, Nina Devrnja, Ljiljana Tubic, Jelena Savic
Summary: The study investigated the comprehensive phytohormone profile of potato plants exposed to French marigold essential oil (EO) for different time periods. The results showed altered phytohormone responses of EO-exposed potato plants, with stress-related phytohormones being the most affected. The expression levels of genes involved in biosynthesis and catabolism of stress-related phytohormones indicated that the jasmonic acid biosynthetic pathway was the most affected.
JOURNAL OF PLANT GROWTH REGULATION
(2023)
Article
Plant Sciences
Vladimir Skalicky, Ioanna Antoniadi, Ales Pencik, Ivo Chamrad, Rene Lenobel, Martin F. Kubes, Marek Zatloukal, Asta Zukauskaite, Miroslav Strnad, Karin Ljung, Ondrej Novak
Summary: Auxin and cytokinin show subcellular distribution in Arabidopsis cells, forming hormone gradients. This finding provides important insights into the mechanisms of plant growth and development.
Article
Agronomy
Dusica Calic, Jasna L. Ristic-Djurovic, Sasa Cirkovic, Jelena Milojevic, Maja Belic, Mariana Stanisic, Snezana Zdravkovic-Korac
Summary: This study demonstrates that exposing flax seeds to static magnetic fields can improve their germination and quality. Specifically, a 350 mT magnetic field treatment for 100 and 120 minutes achieved the best germination results and showed significant improvements in several biological indicators.
Article
Chemistry, Analytical
Ivan Petr, Ales Pencik, Jakub Styskala, Lenka Tranova, Petra Amakorova, Miroslav Strnad, Ondrej Novak
Summary: The study introduces a novel approach using ultra-high performance supercritical fluid chromatography coupled with tandem mass spectrometry (UHPSFC-MS/MS) for the quantification of plant hormones. Compared to traditional UHPLC-MS/MS methods, this new approach offers a shorter total run time and lower matrix effect.
ANALYTICA CHIMICA ACTA
(2024)
