Article
Chemistry, Multidisciplinary
Yongcong Fang, Yihan Guo, Bingyan Wu, Zibo Liu, Min Ye, Yuanyuan Xu, Mengke Ji, Li Chen, Bingchuan Lu, Kaiji Nie, Zixuan Wang, Jianbin Luo, Ting Zhang, Wei Sun, Zhuo Xiong
Summary: A reversible ink template-based bioprinting strategy (SPIRIT) is developed to replicate the external geometry and internal structure of complex organs. This strategy utilizes a microgel-based bioink with shear-thinning and self-healing behavior to support embedded 3D printing, resulting in the generation of cardiac tissues and organoids. This technique allows for the rapid replication of complex organ geometry and internal structures, accelerating the biofabrication and therapeutic applications of tissue and organ constructs.
ADVANCED MATERIALS
(2023)
Article
Engineering, Biomedical
Hyeongjin Lee, Carlos Kengla, Han Su Kim, Ickhee Kim, Jae-Gu Cho, Eric Renteria, Kyungsup Shin, Anthony Atala, James J. Yoo, Sang Jin Lee
Summary: Medical imaging and 3D bioprinting can be used to create patient-specific bone scaffolds with complex shapes and controlled inner architectures. This study investigates the effectiveness of a biomimetic approach to scaffold design and demonstrates that the biomimetic scaffold with a dense external layer improves bone regeneration compared to the control scaffold. Histological examination confirms the superior bone regeneration in the biomimetic scaffolds and suggests that patient-specific anatomy can be accurately translated into a 3D bioprinting strategy, leading to clinically relevant constructs.
ADVANCED HEALTHCARE MATERIALS
(2023)
Review
Pharmacology & Pharmacy
Sandra Ruiz-Alonso, Ilia Villate-Beitia, Idoia Gallego, Markel Lafuente-Merchan, Gustavo Puras, Laura Saenz-del-Burgo, Jose Luis Pedraz
Summary: Three-dimensional (3D) printing technology shows great promise for various biomedical applications, including ophthalmology, allowing for custom-fabrication of specific eye tissues in precise anatomical 3D geometries. Despite challenges, recent progress has been made in implementing 3D bioprinting technology in ophthalmology, with a focus on manufacturing tissues such as cornea, retina, and conjunctiva. Additional considerations include the use of 3D bioprinting for ocular drug delivery and ethical and regulatory aspects.
Review
Biotechnology & Applied Microbiology
Haonan Wang, Huaqing Yu, Xia Zhou, Jilong Zhang, Hongrui Zhou, Haitong Hao, Lina Ding, Huiying Li, Yanru Gu, Junchi Ma, Jianfeng Qiu, Depeng Ma
Summary: This article reviews the significance and recent progress of ECM-based biomaterials in 3D bioprinting, as well as their applications in tissue regeneration and cancer research. There is great potential for future development.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Medicine, Research & Experimental
Huihui Zhang, Yilin Wang, Zijun Zheng, Xuerong Wei, Lianglong Chen, Yaobin Wu, Wenhua Huang, Lei Yang
Summary: 3D bioprinting technology can replicate native tissue and organ microenvironments by precisely placing cells in bioinks. However, finding the ideal bioink is a challenge. Organ-derived decellularized ECM (dECM) bioink has optimal biomimetic properties but is non-printable due to poor mechanical properties. Recent studies have focused on improving the printability of dECM bioinks. This review highlights the methods used to produce these bioinks, strategies to improve printability, and advancements in tissue regeneration. Challenges and potential applications are also discussed.
Review
Engineering, Biomedical
Pu Yang, Yikun Ju, Yue Hu, Xiaoyan Xie, Bairong Fang, Lanjie Lei
Summary: This paper reviews the recent research advances in the application of 3D bioprinting in plastic surgery. It introduces the printing process and basic principles of 3D bioprinting technology, describes the currently available bioprinting materials, and analyzes the rationale for special dynamic 3D bioprinting. The focus is on the viable clinical applications and effects of 3D bioprinting in plastic surgery.
BIOMATERIALS RESEARCH
(2023)
Review
Biochemistry & Molecular Biology
Moon Sung Kang, Jinju Jang, Hyo Jung Jo, Won-Hyeon Kim, Bongju Kim, Heoung-Jae Chun, Dohyung Lim, Dong-Wook Han
Summary: This review focuses on the advances and innovations of 3D bioprinted skin for skin regeneration. By improving materials, structures, and functions, the clinical potential of 3D bioprinted skin has been enhanced. With state-of-the-art printing technology and novel biomaterials, highly developed skin comprising stratified epidermis, dermis, and hypodermis with blood vessels, nerves, muscles, and skin appendages can be fabricated. We hope that this review will guide future research and clinical applications.
Article
Biophysics
Ji Min Seok, Jae Eun Jeong, Sang Jin Lee, Seung Hyun Im, Jun Hee Lee, Wan Doo Kim, Kangwon Lee, Su A. Park
Summary: This study introduced a strategy utilizing HA nanoparticles and CSP process to enhance the mechanical and biological properties of SA material, successfully fabricating scaffolds with improved mechanical and biological properties for potential applications in bone-tissue regeneration and engineering.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Materials Science, Multidisciplinary
Yi Zhang, Piyush Kumar, Songwei Lv, Di Xiong, Hongbin Zhao, Zhiqiang Cai, Xiubo Zhao
Summary: 3D bioprinting technology combines computing science, biology, and material engineering, and has great potential in fabricating vascularized constructs for tissue engineering. This review summarizes recent advances in 3D bioprinting of vascularized tissues, including the selection of biomaterials and various 3D printing techniques and applications.
MATERIALS & DESIGN
(2021)
Review
Chemistry, Medicinal
Ying Chen, Ying Wang, Sheng-Chang Luo, Xiang Zheng, Ranjith Kumar Kankala, Shi-Bin Wang, Ai-Zheng Chen
Summary: Body articulation units, also known as body joints, are important for body flexibility in the musculoskeletal system. However, they are prone to various pathological conditions such as arthritis. Existing treatment modalities based on anti-inflammatory and analgesic drugs have limitations in terms of cost and recovery. Engineering strategies, such as fabricating body articulation unit models for tissue regeneration and drug development, have emerged as promising solutions. Challenges and difficulties still exist in the clinical application of these models.
DRUG DESIGN DEVELOPMENT AND THERAPY
(2022)
Review
Pharmacology & Pharmacy
Annika C. Dell, Grayson Wagner, Jason Own, John P. Geibel
Summary: 3D bioprinting plays a crucial role in tissue engineering in medicine by providing precise and highly customizable methods for creating biological tissues. The selection of appropriate cell ink formulations is vital for process optimization and customization to suit target tissues. Hydrogel-based cell ink formulations show promise for future development in bioprinting technology.
Review
Materials Science, Multidisciplinary
Amir A. Elhadad, Ana Rosa-Sainz, Raquel Canete, Estela Peralta, Belen Begines, Mario Balbuena, Ana Alcudia, Y. Torres
Summary: This article explores recent advancements and emerging trends in 3D printing from a novel multidisciplinary perspective. It provides an overview of various 3D printing techniques and their applications, with a focus on materials such as composites, hybrids, and smart materials. The article also discusses the latest developments in 4D bioprinting technology and outlines present challenges and future regulations.
MATERIALS SCIENCE & ENGINEERING R-REPORTS
(2023)
Article
Cell & Tissue Engineering
Leila Roshangar, Jafar Soleimani Rad, Raziyeh Kheirjou, Ahad Ferdowsi Khosroshahi
Summary: The study investigated the use of 3D bioprinter derived-gel scaffolds for accelerating wound healing, and found that ADSCs have a positive impact on epithelization speed. 3D bioprinter derived-gel scaffolds show potential for application in skin regenerative medicine.
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2021)
Article
Materials Science, Multidisciplinary
Neeha Dev Arun, Humphrey Yang, Lining Yao, Adam W. W. Feinberg
Summary: Thermally cured thermoset polymers, such as epoxies, are commonly used in industry due to their excellent thermal, chemical, and electrical resistance, as well as their mechanical properties. However, 3D printing these materials without rheological modification can be challenging as they tend to flow and lose shape during extrusion. A solution to this is 3D printing inside a support bath, which allows the liquid polymer to be held in place until fully cured. In this study, Freeform Reversible Embedding (FRE) is used to 3D print off-the-shelf thermoset epoxy into lattice structures using nonplanar extrusion, demonstrating the advantages of this approach.
ADVANCED MATERIALS TECHNOLOGIES
(2023)
Review
Polymer Science
Huaying Liu, Yuxuan Gong, Kaihui Zhang, Shen Ke, Yue Wang, Jing Wang, Haibin Wang
Summary: As an emerging 3D printing technology, 3D bioprinting is promising in tissue engineering and regenerative medicine. Decellularized extracellular matrices (dECM) are used to create unique tissue-specific bioink, and combining dECMs with 3D bioprinting can construct biomimetic hydrogels for bioinks. dECM has been proven as one of the fastest growing bioactive printing materials in cell-based 3D bioprinting.
Review
Engineering, Multidisciplinary
Abdellah Aazmi, Hongzhao Zhou, Yuting Li, Mengfei Yu, Xiaobin Xu, Yutong Wu, Liang Ma, Bin Zhang, Huayong Yang
Summary: Organ-on-a-chip technology is a promising method for cell culture that can replace animal models in preclinical testing. This review discusses the synergy between the vasculature and organ-on-a-chip technology, and the steps and applications of vascularized organ-on-a-chip fabrication.
Article
Automation & Control Systems
Hongzhao Zhou, Andrew R. Plummer, David Cleaver
Summary: This article introduces an active tensegrity structure as the basis for a morphing wing. An experimental device has been designed and built to demonstrate the accuracy of shape control and aerodynamic performance of the prototype morphing wing through wind tunnel tests.
IEEE-ASME TRANSACTIONS ON MECHATRONICS
(2022)
Article
Biotechnology & Applied Microbiology
Linh T. B. Nguyen, Timothee Baudequin, Zhanfeng Cui, Hua Ye
Summary: In this study, polycaprolactone (PCL) macrobeads conjugated with poly(N-isopropylacrylamide) (PNIPAAm) were prepared as cell carriers with noninvasive cell detachment properties (thermo-response). Homemade macrobeads were shown to have advantages in controlling morphological, biological, and fluidization properties compared to commercial beads. The PNIPAAm-functionalized macrobeads allowed mesenchymal stem cells (MSCs) to spread and grow, and successfully detached the MSCs at reduced temperature. This research also demonstrated the potential for large-scale production of thermo-responsive macrocarriers.
BIOTECHNOLOGY AND BIOENGINEERING
(2022)
Review
Cell & Tissue Engineering
Huidong Jia, Jia He, Lan Zhao, Chia-Chen Hsu, Xiaofeng Zhao, Yuzheng Du, Lin Han, Zhanfeng Cui, Xuemin Shi, Hua Ye
Summary: This article discusses the potential benefits of combining stem cell therapy and acupuncture in treating stroke patients. It aims to uncover the possible mechanisms and demonstrate the potential improvement in clinical outcomes.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Biochemical Research Methods
Boon Lim, Yutong Yin, Hua Ye, Zhanfeng Cui, Antonis Papachristodoulou, Wei E. Huang
Summary: Advances in synthetic biology have enabled the reprogramming of bacteria to specifically target tumors and release anticancer drugs in a controlled manner. SimCells, chromosome-free bacteria controlled by gene circuits, have been engineered to display antibodies on their surface and effectively bind and induce death of cancer cells expressing specific biomarkers. These findings highlight the potential of SimCells for targeted cancer therapy and the application of synthetic biology in medicine.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Si-Yuen Lee, Julian George, David Nagel, Hua Ye, Leonard Seymour
Summary: This study proposed a 3D platform based on RGD-alginate for encapsulating optogenetically modified human neuronal cells, demonstrating their survival and ability to express functional neurons.
Article
Biochemistry & Molecular Biology
Niko E. Putra, Marius A. Leeflang, Verena Ducret, Viorica Patrulea, Lidy E. Fratila-Apachitei, Karl Perron, Hua Ye, Jie Zhou, Iulian Apachitei, Amir A. Zadpoor
Summary: Surface biofunctionalization of porous titanium with Ag and Fe NPs is an effective strategy to prevent implant-associated infections and promote bone regeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Risto Martin, Rui Lei, Yida Zeng, Jiachen Zhu, Hong Chang, Hua Ye, Zhanfeng Cui
Summary: Stem cell and cell therapies are increasingly common in clinical practice. However, their high cost poses a barrier to widespread application. Membrane technologies, such as hollow fiber membrane bioreactors, have the potential to significantly reduce the cost of autologous cell therapies by reshaping bioprocessing and manufacture of cellular products.
Article
Biotechnology & Applied Microbiology
Timothee Baudequin, Hazel Wee, Zhanfeng Cui, Hua Ye
Summary: Micro-carriers, such as Ca-alginate and Fe-alginate, have been studied as substrates for mesenchymal stem cell (MSCs) proliferation. While Ca-alginate requires functionalization, Fe-alginate beads showed great potential as ready-to-use carriers for MSCs without the need for additional modifications.
BIOENGINEERING-BASEL
(2023)
Article
Polymer Science
Guoying Zhou, Jiayan Zhu, Catriona Inverarity, Yifeng Fang, Zhao Zhang, Hua Ye, Zhanfeng Cui, Linh Nguyen, Haitong Wan, Julian F. Dye
Summary: A fibrin/PVA scaffold with interconnected porous structures and preserved fibrous architecture was fabricated using an emulsion templating method. The scaffold showed excellent biocompatibility and efficacy for dermal reconstruction, promoting wound healing and tissue regeneration.
Article
Engineering, Manufacturing
Ziqi Gao, Jun Yin, Peng Liu, Qi Li, Runan Zhang, Huayong Yang, Hongzhao Zhou
Summary: To mimic natural tissue, multi-material bioprinting is commonly used to construct tissue models in vitro. The current sequential multi-material embedded printing method has a trade-off between structural integrity and printing precision. We propose a simultaneous multi-material embedded printing method that can print firmly attached and high-precision multilayer structures. This method minimizes uncontrolled squeezing and contamination, and offers better structural integrity than conventional sequential printing methods, making it a powerful tool for personalized medicine.
INTERNATIONAL JOURNAL OF EXTREME MANUFACTURING
(2023)
Article
Physics, Multidisciplinary
Casey Adam, Celine Kayal, Ari Ercole, Sonia Contera, Hua Ye, Antoine Jerusalem
Summary: This study demonstrates that general anaesthetics affect the viscoelasticity and functional activity of cells simultaneously, and that the alterations in firing and viscoelasticity are correlated.
COMMUNICATIONS PHYSICS
(2023)
Article
Biotechnology & Applied Microbiology
Carla V. Fuenteslopez, Mark S. Thompson, Hua Ye
Summary: Traumatic injuries are a significant global health issue, but there is limited research on microvascular traumatic injuries. This study aims to develop an in vitro model for studying traumatic injuries at the microvascular level. Different hydrogels were created using various polymers, solvents, and concentrations, and evaluated for their effects on cell behavior and hydrogel properties. Fibrin hydrogels at 3% and 5% w/v in serum-free media were found to be the most suitable for further experimentation, enabling the formation of interconnected capillary-like networks.
BIOENGINEERING-BASEL
(2023)