Review
Cell Biology
Fredrik Noborn, Mahnaz Nikpour, Andrea Persson, Jonas Nilsson, Goran Larson
Summary: Chondroitin sulfate proteoglycans are a type of proteoglycan found on cell surfaces and in connective tissues, interacting with proteins involved in various pathophysiological processes. Recent advances in glycoproteomic methods have allowed for the identification of numerous novel chondroitin sulfate core proteins and attachment sites in humans and animal models, revealing greater structural complexity of CSPGs than previously perceived. These findings may challenge traditional notions of defining proteoglycans based on function or structural evidence.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemical Research Methods
Margaret Downs, Jillian Curran, Joseph Zaia, Manveen K. Sethi
Summary: Proteoglycans are a diverse family of proteins that play various roles at the cell surface and extracellular matrix. This study presents a method combining serial proteolysis, higher-energy collisional dissociation (HCD), and electron transfer/higher-energy collisional dissociation (EThcD) to optimize protein sequence coverage and glycopeptide identification from proteoglycans. The use of EThcD allows the confident assignment of glycan compositions on multiply glycosylated peptides. The development of analytical tools for glycoproteomic similarity comparisons in biological samples is of great interest.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Review
Spectroscopy
Margaret Downs, Joseph Zaia, Manveen K. Sethi
Summary: The brain extracellular matrix (ECM), which is highly glycosylated, plays important roles in cell communication and neural function. Neuroprotection and neural plasticity rely on the formation of structures such as perineuronal nets (PNNs), which are influenced by glycans. Disorders like Alzheimer's disease, Parkinson's disease, and Schizophrenia are associated with dysregulation of ECM molecules and changes in glycomic profiles. Understanding these changes in brain diseases may lead to new therapeutic approaches.
MASS SPECTROMETRY REVIEWS
(2022)
Article
Chemistry, Physical
Haiyi Chen, Yu Kang, Mojie Duan, Tingjun Hou
Summary: The study revealed that the presence of heparan sulfate is crucial for the binding between the spike protein of SARS-CoV-2 and host ACE2, while the stability of the T470-F490 loop and hydrophobic interactions play key roles in the binding process.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2021)
Article
Biochemistry & Molecular Biology
Linh Nguyen, Kelli A. McCord, Duong T. Bui, Kim M. Bouwman, Elena N. Kitova, Mohamed Elaish, Dhanraj Kumawat, Gour C. Daskhan, Ilhan Tomris, Ling Han, Pradeep Chopra, Tzu-Jing Yang, Steven D. Willows, Andrew L. Mason, Lara K. Mahal, Todd L. Lowary, Lori J. West, Shang-Te Danny Hsu, Tom Hobman, Stephen M. Tompkins, Geert-Jan Boons, Robert P. de Vries, Matthew S. Macauley, John S. Klassen
Summary: Evidence suggests that host glycans, specifically glycolipids containing sialic acid, play a role in facilitating the entry of SARS-CoV-2 virus into host cells by binding to the receptor-binding domain (RBD) of the spike protein. Depletion of cell surface sialic acid levels through various methods decreases RBD binding and infection of the virus, indicating the importance of sialylated glycans in viral entry.
NATURE CHEMICAL BIOLOGY
(2022)
Article
Chemistry, Physical
Saber Shakibi, Patrick R. R. Onck, Erik van der Giessen
Summary: Glycosaminoglycans (GAGs) are polysaccharides that play crucial roles in biological processes. They are important structural components of cartilage and the extracellular matrix of the brain. Coarse-grained modeling is essential due to the large size of GAGs.
JOURNAL OF CHEMICAL THEORY AND COMPUTATION
(2023)
Article
Biochemistry & Molecular Biology
Kento Shionoya, Takehiro Suzuki, Mako Takada, Kazuki Sato, Shoichi Onishi, Naoshi Dohmae, Koichiro Nishino, Takeshi Wada, Robert J. Linhardt, Toshihiko Toida, Kyohei Higashi
Summary: This study conducted a comprehensive analysis of cartilage in the heads of 10 bony fishes, including sturgeon and salmon. The results revealed that the structure of chondroitin sulfate (CS) in sturgeons and salmon differed from other bony fishes, and aggrecan was identified as the major core protein in all bony fish head cartilage.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biology
Lynn Kruger, Karina Biskup, Vasileios Karampelas, Antje Ludwig, Antje-Susanne Kasper, Wolfram C. Poller, Veronique Blanchard
Summary: In this study, an optimized strategy was presented to analyze glycosaminoglycan disaccharides via MALDI-TOF mass spectrometry, successfully identifying sulfation positions and patterns through fragmentation experiments.
Article
Oncology
Gabor Toth, Simon Sugar, Domonkos Pal, Kata Dorina Fugedi, Laszlo Drahos, Gitta Schlosser, Csilla Olah, Henning Reis, Ilona Kovalszky, Tibor Szarvas, Lilla Turiak
Summary: This study investigated the differences in glycosaminoglycan disaccharides between patients with prostate cancer and benign prostate hyperplasia. The study found that the sulfation of chondroitin sulfate chains and the levels of doubly and triply sulfated heparan sulfate disaccharides were significantly different between the two groups. These disaccharides were found to be independent biomarkers for survival.
Article
Biochemical Research Methods
Gustavo J. Cavallero, Yan Wang, Charles Nwosu, Sheng Gu, Muthuraman Meiyappan, Joseph Zaia
Summary: Recombinant protein engineering design affects therapeutic properties including protein efficacy, safety, and immunogenicity. Glycosylation modulates glycoprotein therapeutic pharmacokinetics, pharmacodynamics, and effector functions. NanoHILIC-MS is advantageous in elucidating glycan features on glycotherapeutics that fail to be detected using traditional methods.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Applied
Shoichi Onishi, Kento Shionoya, Kazuki Sato, Ayumu Mubuchi, Shiori Maruyama, Tadaaki Nakajima, Masahiro Komeno, Shinji Miyata, Kazumi Yoshizawa, Takeshi Wada, Robert J. Linhardt, Toshihiko Toida, Kyohei Higashi
Summary: The study investigates the glycosaminoglycans (GAGs) derived from marine organisms and finds that marine invertebrates, especially Bivalvia, are rich sources of highly sulfated or branched GAGs. A novel fucosylated heparan sulfate (Fuc-HS) is identified from the midgut gland of the Japanese scallop, Patinopecten yessoensis. Scallop HS exhibits resistance to GAG-degrading enzymes and shows anti-coagulant and neurite outgrowth-promoting activities. These findings suggest that the midgut gland of scallops is a valuable source of biologically active Fuc-HS.
CARBOHYDRATE POLYMERS
(2023)
Article
Biochemistry & Molecular Biology
Maria Ennemoser, Alexandra Pum, Andreas Kungl
Summary: This review investigates the connection between modifications of GAG sulfation patterns and a wide range of pathological conditions, mainly focusing on a range of chronic diseases of the central nervous system (CNS) as well as the respiratory tract.
CARBOHYDRATE RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Eduardo Stancanelli, Wei Liu, Rylee Wander, Jine Li, Zhangjie Wang, Katelyn Arnold, Guowei Su, Adam Kanack, Truong Quang Pham, Vijayakanth Pagadala, Anand Padmanabhan, Yongmei Xu, Jian Liu
Summary: A library of structurally homogeneous HS and CS chimeric dodecasaccharides (12-mers) has been synthesized using natural biosynthetic enzymes. These chimeras exhibit anticoagulant activity and can be reversibly neutralized by protamine, offering a new class of glycan molecules for biological research.
ACS CHEMICAL BIOLOGY
(2022)
Article
Cell Biology
Chia-Chen Liu, Melissa E. Murray, Xia Li, Na Zhao, Na Wang, Michael G. Heckman, Francis Shue, Yuka Martens, Yonghe Li, Ana-Caroline Raulin, Cassandra L. Rosenberg, Sydney Doss, Jing Zhao, Melissa C. Wren, Lin Jia, Yingxue Ren, Tadafumi C. Ikezu, Wenyan Lu, Yuan Fu, Thomas Caulfield, Zachary A. Trottier, Joshua Knight, Yixing Chen, Cynthia Linares, Xue Wang, Aishe Kurti, Yan W. Asmann, Zbigniew K. Wszolek, Glenn E. Smith, Prashanthi Vemuri, Kejal Kantarci, David S. Knopman, Val J. Lowe, Clifford R. Jr Jr Jack, Joseph E. Parisi, Tanis J. Ferman, Bradley F. Boeve, Neill R. Graff-Radford, Ronald C. Petersen, Steven G. Younkin, John D. Fryer, Hu Wang, Xianlin Han, Carl Frieden, Dennis W. Dickson, Owen A. Ross, Guojun Bu
Summary: The APOE3-Jacksonville variant has been shown to reduce APOE aggregation and enhance its lipidation in human brains, leading to increased levels of key lipids critical for synaptic functions. Mice expressing APOE3-Jac exhibit reduced amyloid pathology, plaque-associated immune responses, and neuritic dystrophy, indicating potential therapeutic targets for treating individuals with AD and DLB.
SCIENCE TRANSLATIONAL MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Ayumu Mubuchi, Shoo Katsumoto, Makoto Tsuboi, Hideaki Ishikawa, Yoshihiro Nomura, Kyohei Higashi, Shinji Miyata
Summary: Chondroitin sulfate and heparan sulfate are sulfated glycosaminoglycan chains with different levels of sulfation and disaccharide compositions. The sulfated glycosaminoglycans from Perna canaliculus show potential as biomaterials for studying the structure-function relationships.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)