Optimising the zoospore release, germination, development of gametophytes and formation of sporophytes of Ecklonia radiata

The kelp Ecklonia radiata has become a target for controlled cultivation. However, to date there are no standardised protocols for the hatchery stage of this species that result in high rates of germination, gametophyte development and transition to sporophytes. Therefore, the objective of this study was to quantify the effect of photoperiod, light intensity, temperature, nutrient media and use of GeO2 on the key hatchery processes of germination, gametophyte development and transition to sporophytes in controlled laboratory experiments. Germination of E. radiata was high (>= 85%) throughout the study, regardless of treatments. Temperature had a major effect on the length of gametophytes, which increased with increasing temperature. The formation of sporophytes was favoured when individuals were maintained under 17 degrees C continuously, while reduced by approximately 30% when using F/2 compared to PES nutrient media. Overall, the recommended conditions for the hatchery stage of E. radiata are to maintain cultures under a 12 h L:12 h D photoperiod at 17 degrees C as this resulted in higher germination rates, good gametophyte development and higher transition to sporophytes compared to other treatments. Moreover, the use of GeO2 has to be limited to no more than 2 days as extended use has detrimental effects on the development of sporophytes. Finally, storage of sorus-bearing fronds of sporophytes up to 4 days after the collection from the field generally increased the number of released zoospores and is a simple mechanism to increase the fertility of brood stock.

Automated summary

This study aims to quantify the impact of photoperiod, light intensity, temperature, nutrient media, and GeO2 on the key hatchery processes of germination, gametophyte development, and transition to sporophytes in the controlled cultivation of Ecklonia radiata. The results show that maintaining a 12 hour light:12 hour dark photoperiod at 17 degrees Celsius leads to higher germination rates, good gametophyte development, and higher transition to sporophytes. Additionally, limiting the use of GeO2 and extending the storage time of sorus-bearing fronds of sporophytes can increase the fertility of the brood stock.