Article
Chemistry, Analytical
Shao-Yu Liang, Shih-Chih Wan, Yen-Peng Ho, Yu-Tze Horng, Po-Chi Soo, Wen-Ping Peng
Summary: A new approach using LIRFP CD QIT-MS to rapidly quantify PHB content is presented in this study. Compared to GC, LIRFP CD QIT-MS offers advantages of shorter analysis time and no use of hazardous chemicals. This method has the potential for routine monitoring of PHB production.
ANALYTICAL CHEMISTRY
(2022)
Review
Agricultural Engineering
Jia-Shun Cao, Run-Ze Xu, Jing-Yang Luo, Qian Feng, Fang Fang
Summary: Polyhydroxyalkanoates (PHA) are promising bioplastics with excellent physicochemical properties and biodegradability, but suffer from high manufacturing costs. To reduce costs, researchers have conducted experiments with mixed microbial cultures and low-cost substrates, requiring rapid and reliable PHA quantification methods. This review provides a comprehensive understanding of PHA fluorescence quantification (PHA-FQ) methods, including performance evaluation, spectral properties, staining procedures, and current developments, aiming to aid researchers in improving PHA-FQ methods.
BIORESOURCE TECHNOLOGY
(2022)
Article
Chemistry, Physical
Yuanyuan Wei, Syed Muhammad Tariq Abbasi, Nawaz Mehmood, Luoquan Li, Fuyang Qu, Guangyao Cheng, Dehua Hu, Yi-Ping Ho, Wu Yuan, Ho-Pui Ho
Summary: The study presents a deep learning-aided algorithm called Deep-qGFP for real-time absolute quantification. It enables automated detection and classification of fluorescent-labeled microreactors, accurately measuring their sizes and occupancy status. Deep-qGFP achieves high accuracy and remarkable speed, making it applicable to various GFP-labeling scenarios. It can be seamlessly integrated into common fluorescence microscopes and different microreactor formats, optimizing laboratory practices and potentially transforming GFP-labeled microreactor analysis.
Article
Biotechnology & Applied Microbiology
Bernard O. Boateng, Saioa Elcoroaristizabal, Alan G. Ryder
Summary: This study developed a new method for protein quantification in bioreactor broth using polarized total synchronous fluorescence spectroscopy, which showed better analytical performance compared to traditional methods. By optimizing the process, faster online measurements and more accurate results were achieved.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Biotechnology & Applied Microbiology
Alexander Kettner, Matthias Noll, Carola Griehl
Summary: Fluorescence spectroscopy combined with side scatter was used to monitor the biomass and poly(3-hydroxybutyrate) (PHB) formation in biotechnological applications. Different dyes were compared for PHB quantification, and the results showed high accuracy and transferability. This approach provides a fast and simultaneous detection method for monitoring biomass and PHB content in fermentation processes.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Spectroscopy
Paramita Saha, Murali Monohar Pandey
Summary: A new fluorescence-based method for estimating rotigotine in bulk and nanoformulations has been developed and validated. The method demonstrates good linearity and accuracy, and is specific and robust for use in pharmaceutical dosage forms.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Chemistry, Applied
Ana Gonzalez de Peredo, Mercedes Vazquez-Espinosa, Zulema Pineiro, Estrella Espada-Bellido, Marta Ferreiro-Gonzalez, Gerardo F. Barbero, Miguel Palma
Summary: A UHPLC method was developed in this study to selectively and accurately determine the main phenolic compounds in grapes, demonstrating excellent separation and detection capabilities. The method was validated for real matrix sample analysis of several grape varieties.
Article
Chemistry, Analytical
Yafeng Liu, Daizheng Liu, Zhenming Xie
Summary: A rapid, sensitive, and inexpensive method for quantification of arbutin in cosmetic products was established using sodium hypochlorite oxidation. The method showed linear relation between fluorescence intensity and arbutin concentration, with good recovery rate, and was successfully applied in the analysis of marketed cosmetis containing arbutin.
ANALYTICAL LETTERS
(2022)
Article
Food Science & Technology
Zhiyun Zhang, Helen Lin, Chuanxin Ma, Lingzi Zhang, Julia Zelevinsky, Ming Xia, Yuecheng Xu, Jason C. White, Julian Tyson, Lili He
Summary: The integrated Gutzeit method with XRF spectroscopy provides a sensitive, reliable, fast, and cost-effective way to measure inorganic arsenic in beverages. The method was validated with commercial beverages and compared with other arsenic testing methods.
Article
Biochemical Research Methods
Kari Kopra, Nazia Hassan, Emmiliisa Vuorinen, Salla Valtonen, Randa Mahran, Huda Habib, Pinja Jalkanen, Petri Susi, Vesa Hytonen, Minna Hankaniemi, Seppo Yla-Herttuala, Laura Kakkola, Markus Peurla, Harri Harma
Summary: Viruses play a major role in modern society, but current methods for counting viral particles are cumbersome and expensive. To address this issue, researchers have developed a simple and cost-effective time-resolved luminescence-based method that allows for rapid measurement of virus particle count.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Food Science & Technology
Hongfang Li, Jie Chen, Baowei Huang, Lingwei Kong, Feifei Sun, Lin Li, Chuanyi Peng, Huimei Cai, Ruyan Hou
Summary: A fluorescence sensor for the direct quantification of Rongalite was developed, which offers advantages of rapid detection, high selectivity and accuracy. It has promising application potential for the rapid detection of Rongalite in foodstuffs.
Article
Engineering, Biomedical
Carolina Paganini, Britta Hettich, Marie R. G. Kopp, Adam Eordogh, Umberto Capasso Palmiero, Giorgia Adamo, Nicolas Touzet, Mauro Manno, Antonella Bongiovanni, Pablo Rivera-Fuentes, Jean-Christophe Leroux, Paolo Arosio
Summary: This microfluidic technique allows for rapid detection and quantification of components associated with EV subtypes and impurities, measuring EV purity in minutes with minimal sample handling and less than 5 μL of sample. It accelerates screening of EV samples, aids in evaluating sample reproducibility, and serves as an important complementary tool to current biophysical methods for EV characterization.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Spectroscopy
Zongcheng Wang, Weikang Su, Huihuang Zheng, Shun Yang, Tingting Yang, Ting Han, Wubliker Dessie, Xingrui He, Yuren Jiang, Yuanqiang Hao
Summary: Fluorescence probe detection of selenocysteine (Sec) content in cells is important for identifying human related diseases. Two turn-on fluorescent probes, A4 and B4, were designed and synthesized for fast and sensitive detection of Sec. Both A4 and B4 show rapid turn-on response, high selectivity, and sensitivity towards Sec, making them applicable for fluorescence imaging of Sec in living cells. A4 has a larger Stokes shift, wider pH range, and lower detection limit compared to B4, due to its excited state intramolecular proton transfer (ESIPT) effect. These two probes can serve as effective tools for detecting Sec in biological systems.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Chemistry, Inorganic & Nuclear
Hongmei Zhang, Xiangjun Guo, Ke Jian, Liming Fu, Xihui Zhao
Summary: Three types of long-wavelength emissive fluorescent carbon dots, yellow, orange, and red, were rapidly prepared using a microwave-assisted method and successfully used for information encryption. The synthesis of long-wavelength emission fluorescent carbon dots is not common, and it is even more difficult to quickly synthesize within 10 min. The synthesized carbon dots with different B, N doping contents showed differences in their surface structures and luminescence properties, making them suitable for information encryption.
INORGANIC CHEMISTRY
(2023)
Article
Environmental Sciences
Matteo Giardino, Valentina Balestra, Davide Janner, Rossana Bellopede
Summary: Microplastics are small solid polymers with dimensions less than 5 mm that contaminate the environment. Visual count is commonly used for their detection and quantification, but it is time-consuming and prone to errors. To address this issue, a semi-automatic image processing method was developed for quantifying and measuring microplastics on filter membrane substrates, providing accurate and fast results.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Review
Biotechnology & Applied Microbiology
Ruchira Mitra, Tong Xu, Guo-Qiang Chen, Hua Xiang, Jing Han
Summary: Polyhydroxyalkanoates (PHA) synthesis is tightly regulated by multiple regulatory networks impacting PHA content, granule size, and distribution in cells. Diverse regulatory circuits operate in important PHA-producing microorganisms, with the need for further research in some organisms.
MICROBIAL BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Zihua Li, Yudian Zhang, Meng Han, Haiteng Deng, Fuqing Wu, Gang Liu, Guo-Qiang Chen
Summary: Beta-hydroxybutyrate (3HB) is a ketone body in mammals that not only provides energy for the body, but also participates in cell signal transduction. This study focuses on the immunomodulatory mechanisms of 3HB, showing that it can post-translationally modify antibodies with Kbhb to improve their stability.
Article
Agricultural Engineering
Supratim Ghosh, Jim Coons, Chris Yeager, Peter Halley, Alexander Chemodanov, Bogdan Belgorodsky, Michael Gozin, Guo-Qiang Chen, Alexander Golberg
Summary: The study successfully demonstrated the outdoor cultivation of Haloferax mediterranei for PHA production from green macroalgae Ulva sp. using pneumatically agitated bioreactors and ultrasonic separation. Results showed scalable processes with enhanced settling of archaeal cells, providing new technologies for halophilic biorefinery. The research highlighted the importance of optimizing cultivation conditions and harvest methods for efficient production of PHA from renewable sources.
BIORESOURCE TECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Ziyu Wang, Yifei Zheng, Mengke Ji, Xu Zhang, Huan Wang, Yuemeng Chen, Qiong Wu, Guo-Qiang Chen
Summary: This study reveals the enhanced properties of Halomonas bluephagenesis by deleting two secondary acyltransferases in the bacterial outer membrane. These deletions lead to improved production of poly(3-hydroxybutyrate) and reduced endotoxin production.
MICROBIAL BIOTECHNOLOGY
(2022)
Article
Agricultural Engineering
Cuihuan Zhao, Taoran Zheng, Yinghao Feng, Xuan Wang, Lizhan Zhang, Qitiao Hu, Jinchun Chen, Fuqing Wu, Guo-Qiang Chen
Summary: This study successfully engineered Halomonas spp. for the production of L-lysine and cadaverine by manipulating gene expression and metabolic pathways.
BIORESOURCE TECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Matthew Russell, Andrew Currin, William Rowe, Guo-Qiang Chen, Perdita Barran, Nigel S. Scrutton
Summary: Despite its environmental benefits, biomanufacturing is not financially competitive compared to the carbon emitting chemical industry. The use of novel organisms, such as halophilic bacteria, can help reduce costs by thriving in environments unsuitable for other organisms. However, the lack of annotation for these organisms requires rapid characterization and data sharing to facilitate their adoption in biomanufacturing.
Article
Multidisciplinary Sciences
Jinfei Hou, Xu Zhang, Yuqiong Wu, Junjin Jie, Zhenxing Wang, Guo-Qiang Chen, Jiaming Sun, Lin-Ping Wu
Summary: Organohydrogels with stability and good biocompatibility were successfully fabricated using click chemistry and the combination of two-dimensional materials. These hydrogels demonstrated fatigue resistance, long-term stability in body fluids, and exhibited excellent performance in in vitro and in vivo experiments.
Article
Biology
Tong Xu, Junyu Chen, Ruchira Mitra, Lin Lin, Zhengwei Xie, Guo-Qiang Chen, Hua Xiang, Jing Han
Summary: This study successfully deleted non-essential genes in H. bluephagenesis using an optimized genome editing strategy, resulting in a genome-reduced mutant. The mutant exhibited excellent industrial properties, including rapid self-flocculation and increased DNA transformation efficiency. It maintained the ability to produce polyhydroxybutyrate (PHB) while reducing energy and carbon source requirements.
COMMUNICATIONS BIOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
Guo-Qiang Chen, Xu Zhang, Xu Liu, Weiran Huang, Zhengwei Xie, Jing Han, Tong Xu, Ruchira Mitra, Cheng Zhou, Jing Zhang, Tao Chen
Summary: This paper reviews the progress made in the last 10 years regarding Halomonas spp., a robust microbial cell factory that has been engineered to produce various biopolyesters, proteins, and small molecular compounds.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Xiao-Ran Jiang, Jie Hou, Cong-Han Wang, Guo-Qiang Chen
Summary: A highly efficient gene expression regulation system (PrrF1-2-HfqPa) was developed in Halomonas bluephagenesis, which can increase the production of 3-hydroxyvalerate fraction and the size of polyhydroxybutyrate granules. Large-scale target identification was conducted using an sRNA library targeting 30 genes to increase mevalonate production.
METABOLIC ENGINEERING
(2022)
Article
Nanoscience & Nanotechnology
Jian Li, Jiang-Nan Chen, Zi-Xin Peng, Ning-Bo Chen, Cheng-Bo Liu, Peng Zhang, Xu Zhang, Guo-Qiang Chen
Summary: In order to treat large-scale wounds or chronic ulcers, a poly(3-hydroxybutyrate-co-4-hydroxybuty-rate) (P34HB) fibrous wound dressing that integrates an antibacterial ciprofloxacin (CIP) and pro-angiogenic dimethyloxalylglycine (DMOG) was successfully prepared. The resulting dressing showed excellent biocompatibility, increased cell proliferation, migration, and capillary-like tube formation. The dressings also exhibited broad-spectrum antimicrobial action and promoted wound closure, reepithelialization, collagen formation, angiogenesis, and hair follicle regeneration. This work provides a simple and efficient approach for the design of versatile wound dressings with antibacterial and angiogenic properties.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Biotechnology & Applied Microbiology
Helen Park, Helen S. Toogood, Guo-Qiang Chen, Nigel S. Scrutton
Summary: The engineered strain Halomonas bluephagenesis TD1.0 was able to produce propane, bioplastic PHB, and biochemicals mandelate and hydroxymandelate in a single fermentation process. Multi-product separation was achieved, showcasing the versatility of biotechnology. This achievement further establishes H. bluephagenesis as a valuable platform for industrial biotechnology.
MICROBIAL BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Lizhan Zhang, Yina Lin, Xueqing Yi, Wuzhe Huang, Qitiao Hu, Zhongnan Zhang, Fuqing Wu, Jian-wen Ye, Guo-Qiang Chen
Summary: Using plasma irradiation, low-salt mutants of Halomonas bluephagenesis were obtained. Through genetic engineering, the strain H. bluephagenesis TDH4A1B5P was constructed, resulting in increased production of PHA, PHB, P34HB, ectoine, and threonine. Comparative genomic analysis identified 101 genes related to salt tolerance, and recombinant H. bluephagenesis TDH4A1B5P exhibited reduced production costs of PHA.
METABOLIC ENGINEERING
(2023)
Review
Biochemistry & Molecular Biology
Xu Yan, Xu Liu, Cuihuan Zhao, Guo-Qiang Chen
Summary: Synthetic biology aims to design or assemble existing bioparts or bio-components for useful bioproperties. Progress has been made in building biocircuits, developing genomic/metabolic engineering tools, and meeting medical and pharmaceutical demands. Synthetic biology brings new capabilities to medical and pharmaceutical researches, such as efficient production of medical agents, enhanced yields of natural products, tumor targeting, and controllable releases of therapeutic agents.
SIGNAL TRANSDUCTION AND TARGETED THERAPY
(2023)
Article
Materials Science, Biomaterials
Xu Zhang, Jian Li, Jin Chen, Zi-Xin Peng, Jiang-Nan Chen, Xinyi Liu, Fuqing Wu, Peng Zhang, Guo-Qiang Chen
Summary: The hierarchical 3D-printed P34HB scaffolds were functionalized with polydopamine-mediated BMP2, leading to enhanced bone formation in rats with bone defects. This study developed a versatile platform for bone tissue regeneration.
JOURNAL OF MATERIALS CHEMISTRY B
(2022)