Article
Environmental Sciences
Fabio Varriale, Luciana Tartaglione, Sevasti-Kiriaki Zervou, Christopher O. Miles, Hanna Mazur-Marzec, Theodoros M. Triantis, Triantafyllos Kaloudis, Anastasia Hiskia, Carmela Dell'Aversano
Summary: Cyanobacteria produce various bioactive secondary metabolites, including cyanotoxins, which are a global threat to humans and other organisms. Mass spectrometry-based methods have been developed for the determination of microcystins, the most studied class of cyanotoxins. This study compares targeted and untargeted liquid chromatography-mass spectrometry approaches for analyzing cyanobacterial biomass. It also describes the implementation of an analytical workflow for the identification of known and discovery of new cyanopeptides. The study reports the elucidation of new structural variants of microcystins and the proposal of structures for new cyanopeptides.
Review
Chemistry, Analytical
Khaja Moinuddin Shaik, Bhaskar Sarmah, Gaurav Suresh Wadekar, Pramod Kumar
Summary: This review discusses the recent detection of nitrosamine impurities in pharmaceutical products, including various detection methods, mechanisms of action, sample preparation techniques, and regulatory limits.
CRITICAL REVIEWS IN ANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Sascha K. Manier, Lea Wagmann, Armin A. Weber, Markus R. Meyer
Summary: This study aimed to investigate the detectability of main ingredients and metabolites of nutmeg seeds in human blood and urine samples using liquid chromatography coupled to mass spectrometry. Results showed that liquid chromatography techniques were successful in detecting myristicin, safrole, and other metabolites in samples, revealing sulfation as an additional Phase II metabolic pathway. These findings may aid in detecting or confirming intoxications with nutmeg seeds in the future.
DRUG TESTING AND ANALYSIS
(2021)
Article
Biochemical Research Methods
Fayaj A. Mulani, Sharvani S. Nandikol, Jagadeesh S. Kajjihundi, Niranjana Pathappa, Sharanappa Puttappa, Hirekodathakallu Thulasiram
Summary: Meliaceae plants are widely used in agriculture, folklore, and traditional medicine, and contain diverse limonoids with various bioactivities. Accurate detection of these compounds from plant tissue extracts or biological samples is challenging. In this study, a new method was developed for the identification, quantification, and characterization of limonoids in different plant tissue extracts using minimal plant material.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Medicinal
Pablo Estevez, David Castro, Jose Manuel Leao-Martins, Manoella Sibat, Angels Tudo, Robert Dickey, Jorge Diogene, Philipp Hess, Ana Gago-Martinez
Summary: This study focused on the identification of toxic compounds produced by a G. australes strain from the Mediterranean Sea, revealing the presence of a novel MTX analogue named MTX5 and the absence of CTX precursors, highlighting the complexity of identifying CTX precursors in these regions.
Article
Biochemical Research Methods
Alice Ameline, Laurie Gheddar, Jean-Sebastien Raul, Pascal Kintz
Summary: Letrozole, a reversible aromatase inhibitor used in cancer treatment, has been observed to be abused as a doping agent in high level athletes, even though it has no medical indications for men. A specific method has been developed to identify Letrozole in human hair, providing a more precise means for anti-doping agencies to detect its presence in athletes.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
Article
Chemistry, Analytical
Raegyn B. Taylor, Yelena Sapozhnikova
Summary: This study evaluated and compared the performance of two instruments for the determination of per- and polyfluoroalkyl substances (PFAS) using a newly developed sample preparation method. The results showed that the method had better recoveries, matrix interferences, and co-extractive removal compared to the FDA and FSIS methods. Validation of the method in different matrices achieved acceptable recoveries and precision for the target analytes at various levels. The study also demonstrated the potential for future analysis of emerging alternatives using high-resolution mass spectrometry.
ANALYTICA CHIMICA ACTA
(2022)
Article
Pharmacology & Pharmacy
Romain Pelletier, Thomas Gicquel, Melanie Simoes Eugenio, Pierre-Jean Ferron, Isabelle Morel, Claire Delehouze, Marie-Therese Dimanche-Boitrel, Morgane Rousselot, Brendan Le Dare
Summary: This study investigated the metabolic fate of Sibiriline using in silico prediction, in vitro and in vivo experiments. LC-HRMS/MS analysis identified 14 metabolites, mainly produced by Phase II transformations. These data provide initial reassurance regarding the toxicology of this new RIPK1 inhibitor, although further studies are required.
Article
Biochemical Research Methods
Giorgio Saluti, Matteo Ricci, Federica Castellani, Maria Novella Colagrande, Gabriella Di Bari, Michele Podaliri Vulpiani, Francesco Cerasoli, Giovanni Savini, Giampiero Scortichini, Nicola D'Alterio
Summary: Comparing the hair cortisol concentrations (HCC) of 47 horses under different managements, this study found that liquid chromatography coupled to hybrid high-resolution mass spectrometry (LC-HRMS/MS) provided more accurate results compared to the enzyme-linked immunosorbent assay (ELISA), which may overestimate the HCC. This finding is of significance in the development of accurate and reliable methods for monitoring stress in animals.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Toxicology
Selina Hemmer, Lea Wagmann, Markus R. Meyer
Summary: The study utilized untargeted metabolomics to investigate the acute effects of amphetamine on the plasma and urinary metabolome in rats. It revealed a downregulation of amino acids in plasma samples after amphetamine exposure, and identified four new potential biomarkers in urine. The research highlights the need for further studies to elucidate altered metabolic pathways associated with acute amphetamine exposure.
ARCHIVES OF TOXICOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Francesco Cerasoli, Michele Podaliri Vulpiani, Giorgio Saluti, Annamaria Conte, Matteo Ricci, Giovanni Savini, Nicola D'Alterio
Summary: This study evaluated the stress levels in horses with different management and activities by analyzing cortisol levels in horsehair. The results showed that free-ranging horses had significantly higher cortisol levels compared to stabled and working horses. This suggests that factors such as management, environment, and behavior may influence the stress levels in horses and should be further investigated.
Article
Engineering, Environmental
Qiuguo Fu, Andreas Scheidegger, Endre Laczko, Juliane Hollender
Summary: The study investigated the toxicokinetic processes and metabolomics effects of the pharmaceutical diclofenac on the freshwater invertebrate Hyalella azteca. Results showed rapid uptake and formation of diclofenac taurine, with slower elimination compared to diclofenac. Metabolomics revealed inhibition of prostaglandin synthesis and impact on the carnitine shuttle pathway at environmentally relevant concentrations.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2021)
Review
Environmental Sciences
Olutobi Daniel Ogunbiyi, Timothy Oladiran Ajiboye, Elizabeth Oyinkansola Omotola, Peter Olusakin Oladoye, Clement Ajibade Olanrewaju, Natalia Quinete
Summary: This review examines the common separation techniques and analytical methodologies for detecting and quantifying PFAS in environmental samples, focusing on their extraction from food items of plant and animal origin. The review highlights recent advances in tandem techniques for improved selectivity and separation of PFAS compounds, and identifies knowledge gaps and research needs in current analytical methodologies.
ENVIRONMENTAL POLLUTION
(2023)
Article
Biotechnology & Applied Microbiology
Reham Alaa Eldin Shaker, Yosra Ibrahim Nagy, Mina E. Adly, Rania Abdelmonem Khattab, Yasser M. Ragab
Summary: In this study, bacterial consortia capable of degrading CIP and LEV were obtained from industrial wastewater. The impacts of critical operating parameters on the degradation of CIP and LEV were investigated, and the possible degradation pathways were suggested by analyzing the intermediate degradation products. This study discovers the ability of previously unreported bacterial strains to degrade both CIP and LEV.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2022)
Review
Infectious Diseases
Silvia Cruz Barros, Ana Sanches Silva, Duarte Torres
Summary: Veterinary drugs are commonly used in disease prevention and treatment. However, the abuse of antibiotics as growth promoters leads to the presence of antibiotic residues in animal-origin foods, posing a threat to food safety and antibiotic resistance. Monitoring the levels of antibiotic residues in food intended for human consumption is crucial for ensuring food safety.
Article
Chemistry, Analytical
R. Sero, M. Vidal, J. Bosch, P. Rodriguez, M. T. Galceran, E. Moyano
Article
Biochemical Research Methods
J. F. Ayala-Cabrera, E. Moyano, F. J. Santos
JOURNAL OF CHROMATOGRAPHY A
(2020)
Article
Chemistry, Applied
Ane Arrizabalaga-Larranaga, Pilar Rodriguez, Mireia Medina, Francisco Javier Santos, Encarnacion Moyano
FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
(2020)
Article
Engineering, Environmental
Noemi Inmaculada Medina-Perez, Manuel Dall'Osto, Stefano Decesari, Marco Paglione, Encarnacion Moyano, Elisa Berdalet
Summary: Critical research is needed to study harmful algal blooms threatening ecosystems and human health, particularly through respiratory routes. This study used a marine aerosol generation tank to investigate the concentrations of toxins synthesized by a natural bloom in both water and air. The results suggest that the production and diffusion of marine toxins in the atmosphere are regulated by complex interactions between biological processes and air-sea aerosol production dynamics.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2021)
Article
Food Science & Technology
Ane Arrizabalaga-Larranaga, Guillem Campmajo, Javier Saurina, Oscar Nunez, Francisco Javier Santos, Encarnacion Moyano
Summary: The study used the UHPLC-APCI-HRMS method for the geographical origin authentication of paprika based on capsaicinoids and carotenoids determination, achieving an authentication rate of 80.9%.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Chemistry, Analytical
Juan F. Ayala-Cabrera, F. Javier Santos, Encarnacion Moyano
Summary: This review article focuses on the latest advances in analytical methodologies for the determination of halogenated organic contaminants, highlighting future prospects for improving analytical determinations of these compounds and the throughput of environmental control laboratories in this field.
TRENDS IN ENVIRONMENTAL ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
J. F. Ayala-Cabrera, M. T. Galceran, E. Moyano, F. J. Santos
Summary: A new GC-HRMS method based on APPI was developed for the accurate determination of SCCPs, achieving efficient ionisation and selective determination by monitoring [M+Cl](-) adduct ions. This method offers advantages such as quantification of individual congener groups and simplification of calibration method, making it a reliable alternative for determining SCCPs in environmental samples.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
A. Arrizabalaga-Larranaga, S. Epigmenio-Chamu, F. J. Santos, E. Moyano
Summary: This paper describes the development of a rapid and sensitive method for detecting banned dyes in spice samples using UHPLC-MS/MS technology combined with three atmospheric pressure ionization sources. The proposed method demonstrated good performance in sample analysis, with low detection limits, good precision, and accurate quantitation.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
R. Sero, J. F. Ayala-Cabrera, F. J. Santos, E. Moyano
Summary: In this work, the combination of paper spray and atmospheric pressure photoionization-mass spectrometry (PS-APPI-MS) was used for the determination of non-polar and low polar compounds. The method demonstrated good performance in the analysis of fluorotelomer alcohols, fluoroctanesulfonamides, and fluorooctane sulfonamido-ethanols, with the ability to detect these compounds at low levels. The PS-APPI-HRMS method allows for fast screening and quantitation of target compounds in waterproof impregnation sprays, providing a cost-effective and efficient approach for routine laboratories.
ANALYTICA CHIMICA ACTA
(2022)
Article
Biochemical Research Methods
Ane Arrizabalaga-Larranaga, Pedro de Juan-de Juan, Claudia Bressan, Mercedes Vazquez-Espinosa, Ana Gonzalez-de-Peredo, F. Javier Santos, Encarnacion Moyano
Summary: This study developed a sensitive and selective UHPLC-MS/MS method for the detection of 23 PAAs, showing the advantage of APCI as an alternative method for the analysis of PAAs. The developed method was applied to the analysis of kitchenware samples, where PAAs concentrations above the legislated limit were detected in 18 out of 20 samples.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Claudia Bressan, Raquel Sero, Elida Alechaga, Nuria Monfort, Encarnacion Moyano, Rosa Ventura
Summary: In this study, desorption electrospray ionization and paper spray ionization coupled with high-resolution mass spectrometry (DESI-HRMS and PSI-HRMS) were used for fast and direct analysis of stimulants and diuretics in urine samples. The PSI-HRMS method performed better for the analysis of stimulants compared to DESI-HRMS, allowing direct analysis of raw urine with better signal-to-noise ratios. However, the results for diuretics were not as satisfactory as expected.
ANALYTICAL METHODS
(2023)
Article
Food Science & Technology
Noemi Inmaculada Medina-Perez, Elena Cerdan-Garcia, Francesc Rubio, Laia Viure, Marta Estrada, Encarnacion Moyano, Elisa Berdalet
Summary: This study aimed to improve the understanding of the nutrient modulation of Ostreopsis cf. ovata toxin content. The total toxin content varied significantly during the natural bloom, with the highest values often coinciding with elevated O. cf. ovata cell abundance and low inorganic nutrient concentrations. The experiments showed that cell toxin content was higher in the stationary phase and in high-nitrogen cultures, with urea inducing lower toxin content than other nutrient sources.
Article
Chemistry, Analytical
Noemi Inmaculada Medina-Perez, Francisco Javier Santos, Elisa Berdalet, Encarnacion Moyano
Summary: This study explores the challenges faced in quantifying analogues of palytoxin (PLTX) using ultra-high-performance liquid chromatography high-resolution mass spectrometry-based techniques. The research focuses on the mass spectra of PLTX analogues and evaluates different instrument conditions and extraction protocols to develop a more reliable and robust quantification method. The proposed method is applied to quantify OVTXs (-a to -g) and iso-PLTX during a 2019 Ostreopsis cf. ovata bloom.
ANALYTICAL METHODS
(2023)
Article
Chemistry, Analytical
N. Medina-Perez, A. Arrizabalaga-Larranaga, R. Sero, E. Moyano
ANALYTICAL METHODS
(2020)
Article
Food Science & Technology
Monica Rosa Loizzo, Paolo Lucci, Oscar Nunez, Rosa Tundis, Michele Balzano, Natale Giuseppe Frega, Lanfranco Conte, Sabrina Moret, Daria Filatova, Encarnacion Moyano, Deborah Pacetti
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
