期刊
ANTIOXIDANTS
卷 11, 期 5, 页码 -出版社
MDPI
DOI: 10.3390/antiox11050935
关键词
hypoxia; vitamin C; Nrf2; Keap1 signaling pathway; oxidative stress; inflammation; apoptosis
资金
- National Natural Science Foundation of China [32122089, U19A2041, 31972805]
- National Key R&D Program of China [2018YFD0900605, 2019YFD0900200]
- Strategic PriorityResearch ProgramofChineseAcademy of Sciences [XDA24010206]
- China Agriculture Research System of MOF [CARS-45-09]
- China Agriculture Research System of MARA [CARS-45-09]
Previous studies have shown that vitamin C has a protective effect in fish. In this study, it was found that vitamin C can attenuate oxidative damage, inflammation, and acute hypoxia-induced apoptosis in gibel carp. This protective mechanism is mediated through the activation of the Nrf2/Keap1 signaling pathway. These findings provide new insights into the defense strategies of fish under hypoxic conditions.
Previous studies have found that vitamin C (VC) has protective effects in fish. However, the efficacy of VC on hypoxia-induced liver injury in fish remains unknown. Therefore, to investigate the protective mechanism of VC on liver injury after acute hypoxic stimulation in fish, gibel carp were fed a diet containing VC for eight weeks, then were subjected to acute hypoxia stimulation. The specific growth rate of fish was increased by the supplementation of VC. Plasma stress markers (glucose, lactic acid, and cortisol) were decreased by the VC supplementation. Moreover, the levels of the inflammatory cytokines (tnf-alpha, il-2, il-6, and il-12) were increased by enhancing the Nrf2/Keap1 signaling pathway. Upregulation of the antioxidant enzymes activity (CAT, SOD, and GPx); T-AOC; and anti-inflammatory factors (il-4 and tgf-beta) highlighted the antioxidant and anti-inflammatory activities of VC. The results showed that VC reduced the apoptotic index of the fish hypothalamus. The expression of GRP78 protein in the liver and endoplasmic reticulum stress and apoptosis induced by hypoxia were inhibited by VC. Taken together, the results indicate that VC can attenuate oxidative damage, inflammation, and acute hypoxia induced apoptosis in gibel carp via the Nrf2/Keap1 signaling pathway. The results identify a new defense strategy of gibel carp in response to hypoxic conditions.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据