Article
Chemistry, Physical
Aysun Aksu, Serap Cetinkaya, Ali Fazil Yeniduenya, Senay Akkus Cetinus, Hayreddin Gezegen, Burak Tuezuen
Summary: This study investigated the immobilization of pectinase on chitosan-alginate-clay beads using epichlorohydrin as a crosslinker. The immobilized enzyme showed improved hydrolytic properties, such as higher optimal temperature and pH. The immobilization process also allowed for reusability of the enzyme, with approximately 45% activity remaining after 5 cycles. Gaussian calculations and molecular docking were performed to further analyze the immobilization product and its effects in human metabolism.
JOURNAL OF MOLECULAR LIQUIDS
(2023)
Article
Agriculture, Multidisciplinary
Uroosa Ejaz, Muhammad Sohail
Summary: Chemical pretreatment of sugarcane bagasse (SB) affects its suitability as a matrix for immobilizing pectinolytic yeast. Untreated SB is more efficient for immobilization, with higher cell retention and pectinase productivity observed over six production cycles.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2021)
Article
Green & Sustainable Science & Technology
Yuanqing Lin, Wenhui Jin, Lixi Cai, Xin Liu, Yue Qiu, Guangya Zhang
Summary: The study proposed a simple and green strategy for multienzyme co-immobilization by integrating ELP-mediated silicification and the spontaneous covalent bond formation of the SC/ST protein pair. This new approach overcomes the complexity and lack of cleanliness in traditional covalent co-immobilization methods, offering a novel pathway for multienzyme co-immobilization.
JOURNAL OF CLEANER PRODUCTION
(2021)
Article
Biochemistry & Molecular Biology
Tayyaba Behram, Sidra Pervez, Muhammad Asif Nawaz, Shujaat Ahmad, Amin Ullah Jan, Haneef Ur Rehman, Shahbaz Ahmad, Nasir Mehmood Khan, Farman Ali Khan
Summary: To enhance the operational stability and reusability of B. subtilis pectinase, it was immobilized on iron oxide nanocarrier. Magnetic iron oxide nanoparticles were synthesized through co-precipitation, and their characteristics were analyzed using SEM and EDEX. Glutaraldehyde was used as a crosslinking agent to immobilize pectinase on the iron oxide nanocarrier. The immobilized pectinase exhibited higher enzymatic activity, improved catalytic stability, and enhanced thermal stability, making it economically viable for industrial use.
Article
Food Science & Technology
Seyed Saeid Hosseini, Faramarz Khodaiyan, Seyed Mohammad E. Mousavi, John F. Kennedy, Seyedeh Zahra Azimi
Summary: In this study, pectinase was immobilized on glass beads using two different cross-linkers: polyaldehyde pullulan and glutaraldehyde. The polyaldehyde pullulan showed better immobilization efficiency and stability compared to glutaraldehyde, while both systems exhibited similar temperature stability. The results suggest that polyaldehyde pullulan could be a promising alternative to glutaraldehyde for enzyme immobilization.
FOOD AND BIOPROCESS TECHNOLOGY
(2021)
Article
Food Science & Technology
Helena de Oliveira Schmidt, Fernanda Camboim Rockett, Giovana Ebert, Giliani Veloso Sartori, Katia Rezzadori, Rafael Costa Rodrigues, Alessandro de Oliveira Rios, Vitor Manfroi
Summary: The study showed that Pectinex (R) enzyme had the best extraction efficiency for feijoa juice, significantly increasing juice yield and positively affecting color parameters. Additionally, microfiltered juice exhibited Newtonian behavior, while fresh juice displayed typical pseudoplastic behavior.
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
(2021)
Review
Chemistry, Multidisciplinary
Yago Araujo Vieira, Danyelle Gurgel, Rosana Oliveira Henriques, Ricardo Antonio Francisco Machado, Debora de Oliveira, Bruno Francisco Oechsler, Agenor Furigo Junior
Summary: The use of laccases in bioremediation processes is being increasingly studied due to the need to address environmental issues caused by emerging contaminants. Immobilized enzymes, especially those using polyacrylonitrile (PAN) as a support, show promising stability and applicability in various industries. Further research is needed to explore different immobilization materials and methods to enhance the efficiency of laccase immobilization for industrial applications.
Review
Food Science & Technology
Zahra Beig Mohammadi, Fuyuan Zhang, Mohammad Saeed Kharazmi, Seid Mahdi Jafari
Summary: This article reviews the application of nanotechnology in the food industry. By immobilizing enzymes on nanomaterials, nanobiocatalysts with improved enzymatic activity, efficiency, stability, and storage stability are created. Nanobiocatalysts have a wide range of applications in food purification, extraction, clarification, production, and packaging, and have been successful in detecting specific components of food contaminants.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2022)
Article
Food Science & Technology
Sara Kharazmi, Asghar Taheri-Kafrani
Summary: An efficient method for enhancing fruit juice quality is achieved by simultaneously hydrolyzing polysaccharides. Covalent co-immobilization of pectinase and xylanase onto functionalized iron oxide nanoparticles is reported as an efficient approach to fabricate a novel nanobiocatalyst for fruit juice clarification. The co-immobilized enzymes showed improved thermal and pH stabilities compared to free counterparts, with more than 74% and 48% of residual activity retained after six consecutive cycles for pectinase and xylanase, respectively. The developed nanobiocatalyst achieved a 53% reduction in turbidity of pineapple juice after 120 minutes treatment, and more than 64% of the initial enzyme activity was retained after nine reuses, indicating its potential application in industrial juice processing.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Kelly Menezes Macedo, Raquel Araujo Azevedo, Erik Galvao Paranhos da Silva, Thiago Pereira das Chagas, Luiz Carlos Salay, Ana Paula Trovatti Uetanabaro, Elizama Aguiar-Oliveira, Andrea Miura da Costa
Summary: Pectinases from Penicillium rolfsii CCMB 714 cultivated in jackfruit seed meal were evaluated for their potential in agroindustrial waste saccharification and orange juice clarification. The results showed that the crude extract of pectinase had optimal activity at pH 3.0-5.0 and 55 degrees C. The saccharification of cocoa husks, mangosteen husks, and passion fruit husks resulted in a significant increase in reducing sugars, and the pectinase also showed potential in clarifying orange juice.
FERMENTATION-BASEL
(2023)
Article
Multidisciplinary Sciences
Diego E. Navarro-Lopez, Alvaro R. Bautista-Ayala, Maria Fernanda Rosales-De la Cruz, Selina Martinez-Beltran, Diego E. Rojas-Torres, A. Sanchez-Martinez, O. Ceballos-Sanchez, J. A. Jauregui-Jauregui, Luis Marcelo Lozano, M. Sepulveda-Villegas, Naveen Tiwari, Edgar R. Lopez-Mena
Summary: This study immobilized pectinase using nanostructured magnetic nanoparticles as supports. The immobilized pectinase showed high retention rate and thermal stability, while the nanoparticles exhibited superparamagnetic behavior, making them an ideal choice for biotechnological applications.
Article
Biochemistry & Molecular Biology
Hao Cheng, Ying-Qin Wei, Ji-Yao Han, Xiao Wang, Wen-Hua Ji, Xing-Hui Jin
Summary: Covalent organic frameworks (COFs) have potential applications in immobilized enzyme fields. A novel COF material, COF-BTDH, was successfully synthesized using 1,3,5-tricarbaldehyde and 3,3'dihydroxybenzidine (BTDH) as organic monomers. The immobilized enzyme showed high activity and stability in catalytic hydrolysis for extracting ginsenosides. The optimized reaction time and material to enzyme ratio were found to be 2 h and 31.25:1, respectively. The immobilized enzyme remained more than 85% active after five consecutive cycles.
PROCESS BIOCHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Zhiyong Guo, Chen Zhang, Tongxu He, Huaiyu Xiao, Jingwen Jin, Qiuhong Yao, Tingxiu Ye, Xi Chen
Summary: This study describes an easy-to-execute strategy to fabricate magnetic heterostructure as multifunctional delivery system. The magnetic heterostructure materials can effectively isolate cytochrome C (Cyt-c) from complex biological samples and improve the immobilization enzyme reactor performance.
Review
Biotechnology & Applied Microbiology
Vivek Chauhan, Diksha Kaushal, Vivek Kumar Dhiman, Shamsher Singh Kanwar, Devendra Singh, Vinay Kumar Dhiman, Himanshu Pandey
Summary: Enzymes are crucial for all organisms and their immobilization can greatly improve the outcome of biocatalytic processes, making the products purer and more economical.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Applied
Gul Ozyilmaz, Ecenur Gunay
Summary: This study investigated the use of co-immobilized amylase, pectinase, and cellulase enzymes for clarifying apple, grape, and pear juices. Four different co-immobilization methods were applied, and the highest activity was observed for enzymes co-immobilized onto silica gel. Response Surface Methodology (RSM) and Design Expert Software were used to optimize the enzyme units, reaction temperature, and time for turbidity and reducing sugar concentration. Batch reactor efficiency was found to be higher compared to the feedback packed bed column reactor. Changes in turbidity, reducing sugar concentrations, and antioxidant activities were observed over time during the interaction of freshly squeezed juices with co-immobilized enzymes.
Review
Medicine, General & Internal
Bruno Sepodes, Joao Rocha, Jorge Batista, Maria-Eduardo Figueira, Frantisek Drafi, Carla Torre
Summary: The introduction of PrEP in Europe has lagged behind the US, leading to limited access and individual expenses. Less than 10% of the population have proper access, and efforts are needed to address cost-effectiveness, target populations, and implementation based on WHO recommendations.
FRONTIERS IN MEDICINE
(2021)
Article
Biotechnology & Applied Microbiology
Fatemeh Izadpanah Qeshmi, Ahmad Homaei, Khosro Khajeh, Ehsan Kamrani, Pedro Fernandes
Summary: This study focused on the cloning, expression, and characterization of L-asparaginase from marine Pseudomonas aeruginosa HR03 isolated from fish intestine. The recombinant enzyme HR03Asnase showed high similarity to type I L-asparaginase from Pseudomonas aeruginosa. It exhibited a wide range of activity and had potential applications in pharmaceutical and food industries.
MARINE BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Larissa Emanuelle da Silva Almeida, Pedro Fernandes, Sandra Aparecida de Assis
Summary: This review provides information on different techniques and supports that have been studied for the immobilization of beta-glucosidase, as well as the application of these immobilized systems in commercial mixtures. Immobilization of the enzyme can improve its catalytic activity, operational and storage stabilities, and enhance its economic viability in industrial production.
Review
Medicine, General & Internal
Maria Manuel Teixeira, Fabio Cardoso Borges, Paula Sousa Ferreira, Joao Rocha, Bruno Sepodes, Carla Torre
Summary: This study analyzed the evidence based on PRO as part of new oncology indications receiving positive opinions issued by the European Medicines Agency between 2017 and 2020, and found that PRO related label claims were granted only in a small percentage of indications.
FRONTIERS IN MEDICINE
(2022)
Article
Health Care Sciences & Services
Ines Martinho, Antonio Teixeira Rodrigues, Jose Guerreiro, Joao Rocha, Bruno Sepodes, Carla Torre
Summary: Comparative drug utilization studies help to understand and compare prescribing patterns, contributing to efficient and safe use of medicines. This study compared consumption patterns of high expenditure therapeutic groups between Portugal and six European countries, and simulated cost-saving scenarios based on different prescribing patterns. Findings showed that despite having the lowest GDP per capita, Portugal had the highest uptake of costly drugs.
EXPERT REVIEW OF PHARMACOECONOMICS & OUTCOMES RESEARCH
(2023)
Article
Chemistry, Multidisciplinary
Muhammad Bilal, Ehsan Ullah Rashid, Jakub Zdarta, Jose C. S. dos Santos, Pedro C. B. Fernandes, Hairong Cheng, Teofil Jesionowski
Summary: This paper provides an overview of the development and application of multifunctional magnetic nanobiocatalytic systems. Magnetic nanomaterials, with their substantial surface area and high mass transferring ability, have been widely used as support matrices for enzyme immobilization. Magnetic nanoparticles incorporated biocatalysts exhibit broad-working temperature and pH profiles and enhanced storage and thermal stabilities. The potential applications of magnetic nanobiocatalytic systems in various industrial sectors are comprehensively explored, including wastewater treatment, biodiesel and butanol production, hydrolysis of lignocellulosic biomass, glucose monitoring, fruit juice extraction and clarification, and synthesis of non-natural benzylisoquinoline alkaloid.
SUSTAINABLE CHEMISTRY AND PHARMACY
(2022)
Review
Biochemistry & Molecular Biology
Azadeh Beygmoradi, Ahmad Homaei, Roohullah Hemmati, Pedro Fernandes
Summary: Protein folding is a process that determines the three-dimensional structure of a protein. Recombinant protein production can face problems like proteolysis, incorrect folding, and protein aggregation. Identifying proper protein refolding conditions is challenging, and molecular chaperones can help in proper folding. Proper protein folding is essential for post-translational modifications, and incorrect folding can lead to cellular damage. Understanding recombinant protein expression and addressing folding issues are important.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Review
Chemistry, Physical
Pedro Sousa Sampaio, Pedro Fernandes
Summary: Biocatalysis is an effective and sustainable method for producing a wide range of compounds. Enzyme-related knowledge has increased greatly, and computational approaches, such as machine learning methods, have become relevant in biocatalysis. This review provides insights into the contribution of biocatalysis in biochemical engineering and bioprocesses, and highlights the key aspects of machine learning methods used in biocatalysis. Several illustrative examples and future developments are also presented.
Review
Medicine, General & Internal
Adriana Marques, Carla Torre, Rui Pinto, Bruno Sepodes, Joao Rocha
Summary: Sepsis is a life-threatening condition caused by a dysregulated host response to infection, affecting over 25 million people annually. Septic shock, a severe subset of sepsis, is characterized by persistent low blood pressure, with hospital mortality rates exceeding 40%. Despite advances in early sepsis mortality, long-term complications such as secondary infection remain a significant cause of death, and there are currently no sepsis-specific therapies. Immunostimulatory therapy, including cytokines, growth factors, immune checkpoint inhibitors, and cellular therapies, holds promise in addressing this unmet medical need.
JOURNAL OF CLINICAL MEDICINE
(2023)
Editorial Material
Engineering, Chemical
Pedro Fernandes, Carla C. C. R. de Carvalho
Review
Health Policy & Services
Jorge P. B. Batista, Carla Torre, Jose Manuel Sousa Lobo, Bruno Sepodes
Summary: This study analyzed the educational activities submitted for accreditation from 2009 to 2019. The results showed an increase in the number of accredited activities over the past decade (52.6%). The majority of the activities were accredited (98.9%). The main promoters of these activities were pharmacy sectoral associations (29.6%), consultancy/training companies (19.6%), the PPS (18.5%), pharmaceutical industry (17.7%), and wholesalers' consortia (9.0%). Academia only represented a small proportion of the activities (2.3%). The most common topics were pharmacology & pharmacotherapy (9.9%), counselling (9.8%), and management & administration (7.2%). Face-to-face trainings (68.9%) and e-learning trainings (13.1%) were the most accredited activity types.
HUMAN RESOURCES FOR HEALTH
(2022)