Review
Chemistry, Multidisciplinary
Yi Xie, Huimin Li, Fumin Chen, Srisruthi Udayakumar, Khyati Arora, Hui Chen, Yang Lan, Qinqin Hu, Xiaonong Zhou, Xiaokui Guo, Leshan Xiu, Kun Yin
Summary: Mitigating the spread of global infectious diseases requires rapid and accurate diagnostic tools. This review highlights the recent advances in CRISPR-based microfluidic systems for infectious diseases diagnosis, including detection mechanisms, microfluidic devices, and improvement strategies.
Article
Veterinary Sciences
Hung-Yueh Yeh, Amal Awad, Michael J. Rothrock
Summary: The study explored the diversity of CRISPR sequences in C. jejuni isolates from an animal farm, revealing various combinations of CRISPR sequence lengths and spacer numbers. The results indicated that CRISPR genotyping combined with MLST methods could discriminate C. jejuni isolates, highlighting the need for further research on the application of CRISPR in outbreaks.
VETERINARY MEDICINE AND SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Jen-Hui Tsou, Hongjie Liu, Sanford A. Stass, Feng Jiang
Summary: This study developed a rapid CRISPR test for sensitive detection of SARS-CoV-2 without the need for RNA extraction, allowing for completion within one hour. The test is simple to operate, requiring minimal equipment, and results can be visualized immediately using a UV light illuminator or paper strips.
Article
Chemistry, Multidisciplinary
Yuzhi Chen, Zhi Chen, Tianzhong Li, Meng Qiu, Jinghan Zhang, Yan Wang, Wu Yuan, Aaron Ho-Pui Ho, Omar Al-Hartomy, Swelm Wageh, Abdullah G. Al-Sehemi, Xin Shi, Jingfeng Li, Zhongjian Xie, Li Xuejin, Han Zhang
Summary: This study presents a CRISPR/Cas12a-based diagnostic platform with a surface plasmon resonance-based fiber tip (CRISPR-SPR-FT) biosensor for detecting the mpox virus. The compact CRISPR-SPR-FT biosensor offers high stability and portability, and can analyze mpox viral DNA without amplification in under 1.5 hours with a limit of detection below 5 aM.
Article
Chemistry, Multidisciplinary
Cheng-Yu Li, Bei Zheng, Jiang-Tao Li, Jia-Ling Gao, Yu-Heng Liu, Dai-Wen Pang, Hong-Wu Tang
Summary: The CRISPR/Cas12a system offers an alternative opportunity for designing fluorescence biosensors with outstanding precision in target recognition. By utilizing collateral cleavage and representative transduction pathways, the system can extend sensing coverage and improve assay sensitivity. The developed biosensors not only exhibit ultrasensitive behavior towards non-nucleic acid analytes but also show practical application capacity in real samples.
Review
Behavioral Sciences
Arshad Mehmood, Wajid Ali, Zaheer Ud Din, Shuang Song, Muhammad Sohail, Wahid Shah, Jiangyuan Guo, Ruo-Yi Guo, Ikram Ilahi, Suleman Shah, Fadhl Al-Shaebi, Liaqat Zeb, Ernest Amponsah Asiamah, Zaid Al-Dhamin, Hazrat Bilal, Bin Li
Summary: Genome-editing technology such as CRISPR/Cas9 has bridged the translational gap in gene therapy treatments, particularly in the experimental and therapeutic applications for central nervous system diseases. Studies have identified genetic mutations and duplications related to Parkinson's disease pathogenesis, suggesting that gene editing through CRISPR technology could be beneficial for PD treatment. This highlights the importance and potential of CRISPR technology in research.
BRAIN AND BEHAVIOR
(2021)
Article
Biology
Marco Tatullo, Luisa Limongelli, Rosa maria Marano, Lessandra Valletta, Ngela Tempesta, Andro Rengo
Summary: The CRISPR-Cas9 system is a biotechnology that improves the efficacy of traditional treatments, particularly in the field of oral medicine. By speeding up and customizing therapies, this technology enhances the predictability of clinical outcomes and opens up new possibilities for treating oral diseases.
Article
Chemistry, Multidisciplinary
Huahua Yue, Mengqi Huang, Tian Tian, Erhu Xiong, Xiaoming Zhou
Summary: CRISPR-based diagnoses, derived from gene-editing technology, have unique features such as recognition at physiological temperature, excellent specificity, and high-efficiency signal amplification capabilities. Micro/nanotechnologies play key roles in the advancement of CRISPR diagnosis, including the use of nanoprobes, nanochips, microfluidic technology, and lateral flow strips, leading to new opportunities and improvements such as equipment-free detection, more compact detection systems, improved sensitivity, and quantitative capabilities. Despite significant progress, CRISPR diagnosis has not yet reached its full potential, with upcoming opportunities and improvements expected to be driven by micro/nanotechnologies.
Review
Chemistry, Multidisciplinary
Mengdi Bao, Qun Chen, Zhiheng Xu, Erik C. Jensen, Changyue Liu, Jacob T. Waitkus, Xi Yuan, Qian He, Peiwu Qin, Ke Du
Summary: CRISPR, with its unique properties, has emerged as a powerful molecular biosensing tool for nucleic acids and biomarkers. However, several key challenges need to be addressed for it to become a new gold standard. This paper reviews the history of biosensors, discusses current challenges and recent breakthroughs in CRISPR-based nucleic acid detection, and focuses on future advancements needed for CRISPR-based molecular biosensors.
Article
Biotechnology & Applied Microbiology
Cheng Yang, Wangfeng Shao, Lingling Wei, Lingxiao Chen, Aihua Zhu, Zhiming Pan
Summary: Salmonella as a zoonotic pathogen, especially in transmission between household pets and humans, has attracted worldwide attention. A study conducted in Xuzhou, Jiangsu Province, China, investigated the distribution of dog Salmonella from pet hospitals and breeding base. The results showed that S. Kentucky was the most prevalent serotype, and CRISPR genes have better recognition ability for the same serotype.
Review
Plant Sciences
Yuchun Rao, Xi Yang, Chenyang Pan, Chun Wang, Kejian Wang
Summary: CRISPR-Cas9 is a promising gene editing technology with the advantages of simplicity, high efficiency, high specificity, and low cost. This review systematically summarizes its generation, advantages, and three derivative technologies, as well as its applications in crop improvement.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Chemistry, Physical
Weiran Su, Junru Li, Chen Ji, Congshuo Chen, Yuzheng Wang, Huili Dai, Fengqin Li, Peifeng Liu
Summary: CRISPR/Cas systems are powerful tools for disease biomarkers detection, capable of detecting nucleic acid and non-nucleic acid targets. This review summarizes the principles and characteristics of various CRISPR/Cas systems, and introduces the applications of CRISPR/Cas systems in detecting nucleic and non-nucleic acid targets. The prospects and challenges of their applications in biosensing are also discussed.
Article
Microbiology
Harumi Mizuki, Yu Shimoyama, Taichi Ishikawa, Minoru Sasaki
Summary: This study analyzed the presence of CRISPR/Cas systems in five Mycoplasma salivarium strains and found that only ATCC 29803 possessed a complete CRISPR/Cas system. The cas9 gene of this strain was not disrupted by stop codons and potentially functions as an RNA-guided endonuclease.
JOURNAL OF ORAL MICROBIOLOGY
(2022)
Article
Microbiology
Thibault Le Gratiet, Caroline Le Marechal, Marie Devaere, Marianne Chemaly, Cedric Woudstra
Summary: Classified as the genospecies Clostridium novyi sensu lato and distributed into four lineages, Clostridium botulinum, Clostridium novyi, and Clostridium haemolyticum are clostridial pathogens causing animal diseases. This study investigated the CRISPR-Cas systems in 58 Clostridium novyi sensu lato genomes, revealing different types of systems and their association with mobilome composition and dynamics in the species. The presence of active gene exchanges within the species was confirmed based on the analysis of CRISPR arrays and spacers.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Immunology
Song Wang, Jiayi Hu, Chuanying Sui, Guangliang He, Zihan Qu, Xiaofei Chen, Yashan Wang, Dingjie Guo, Xin Liu
Summary: This meta-analysis showed that CRISPR has a diagnostic performance close to the gold standard in detecting COVID-19, and it is expected to meet the on-site testing requirements in resource-poor areas.
MICROBIAL PATHOGENESIS
(2022)
Article
Chemistry, Physical
Yongjun Ji, Xiaoli Chen, Shaomian Liu, Liwen Xing, Xingyu Jiang, Bin Zhang, Huifang Li, Wenxing Chen, Ziyi Zhong, Ligen Wang, Guangwen Xu, Fabing Su
Summary: This study demonstrates a facile strategy to enhance the catalytic performance of CuO-ZnO catalyst by introducing defects such as oxygen vacancies and localized amorphous regions. Theoretical calculations reveal the influence of defects on the generation of active phases and electronic structures, providing new insights for improving catalyst structure and catalytic performance.
Article
Materials Science, Multidisciplinary
Tianzhong Li, Jinyong Wang, Tongkai Chen, Swelm Wageh, Ahmed A. Al-Ghamdi, Jiangtian Yu, Zhongjian Xie, Han Zhang
Summary: In this study, a drug formulation based on lactic acid bacteria (LAB) was developed to produce reactive oxygen species (ROS) in tumors and induce host immunity. The LAB were modified with zeolitic imidazolate framework-67 (ZIF-67) and effectively delivered into tumor cells, leading to the generation of ROS upon light exposure. The bacterial hybrid induced tumor shrinkage through photodynamic therapy and stimulated inflammation to attract immune cells for cancer elimination.
SCIENCE CHINA-MATERIALS
(2023)
Review
Chemistry, Multidisciplinary
Max J. Hulsey, Sikai Wang, Bin Zhang, Shipeng Ding, Ning Yan
Summary: Single-atom catalysts offer unique advantages but are often intrinsically ill-defined. In this Account, we describe approaches to break down the complexity of supported single-atom catalysts through the careful choice of oxide supports with specific binding motives as well as the adsorption of well-defined ligands. We utilized polyoxometalate-supported single-atom catalysts as ideal systems for the in situ spectroscopic study of single atom sites during reactions.
ACCOUNTS OF CHEMICAL RESEARCH
(2023)
Article
Chemistry, Physical
Yan Wang, Xueyang Fang, Junqing Zeng, Shuang Li, Xin Wang, Bin Zhang
Summary: The study developed a composite photocatalyst consisting of 2D layered Ti3C2, thiolate-protected Au clusters, and TiO2 nanoparticles. The introduced Ti3C2 served as an intermedium for electron transfer and significantly improved the charge transfer efficiency. The resulting Au/Ti3C2/TiO2 photocatalyst exhibited excellent performance for H-2 production, with a rate 13.7 times higher than Au/TiO2. The strong interaction between Ti3C2 and Au/TiO2 also contributed to the high stability and durability of the composite material.
APPLIED SURFACE SCIENCE
(2023)
Article
Engineering, Environmental
Chenyang Xing, Zihao Li, Shaohui Zhang, Jian Bang, Zhongjian Xie, Han Zhang, Zhengchun Peng
Summary: In order to address global freshwater scarcities, innovative materials are needed for the development of solar-powered interfacial desalination and purification technologies. This study utilizes polymeric phase inversion to develop PVA-based foam hydrogels with high porosity, compressibility, and rapid water transport. These materials also exhibit low water evaporation enthalpies and achieve high evaporation rates and energy efficiency under 1 sun irradiation.
CHEMICAL ENGINEERING JOURNAL
(2023)
Review
Chemistry, Physical
Xiaohan Duan, Zhihao Liu, Zhongjian Xie, Ayesha Khan Tareen, Karim Khan, Bin Zhang, Han Zhang
Summary: Currently, numerous monoelemental two-dimensional materials, called Xenes, have been extensively studied and have shown great potential for applications in photoelectric and biomedicine fields. These materials have unique structural characteristics and excellent properties, including high optical response capability and large specific surface area. In particular, Xenes have diverse utilization as biosensors for nucleic acid sequencing, bioactive detection, and cancer diagnosis.
Article
Chemistry, Analytical
Duo Yi, Bin Zhang, Youfu Geng, Xuejin Li
Summary: This study proposes a TiN-modified fiber optic interferometer, which greatly enhances the refractive index (RI) response by utilizing the unique properties of TiN. The deposited TiN nanoparticles enhance the evanescent field excitation and modulate the effective RI difference, resulting in enhanced RI responses. Sensing performances can be flexibly adapted based on different detection requirements, making the TiN-sensitized fiber optic interferometer potentially applied for high-sensitive biosensing applications.
Review
Chemistry, Multidisciplinary
Karim Khan, Ayesha Khan Tareen, Muhammad Iqbal, Zhang Ye, Zhongjian Xie, Asif Mahmood, Nasir Mahmood, Han Zhang
Summary: MXenes are a novel and extensive class of 2D materials family, which consist of early transition metals based 2D carbides, nitrides and carbonitrides nanomaterials. MXenes possess distinctive surface chemistry, graphene like shape, metallic conductivity, high hydrophilicity, outstanding mechanical and thermal properties, redox capacity and are affordable with mass-produced nature, making them of tremendous scientific and technological significance.
Article
Biotechnology & Applied Microbiology
Zhi Chen, Chenshuo Wu, Yuxuan Yuan, Zhongjian Xie, Tianzhong Li, Hao Huang, Shuang Li, Jiefeng Deng, Huiling Lin, Zhe Shi, Chaozhou Li, Yabin Hao, Yuxuan Tang, Yuehua You, Omar A. Al-Hartomy, Swelm Wageh, Abdullah G. Al-Sehemi, Ruitao Lu, Ling Zhang, Xuechun Lin, Yaqing He, Guojun Zhao, Defa Li, Han Zhang
Summary: Since the end of 2019, a highly contagious disease called COVID-19, caused by SARS-CoV-2, has claimed numerous lives worldwide. The latest variant of concern is omicron, and BA.5 is becoming the dominant subtype, replacing the BA.2 variant. These subtypes have an L452R mutation, which increases their transmissibility among vaccinated individuals. Current methods for identifying SARS-CoV-2 variants rely on time-consuming and costly PCR-based gene sequencing. In this study, researchers developed a rapid and ultrasensitive electrochemical biosensor using MXene-AuNP electrodes and the CRISPR/Cas13a system to detect the L452R mutation in RNA samples, enabling early diagnosis and distinguishing omicron BA.5 and BA.2 variants.
JOURNAL OF NANOBIOTECHNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Yuzhi Chen, Zhi Chen, Tianzhong Li, Meng Qiu, Jinghan Zhang, Yan Wang, Wu Yuan, Aaron Ho-Pui Ho, Omar Al-Hartomy, Swelm Wageh, Abdullah G. Al-Sehemi, Xin Shi, Jingfeng Li, Zhongjian Xie, Li Xuejin, Han Zhang
Summary: This study presents a CRISPR/Cas12a-based diagnostic platform with a surface plasmon resonance-based fiber tip (CRISPR-SPR-FT) biosensor for detecting the mpox virus. The compact CRISPR-SPR-FT biosensor offers high stability and portability, and can analyze mpox viral DNA without amplification in under 1.5 hours with a limit of detection below 5 aM.
Article
Chemistry, Analytical
Lude Wang, Feicui Xu, Chen Zhang, Swelm Wageh, Omar A. A. Al-Hartomy, Bin Zhang, Tingqiang Yang, Han Zhang
Summary: Transition metal dichalcogenides (TMDs) have great potential for chemiresistive gas sensors. However, TMD alloys with tunable electronic structures have not been extensively studied in gas sensing. In this study, Mo0.5W0.5S2 alloy nanoparticles (NPs) were prepared and tested for ammonia sensing. The results showed that the Mo0.5W0.5S2 NPs exhibited good response to ammonia with a low detection limit of 500 ppb. The sensor also demonstrated excellent stability and selectivity to ammonia in the presence of interferences.
Article
Chemistry, Physical
Xuewei Wang, Bin Zhang, Xi Chen
Summary: Lignocellulosic biomass refining is an important alternative to fossil refining for sustainable development. However, the complexity of biomass brings challenges in achieving high selectivity. Single-atom catalysts have shown great potential in biomass refining, especially for selective hydrogenation reactions. This perspective provides a concise account of recent progress in hydrogenation reactions of lignocellulosic biomass and its derived feedstocks using single-atom catalysts, including catalyst structure, reaction conditions, and mechanism aspects according to different substrate species (furfural, 5-hydroxymethyl furfural, etc.).
SUSTAINABLE ENERGY & FUELS
(2023)
Article
Optics
Jiajie Chen, Zhi Chen, Changle Meng, Jianxing Zhou, Yuhang Peng, Xiaoqi Dai, Jingfeng Li, Yili Zhong, Xiaolin Chen, Wu Yuan, Ho-Pui Ho, Bruce Zhi Gao, Junle Qu, Xueji Zhang, Han Zhang, Yonghong Shao
Summary: Optothermal nanotweezers have been developed as an innovative optical manipulation technique that combines optothermal manipulation and CRISPR-based bio-detection. This innovative approach endows optical tweezers with the ability to identify DNA in aqueous solutions.
LIGHT-SCIENCE & APPLICATIONS
(2023)
Article
Chemistry, Physical
Yongjun Ji, Xiaoli Chen, Shaomian Liu, Liwen Xing, Xingyu Jiang, Bin Zhang, Huifang Li, Wenxing Chen, Ziyi Zhong, Ligen Wang, Guangwen Xu, Fabing Su
Summary: This study demonstrates a simple strategy to improve the catalytic performance of CuO-ZnO model catalysts by introducing two different types of defects. Oxygen vacancies primarily enhance the selectivity of the target product, while localized amorphous regions significantly increase the conversion of the reactants.