Article
Cell Biology
Margret A. Fye, Irina Kaverina
Summary: Pancreatic beta cells secrete insulin to maintain glucose homeostasis and prevent diseases like diabetes. Insulin secretion is efficiently achieved by clustering secretion events at the cell membrane, forming insulin secretion hot spots. Proteins associated with the cytoskeleton have been found to localize and function at these hot spots. Microtubules and F-actin are involved in regulating these hot spot proteins and their role in secretion, and mechanical regulation may also play a role.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Ajay P. Nayak, John M. Lim, Eylon Arbel, Ruping Wang, Dominic R. Villalba, Tahn L. Nguyen, Niccole Schaible, Ramaswamy Krishnan, Dale D. Tang, Raymond B. Penn
Summary: Current therapeutic approaches for bronchoconstriction primarily rely on beta-agonists to regulate airway smooth muscle contraction, while targeting actin cytoskeleton polymerization in the smooth muscle represents an alternative means of regulation. The combination of beta-agonists and inhibitors of Abl1 or c-Abl can exert additive effects on preventing or reversing smooth muscle contraction, suggesting a potential therapeutic strategy for managing airway resistance.
Article
Multidisciplinary Sciences
Ruiling Liu, Cuilian Liu, Xiaozhen He, Peng Sun, Bin Zhang, Haoran Yang, Weiyun Shi, Qingguo Ruan
Summary: In this study, researchers investigate the role of miR-21 in the regulation of pancreatic beta cell function. They find that miR-21 promotes glucose-stimulated insulin secretion by enhancing glucose uptake and up-regulating Glut2 expression. They also demonstrate that delivering miR-21 into the pancreas can decrease blood glucose levels in diabetic mice.
NATURE COMMUNICATIONS
(2022)
Article
Dentistry, Oral Surgery & Medicine
Xiaoming Wang, Weilong Liu, Xiao Luo, Qian Zheng, Bing Shi, Renkai Liu, Chenghao Li
Summary: In this study, we investigated the exact mechanism of beta-catenin/F-actin in regulating palatal reorientation. We found that overexpression of beta-catenin disrupted F-actin responsible for cytoskeletal remodeling in palatal mesenchymal cells, leading to failed palatal elevation and visible cleft palate. Furthermore, we identified alpha-actinin-4 as a key mediator in the regulation of F-actin cytoskeleton reorganization by beta-catenin.
Article
Multidisciplinary Sciences
Keiichi Katsumoto, Siham Yennek, Chunguang Chen, Luis Fernando Delgadillo Silva, Sofia Traikov, Dror Sever, Ajuna Azad, Jingdong Shan, Seppo Vainio, Nikolay Ninov, Stephan Speier, Anne Grapin-Botton
Summary: Diabetes is a disease characterized by the loss or dysfunction of pancreatic beta-cells. This study reveals that WNT4 is activated heterogeneously by beta-cells during their postnatal maturation, with WNT4-positive cells being more mature. Inactivating WNT4 in beta-cells after maturation impairs beta-cell function.
NATURE COMMUNICATIONS
(2022)
Article
Immunology
Kaylyn R. Scanlon, Gabrielle Keb, Katerina Wolf, Travis J. Jewett, Kenneth A. Fields
Summary: Chlamydia trachomatis actively promotes invasion of epithelial cells through a type III secretion system and at least four effectors. TmeA directly activates host N-WASP to stimulate actin polymerization, while TmeB interferes with invasion. TmeA is dispensable for invasion in the absence of TmeB, and TmeB can interfere with Arp2/3-mediated actin polymerization.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Cell Biology
Hiroshi Tokuo, Shigeru Komaba, Lynne M. Coluccio
Summary: The study reveals that Myo1b plays a crucial role in mediating the early stage of insulin granule trafficking from the trans-Golgi network in pancreatic beta-cells.
MOLECULAR BIOLOGY OF THE CELL
(2021)
Article
Biochemistry & Molecular Biology
Siresha Bathina, Tumininu S. Faniyan, Lauren Bainbridge, Autumn Davis, Kavaljit H. Chhabra
Summary: The role of beta-cell Glut2 in regulating insulin secretion and blood glucose levels in mice was investigated in this study. Knocking down Glut2 specifically in beta-cells did not affect glucose-stimulated insulin secretion and systemic glucose homeostasis in mice.
Article
Endocrinology & Metabolism
Jinghe Li, Ryota Inoue, Yu Togashi, Tomoko Okuyama, Aoi Satoh, Mayu Kyohara, Kuniyuki Nishiyama, Takahiro Tsuno, Daisuke Miyashita, Tatsuya Kin, A. M. James Shapiro, Resilind Su Ern Chew, Adrian Kee Keong Teo, Seiichi Oyadomari, Yasuo Terauchi, Jun Shirakawa
Summary: Imeglimin protects beta-cell survival by enhancing function, promoting proliferation, and preventing apoptosis, thereby improving islet survival and mass.
Review
Plant Sciences
Yi-Qun Gao, Dai-Yin Chao
Summary: This review summarizes recent advances in the study of how vesicle trafficking regulates nutrient homeostasis in plants and discusses new perspectives on future studies, which may inspire researchers to explore new approaches to improve human diet and health.
Article
Biochemistry & Molecular Biology
Waruni C. Dissanayake, Peter R. Shepherd
Summary: This study demonstrates the importance of adherens junctions in insulin release and identifies the role of p120 catenin in regulating this process.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Endocrinology & Metabolism
Yi Cheng, Xin-Ming Yao, Si-Min Zhou, Yue Sun, Xiang-Jian Meng, Yong Wang, Yu-Jie Xing, Shu-Jun Wan, Qiang Hua
Summary: The study demonstrates that METTL3 improves methylglyoxal-induced impairment of insulin secretion in pancreatic beta cells by regulating MafA expression.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Plant Sciences
Jianyan Zeng, Jing Xi, Baoxia Li, Xingying Yan, Yonglu Dai, Yiping Wu, Yuehua Xiao, Yan Pei, Mi Zhang
Summary: This study elucidates the roles of actin and microtubule cytoskeletons in the initiation of cotton fiber growth. Both cytoskeletons align along the growth axis in initiating fibers. Dense and bundled actin filaments (AFs) are observed in fiber cells, while microtubules (MTs) are randomized. Pharmacological experiments demonstrate that depolymerization of both AFs and MTs assist fiber initiation, while AF stabilization and depolymerization inhibit elongation. Notably, the organization of AFs is correlated with MT dynamics.
PLANT CELL REPORTS
(2022)
Article
Cell Biology
Hong Gao, Zhenlong Luo, Zhongmou Jin, Yudong Ji, Wei Ying
Summary: The study revealed that ATM EVs from obese mice play a significant role in inhibiting insulin secretion and promoting beta cell proliferation, with miR-155 identified as a highly enriched miRNA in obese ATM EVs and exerting critical regulatory effects on beta cell responses.
Article
Biochemistry & Molecular Biology
Seiya Asai, Jana Moravcova, Lenka Zakova, Irena Selicharova, Romana Hadravova, Andrzej Marek Brzozowski, Jiri Novacek, Jiri Jiracek
Summary: We found two different rhomboidal crystal lattices in the insulin secretory granules, suggesting the existence of microcrystalline insulin forms, possibly consisting of 4Zn2+-hexamers of this hormone.
Correction
Cell Biology
Anthony J. Kee, Lingyan Yang, Christine A. Lucas, Michael J. Greenberg, Nick Martel, Gary M. Leong, William E. Hughes, Gregory J. Cooney, David E. James, E. Michael Ostap, Weiping Han, Peter W. Gunning, Edna C. Hardeman
Article
Cell Biology
Anthony J. Kee, Lingyan Yang, Christine A. Lucas, Michael J. Greenberg, Nick Martel, Gary M. Leong, William E. Hughes, Gregory J. Cooney, David E. James, E. Michael Ostap, Weiping Han, Peter W. Gunning, Edna C. Hardeman
Article
Endocrinology & Metabolism
Bing M. Liao, Sophie A. McManus, William E. Hughes, Carsten Schmitz-Peiffer
MOLECULAR ENDOCRINOLOGY
(2016)
Article
Multidisciplinary Sciences
Anne Karine Lagendijk, Guillermo A. Gomez, Sungmin Baek, Daniel Hesselson, William E. Hughes, Scott Paterson, Daniel E. Conway, Heinz-Georg Belting, Markus Affolter, Kelly A. Smith, Martin A. Schwartz, Alpha S. Yap, Benjamin M. Hogan
NATURE COMMUNICATIONS
(2017)
Article
Multidisciplinary Sciences
Christoph Jandl, Sue M. Liu, Pablo F. Canete, Joanna Warren, William E. Hughes, Alexis Vogelzang, Kylie Webster, Maria E. Craig, Gulbu Uzel, Alexander Dent, Polina Stepensky, Barbel Keller, Klaus Warnatz, Jonathan Sprent, Cecile King
NATURE COMMUNICATIONS
(2017)
Article
Cell Biology
Martin Whitham, Benjamin L. Parker, Martin Friedrichsen, Janne R. Hingst, Marit Hjorth, William E. Hughes, Casey L. Egan, Lena Cron, Kevin I. Watt, Rhiannon P. Kuchel, Navind Jayasooriah, Emma Estevez, Tim Petzold, Catherine M. Suter, Paul Gregorevic, Bente Kiens, Erik A. Richter, David E. James, Jorgen F. P. Wojtaszewski, Mark A. Febbraio
Article
Oncology
Waruni C. Dissanayake, Brie Sorrenson, Emmanuelle Cognard, William E. Hughes, Peter R. Shepherd
EXPERIMENTAL CELL RESEARCH
(2018)
Article
Chemistry, Multidisciplinary
Mahdi Zeraati, David B. Langley, Peter Schofield, Aaron L. Moye, Romain Rouet, William E. Hughes, Tracy M. Bryan, Marcel E. Dinger, Daniel Christ
Article
Biochemistry & Molecular Biology
James W. Robinson, Iryna Leshchyns'ka, Hovik Farghaian, William E. Hughes, Vladimir Sytnyk, Graham G. Neely, Adam R. Cole
BIOCHEMICAL JOURNAL
(2014)
Review
Oncology
David Herrmann, James R. W. Conway, Claire Vennin, Astrid Magenau, William E. Hughes, Jennifer P. Morton, Paul Timpson
Article
Endocrinology & Metabolism
Martin Whitham, Martin Pal, Tim Petzold, Marit Hjorth, Casey L. Egan, Julia S. Brunner, Emma Estevez, Peter Iliades, Borivoj Zivanovic, Saskia Reibe, William E. Hughes, Maria Findeisen, Juan Hidalgo, Mark A. Febbraio
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
(2019)
Article
Multidisciplinary Sciences
Chenxu Yan, Tianshu Zeng, Kailun Lee, Max Nobis, Kim Loh, Luoning Gou, Zefeng Xia, Zhongmin Gao, Mohammed Bensellam, Will Hughes, Jackie Lau, Lei Zhang, Chi Kin Ip, Ronaldo Enriquez, Hanyu Gao, Qiao-Ping Wang, Qi Wu, Jody J. Haigh, D. Ross Laybutt, Paul Timpson, Herbert Herzog, Yan-Chuan Shi
Summary: Blocking Y1 receptors in peripheral tissues using selective antagonists can reduce weight gain, decrease fat mass, and improve glucose metabolism, suggesting a potential safer and more effective treatment for diet-induced obesity.
NATURE COMMUNICATIONS
(2021)
Article
Biology
Alvaro Gonzalez Rajal, Kamila A. Marzec, Rachael A. McCloy, Max Nobis, Venessa Chin, Jordan F. Hastings, Kaitao Lai, Marina Kennerson, William E. Hughes, Vijesh Vaghjiani, Paul Timpson, Jason E. Cain, D. Neil Watkins, David R. Croucher, Andrew Burgess
Summary: The study identified a non-genetic mechanism of resistance in human lung adenocarcinoma that is dependent on the cell cycle stage at the time of cisplatin exposure, using RNA sequencing and biosensors to track single-cell fates.