Germ granules and gene regulation in the Caenorhabditis elegans germline

ZSP‐1 is a Z granule surface protein required for Z granule fluidity and germline immortality in Caenorhabditis elegans

(2021) Gang Wan et al.   EMBO JOURNAL

C. elegans germ granules require both assembly and localized regulators for mRNA repression

(2021) Scott Takeo Aoki et al.   Nature Communications

Germ granule dysfunction is a hallmark and mirror of Piwi mutant sterility

(2021) Maya Spichal et al.   Nature Communications

Germline inherited small RNAs facilitate the clearance of untranslated maternal mRNAs in C. elegans embryos

(2021) Piergiuseppe Quarato et al.   Nature Communications

piRNAs coordinate poly(UG) tailing to prevent aberrant and perpetual gene silencing

(2021) Aditi Shukla et al.   CURRENT BIOLOGY

An essential role for the piRNA pathway in regulating the ribosomal RNA pool in C. elegans

(2021) Lamia Wahba et al.   DEVELOPMENTAL CELL

Structural basis of PETISCO complex assembly during piRNA biogenesis in C. elegans

(2021) Cecilia Perez-Borrajero et al.   GENES & DEVELOPMENT

Two isoforms of the essential C. elegans Argonaute CSR-1 differentially regulate sperm and oocyte fertility

(2021) Amanda G Charlesworth et al.   NUCLEIC ACIDS RESEARCH

Translation and codon usage regulate Argonaute slicer activity to trigger small RNA biogenesis

(2021) Meetali Singh et al.   Nature Communications

Arginine methylation promotes siRNA-binding specificity for a spermatogenesis-specific isoform of the Argonaute protein CSR-1

(2021) Dieu An H. Nguyen et al.   Nature Communications

Novel LOTUS-domain proteins are organizational hubs that recruit C. elegans Vasa to germ granules

(2021) Patricia Giselle Cipriani et al.   eLife

Protein-based condensation mechanisms drive the assembly of RNA-rich P granules

(2021) Helen Schmidt et al.   eLife

Small-RNA-mediated transgenerational silencing of histone genes impairs fertility in piRNA mutants

(2020) Giorgia Barucci et al.   NATURE CELL BIOLOGY

Recruitment of mRNAs to P granules by condensation with intrinsically-disordered proteins

(2020) Chih-Yung S Lee et al.   eLife

The Dynamics of P Granule Liquid Droplets Are Regulated by the Caenorhabditiselegans Germline RNA Helicase GLH-1 via Its ATP Hydrolysis Cycle

(2020) Wenjun Chen et al.   GENETICS

A Family of Argonaute-Interacting Proteins Gates Nuclear RNAi

(2020) Alexandra Lewis et al.   MOLECULAR CELL

poly(UG)-tailed RNAs in genome protection and epigenetic inheritance

(2020) Aditi Shukla et al.   NATURE

RNAi pathways repress reprogramming of C. elegans germ cells during heat stress

(2020) Alicia K Rogers et al.   NUCLEIC ACIDS RESEARCH

A tudor domain protein, SIMR-1, promotes siRNA production at piRNA-targeted mRNAs in C. elegans

(2020) Kevin I Manage et al.   eLife

Mutator Foci Are Regulated by Developmental Stage, RNA, and the Germline Cell Cycle in Caenorhabditis elegans

(2020) Celja J. Uebel et al.   G3-Genes Genomes Genetics

The nucleolus as a multiphase liquid condensate

(2020) Denis L. J. Lafontaine et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

DEPS-1 is required for piRNA-dependent silencing and PIWI condensate organisation in Caenorhabditis elegans

(2020) Kin Man Suen et al.   Nature Communications

A Small-RNA-Mediated Feedback Loop Maintains Proper Levels of 22G-RNAs in C. elegans

(2020) Alicia K. Rogers et al.   Cell Reports

Intrinsically disordered protein PID‐2 modulates Z granules and is required for heritable piRNA‐induced silencing in the Caenorhabditis elegans embryo

(2020) Maria Placentino et al.   EMBO JOURNAL

A gel phase promotes condensation of liquid P granules in Caenorhabditis elegans embryos

(2019) Andrea Putnam et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

MINA-1 and WAGO-4 are part of regulatory network coordinating germ cell death and RNAi in C. elegans

(2019) Ataman Sendoel et al.   CELL DEATH AND DIFFERENTIATION

Autophagy of germ-granule components, PGL-1 and PGL-3, contributes to DNA damage-induced germ cell apoptosis in C. elegans

(2019) Hyemin Min et al.   PLoS Genetics

PETISCO is a novel protein complex required for 21U RNA biogenesis and embryonic viability

(2019) Ricardo J. Cordeiro Rodrigues et al.   GENES & DEVELOPMENT

Functional Proteomics Identifies a PICS Complex Required for piRNA Maturation and Chromosome Segregation

(2019) Chenming Zeng et al.   Cell Reports

Germ Granules Govern Small RNA Inheritance

(2019) Itamar Lev et al.   CURRENT BIOLOGY

Germ Granules Coordinate RNA-Based Epigenetic Inheritance Pathways

(2019) Anne E. Dodson et al.   DEVELOPMENTAL CELL

P Granules Protect RNA Interference Genes from Silencing by piRNAs

(2019) John Paul T. Ouyang et al.   DEVELOPMENTAL CELL

Germline Maintenance Through the Multifaceted Activities of GLH/Vasa in Caenorhabditis elegans P Granules

(2019) Elisabeth A. Marnik et al.   GENETICS

Visualization and Quantification of Transposon Activity in Caenorhabditis elegans RNAi Pathway Mutants

(2019) Dylan C. Wallis et al.   G3-Genes Genomes Genetics

A PUF Hub Drives Self-Renewal in Caenorhabditis elegans Germline Stem Cells

(2019) Kimberly A. Haupt et al.   GENETICS

Widespread roles for piRNAs and WAGO-class siRNAs in shaping the germline transcriptome of Caenorhabditis elegans

(2019) Kailee J Reed et al.   NUCLEIC ACIDS RESEARCH

Identification of piRNA Binding Sites Reveals the Argonaute Regulatory Landscape of the C. elegans Germline

(2018) En-Zhi Shen et al.   CELL

ZNFX-1 Functions within Perinuclear Nuage to Balance Epigenetic Signals

(2018) Takao Ishidate et al.   MOLECULAR CELL

The piRNA targeting rules and the resistance to piRNA silencing in endogenous genes

(2018) Donglei Zhang et al.   SCIENCE

A Cytoplasmic Argonaute Protein Promotes the Inheritance of RNAi

(2018) Fei Xu et al.   Cell Reports

Binucleate germ cells in Caenorhabditis elegans are removed by physiological apoptosis

(2018) Stephan A. Raiders et al.   PLoS Genetics

mTOR Regulates Phase Separation of PGL Granules to Modulate Their Autophagic Degradation

(2018) Gangming Zhang et al.   CELL

Somatic and Germline MicroRNAs Form Distinct Silencing Complexes to Regulate Their Target mRNAs Differently

(2018) Alexandra Dallaire et al.   DEVELOPMENTAL CELL

The P Granules of C. elegans: A Genetic Model for the Study of RNA–Protein Condensates

(2018) Geraldine Seydoux   JOURNAL OF MOLECULAR BIOLOGY

Spatiotemporal regulation of liquid-like condensates in epigenetic inheritance

(2018) Gang Wan et al.   NATURE

Distinct regions of the intrinsically disordered protein MUT-16 mediate assembly of a small RNA amplification complex and promote phase separation of Mutator foci

(2018) Celja J. Uebel et al.   PLoS Genetics

The RNAi Inheritance Machinery of Caenorhabditis elegans

(2017) George Spracklin et al.   GENETICS

Germ Granules Prevent Accumulation of Somatic Transcripts in the AdultCaenorhabditis elegansGermline

(2017) Andrew Kekūpa’a Knutson et al.   GENETICS

ALG-5 is a miRNA-associated Argonaute required for proper developmental timing in the Caenorhabditis elegans germline

(2017) Kristen C. Brown et al.   NUCLEIC ACIDS RESEARCH

Convergent evolution of germ granule nucleators: A hypothesis

(2017) Arpita Kulkarni et al.   Stem Cell Research

ELLI-1, a novel germline protein, modulates RNAi activity and P-granule accumulation in Caenorhabditis elegans

(2017) Karolina M. Andralojc et al.   PLoS Genetics

The RNAi Inheritance Machinery of Caenorhabditis elegans

(2017) George Spracklin et al.   GENETICS

Germ Granules Prevent Accumulation of Somatic Transcripts in the AdultCaenorhabditis elegansGermline

(2017) Andrew Kekūpa’a Knutson et al.   GENETICS

The RNase PARN-1 Trims piRNA 3′ Ends to Promote Transcriptome Surveillance in C. elegans

(2016) Wen Tang et al.   CELL

Polar Positioning of Phase-Separated Liquid Compartments in Cells Regulated by an mRNA Competition Mechanism

(2016) Shambaditya Saha et al.   CELL

Coexisting Liquid Phases Underlie Nucleolar Subcompartments

(2016) Marina Feric et al.   CELL

In VivoInteraction Proteomics inCaenorhabditis elegansEmbryos Provides New Insights into P Granule Dynamics

(2016) Jia-Xuan Chen et al.   MOLECULAR & CELLULAR PROTEOMICS

PGL germ granule assembly protein is a base-specific, single-stranded RNase

(2016) Scott T. Aoki et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Transport Selectivity of Nuclear Pores, Phase Separation, and Membraneless Organelles

(2016) H. Broder Schmidt et al.   TRENDS IN BIOCHEMICAL SCIENCES

Somatically expressed germ-granule components, PGL-1 and PGL-3, repress programmed cell death in C. elegans

(2016) Mohammad Al-Amin et al.   Scientific Reports

Spatial patterning of P granules by RNA-induced phase separation of the intrinsically-disordered protein MEG-3

(2016) Jarrett Smith et al.   eLife

A Ribonuclease Coordinates siRNA Amplification and mRNA Cleavage during RNAi

(2015) Hsin-Yue Tsai et al.   CELL

CSR-1 and P granules suppress sperm-specific transcription in the C. elegans germline

(2015) A. C. Campbell et al.   DEVELOPMENT

piRNAs and piRNA-Dependent siRNAs Protect Conserved and Essential C. elegans Genes from Misrouting into the RNAi Pathway

(2015) Carolyn M. Phillips et al.   DEVELOPMENTAL CELL

Germ-Granule Components Prevent Somatic Development in the C. elegans Germline

(2014) Dustin L. Updike et al.   CURRENT BIOLOGY

The Vasa Homolog RDE-12 Engages Target mRNA and Multiple Argonaute Proteins to Promote RNAi in C. elegans

(2014) Masaki Shirayama et al.   CURRENT BIOLOGY

MUT-14 and SMUT-1 DEAD Box RNA Helicases Have Overlapping Roles in Germline RNAi and Endogenous siRNA Formation

(2014) Carolyn M. Phillips et al.   CURRENT BIOLOGY

The DEAD Box Helicase RDE-12 Promotes Amplification of RNAi in Cytoplasmic Foci in C. elegans

(2014) Huan Yang et al.   CURRENT BIOLOGY

Global effects of the CSR-1 RNA interference pathway on the transcriptional landscape

(2014) Germano Cecere et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Caenorhabditis elegans RSD-2 and RSD-6 promote germ cell immortality by maintaining small interfering RNA populations

(2014) A. Sakaguchi et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Reduced Insulin/IGF-1 Signaling Restores Germ Cell Immortality to Caenorhabditis elegans Piwi Mutants

(2014) Matt Simon et al.   Cell Reports

Regulation of RNA granule dynamics by phosphorylation of serine-rich, intrinsically disordered proteins in C. elegans

(2014) Jennifer T Wang et al.   eLife

Argonautes Promote Male Fertility and Provide a Paternal Memory of Germline Gene Expression in C. elegans

(2013) Colin C. Conine et al.   CELL

The C. elegans CSR-1 Argonaute Pathway Counteracts Epigenetic Silencing to Promote Germline Gene Expression

(2013) Meetu Seth et al.   DEVELOPMENTAL CELL

Protection of Germline Gene Expression by the C. elegans Argonaute CSR-1

(2013) Christopher J. Wedeles et al.   DEVELOPMENTAL CELL

C. elegans piRNAs Mediate the Genome-wide Surveillance of Germline Transcripts

(2012) Heng-Chi Lee et al.   CELL

CapSeq and CIP-TAP Identify Pol II Start Sites and Reveal Capped Small RNAs as C. elegans piRNA Precursors

(2012) Weifeng Gu et al.   CELL

piRNAs Can Trigger a Multigenerational Epigenetic Memory in the Germline of C. elegans

(2012) Alyson Ashe et al.   CELL

piRNAs Initiate an Epigenetic Memory of Nonself RNA in the C. elegans Germline

(2012) Masaki Shirayama et al.   CELL

The Caenorhabditis elegans RDE-10/RDE-11 Complex Regulates RNAi by Promoting Secondary siRNA Amplification

(2012) Chi Zhang et al.   CURRENT BIOLOGY

The P granule component PGL-1 promotes the localization and silencing activity of the PUF protein FBF-2 in germline stem cells

(2012) E. Voronina et al.   DEVELOPMENT

PAN-1, a P-granule component important for C. elegans fertility, has dual roles in the germline and soma

(2012) Ge Gao et al.   DEVELOPMENTAL BIOLOGY

Extremely stable Piwi-induced gene silencing inCaenorhabditis elegans

(2012) Maartje J Luteijn et al.   EMBO JOURNAL

MUT-16 promotes formation of perinuclear Mutator foci required for RNA silencing in the C. elegans germline

(2012) C. M. Phillips et al.   GENES & DEVELOPMENT

A nuclear Argonaute promotes multigenerational epigenetic inheritance and germline immortality

(2012) Bethany A. Buckley et al.   NATURE

Function, Targets, and Evolution of Caenorhabditis elegans piRNAs

(2012) M. P. Bagijn et al.   SCIENCE

The Caenorhabditis elegans HEN1 Ortholog, HENN-1, Methylates and Stabilizes Select Subclasses of Germline Small RNAs

(2012) Allison C. Billi et al.   PLoS Genetics

PIWI Associated siRNAs and piRNAs Specifically Require the Caenorhabditis elegans HEN1 Ortholog henn-1

(2012) Taiowa A. Montgomery et al.   PLoS Genetics

Differential Impact of the HEN1 Homolog HENN-1 on 21U and 26G RNAs in the Germline of Caenorhabditis elegans

(2012) Leonie M. Kamminga et al.   PLoS Genetics

P granules extend the nuclear pore complex environment in theC. elegansgerm line

(2011) Dustin L. Updike et al.   JOURNAL OF CELL BIOLOGY

PGL proteins self associate and bind RNPs to mediate germ granule assembly inC. elegans

(2011) Momoyo Hanazawa et al.   JOURNAL OF CELL BIOLOGY

Tudor domain ERI-5 tethers an RNA-dependent RNA polymerase to DCR-1 to potentiate endo-RNAi

(2011) Caroline Thivierge et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

mut-16 and other mutator class genes modulate 22G and 26G siRNA pathways in Caenorhabditis elegans

(2011) C. Zhang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The C. elegans homolog of nucleoporin Nup98 is required for the integrity and function of germline P granules

(2010) E. Voronina et al.   DEVELOPMENT

Perinuclear P granules are the principal sites of mRNA export in adult C. elegans germ cells

(2010) U. Sheth et al.   DEVELOPMENT

C. elegans Dicer interacts with the P-granule component GLH-1 and both regulate germline RNPs

(2010) Erica L. Beshore et al.   DEVELOPMENTAL BIOLOGY

P Granule Assembly and Function in Caenorhabditis elegans Germ Cells

(2010) D. Updike et al.   JOURNAL OF ANDROLOGY

Distinct Phases of siRNA Synthesis in an Endogenous RNAi Pathway in C. elegans Soma

(2010) Jonathan I. Gent et al.   MOLECULAR CELL

Argonautes ALG-3 and ALG-4 are required for spermatogenesis-specific 26G-RNAs and thermotolerant sperm in Caenorhabditis elegans

(2010) Colin C. Conine et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

SEPA-1 Mediates the Specific Recognition and Degradation of P Granule Components by Autophagy in C. elegans

(2009) Yuxia Zhang et al.   CELL

The Argonaute CSR-1 and Its 22G-RNA Cofactors Are Required for Holocentric Chromosome Segregation

(2009) Julie M. Claycomb et al.   CELL

CDE-1 Affects Chromosome Segregation through Uridylation of CSR-1-Bound siRNAs

(2009) Josien C. van Wolfswinkel et al.   CELL

The C. elegans sex determination gene laf-1 encodes a putative DEAD-box RNA helicase

(2009) Amy Hubert et al.   DEVELOPMENTAL BIOLOGY

Antagonism between GLD-2 Binding Partners Controls Gamete Sex

(2009) Kyung Won Kim et al.   DEVELOPMENTAL CELL

Two conserved regulatory cytoplasmic poly(A) polymerases, GLD-4 and GLD-2, regulate meiotic progression in C. elegans

(2009) M. Schmid et al.   GENES & DEVELOPMENT

A Genomewide RNAi Screen for Genes That Affect the Stability, Distribution and Function of P Granules in Caenorhabditis elegans

(2009) Dustin L. Updike et al.   GENETICS

An eIF4E-binding protein regulates katanin protein levels inC. elegansembryos

(2009) Wei Li et al.   JOURNAL OF CELL BIOLOGY

A germline-specific isoform of eIF4E (IFE-1) is required for efficient translation of stored mRNAs and maturation of both oocytes and sperm

(2009) M. A. Henderson et al.   JOURNAL OF CELL SCIENCE

Distinct Argonaute-Mediated 22G-RNA Pathways Direct Genome Surveillance in the C. elegans Germline

(2009) Weifeng Gu et al.   MOLECULAR CELL

Germline P Granules Are Liquid Droplets That Localize by Controlled Dissolution/Condensation

(2009) C. P. Brangwynne et al.   SCIENCE

GLS-1, a Novel P Granule Component, Modulates a Network of Conserved RNA Regulators to Influence Germ Cell Fate Decisions

(2009) Agata Rybarska et al.   PLoS Genetics

A C. elegans Piwi, PRG-1, Regulates 21U-RNAs during Spermatogenesis

(2008) Guilin Wang et al.   CURRENT BIOLOGY

DEPS-1 promotes P-granule assembly and RNA interference in C. elegans germ cells

(2008) C. A. Spike et al.   DEVELOPMENT

Processing bodies and germ granules are distinct RNA granules that interact in C. elegans embryos

(2008) Christopher M. Gallo et al.   DEVELOPMENTAL BIOLOGY

C. elegans pur alpha, an activator of end-1, synergizes with the Wnt pathway to specify endoderm

(2008) Eric S. Witze et al.   DEVELOPMENTAL BIOLOGY

C. elegans RNA-binding proteins PUF-8 and MEX-3 function redundantly to promote germline stem cell mitosis

(2008) Mohd Ariz et al.   DEVELOPMENTAL BIOLOGY

Genetic Analysis of the Caenorhabditis elegans GLH Family of P-Granule Proteins

(2008) C. Spike et al.   GENETICS

MEG-1 and MEG-2 Are Embryo-Specific P-Granule Components Required for Germline Development in Caenorhabditis elegans

(2008) Stefanie W. Leacock et al.   GENETICS

The Caenorhabditis elegans rsd-2 and rsd-6 Genes Are Required for Chromosome Functions During Exposure to Unfavorable Environments

(2008) W. Han et al.   GENETICS

PRG-1 and 21U-RNAs Interact to Form the piRNA Complex Required for Fertility in C. elegans

(2008) Pedro J. Batista et al.   MOLECULAR CELL