Synthetic reconstruction of the hunchback promoter specifies the role of Bicoid, Zelda and Hunchback in the dynamics of its transcription

A mechanism for hunchback promoters to readout morphogenetic positional information in less than a minute

(2020) Jonathan Desponds et al.   eLife

Dense and pleiotropic regulatory information in a developmental enhancer

(2020) Timothy Fuqua et al.   NATURE

Quantitative dissection of transcription in development yields evidence for transcription factor-driven chromatin accessibility

(2020) Elizabeth Eck et al.   eLife

Continued Activity of the Pioneer Factor Zelda Is Required to Drive Zygotic Genome Activation

(2019) Stephen L. McDaniel et al.   MOLECULAR CELL

Dissecting the sharp response of a canonical developmental enhancer reveals multiple sources of cooperativity

(2019) Jeehae Park et al.   eLife

Bicoid-Dependent Activation of the Target Gene hunchback Requires a Two-Motif Sequence Code in a Specific Basal Promoter

(2019) Jia Ling et al.   MOLECULAR CELL

Multimodal transcriptional control of pattern formation in embryonic development

(2019) Nicholas C. Lammers et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Mitotic waves in the early embryogenesis ofDrosophila: Bistability traded for speed

(2018) Massimo Vergassola et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Bicoid gradient formation mechanism and dynamics revealed by protein lifetime analysis

(2018) Lucia Durrieu et al.   Molecular Systems Biology

Temporal control of gene expression by the pioneer factor Zelda through transient interactions in hubs

(2018) Jeremy Dufourt et al.   Nature Communications

Precision in a rush: Trade-offs between reproducibility and steepness of the hunchback expression pattern

(2018) Huy Tran et al.   PLoS Computational Biology

3 minutes to precisely measure morphogen concentration

(2018) Tanguy Lucas et al.   PLoS Genetics

Dynamic multifactor hubs interact transiently with sites of active transcription in Drosophila embryos

(2018) Mustafa Mir et al.   eLife

Dense Bicoid hubs accentuate binding along the morphogen gradient

(2017) Mustafa Mir et al.   GENES & DEVELOPMENT

The Hunchback temporal transcription factor establishes, but is not required to maintain, early-born neuronal identity

(2017) Keiko Hirono et al.   Neural Development

Decoding temporal interpretation of the morphogen Bicoid in the early Drosophila embryo

(2017) Anqi Huang et al.   eLife

Concentration dependent chromatin states induced by the bicoid morphogen gradient

(2017) Colleen E Hannon et al.   eLife

Information Integration and Energy Expenditure in Gene Regulation

(2016) Javier Estrada et al.   CELL

The appeasement of Doug: a synthetic approach to enhancer biology

(2016) Ben J. Vincent et al.   Integrative Biology

Syntax compensates for poor binding sites to encode tissue specificity of developmental enhancers

(2016) Emma K. Farley et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Precision of Readout at the hunchback Gene: Analyzing Short Transcription Time Traces in Living Fly Embryos

(2016) Jonathan Desponds et al.   PLoS Computational Biology

New methods to image transcription in living fly embryos: the insights so far, and the prospects

(2016) Teresa Ferraro et al.   Wiley Interdisciplinary Reviews-Developmental Biology

Combining protein and mRNA quantification to decipher transcriptional regulation

(2015) Heng Xu et al.   NATURE METHODS

Maternal Origins of Developmental Reproducibility

(2014) Mariela D. Petkova et al.   CURRENT BIOLOGY

Zelda Potentiates Morphogen Activity by Increasing Chromatin Accessibility

(2014) Sun Melody Foo et al.   CURRENT BIOLOGY

Impacts of the ubiquitous factor Zelda on Bicoid-dependent DNA binding and transcription in Drosophila

(2014) Z. Xu et al.   GENES & DEVELOPMENT

Dynamic regulation of eve stripe 2 expression reveals transcriptional bursts in living Drosophila embryos

(2014) J. P. Bothma et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Optimized CRISPR/Cas tools for efficient germline and somatic genome engineering in Drosophila

(2014) F. Port et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Subtle Changes in Motif Positioning Cause Tissue-Specific Effects on Robustness of an Enhancer's Activity

(2014) Jelena Erceg et al.   PLoS Genetics

Precise Developmental Gene Expression Arises from Globally Stochastic Transcriptional Activity

(2013) Shawn C. Little et al.   CELL

Live Imaging of Bicoid-Dependent Transcription in Drosophila Embryos

(2013) Tanguy Lucas et al.   CURRENT BIOLOGY

Quantitative Imaging of Transcription in Living Drosophila Embryos Links Polymerase Activity to Patterning

(2013) Hernan G. Garcia et al.   CURRENT BIOLOGY

Dynamic interpretation of maternal inputs by the Drosophila segmentation gene network

(2013) F. Liu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Systematic dissection and optimization of inducible enhancers in human cells using a massively parallel reporter assay

(2012) Alexandre Melnikov et al.   NATURE BIOTECHNOLOGY

Inferring gene regulatory logic from high-throughput measurements of thousands of systematically designed promoters

(2012) Eilon Sharon et al.   NATURE BIOTECHNOLOGY

Massively parallel functional dissection of mammalian enhancers in vivo

(2012) Rupali P Patwardhan et al.   NATURE BIOTECHNOLOGY

Zelda Binding in the Early Drosophila melanogaster Embryo Marks Regions Subsequently Activated at the Maternal-to-Zygotic Transition

(2011) Melissa M. Harrison et al.   PLoS Genetics

High Mobility of Bicoid Captured by Fluorescence Correlation Spectroscopy: Implication for the Rapid Establishment of Its Gradient

(2010) Asmahan Abu-Arish et al.   BIOPHYSICAL JOURNAL

The time to measure positional information: maternal Hunchback is required for the synchrony of the Bicoid transcriptional response at the onset of zygotic transcription

(2010) A. Porcher et al.   DEVELOPMENT

Predicting expression patterns from regulatory sequence in Drosophila segmentation

(2008) Eran Segal et al.   NATURE

The zinc-finger protein Zelda is a key activator of the early zygotic genome in Drosophila

(2008) Hsiao-Lan Liang et al.   NATURE

Analysis of combinatorial cis-regulation in synthetic and genomic promoters

(2008) Jason Gertz et al.   NATURE