Extremophiles and extremozymes in lignin bioprocessing

Lignin is the most abundant aromatic biopolymer resource in nature. Due to its complexity and highly polymerized aromatic structure, lignin is strenuous to be merely degraded by normal microorganisms. Lignin degrading microbes from extreme environments are considered suitable candidates for lignin bioprocessing. The heterogeneity of the depolymerized products hinders its biorefinery and high-value utilization of its potential products. The complexity and diversity of lignin biodegradation pathways require an arsenal of versatile lignin-degrading enzymes, including lignin peroxidase, laccase, manganese peroxidase, dye decolorizing enzyme, dehydrogenase, superoxide dismutase, cytochrome oxidase, monooxygenase, dioxygenase, O-demethylase, and methyltransferase. Several of these extremozymes were isolated from extremophiles, which are characterized by their activity under extreme conditions. The advantages of extremophiles and extremozymes rely on their capacity to withstand harsh environmental conditions, thus display superior performance, under favorable conditions in biomass pretreatment, lignin depolymerization, biotransformation and chemical production processes. Therefore, extremophiles and extremozymes are indeed very promising for efficient degradation and utilization of lignin. However, due to the particular physiological characteristics of extremophiles and their enzymes and the lack of tailored molecular biological tools, it is more challenging to study these microorganisms than ordinary microorganisms. Integrated transcriptomics, proteomics and metabolomics analysis of ligninolytic extremophiles is expected to unleash functional aspects of lignin biotransformation. In addition, heterologous expression of genes and operons from extremophiles in industrially relevant bacterial and fungal strains are required.

Automated summary

Lignin is a common and abundant aromatic biopolymer in nature. Due to its complexity and highly polymerized structure, normal microorganisms struggle to degrade lignin. Microbes from extreme environments that can degrade lignin are considered promising candidates for lignin bioprocessing. The heterogeneity of lignin depolymerization products poses challenges for refining and utilizing its potential. Various versatile lignin-degrading enzymes, including lignin peroxidase, laccase, manganese peroxidase, and others, are required for the complex lignin degradation pathways. Extremozymes isolated from extremophiles have shown superior performance in harsh conditions, making them promising for efficient degradation and utilization of lignin. However, studying extremophiles and their enzymes is more challenging due to their unique physiological characteristics and the lack of tailored molecular tools. Integrated transcriptomics, proteomics, and metabolomics analysis are expected to provide insights into lignin biotransformation. Furthermore, heterologous expression of extremophile genes in industrially relevant strains is necessary.