Article
Biochemistry & Molecular Biology
Sarah Vanwijnsberghe, Charlotte Peeters, Emmelie De Ridder, Charles Dumolin, Anneleen D. Wieme, Nico Boon, Peter Vandamme
Summary: The study found multiple Paraburkholderia isolates and species in pristine forest soil, capable of degrading aromatic compounds, possibly due to the abundant presence of aromatic compounds in decomposing plant litter, reflecting microbial habitat diversity or synergistic relationships.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Microbiology
Lea Girard, Cedric Lood, Rene De Mot, Vera van Noort, Julia Baudart
Summary: Recent taxonomy changes have led to the delineation of three new genera within the Pseudomonadaceae family. Pseudomonas, however, remains the most diverse genus and can produce a variety of secondary metabolites that play important ecological roles. This study focuses on the genetic diversity and metabolic potential of marine Pseudomonas strains, revealing incorrect species assignments and the presence of new species.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Mingliang Zhang, Yijun Ren, Wankui Jiang, Chenglong Wu, Yidong Zhou, Hui Wang, Zhijian Ke, Qinqin Gao, Xiaoan Liu, Jiguo Qiu, Qing Hong
Summary: The study identified two Paenarthrobacter strains capable of degrading the fungicide iprodione through a conserved metabolic pathway. Comparative genomic analysis revealed the presence of iprodione catabolic genes on plasmids in both strains, showing high conservation between them. This enhances the understanding of microbial degradation mechanisms of iprodione.
ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Daniela Chmelova, Barbora Legerska, Miroslav Ondrejovic, Stanislav Miertus
Summary: The study aimed to optimize a synthetic medium for maximum biomass yield using RSM, achieving a biomass yield of 1.71 g/L due to the optimal carbon and nitrogen sources in the medium. This approach demonstrates the potential of using two-stage PHA cultivation to obtain the maximum amount of biomass and PHA.
FERMENTATION-BASEL
(2022)
Article
Environmental Sciences
Mengyuan Qi, Xiaodan Ma, Bin Liang, Liying Zhang, Deyong Kong, Zhiling Li, Aijie Wang
Summary: The study focused on two important degraders of sulfonamide antibiotics, investigating their complete genomes and verifying complete degradation and bacterial growth. Results showed that one of the strains, P27, not only degraded sulfonamide antibiotics but also transformed them into nitrogen-containing heterocyclic products, indicating a broad substrate spectrum and significant bioremediation potential.
ENVIRONMENTAL RESEARCH
(2022)
Article
Polymer Science
Linxin Song, Ming Wang, Dengbin Yu, Yu Li, Hongwen Yu, Xuerong Han
Summary: This study successfully improved the production of medium-chain-length polyhydroxyalkanoates (mcl-PHAs) by genetic engineering methods. Knocking out the phaZ gene and inserting a tac enhancer into the phaC1/phaC2 genes were effective ways to enhance mcl-PHA yields. The synthesized products were similar to those synthesized by the wild-type strain. GPC analysis showed a decrease in the relative molecular weight of depolymerized polyester compared to the wild-type strain. DSC analysis revealed lower melting points for mcl-PHAs produced by recombinant strains. Finally, TG analysis indicated higher decomposition temperatures for mcl-PHAs synthesized by the recombinant strains compared to the wild-type strain.
Article
Biotechnology & Applied Microbiology
Xiao Fu, Qing Gong, Xuan Liu, Ze Zheng, Xiaoyu Zhang, Fuying Ma, Hongbo Yu, Shangxian Xie
Summary: Producing polyhydroxyalkanoate (PHA) from lignin through a Fenton-like reaction reported in this study significantly improved the bioconversion of lignin to PHA by 141.7% compared to Kraft lignin. The bond cleavage of lignin was characterized, and low toxic mono-aromatic and low molecular organic compounds were produced for microbial conversion. This novel approach mimicking fungal decay strategy coupled with the microbial conversion of lignin into high-value PHA holds great potential for a sustainable future.
FERMENTATION-BASEL
(2023)
Article
Polymer Science
Beom-Jung Kang, Jong-Min Jeon, Shashi Kant Bhatia, Do-Hyung Kim, Yung-Hun Yang, Sangwon Jung, Jeong-Jun Yoon
Summary: Coffee waste can be used to extract oil and produce polyhydroxyalkanoate (PHA). The extracted oil can also be used for biohydrogen production. The oil extraction yield was 14.4% and accounted for 97% of the oil in the waste. The extracted waste had a higher sugar concentration and resulted in more hydrogen production. Additionally, using the extracted oil for culture resulted in higher cell dry weight, PHA production, and PHA content.
Article
Agricultural Engineering
Miho Akutsu, Nanase Abe, Chiho Sakamoto, Yuki Kurimoto, Haruka Sugita, Makoto Tanaka, Yudai Higuchi, Kimitoshi Sakamoto, Naofumi Kamimura, Hiroyuki Kurihara, Eiji Masai, Tomonori Sonoki
Summary: The Pseudomonas sp. NGC7 strain has been shown to produce glucose-free ccMA even from a mixture of lignin-derived aromatics that does not contain essential syringyl nucleus compounds. By introducing a gene set for the PCA-shunt and making certain genetic modifications, a ccMA-producing strain that grows on a lignin-derived aromatics mixture was successfully constructed. The engineered strain demonstrated high ccMA production efficiency from sugar cane bagasse alkaline extract.
BIORESOURCE TECHNOLOGY
(2022)
Article
Microbiology
Muhammed Duman, Magdalena Mulet, Soner Altun, Izzet Burcin Saticioglu, Margarita Gomila, Jorge Lalucat, Elena Garcia-Valdes
Summary: Six Gram-negative, motile bacteria were isolated from rainbow trout, and were identified as belonging to four novel species in the genus Pseudomonas through DNA analysis and biochemical tests.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Joao R. Pereira, Diana Araujo, Patricia Freitas, Ana C. Marques, Vitor D. Alves, Chantal Sevrin, Christian Grandfils, Elvira Fortunato, Maria A. M. Reis, Filomena Freitas
Summary: Pseudomonas chlororaphis subsp. aurantiaca DSM 19603 was cultivated on apple pulp to produce medium-chain length polyhydroxyalkanoates with attractive filming properties, showing potential applications in various industries such as fine chemicals, biomedicine, pharmaceuticals, and food packaging.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Microbiology
Julia B. Gontijo, Fabiana S. Paula, Andressa M. Venturini, Jessica A. Mandro, Paul L. E. Bodelier, Siu M. Tsai
Summary: This study described the metagenome-assembled genome (MAG) of Methylocystis sp. from Amazonian floodplains sediments and explored its functional potential and ecological traits. The analysis revealed that it belongs to Methylocystis parvus and has the ability for methane oxidation and nitrogen fixation, as well as unique genes related to nitrogen metabolism and cell motility.
Article
Multidisciplinary Sciences
Mouna Mahjoubi, Habibu Aliyu, Mohamed Neifar, Simone Cappello, Habib Chouchane, Yasmine Souissi, Ahmed Salaheddine Masmoudi, Don A. Cowan, Ameur Cherif
Summary: The study demonstrates that the Pseudomonas BUN14 strain has a versatile hydrocarbon degradation capacity, making it a promising candidate for bioremediation in polluted saline environments.
SCIENTIFIC REPORTS
(2021)
Article
Fisheries
Jose M. Vidal, Alexis Fonseca, Pamela Ruiz, Daniela Sepulveda, Carlos Carrasco, Stefano Scilipoti, Javier Barros, Ariel Valenzuela, Ricardo Saavedra, Nathaly Ruiz-Tagle, Homero Urrutia
Summary: This study evaluated the probiotic potential of the biofilm formed by Pseudomonas sp. RGM2144 strain on rainbow trout survival. The results showed that Pseudomonas sp. RGM2144 significantly increased rainbow trout survival and exhibited characteristics of a new species with few virulence factors, suggesting its potential as a non-pathogenic bacterium with protective effects.
JOURNAL OF FISH DISEASES
(2023)
Article
Agricultural Engineering
Estel Rueda, Beatriz Altamira-Algarra, Joan Garcia
Summary: The effect of four parameters on PHB production in Synechococcus sp. and Synechocystis sp. was tested and optimized. Different optimal conditions were found for the two strains.
BIORESOURCE TECHNOLOGY
(2022)
Article
Chemistry, Analytical
Xiao-Meng Ma, Jian-Wei Wang, Li-Ting Zhao, Yafei Zhang, Jun-Ying Liu, Songmei Wang, Daochen Zhu, Zhugen Yang, Yang-Chun Yong
Summary: A novel microfiber-like biohydrogel was developed using electroactive bacteria-induced graphene oxide reduction and confined self-assembly in a capillary tube. This biohydrogel enabled efficient electron exchange between cells and the conductive graphene network, and was used to create a miniature whole-cell electrochemical biosensing system for fumarate detection. The system exhibited high sensitivity and a low limit of detection, offering new possibilities for bioelectrochemical detection in small volumes or at high spatial resolutions.
ANALYTICAL CHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Huayou Chen, Xiaoyu Liang, Shouzhi Li, Zhoulei Cui, Yangchun Yong, Zhong Ni, Quan Bu, Daochen Zhu
Summary: By studying the effects of small molecules on the efficiency of lignin degradation by lignin peroxidase, the utility value of enzymes was maximized. Using wheat straw as raw material, it was found that laccase, glucose oxidase, malonic acid, citric acid, ZnSO4, CaCl2 could promote the lignin degradation by the lignin peroxidase. Moreover, glucose oxidase, malonic acid and CaCl2 had obvious synergy effects on lignin degradation by the lignin peroxidase.
BIOTECHNOLOGY LETTERS
(2023)
Article
Engineering, Environmental
Sameh Samir Ali, Tamer Elsamahy, Daochen Zhu, Jianzhong Sun
Summary: This study investigated the potential of gut symbionts of lesser waxworm larvae to degrade low-density polyethylene (LDPE). Two bacterial isolates, identified as Citrobacter freundii and Bacillus sp., showed the highest reduction in LDPE's tensile strength. The cell density of these bacteria increased over time, indicating their high activity in degrading LDPE. These findings provide promising evidence for the biodegradation of plastic waste management in the environment.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Review
Environmental Sciences
Ashenafi Berhanu, Ishmael Mutanda, Ji Taolin, Majjid A. Qaria, Bin Yang, Daochen Zhu
Summary: Since the 1950s, large amounts of PFAS have been dumped into the environment, causing significant contamination. PFAS are resistant to degradation and have adverse effects on health and the environment. Traditional physicochemical methods for PFAS treatment have drawbacks, making microbial degradation and enzymatic treatment highly sought after. This review provides an overview of PFAS classification, properties, and interaction with the environment, and discusses recent developments in microbial degradation, transformation routes, and challenges in bioremediation approaches.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Genetics & Heredity
Nisar Uddin, Noor Muhammad, Sameh Samir Ali, Riaz Ullah, Ahmed Bari, Hidayat Hussain, Daochen Zhu
Summary: Understanding the impacts and constraints of climate change on the geographical distribution of Ziziphus nummularia is crucial for its sustainability. This study analyzed field investigation and climate change data using ArcGIS analysis. Genetic diversity of 180 genotypes from three populations was studied using morphological attributes and simple sequence repeat (SSR). Results showed that mean temperature was a significant factor limiting the distribution. Current and future suitable regions were identified as Swat and Buner, respectively. Phenotypic traits and genetic diversity were assessed, indicating the presence of variability and unique alleles. Conservation strategies are required due to the impact of climate change on species distributions.
Article
Biochemical Research Methods
Mahammed Ilyas Khazi, Fakhra Liaqat, Wenhui Gu, Badr Mohamed, Daochen Zhu, Jian Li
Summary: This study investigates the development of a dual-substrate mixotrophy strategy to cultivate the microalga Haematococcus lacustris for astaxanthin production. The results showed that the dual-substrate mixotrophy significantly increased biomass productivity by up to 2-fold in the green growth phase compared to phototrophic controls. Furthermore, the supplementation of dual-substrate to the red phase increased astaxanthin accumulation by 10% compared to single-substrate acetate and no substrate. This approach shows promise for commercial production of biological astaxanthin in indoor closed systems.
BIOTECHNOLOGY JOURNAL
(2023)
Article
Environmental Sciences
Xu Chunyan, Majjid A. Qaria, Xu Qi, Zhu Daochen
Summary: Petroleum hydrocarbon compounds are persistent organic pollutants that can cause permanent damage to ecosystems due to their biomagnification. Bioremediation is currently the main solution for remediation of petroleum hydrocarbon pollutants in ecosystems. However, there are various challenges for microorganisms to perform efficiently in outside environments, making it necessary to explore efficient biodegradation technologies through the discovery of new microorganisms, modification of biodegradation pathways, and development of new bioremediation technologies.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Environmental Sciences
Md Muzammel Hossain, Iffat Jahan, Abdullah Al Nahian, Zhipeng Zhuang, Stephen J. Maxwell, Mohamed Yassin Ali, Sivasamy Sethupathy, Daochen Zhu
Summary: The consumption of contaminated finfish from the polluted river of Turag-Tongi-Balu, Kamarpara site, Dhaka poses significant health hazards to humans, with the concentrations of Cd, Mn, Pb, and Se exceeding the Food Safety Guideline value in most fish species. The high Mn concentrations in certain fish species elevate the non-carcinogenic risk, and there are statistically significant differences in heavy metal concentrations among fish species. The regular dietary consumption of fish from this polluted ecosystem poses a significant health risk and is indicated as cancer, emphasizing the importance of monitoring heavy metal contamination and minimizing the risk to human health.
ENVIRONMENTAL TOXICOLOGY
(2023)
Article
Agricultural Engineering
Zahoor, Meysam Madadi, Mudasir Nazar, Syed Waqas Ali Shah, Nana Li, Muhammad Imtiaz, Zijing Zhong, Daochen Zhu
Summary: A response surface design was used to pretreat sugarcane bagasse (SB) with urea (U) and potassium hydroxide (K) at low temperatures. The optimized pretreatment condition (-20 degrees C, 7.5% UK) resulted in increased removal of hemicellulose and lignin, as well as an increase in cellulose content compared to the control. The UK pretreatment also improved enzymatic hydrolysis and exhibited potential as a pretreatment technology for lignocellulosic valorization.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Microbiology
Ishmael Mutanda, Zahoor, Sivasamy Sethupathy, Qi Xu, Bin Zhu, Sayed Waqas Ali Shah, Zhipeng Zhuang, Daochen Zhu
Summary: This study optimized the production of bacterial laccase, a promising enzyme for lignin valorization and biomass processing, by improving culture medium composition and fermentation parameters. The optimized medium achieved a 3.3-fold yield improvement, and further optimization of fermentation parameters resulted in a 7-fold yield increase compared to initial conditions. This research provides a high-yielding and cost-efficient production system for bacterial laccase and has implications in various applications.
MICROBIOLOGICAL RESEARCH
(2023)
Review
Engineering, Manufacturing
Syed Waqas Ali Shah, Qi Xu, Muhammad Wajid Ullah, Sivasamy Sethupathy, Gabriel Murillo Morales, Jianzhong Sun, Daochen Zhu
Summary: Additive manufacturing, particularly using renewable natural polymers and synthetic materials, shows great potential in the manufacturing, pharmaceutical, food, and biomedical industries. Among these materials, lignin, which is a renewable bio-based raw material, is receiving significant attention due to its amorphous macromolecular structure and beneficial properties. However, the complex polymeric and amorphous structure of lignin presents challenges for its utilization in 3D printing. This review summarizes the use of lignin in the preparation of ink for 3D printing and discusses its merits, limitations, and future perspectives.
ADDITIVE MANUFACTURING
(2023)
Article
Environmental Sciences
Majjid A. Qaria, Chunyan Xu, Arif Hussain, Muhammad Zohaib Nawaz, Daochen Zhu
Summary: This study reveals the impact of oil pollution on soil microbial communities, with increased presence of bacterial species and higher expression of resistance and virulence factors.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Microbiology
Asad Ullah Khan, Muhammad Zahoor, Mujaddad Ur Rehman, Muhammad Ikram, Daochen Zhu, Muhammad Naveed Umar, Riaz Ullah, Essam A. Ali
Summary: Dye-polluted wastewater is a serious threat to human, animal, and plant health. To mitigate this, the treatment of wastewater containing dyes is necessary. This study demonstrates the use of Pseudomonas aeruginosa to degrade the textile azo dye brown 703. Optimization experiments showed that under specific conditions, the bacterial strain can effectively decolorize and degrade the dye within 3 days.
MICROBIOLOGY RESEARCH
(2023)