Article
Chemistry, Analytical
Hessamaddin Sohrabi, Mir Reza Majidi, Mina Fakhraei, Ali Jahanban-Esfahlan, Maryam Hejazi, Fatemeh Oroojalian, Behzad Baradaran, Maryam Tohidast, Miguel de la Guardia, Ahad Mokhtarzadeh
Summary: Point-of-care testing (POCT) analysis and bedside research have globally improved up-to-date diagnostics. Lateral flow assays (LFAs) have gained considerable attention due to their cost-effectiveness, portability, user-friendliness, and rapid detection. LFAs based on qualitative and optical outcomes have enhanced the objectivity and data efficiency of assessments. Therefore, developing novel methods to provide reliable and quantitative information while preserving the qualities of LFAs is of great necessity.
Review
Agriculture, Multidisciplinary
Yongxiang Ji, Yan Huang, Zhihao Cheng, Wenbin Hao, Guodong Liu, Yang Liu, Xueji Zhang
Summary: Rapid and sensitive detection of foodborne pathogenic bacteria is crucial for preventing and controlling foodborne diseases. The lateral flow strip biosensor (LFSB) is a promising point-of-care detection tool that is widely used in food safety monitoring. This review presents recent advances in the detection of foodborne pathogenic bacteria using LFSBs, summarizing the direct and indirect sensing strategies based on different bacterial biomarkers. The applications of direct and indirect sensing strategies are compared and discussed, and the existing challenges, future perspectives, and development directions of LFSBs are examined to promote theoretical innovation and practical application.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biophysics
Xiaojing Liu, Deepak Kukkar, Ziai Deng, Di Yang, Jianlong Wang, Ki-Hyun Kim, Daohong Zhang
Summary: This article provides a comprehensive review of lock-and-key recognizer-encoded lateral flow assays (LFAs) for the detection of foodborne pathogenic bacteria. The strategies for bacteria recognition, including antibody-based antigen-antibody interactions, nucleic acid aptamer-based recognition, and phage-mediated targeting of bacterial cells, are described. The article also highlights the technological challenges and future prospects for the development of LFAs in food analysis, emphasizing the need for high-quality bio-probes, multiplex sensors, and intelligent portable readers. LFAs based on various recognition strategies show great potential for rapid, convenient, and effective point-of-care detection of pathogens in complex food matrixes.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Biophysics
Towfeeq Fairooz, Sara E. McNamee, Dewar Finlay, Kok Yew Ng, James McLaughlin
Summary: Cardiovascular Disease (CVD) is a major cause of death globally, and there is a need for rapid detection and treatment. This paper presents a novel approach for generating and analyzing images from lateral flow assays (LFAs) to improve disease diagnosis. The proposed method utilizes artificial intelligence and image processing techniques to classify LFA images and provide accurate risk stratification levels.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Multidisciplinary
Sarah H. Klass, Laura E. Sofen, Zachary F. Hallberg, Tahoe A. Fiala, Alexandra Ramsey, Nicholas S. Dolan, Matthew B. Francis, Ariel L. Furst
Summary: Pathogenic E. coli strains are a significant threat to public health, causing both foodborne illnesses and urinary tract infections. By using a rapid bioconjugation reaction and electrochemical impedance spectroscopy, we can selectively capture and accurately quantify these pathogenic microbes on a disposable gold electrode.
CHEMICAL COMMUNICATIONS
(2021)
Article
Chemistry, Multidisciplinary
Haotian Wu, Yuan Fang, Li Tian, Xin Liu, Xiao Zhou, Xiying Chen, Heqi Gao, Haijuan Qin, Yaqing Liu
Summary: This article introduces a chemiluminescence-based ratiometric sensing platform for rapid detection of pathogenic bacteria. The platform can achieve accurate quantification of bacteria in real samples without the need for external excitation sources and hydrogen peroxide. It offers high sensitivity and simplicity in operation, making it a valuable tool for point-of-care testing.
Review
Microbiology
Dipanjan Dutta, Anupam Kaushik, Dhirendra Kumar, Satyabrata Bag
Summary: Foodborne illness caused by pathogenic Vibrios is often linked to raw or undercooked seafood consumption. Symptoms include watery diarrhea, stomach cramping, nausea, vomiting, fever, and chills. Treatment typically involves oral or intravenous rehydration salts and antibiotics.
FRONTIERS IN MICROBIOLOGY
(2021)
Review
Biochemical Research Methods
Jingbin Zhang, Yixiang Wang, Xiaonan Lu
Summary: Foodborne diseases caused by bacterial pathogens are a growing threat to public health worldwide. Rapid detection of pathogenic bacteria is crucial for disease prevention and food safety. Biosensors integrating molecular imprinted polymers and sensor platforms are promising candidates for highly sensitive and selective detection of pathogenic bacteria.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Review
Biochemical Research Methods
Fang Mi, Cunming Hu, Ying Wang, Li Wang, Fei Peng, PengFei Geng, Ming Guan
Summary: This article reviews the recent development of bio-recognition elements integrated on microfluidic chips and pathogen pretreatment in microfluidic chip technology. Furthermore, it summarizes and discusses the research progress of microfluidic technology based on optical and electrochemical sensors for the detection of foodborne pathogenic bacteria. Finally, the future prospects and challenges of microfluidic chips based on biosensors are presented.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Review
Food Science & Technology
Haotian Yu, Wei Guo, Xin Lu, Hui Xu, Qian Yang, Jianxin Tan, Wei Zhang
Summary: This review tracks the development of reduced graphene oxide (rGO) nanocomposite materials electrochemical biosensors for the detection of foodborne pathogenic bacteria, discussing the preparation of rGO nanocomposite electrochemical biosensors and their working mechanism.
Article
Food Science & Technology
Pengzhen Li, Xiaoxuan Feng, Baiyan Chen, Xiaoying Wang, Zuyue Liang, Li Wang
Summary: A multiplex PCR system was developed for simultaneous detection of eight foodborne pathogenic bacteria. The system exhibited high sensitivity and specificity, and showed good stability and sensitivity in artificially contaminated samples.
Article
Chemistry, Applied
Shuai Zheng, Xingsheng Yang, Bo Zhang, Siyun Cheng, Han Han, Qing Jin, Chongwen Wang, Rui Xiao
Summary: A silica-quantum dot-based fluorescent lateral flow immunoassay method was proposed for the simultaneous qualification of Salmonella typhimurium and Escherichia coli O157:H7 in food samples, showing high sensitivity and fast analysis. The silica-QD nanobead demonstrated superior fluorescence signal, monodispersity, and stability in sample detection, making it a promising tool for real bacterial sample detection.
Review
Chemistry, Analytical
Indrajit Patra, Mustafa M. Kadhim, Marwan Mahmood Saleh, Ghulam Yasin, Ali Abdulhussain Fadhil, Huda Sabah Jabr, Noora M. Hameed
Summary: This review provides an overview of the recent developments in aptasensors based on microfluidics for screening foodborne pathogenic bacteria and viruses. These sensors offer selectivity, sensitivity, portability, and affordability, and present a potential solution for improving on-site food safety detection platforms.
CRITICAL REVIEWS IN ANALYTICAL CHEMISTRY
(2022)
Review
Chemistry, Analytical
Hessamaddin Sohrabi, Afsaneh Hemmati, Mir Reza Majidi, Shirin Eyvazi, Ali Jahanban-Esfahlan, Behzad Baradaran, Roshanak Adlpour-Azar, Ahad Mokhtarzadeh, Miguel de la Guardia
Summary: This paper extensively explains the latest developments in portable biosensors and sensors for monitoring biological and chemical pollutants in water samples. It introduces different portable sensing assays and novel technologies in their development, as well as showcases various portable sensors developed for detecting different pollutants.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Article
Food Science & Technology
Guojian Wu, Huimin Qiu, Xin Liu, Pengjie Luo, Yongning Wu, Yizhong Shen
Summary: This review discusses the application of nanomaterials-based fluorescent assays in the detection of pathogenic bacteria. It highlights the progress in using functionalized nanoparticles for pathogenic bacteria detection. It emphasizes the advantages of nanomaterials-based fluorescent assays, such as shorter detection time and higher sensitivity compared to traditional methods.
TRENDS IN FOOD SCIENCE & TECHNOLOGY
(2023)
Article
Chemistry, Analytical
Daniel Torregrosa, Guillermo Grindlay, Miguel de la Guardia, Luis Gras, Juan Mora
Summary: Single particle inductively coupled plasma mass spectrometry (spICP-MS) has been used to determine metallic nanoparticles (NPs) in air. Different extraction strategies and extracting solvents were investigated, and a method for extracting metallic NPs from filters in 4 minutes was developed. The study also found that microquartz fiber filters had better NPs capturing performance. This method has been successfully applied to detect NPs in environmental monitoring stations.
Article
Food Science & Technology
Hasan Nasiri, Hamed Baghban, Reza Teimuri-Mofrad, Ahad Mokhtarzadeh
Summary: Graphitic carbon nitride supported on magnetic chitosan was synthesized and used as a support for cellobiose dehydrogenase immobilization and as a modifier for glassy carbon electrode in the fabrication of an electrochemical lactose sensor. The composite exhibited excellent electrochemical performance and was successfully applied in lactose quantification in dairy products.
INTERNATIONAL DAIRY JOURNAL
(2023)
Review
Immunology
Yousef Nami, Babak Haghshenas, Arash Javanmard, Mahya Samari, Nahid Mohammadi, Fatemeh Oroojalian, Ahad Mokhtarzadeh
Summary: The microbiome in the female reproductive tract is crucial for immune modulation and reproductive health. Imbalances in the microbiome during pregnancy can affect embryonic development and healthy births. Understanding the relationship between vaginal microbiota and reproductive outcomes is important for improving birth success. Microbiome dysbiosis refers to imbalances in the normal micro-biome due to the intrusion of pathogenic microorganisms. This review focuses on the natural uterine microbiome, mother-to-child transmission, dysbiosis, and microbial changes during pregnancy and parturition, as well as the effects of artificial uterus probiotics.
JOURNAL OF REPRODUCTIVE IMMUNOLOGY
(2023)
Review
Pathology
Nazila Mozammel, Mohammad Amini, Behzad Baradaran, Seyedeh Zahra Bahojb Mahdavi, Seyed Samad Hosseini, Ahad Mokhtarzadeh
Summary: MicroRNAs (miRNA) are a class of small non-coding RNAs that have significant impact on gene expression in human cancers. miR-145, a potential tumor suppressor miRNA, is downregulated in colorectal cancer and is involved in multiple processes such as cell proliferation, apoptosis, migration, and differentiation. It regulates tumor-specific signaling pathways, including KRAS and P53 signaling, through targeting various genes. This study aims to present recent findings on the functions of miR-145 and its involvement in colorectal cancer by interacting with multiple signaling pathways.
PATHOLOGY RESEARCH AND PRACTICE
(2023)
Article
Chemistry, Analytical
Payam Shahbazi-Derakhshi, Elham Mahmoudi, Mir Mostafa Majidi, Hessamaddin Sohrabi, Mohammad Amini, Mir Reza Majidi, Aligholi Niaei, Nima Shaykh-Baygloo, Ahad Mokhtarzadeh
Summary: In this research, a label-free electrochemical genosensing platform was developed for sensitive recognition of miRNA-21 in human gastric cell lines samples. The platform was based on the hybridization process using [Fe(CN)(6)](3-/4-) as a redox probe. Perovskite nanosheets were synthesized and combined with graphene oxide to create an effective electrochemical modifier. Gold Nano Particles (AuNPs) were immobilized on the modified electrode surface through chronoamperometry. The biomarker-detection bioassay exhibited high sensitivity and specificity, with a wide linear range for miRNA-21 detection.
Article
Genetics & Heredity
Noora Karim Ahangar, Mohammad Khalaj-Kondori, Nazila Alizadeh, Ahad Mokhtarzadeh, Amir Baghbanzadeh, Mahdi Abdoli Shadbad, Katayoun Dolatkhah, Behzad Baradaran
Summary: This study found that tumor-intrinsic HHLA2 knockdown increases the anti-tumoral effect of paclitaxel on MG-63 cells and enhances the chemosensitivity of MG-63 cells to paclitaxel.
Article
Oncology
Neda Rajabi, Fateme Mohammadnejad, Mohammad Amin Doustvandi, Mahdi Abdoli Shadbad, Mohammad Amini, Habib Tajalli, Ahad Mokhtarzadeh, Elham Baghbani, Nicola Silvestris, Behzad Baradaran
Summary: Photodynamic therapy (PDT) combined with tamoxifen (TA) has shown promising anti-cancer effects on MDA-MB-231 cells. The combination treatment increased cytotoxicity, induced apoptosis, inhibited migration, and suppressed clonogenicity. Furthermore, the synergy between ZnPc-PDT and TA enhanced the cytotoxicity and modulated gene expression.
PHOTODIAGNOSIS AND PHOTODYNAMIC THERAPY
(2023)
Article
Multidisciplinary Sciences
Mir-Hassan Moosavy, Miguel de la Guardia, Ahad Mokhtarzadeh, Seyed Amin Khatibi, Neda Hosseinzadeh, Nasser Hajipour
Summary: This study investigates the green synthesis and characterization of gold nanoparticles (AuNPs) and silver nanoparticles (AgNPs) using spearmint essential oil, as well as their antibacterial, antioxidant, and in vitro cytotoxic effects. The results show that the green synthesized AuNPs and AgNPs exhibit antibacterial, antioxidant, and in vitro cytotoxic activity.
SCIENTIFIC REPORTS
(2023)
Article
Multidisciplinary Sciences
Faranak Gholipour, Mohammad Amini, Behzad Baradaran, Ahad Mokhtarzadeh, Morteza Eskandani
Summary: Probiotic bacteria treated with curcumin showed unaffected anticancer properties, ability to combat pathogenic bacteria, and survival in acidic conditions. They exhibited stronger inhibitory effects on colon cancer cells compared to untreated bacteria, inducing cell apoptosis.
SCIENTIFIC REPORTS
(2023)
Article
Biochemical Research Methods
D. Gallart-Mateu, P. Dualde, C. Coscolla, J. M. Soriano, S. Garrigues, M. de la Guardia
Summary: The exposure to smoking related products was evaluated through the analysis of urine samples of smokers and vapers. The study identified and quantified various biomarkers and their metabolites in the urine samples. The findings suggest that traditional smoking practice is a major source of carcinogenic chemicals compared to substitutive practices, although there are still potential harm in alternative practices.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Sepideh Fathi, Nazila Jalilzadeh, Mohammad Amini, Dariush Shanebandi, Behzad Baradaran, Fatemeh Oroojalian, Ahad Mokhtarzadeh, Prashant Kesharwani, Amirhossein Sahebkar
Summary: Due to its high mortality rates, typhoid fever continues to be a major health problem worldwide, especially in developing countries. However, the lack of accurate diagnostic tests and the similarity of typhoid symptoms to other diseases pose a challenge in accurately diagnosing typhoid fever. To address this issue, researchers have developed a Surface Plasmon Resonance (SPR) based biosensor that can specifically detect Salmonella typhi through DNA hybridization. The results showed that the biosensor could detect synthetic target sequences at a concentration of 10 nM and PCR products at a concentration of 1 nM. Additionally, the biosensor was able to accurately distinguish between complementary sequences and sequences with one base mismatch. The chip's surface can be regenerated and reused for consecutive diagnoses using NaOH solution. These findings highlight the potential of the designed biosensor for the detection of Salmonella typhi.
ANALYTICAL BIOCHEMISTRY
(2023)
Review
Oncology
Pouriya Sameti, Maryam Tohidast, Mohammad Amini, Seyedeh Zahra Bahojb Mahdavi, Souzan Najafi, Ahad Mokhtarzadeh
Summary: Numerous studies have shown that miRNAs are dysregulated in various human cancers and play significant roles in tumorigenesis. In particular, the aberrant expression of miR-182 has been linked to malignancies. It functions as a dual-role regulator by directly or indirectly regulating the expression of numerous genes and modulating signaling pathways involved in tumor progression. This review highlights the effects of miR-182 dysregulation on the signaling pathways involved in tumorigenesis, emphasizing its potential as a therapeutic and diagnostic target.
CANCER CELL INTERNATIONAL
(2023)
Article
Engineering, Electrical & Electronic
Hassan Nasiri, Hamed Baghban, Reza Teimuri-Mofrad, Ahad Mokhtarzadeh
Summary: This study aims to develop a surface plasmon resonance (SPR) sensor using a Chitosan/Polyaniline/Graphene oxide-Ferrocene (CS/PANI/GOFc) composite thin film for glucose detection. The optimal composite formulation was determined based on experimental refractive index data and the effective medium theory (EMT). The prepared CS 25%/PANI 25%/GOFc 50% composite was evaluated in the design of the glucose sensor by modifying the SPR gold chip. The results show that the SPR sensor exhibits a linear response in the glucose concentration range of 0.5-100 μM with a sensitivity of 0.0202 μM-1. The binding affinity analysis using Langmuir and Sips models indicates that the composite film has a higher affinity constant compared to the bare gold chip.
OPTICAL AND QUANTUM ELECTRONICS
(2023)
Article
Pharmacology & Pharmacy
Farzaneh Bilan, Mohammad Amini, Mohammad Amin Doustvandi, Maryam Tohidast, Amir Baghbanzadeh, Seyed Samad Hoseini, Ahad Mokhtarzadeh, Behzad Baradaran
Summary: This study showed that the combination of miR-145 and anti-miR-21 had an anti-proliferative and pro-apoptotic effect on gastric cancer cells, and inhibited cell proliferation by modulating the PTEN/AKT1 signaling pathway. Therefore, the combination of miR-145 and anti-miR-21 may be a promising therapeutic approach for treating gastric cancer.
Article
Chemistry, Multidisciplinary
Payam Shahbazi-Derakhshi, Mohammad Abbasi, Amirhossein Akbarzadeh, Ahad Mokhtarzadeh, Hamid Hosseinpour, Jafar Soleymani
Summary: A novel electrochemical probe was developed for quantification of apixaban in unprocessed plasma samples using electrochemically oxidized graphene oxide aerogels and nano-sized Bi2Fe4O9 particles. The probe employed a ratiometric electrochemical method for enhanced specificity in plasma samples and utilized K-3[Fe(CN)(6)]/K-4[Fe(CN)(6)] as the redox pair for indirect detection of apixaban. Differential pulse voltammetry was used to record the current alteration of the probe, and the response was proportional to apixaban concentrations from 10 ng mL(-1) to 10 μg mL(-1) with a low limit of quantification of 10 ng mL(-1). The fabricated chemosensor showed high selectivity for detecting apixaban concentrations in unprocessed plasma samples due to the physical filtering antifouling activity of aerogels.
