Article
Biophysics
Xinxin Ke, Yangjing Ou, Yu Lin, Tao Hu
Summary: This study presents the first CRISPR/Cas12a-based chemiluminescence enhancement biosensor using HCR amplifying strategy for nucleic acids detection, showing high sensitivity and specificity, low-cost, and visual imaging advantages. The platform exhibits excellent sensitivity in detecting synthetic DNA targets, and achieves superior sensitivity and specificity in the practical application with HPV clinical samples.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Keyi Long, Gaihua Cao, Yue Qiu, Nannan Yang, Jian Chen, Mei Yang, Changjun Hou, Danqun Huo
Summary: In this study, we developed an HCR-Cas12a controller for sensitive detection of miR-21. The method exhibited a wide detection range and a low limit of detection. It also eliminates the need for expensive instruments, making it suitable for low-resource settings.
Article
Chemistry, Multidisciplinary
Long Ma, Dan Liao, Zhiying Zhao, Jun Kou, Haoyu Guo, Xin Xiong, Shuli Man
Summary: The study reports a versatile biosensing platform called SMART-Cas12a for non-nucleic acid small-molecule detection. The platform utilizes functional nucleic acid (aptamer) to trigger hybridization chain reaction cascade signal amplification, followed by integration of the CRISPR/Cas system to recognize and activate the trans-cleavage of the amplified products. This converts the target to nucleic acid signals, which can be visualized and analyzed using a customized 3D-printed visualizer and a home-made App-enabled smartphone.
Article
Biophysics
Jingjing Zhang, Chunyuan Song, Yunfeng Zhu, Hongyu Gan, Xinyue Fang, Qian Peng, Jingrong Xiong, Chen Dong, Caiqin Han, Lianhui Wang
Summary: In this study, a novel one-pot cascade signal amplification strategy integrating the CRISPR/Cas13a system and branched hybridization chain reaction (bHCR) was proposed for highly sensitive and specific SERS assay of disease-related nucleic acids. The method showed low detection limit, good linear calibration curve, and demonstrated good practicality in clinical samples.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Analytical
Mengyan Li, Nian Luo, Xiaofei Liao, Li Zou
Summary: We developed a dual signal amplification strategy for sensitive detection of circulating tumor DNA (ctDNA) based on hybridization chain reaction (HCR) and proximity hybridization-regulated CRISPR/Cas12a. This strategy exhibits high sensitivity toward ctDNA with a low detection limit of 5.43 fM, and it has been successfully applied for ctDNA detection in serum samples, showing great potential in the clinical diagnosis of cancer.
Article
Biophysics
Hai-Yan Jia, Hong-Li Zhao, Ting Wang, Pin-Ru Chen, Bin-Cheng Yin, Bang-Ce Ye
Summary: A versatile and sensitive miRNA detection platform based on CRISPR/Cas12a system and hybridization chain reaction (HCR) circuit was developed. The platform converts miRNA into activators through the HCR circuit, enabling sensitive detection of different miRNAs. The platform demonstrated consistency with RT-qPCR results and potential for clinical diagnosis of cancers and therapy monitoring.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Nanoscience & Nanotechnology
Qian Wang, Zuhao Zhang, Lu Zhang, Yunqing Liu, Li Xie, Shenguang Ge, Jinghua Yu
Summary: In this work, a CRISPR/Cas12a initiated switchable ternary electrochemiluminescence (ECL) biosensor combined with a Co3O4@Au nanoemitter is presented for the in vitro monitoring of miRNA-141. The biosensor demonstrated extraordinary sensitivity and specificity, with a low detection limit.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Analytical
Qiong Liu, He Zhang, Sainan Gao, Wenjie Ma, Xin Fu, Mei Yang
Summary: A novel colorimetric sensor combining CRISPR/Cas12a with hyperbranched hybridization chain reaction (HB-HCR) was developed for highly sensitive and specific diagnosis of Mycobacterium bovis. The sensor showed a very low limit of detection for Mycobacterium bovis target and demonstrated good stability and accuracy in real biological samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Multidisciplinary
Hermann Neitz, Claudia Hoebartner
Summary: We report the application of a fluorescent nucleoside called ToldU as a photoresponsive crosslinker in three different DNA architectures, resulting in enhanced fluorescence emission. The fluorogenic ToldU crosslinking reaction allows for the assembly of DNA polymers through a hybridization chain reaction, enabling the concentration-dependent detection of a specific DNA sequence. A new photoinduced fluorogenic interstrand crosslinking reaction is demonstrated in three different DNA architectures using the hydrophobic tolane-containing nucleoside, ToldU.
CHEMICAL COMMUNICATIONS
(2023)
Article
Chemistry, Analytical
Min Zhong, Jinbo Liu, Jie Wu, Jinqian Li, Nini Luo, Chuanlong Zhu, Rui Liu, Qianfeng Xia, Huangxian Ju
Summary: This study developed a dual signal amplification strategy for sensitive fluorescent detection of DENV RNA. The strategy utilized a target triggered hybridization chain reaction (HCR) and multiplex proximity ligation activated CRISPR-Cas12a to achieve dual signal amplification. The proposed assay demonstrated high sensitivity and accuracy, making it promising for clinical disease diagnosis.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Biophysics
Feng Qiu, Xiaoyan Gan, Jianglong Yao, Bingying Jiang, Ruo Yuan, Yun Xiang
Summary: In this study, a sensitive detection method for nuclear factor-kappa B p50 (NF-KB p50) was proposed by integrating hybridization chain reaction (HCR)-activated Cas12a enzyme with bio-responsive DNA hydrogels. The method achieved highly enhanced electrochemical signals for detecting target molecules at a low detection limit and successfully detected NF-KB p50 in lysate of HeLa cells.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Xuelan Wu, Ting Ju, Zeyang Li, Jingwen Li, Xingwei Zhai, Kun Han
Summary: This study designed and optimized a target-independent fluorescent signal system, Hybridization chain reaction Fluorescence resonance energy transfer (HCR-FRET) system. Combined with CRISPR/Cas12a system, a fluorescent biosensing protocol was developed for the detection of T790 M. The HCR-FRET system showed a detection range from 1 pM to 400 pM with a detection limit of 316 fM. The target-independent property of the HCR-FRET system indicates its potential for parallel detection of other DNA targets.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Physical
Jing Su, Yuqing Ke, Nokuzola Maboyi, Xiao Zhi, Sijia Yan, Fuwu Li, Bo Zhao, Xiaolong Jia, Shiping Song, Xianting Ding
Summary: The authors developed a CRISPR/Cas12a-powered DNA framework-supported electrochemical biosensing platform for ultrasensitive nucleic acid analysis without pre-amplification, showing incredible capability for sequence-specific targeting and detection.
Article
Agriculture, Multidisciplinary
Yayun Jiang, Chaochuan Zheng, Ming Jin, Ruolan Zhou, Qiaoli Wu, Fuyuan Huang, Yongliang Lou, Laibao Zheng
Summary: We developed a novel CRISPR/Cas12a mediated strand displacement/hybridization chain reaction (CSDHCR) nucleic acid assay for colorimetric detection of foodborne pathogens. The CSDHCR biosensor demonstrated satisfactory linear detection and high sensitivity for nucleic acid targets in the range of 10 fM to 1 nM, with a limit of detection of 4.54 fM. The method also showed promising specificity and sensitivity for the detection of Vibrio vulnificus, a foodborne pathogen, making it a potential alternative for ultrasensitive and visual detection of nucleic acids and foodborne pathogens.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Nanoscience & Nanotechnology
Hua Chai, Wenbo Cheng, Dayong Jin, Peng Miao
Summary: This article summarizes the assembly principles and applications of hybridization chain reaction (HCR) in DNA nanotechnology. HCR has excellent assembly kinetics and ease of operation, making it suitable for ultra-sensitive detection of various targets. Future advancements in overcoming challenges are needed for further development in this field.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Chemistry, Multidisciplinary
Yu Zheng, Tao Hu, Chuanxia Chen, Fan Yang, Xiurong Yang
CHEMICAL COMMUNICATIONS
(2015)
Article
Nanoscience & Nanotechnology
Shasha Lu, Tao Hu, Shuang Wang, Jian Sun, Xiurong Yang
ACS APPLIED MATERIALS & INTERFACES
(2017)
Article
Chemistry, Analytical
Jian Sun, Tao Hu, Chuanxia Chen, Dan Zhao, Fan Yang, Xiurong Yang
ANALYTICAL CHEMISTRY
(2016)
Article
Chemistry, Multidisciplinary
Jian Sun, Tao Hu, Xiaolong Xu, Lei Wang, Xiurong Yang
Article
Chemistry, Analytical
Tao Hu, Chuanxia Chen, Guangming Huang, Xiurong Yang
SENSORS AND ACTUATORS B-CHEMICAL
(2016)
Article
Chemistry, Analytical
Tao Hu, Shasha Lu, Chuanxia Chen, Jian Sun, Xiurong Yang
SENSORS AND ACTUATORS B-CHEMICAL
(2017)
Article
Multidisciplinary Sciences
Meng Liang, Wenqing Li, Hui Tian, Tao Hu, Lu Wang, Yu Lin, Yulei Li, Hefeng Huang, Fei Sun
SCIENTIFIC REPORTS
(2014)
Review
Biochemistry & Molecular Biology
Xinxin Ke, Tao Hu, Mizu Jiang
Summary: The cGAS-STING signaling pathway plays a crucial role in GI inflammatory diseases and cancers. Understanding the function and mechanism of this pathway is helpful for developing therapeutic strategies.
Article
Chemistry, Analytical
Tao Hu, Xinxin Ke, Yangjing Ou, Yu Lin
Summary: In this study, a CRISPR/Cas12a-triggered chemiluminescence enhancement biosensor named CRICED was developed for ultrasensitive nucleic acid detection. The biosensor showed high sensitivity and specificity in detecting synthetic DNA targets, amplified DNA plasmids, and clinical samples, demonstrating its potential for practical applications in CRISPR-based diagnostics.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Tao Hu, Xinxin Ke, Wei Li, Yu Lin, Ajuan Liang, Yangjing Ou, Chuanxia Chen
Summary: CRISPR-RDB is an affordable and sensitive multiplex target detection method that combines CRISPR-Cas12a with a reverse dot blot technique. It can simultaneously detect multiple targets and convert the results into visual color signals. This method can not only rapidly detect pathogens such as influenza viruses, respiratory syncytial virus, and SARS-CoV-2, but also be modified for detection of other targets with simple modifications.
Article
Chemistry, Analytical
Xinxin Ke, Yi Hu, Chuanxia Chen, Tao Hu
Summary: In this study, a one-tube dual-readout biosensor (CRISAT) was developed using CRISPR-ALP tandem assay for the detection of ultrasensitive nucleic acids and non-nucleic acids. By activating Cas12a to cut the hyDNA sequence, abundant ALP is released, leading to the generation of Si PCDs or PCDs. This method offers a fluorescence and colorimetry dual-readout nanoplatform for CRISPR-based biosensors with high sensitivity and specificity.
Article
Chemistry, Analytical
Hu Tao, Yang Fan, Yang Xiu-Rong
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
(2017)
Article
Chemistry, Analytical
Tao Hu, Shuang Wang, Chuanxia Chen, Jian Sun, Xiurong Yang
ANALYTICAL CHEMISTRY
(2017)
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)