4.7 Article

Structural properties, antioxidant and immune activities of low molecular weight peptides from soybean dregs (Okara)

期刊

FOOD CHEMISTRY-X
卷 12, 期 -, 页码 -

出版社

ELSEVIER
DOI: 10.1016/j.fochx.2021.100175

关键词

High-pressure homogenization; Double enzyme hydrolysis; Okara peptides; Immunity; Antioxidation

资金

  1. China Agriculture Research System of MOF and MARA [CARS-04]

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A method for preparing low molecular weight peptides from okara using high-pressure homogenization assisted double enzymes was proposed in this study, showing increased protein extraction rate and positive immunological effects on cells. This method is effective for industrial preparation of okara peptides and promotes the reuse of okara resources.
In this study, a method for preparing low molecular weight peptides (HPH-VAP) from okara using high-pressure homogenization assisted double enzymes was proposed. In order to explore its advantages, the effects of various methods on protein extraction rate and on the structure, antioxidant and immune properties of peptides were compared. The results showed that the protein extraction rate of this method was increased by 69% and 51% compared with other methods, and the structure only led to changes in the hydrogen bonds between peptide chains. HPH-VAP was screened out through functional characteristics, its structure was identified by HPLC-MS/ MS, and further immunological activity analysis was carried out. The results showed that it promoted cell phagocytic ability, NO level and release of cytokines IL-6, IFN-gamma, TNF-alpha. Therefore, this method is an effective and applicable method for industrial preparation of okara peptides, and has a positive effect on the reuse of okara resources.

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