Article
Chemistry, Analytical
Milad Tavassoli, Arezou Khezerlou, Hamed Hamishehkar, Ali Ehsani, Balal Khalilzadeh
Summary: An innovative aptamer labeled with 5-FAM and using graphene oxide (GO) as a quenching platform has been developed for high affinity detection of Y. enterocolitica. The system showed selectivity towards Y. enterocolitica in the presence of other common bacteria. Optimized conditions resulted in a wide linear response range and a low limit of detection, making the system potentially useful for rapid screening of Y. enterocolitica.
Article
Biochemistry & Molecular Biology
Marta Nieckarz, Karolina Jaworska, Adrianna Raczkowska, Katarzyna Brzostek
Summary: This study investigates the role of the transcriptional regulator OmpR in membrane protein expression and secretion in Yersinia enterocolitica. The findings show that OmpR negatively affects the levels of Ysc-Yop proteins, particularly YopD, and represses the expression of yopD. OmpR also has a positive role in the expression of the master regulator of the flagellar-associated T3SS, flhDC.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Bruna Torres Furtado Martins, Everton Cruz de Azevedo, Ricardo Seiti Yamatogi, Douglas Ruben Call, Luis Augusto Nero
Summary: The study identified the persistence of a clonal Yersinia enterocolitica bio-serotype 4/O:3 in a particular pork production chain in Brazil, with isolates carrying various virulence genes and multidrug resistance related genes.
Article
Biochemistry & Molecular Biology
Amit Kumar, Shruti Ahlawat, Hari Mohan, Krishna Kant Sharma
Summary: Laccases are polyphenol multicopper oxidases known for their structural and functional diversity in various life forms. The study compared the molecular and physico-chemical properties of fungal laccase isoforms from Ganoderma lucidum with recombinant bacterial laccases from Yersinia enterocolitica strains. Results showed differences in the conserved residues and stability of FLIs compared to rLac under different conditions. These findings provide insights into the evolution of catalytic efficiency and redox properties of FLIs.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemistry & Molecular Biology
Jens A. Hammerl, Andrea Barac, Philipp Erben, Julius Fuhrmann, Ashish Gadicherla, Franziska Kumsteller, Anne Lauckner, Felix Mueller, Stefan Hertwig
Summary: Researchers isolated and characterized two phages from game animals, which showed a broad host range and effectively lysed various serotypes of Yersinia enterocolitica. These phages also demonstrated lytic activity against different strains of Yersinia pseudotuberculosis under specific conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Medicine, Research & Experimental
Joao Victor Silva-Silva, Carla J. Moragas-Tellis, Maria S. S. Chagas, Paulo Victor R. Souza, Davyson L. Moreira, Celeste S. F. de Souza, Kerolain F. Teixeira, Arthur R. Cenci, Aldo S. de Oliveira, Fernando Almeida-Souza, Maria D. Behrens, Katia S. Calabrese
Summary: This study evaluated the leishmanicidal activity of a crude extract and anthocyanidin-rich fractions from Arrabidaea chica, as well as three isolated anthocyanidins. The results showed promising anti-leishmanial effects of these compounds, indicating their potential as therapeutic agents for leishmaniasis.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Biochemistry & Molecular Biology
Achini K. Vidanapathirana, Jarrad M. Goyne, Anna E. Williamson, Benjamin J. Pullen, Pich Chhay, Lauren Sandeman, Julien Bensalem, Timothy J. Sargeant, Randall Grose, Mark J. Crabtree, Run Zhang, Stephen J. Nicholls, Peter J. Psaltis, Christina A. Bursill
Summary: This study demonstrates the uptake and detection capacity of a ruthenium-based fluorescent NO sensor (Ru-NO) in macrophages, plasma, and atherosclerotic plaques, suggesting its potential utility as a sensor of atherosclerosis.
Article
Microbiology
Philip A. Karlsson, Eva Tano, Cecilia Jernberg, Rachel A. Hickman, Lionel Guy, Josef D. Jarhult, Helen Wang
Summary: The study reveals that the Y. enterocolitica outbreak strains in Sweden possess multi-antibiotic and metal-resistance genes acquired through horizontal gene transfer. Additionally, the bacteria were found to carry Tn2670 and Pasteurellaceae plasmids. These findings suggest a potential reservoir of intraspecies dissemination of multidrug-resistance genes among foodborne pathogens.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Jens Andre Hammerl, Sabrin El-Mustapha, Michelle Boelcke, Hannah Trampert, Andrea Barac, Claudia Jaeckel, Ashish K. Gadicherla, Stefan Hertwig
Summary: The study identified three groups of temperate phages in pathogenic Y. enterocolitica strains, each with different lytic activity against different strains.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Aleksandra Platt-Samoraj, Klaudia Konczyk-Kmiecik, Tadeusz Bakula
Summary: The study found a high prevalence of Y. enterocolitica among commensal rodents, with all isolates classified as biotype 1A and sporadic isolation of Y. pseudotuberculosis, indicating no high epidemiological risk.
Article
Food Science & Technology
Lu Tian, Jiapeng Fu, Mi Wu, Sichen Liao, Xinyi Jia, Jie Wang, Siqi Yang, Zhiqiang Liu, Zhijun Liu, Zhifei Xue, Yujiao Wang, Hui Li, Guoli Gong
Summary: This study examines the membrane damage mechanism of gallic acid on Yersinia enterocolitica and explores its potential to prolong the shelf life of pork. Findings suggest that gallic acid can be used as a food preservative to inhibit the growth of Yersinia enterocolitica and extend the shelf life of pork.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2022)
Article
Chemistry, Applied
Lu Tian, Xuyang Wang, Di Zhang, Mi Wu, Zhifei Xue, Zhiqiang Liu, Siqi Yang, Hui Li, Guoli Gong
Summary: This study investigated the antibacterial mechanism of protocatechualdehyde (PCA) against Yersinia enterocolitica, confirming its effectiveness in pork preservation by inhibiting bacterial growth and reproduction.
Article
Biochemistry & Molecular Biology
Anju Thapa, Ravinder Kaushik, Smriti Arora, Sundeep Jaglan, Varun Jaswal, Virendra Kumar Yadav, Manjeet Singh, Aarti Bains, Prince Chawla, Azhar Khan, Melinda Fogarasi, Szabolcs Fogarasi
Summary: Yersiniosis, a zoonotic disease caused by Yersinia enterocolitica, is prevalent in Europe and known for its high antibiotic resistance. This study evaluated the antimicrobial activity of an extract from Picrorhiza kurroa against Yersinia enterocolitica. The extract showed effective antimicrobial activity and potential bioactive compounds were identified. Picroside-1 exhibited higher antimicrobial potential and lysed Yersinia enterocolitica cells. Docking analysis revealed its interaction with the dihydrofolate reductase protein, suggesting it as a possible drug candidate for Yersinia enterocolitica.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Can Huang, Wenqian Li, Jingyu Chen
Summary: This study aims to investigate the function of DksA in fatty acid metabolism and cell membrane structure in Yersinia enterocolitica. The results showed that deletion of DksA resulted in decreased expression of fatty acid degradation genes and slower growth at low temperatures. Furthermore, DksA positively regulated the integrity of the inner and outer membranes of Y. enterocolitica under polymyxin B exposure.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Food Science & Technology
Juan Wang, Ming Liu, Huixian Wang, Qingping Wu, Yu Ding, Tianxiang Xu, Guoxiang Ma, Yueming Zhong, Jumei Zhang, Moutong Chen, Liang Xue, Qinghua Ye, Haiyan Zeng, Xiaojuan Yang, Runshi Yang
Summary: This study investigated the incidence of Yersinia enterocolitica strains in food samples in China, revealing a low contamination rate, predominance of biotype 1A and serotype O:5, sensitivity to tetracycline, trimethoprim/sulfamethoxazole, and chloramphenicol, and resistance to ampicillin and amoxicillin/clavulanic acid. The strains were divided into seven clusters based on their genetic characteristics. Additionally, the study highlighted the potential risk of Y. enterocolitica contamination associated with stringent cold storage methods.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Fatima Ferragut, Alejandro J. Cagnoni, Lucas L. Colombo, Clara Sanchez Terrero, Carlota Wolfenstein-Todel, Maria F. Troncoso, Silvia I. Vanzulli, Gabriel A. Rabinovich, Karina V. Marino, Maria T. Elola
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2019)
Article
Biochemical Research Methods
Elisha Goonatilleke, Jennifer T. Smilowitz, Karina Marino, Bruce J. German, Carlito B. Lebrilla, Mariana Barboza
MOLECULAR & CELLULAR PROTEOMICS
(2019)
Article
Biochemistry & Molecular Biology
Sebastian M. Maller, Alejandro J. Cagnoni, Nadia Bannoud, Lorena Sigaut, Juan M. Perez Saez, Lia I. Pietrasanta, Ri-Yao Yang, Fu-Tong Liu, Diego O. Croci, Santiago Di Lella, Victoria Sundblad, Gabriel A. Rabinovich, Karina V. Marino
Review
Biochemistry & Molecular Biology
Alejandro J. Cagnoni, Maria F. Troncoso, Gabriel A. Rabinovich, Karina V. Marino, Maria T. Elola
BIOCHEMICAL SOCIETY TRANSACTIONS
(2020)
Editorial Material
Chemistry, Multidisciplinary
Matthew S. Macauley, Christoph Rademacher, Karina V. Marino
FRONTIERS IN CHEMISTRY
(2020)
Article
Biochemical Research Methods
Alejandro J. Cagnoni, Emiliano D. Primo, Sebastian Klinke, Maria E. Cano, Walter Giordano, Karina V. Marino, Jose Kovensky, Fernando A. Goldbaum, Maria Laura Uhrig, Lisandro H. Otero
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Rodrigo Papa-Gobbi, Cecilia I. Muglia, Andres Rocca, Renata Curciarello, Alicia M. Sambuelli, Martin Yantorno, Gustavo Correa, Luciano G. Morosi, Antonio Di Sabatino, Paolo Biancheri, Thomas T. MacDonald, Marta A. Toscano, Karina V. Marino, Gabriel A. Rabinovich, Guillermo H. Docena
Summary: The study found that transient regulation of O-glycan profile during inflammation modulates Gal-1 binding and LP T-cell survival in IBD patients.
Article
Multidisciplinary Sciences
Alejandro J. Cagnoni, Maria Laura Giribaldi, Ada G. Blidner, Anabela M. Cutine, Sabrina G. Gatto, Rosa M. Morales, Mariana Salatino, Martin C. Abba, Diego O. Croci, Karina V. Marino, Gabriel A. Rabinovich
Summary: Colorectal cancer is a common malignancy with limited response to immunotherapy, prompting the need for new biomarkers and therapeutic targets. Gal-1, as an endogenous glycan-binding protein, has been found to influence the activity of CD8(+) regulatory T cells in CRC, suggesting a potential immunotherapeutic approach for this disease.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Immunology
Nadia Bannoud, Tomas Dalotto-Moreno, Lucia Kindgard, Pablo A. Garcia, Ada G. Blidner, Karina V. Marino, Gabriel A. Rabinovich, Diego O. Croci
Summary: This study highlights the reciprocal regulation between hypoxia, angiogenesis, and immunosuppression in tumor progression and anti-tumor therapies. The findings suggest a rational basis for optimizing synergistic combinations of antiangiogenic and immunotherapeutic strategies to enhance treatment efficacy.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Cecilia Porciuncula-Gonzalez, Alejandro J. Cagnoni, Carolina Fontana, Karina Marino, Patricia Saenz-Mendez, Cecilia Giacomini, Gabriela Irazoqui
Summary: This study evaluated the binding affinity of Gal-1 for various galactosides, showing weaker affinity for beta-(1->6) galactosides compared to beta-(1->4) and beta-(1->3) galactosides. Docking experiments revealed key interactions between Gal-1 and specific amino acid residues of evaluated galactosides, contributing to the understanding of galectin-glycan affinity.
BIOORGANIC & MEDICINAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Luciano G. Morosi, Anabela M. Cutine, Alejandro J. Cagnoni, Montana N. Manselle-Cocco, Diego O. Croci, Joaquin P. Merlo, Rosa M. Morales, Maria May, Juan M. Perez-Saez, Maria R. Girotti, Santiago P. Mendez-Huergo, Betiana Pucci, Anibal H. Gil, Sergio P. Huernos, Guillermo H. Docena, Alicia M. Sambuelli, Marta A. Toscano, Gabriel A. Rabinovich, Karina Marino
Summary: The study reveals a glycosylation-dependent on-off circuit controlled by Galectin-1 (Gal1) and its glycosylated ligands which target activated CD8(+) T cells and shape cytokine profile to maintain intestinal homeostasis. In patients with inflammatory bowel disease, increased Gal1 is associated with dysregulated expression of glycosyltransferases C2GNT1 and ST6GAL1. This provides insights into the role of Gal1 and its glycosylated ligands in recalibrating T cell immunity to preserve intestinal homeostasis.
Article
Oncology
Valeria da Costa, Sandra J. van Vliet, Paula Carasi, Sofia Frigerio, Pablo A. Garcia, Diego O. Croci, Maria Florencia Festari, Monique Costa, Mercedes Landeira, Santiago A. Rodriguez-Zraquia, Alejandro J. Cagnoni, Anabela M. Cutine, Gabriel A. Rabinovich, Eduardo Osinaga, Karina Marino, Teresa Freire
Summary: Tn antigen, originating from tumor cells, interacts with the MGL receptor to promote immunosuppression and angiogenesis, ultimately favoring tumor progression.
Article
Cell Biology
M. Constanza Baroni Pietto, Paola R. Lev, Ana C. Glembotsky, Cecilia P. Marin Oyarzun, Graciela Gomez, Victoria Collado, Cecilia Pisoni, Ramiro A. Gomez, Matias Grodzielski, Jacqueline Gonzalez, Karina Marino, Paula G. Heller, Nora P. Goette, Rosana F. Marta
Summary: The study found that systemic lupus erythematosus (SLE) patients with thrombocytopenia have abnormalities in platelet clearance and production, mainly due to apoptosis and desialylation leading to low platelet count. These abnormalities are more frequently observed in patients with thrombocytopenia and active disease.
Review
Biotechnology & Applied Microbiology
Karina V. Marino, Alejandro J. Cagnoni, Diego O. Croci, Gabriel A. Rabinovich
Summary: This article discusses the therapeutic relevance of galectins to cancer and fibrosis, and reviews the various strategies to target galectins and their progress in clinical trials.
NATURE REVIEWS DRUG DISCOVERY
(2023)