期刊
INTERNATIONAL JOURNAL OF FOOD SCIENCES AND NUTRITION
卷 67, 期 2, 页码 111-116出版社
TAYLOR & FRANCIS LTD
DOI: 10.3109/09637486.2016.1144722
关键词
ACE-inhibitory peptide; Caco-2 cell monolayer; digestion; egg; transport
资金
- National Natural Science Foundation of China [3,1,2,71907, 3,1,471597]
- Graduate Innovation Fund of Jilin University [20,1,5123]
The objective of this study was to investigate the digestion and absorption of egg white-derived angiotensin I-converting enzyme (ACE)-inhibitory peptide TNGIIR in human intestinal Caco-2 cell monolayers. Results showed that the digestion of TNGIIR to simulated gastrointestinal enzymes and brush border membrane peptidases were 5.87% +/- 1.92% and 17.17% +/- 0.64%, respectively (p<0.05). The apparent permeability coefficients (P-app) of TNGIIR from the apical to basolateral side in Caco-2 cell monolayers was determined to be (4.92 +/- 0.40) x 10(-6) cm/s, indicating that TNGIIR can transport across Caco-2 cell monolayers in intact form. In addition, only cytochalasin D, a disruptor of tight junctions (TJs), changed TNGIIR transport rate significantly (p<0.05), suggesting that the main transport route for TNGIIR across Caco-2 cell monolayers was paracellular pathway via TJs.
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