Article
Multidisciplinary Sciences
Peibo Wang, Yongcan Zhou, Bei Wang, Ahmed Elaswad, Shifeng Wang, Weiliang Guo, Dongdong Zhang
Summary: This study presents an optimized protocol for preparing high-quality single-cell suspensions from the intestine of Nile tilapia using a combination of collagenase/dispase enzymes. The protocol is effective for dissociation, reduces cell aggregation, and provides sufficient viable cells for single-cell sequencing. It can also be adapted for other fish species.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2023)
Article
Fisheries
Weisheng Wang, Chuanyan Pan, Min Lv, Qiufeng Ruan, Weijie Chen, Laiba Shafique, Shakeela Parveen, Zheng Liang, Huawei Ma, Xu Luo
Summary: The study revealed that Hb toxicity inhibited NTK cell growth, upregulated iron metabolism and inflammation-related gene expression, activated inflammatory cytokines and antioxidant enzyme genes through NF-kappa B pathway, increased intracellular iron and ROS levels, and induced expression of apoptosis-related genes in NTK cells.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Shun Maekawa, Theeraporn Pulpipat, Pei-Chi Wang, Shih-Chu Chen
Summary: The study investigated the gene expression responses to Fno infection in Nile tilapia by analyzing the transcriptome of the head kidney and spleen. Differentially expressed genes were significantly enriched in immune-related pathways. Additionally, intracellular iron storage in the spleen causing anemia of inflammation after Fno infection was observed.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Fisheries
Yisha Yan, Rui Dong, Chaoyi Zhang, Quan Jiang
Summary: This study provides the first evidence that interleukin-6 (IL-6) mediates the inhibition of irisin secretion by lipopolysaccharide (LPS) through activation of the JAK2/STAT3 signaling pathway.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Xun Liu, Tian-Tian Tian, Xu-Jie Zhang, Yan-Ling Song, Yuan-Yuan Yao, Jian-Min Ye, Yong-An Zhang
Summary: In this study, a specific monoclonal antibody against Nile tilapia Ig lambda was prepared, which could recognize Ig lambda in serum and Ig lambda(+) B cells in tissues. The composition of IgM(+) and Ig lambda(+) B cell subsets was analyzed using this antibody and a mouse anti-tilapia IgM heavy chain antibody. The antibody level after immunization of Nile tilapia can be detected using this antibody, which lays a foundation for the evaluation of immunization effect of tilapia vaccine.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Neeraj Sood, Dev Kumar Verma, Anutosh Paria, Shrish Chandra Yadav, Manoj Kumar Yadav, Megha Kadam Bedekar, Saurav Kumar, Thangaraj Raja Swaminathan, Chadag Vishnumurthy Mohan, K. Rajendran, Pravata Kumar Pradhan
Summary: The study compared the transcriptional profiles of liver in experimentally-infected and control tilapia, revealing the host's attempt to resist TiLV infection through various signaling pathways. However, upregulation and downregulation of key genes indicated TiLV's ability to subvert the host immune response successfully.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Oliver Vocking, J. K. Famulski
Summary: In this study, single cell RNA sequencing revealed that anterior segment mesenchyme (ASM) differentiates into corneal epithelium/endothelium/stroma or annular ligament (AL) cell lineages as early as 48 hpf. Clustering analysis indicates common developmental pathways for corneal endothelium/stroma and AL, and distinct pathways for corneal epithelium starting at 48 hpf. The spatial and temporal validation of over 80 genes associated with anterior segment (AS) development demonstrated high conservation with mammalian trabecular meshwork and corneal tissues.
SCIENTIFIC REPORTS
(2023)
Article
Environmental Sciences
Sadia Sharmin, Md Touhidul Islam, Md Anwar Sadat, Rayeda Jannat, Md Rushna Alam, Md Shahjahan
Summary: The study revealed that exposure to the pesticide sumithion led to significant changes in hematological parameters of Nile tilapia, including decreased red blood cell and hemoglobin levels, and increased white blood cell and blood glucose levels. Additionally, substantial histopathological alterations were observed in the sumithion-treated fish, indicating a negative impact on Nile tilapia.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Fisheries
Samantha Chung, Karina Ribeiro, Jose Fernando Bibiano Melo, Daniel Vitor Teixeira, Luiz Vitor Oliveira Vidal, Carlos Eduardo Copatti
Summary: Supplementation with 0.5 mL EOZO kg diet(-1) improved growth performance, intestinal villi size, and hematological variables in Nile tilapia without compromising metabolism and hepatic histomorphometry.
Article
Fisheries
Kunming Li, Jiahua Zhu, Kang Li, Wei Liang, Jiansong Zhang, Qian Zhang, Xinying Jiao, Xiaodan Wang, Xiumei Wei, Jialong Yang
Summary: High-fat diet impairs lymphocyte homeostasis and T-cell-mediated adaptive immune response in tilapia, which may be associated with abnormal lipid metabolism in lymphocytes.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2022)
Article
Fisheries
Siwen Zheng, Wen-Xiong Wang
Summary: The functional heterogeneity of fish gills at the single cell scale is important. This study used single cell RNA sequencing to identify 12 cell populations in the gills of Oreochromis niloticus fish and analyzed their functional heterogeneity. The expression patterns of genes encoding ion transporters in different cell populations were examined, as well as the immune cell subtypes. The results provide insights into the physiological and immune responses of fish gills at the cellular level.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Marine & Freshwater Biology
Zhi-Hua Li, Shaoying Xing, Ping Li, Shuwen He, Zhihan Cao, Xu Wang, Xuqian Cao, Bin Liu, Hong You
Summary: In this study, the toxicological effects of triphenyltin (TPT) in Nile tilapia under different salinities were investigated using biochemical, histological, and transcriptional analyses. The results showed that TPT exposure weakened antioxidant defenses and caused liver damage, with a primary impact on lipid metabolism and immunity. Salinity exposure alone primarily affected carbohydrate metabolism, while combined exposure affected immune- and metabolic-related signaling pathways. Furthermore, single exposure to TPT or salinity induced inflammation by up-regulating pro-inflammatory cytokines, while combined exposure suppressed inflammation by down-regulating pro-inflammatory cytokine levels. These findings enhance our understanding of the negative effects of TPT exposure in Nile tilapia in various salinity zones and their potential defense mechanisms.
AQUATIC TOXICOLOGY
(2023)
Article
Infectious Diseases
Henrique Cesar Pereira Figueiredo, Guilherme Campos Tavares, Fernanda Alves Dorella, Julio Cesar Camara Rosa, Sostenes Apolo Correia Marcelino, Felipe Pierezan, Felipe Luiz Pereira
Summary: This study reports the first fatal ISKNV infections in Brazilian Nile tilapia fish farms, along with other bacterial infections, posing a new challenge to the aquaculture sector in the country.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Biochemistry & Molecular Biology
Jin-Mi Oh, Minae An, Dae-Soon Son, Jinhyuk Choi, Yong Beom Cho, Chang Eun Yoo, Woong-Yang Park
Summary: There are differences in the proportions of cell types between single-cell RNA sequencing (scRNA-seq) and single-nucleus RNA sequencing (snRNA-seq). The proportion of immune cells is relatively high in scRNA-seq, while the proportion of epithelial cells in the colon and hepatocytes in the liver is relatively high in snRNA-seq.
EXPERIMENTAL AND MOLECULAR MEDICINE
(2022)
Article
Veterinary Sciences
Huan Zhong, Chenyi Lou, Bingxin Ren, Jiamin Pi, Tao Dai, Weiling Qin, Yi Zhou
Summary: This study investigates the effect of ghrelin on the transcriptomic profile of tilapia liver and reveals that ghrelin promotes energy metabolism and RNA transcription in the liver through regulation of the ghrelin-growth hormone secretagogue receptor (GHS-R) and growth hormone/insulin-like growth factor-1 (GH/IGF-1) axes, providing new insights for the growth of tilapia.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Fisheries
Minxuan Yang, Zhijie Lu, Fenglin Li, Fei Shi, Fanbin Zhan, Yulei Zhang, Lijuan Zhao, Yanan Li, Jun Li, Li Lin, Zhendong Qin
Summary: The study showed that alginate oligosaccharide (AOS) can enhance growth performance, promote fat metabolism, and improve antioxidant capacity in grass carp. Transcriptomic analysis revealed that AOS activates pathways related to fatty metabolism and enhances the expression of genes associated with fat metabolism.
Article
Fisheries
Zhijie Lu, Meizhen Tang, Yanan Li, Fei Shi, Fanbin Zhan, Menglan Zhang, Lijuan Zhao, Jun Li, Li Lin, Zhendong Qin
Summary: The Fas-associated protein with Death Domain (FADD) plays a significant role in innate immunity and antibacterial immune responses in teleost fish. The over-expression of GcFADD in CIK cells activated the significant expression of apoptosis-related genes, indicating its involvement in cell death regulation.
Article
Fisheries
Yanan Li, Xiaolong Qiu, Zhijie Lu, Fanbin Zhan, Minxuan Yang, V. Sarath Babu, Jun Li, Zhendong Qin, Li Lin
Summary: In this study, the MST2 gene of grass carp was cloned and its tissue distribution was analyzed. CiMST2 was highly expressed in brain tissue and significantly up-regulated under bacterial infection, indicating its involvement in the immune response. CiMST2 regulated host redox homeostasis via Nrf2-ARE signaling, providing insights for disease prevention and control in the grass carp farming industry.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Fisheries
Abeer M. Hegazy, Nan Chen, Hanzuo Lin, Sarath Babu, Feng Li, Youcheng Yang, Zhendong Qin, Fei Shi, Jun Li, Li Lin
Summary: The study investigated the regulatory potential of apoptosis signals during SHVV infection, revealing that viral M protein induces apoptosis through the intrinsic apoptotic pathway.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Fisheries
Chong Wang, Shuang Liu, Tingting Xu, Xiaoping Li, Jun Li, Qingli Zhang
Summary: The study established an in vivo model of CMNV infection in zebrafish and found that infected individuals exhibited abnormal behavior and protruding eyes. CMNV was confirmed to be present in eye and brain tissues, as well as in muscle, intestine, and gill tissues. Additionally, CMNV caused a mortality rate of 53.33% in zebrafish within 14 days post challenge injection, highlighting the importance of further research on host-virus interactions and molecular mechanisms of CMNV.
Article
Fisheries
Zhijie Lu, Menglan Zhang, Meizhen Tang, Yanan Li, Fei Shi, Fanbin Zhan, Lijuan Zhao, Jun Li, Li Lin, Zhendong Qin
Summary: The study reveals that overexpression of HO-1 can decrease inflammatory cytokine secretion, inhibit pro-apoptotic gene expression, and depend on NF-kappa B, Fas, TNF-alpha death receptor, and mitochondrial pathway inhibition, as well as Nrf2-Keap1 and p38 MAPKs activation. These findings demonstrate the significant role of HO-1 overexpression in cell survival.
Article
Fisheries
Zhijie Lu, Meizhen Tang, Menglan Zhang, Yanan Li, Fei Shi, Fanbin Zhan, Lijuan Zhao, Jun Li, Li Lin, Zhendong Qin
Summary: In this study, the BAD gene GcBAD1 was cloned and characterized for the first time in grass carp, showing widespread expression in various tissues and significant up-regulation during infection. Overexpression of GcBAD1 also activated apoptosis-related genes and played a role in the mitochondrial apoptosis pathway of grass carp's innate immunity.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2021)
Article
Fisheries
Zhijie Lu, Meizhen Tang, Menglan Zhang, Yanan Li, Fei Shi, Fanbin Zhan, Lijuan Zhao, Jun Li, Li Lin, Zhendong Qin
Summary: Excess heme and Hb can amplify the secretion of inflammatory cytokines, leading to cytotoxicity and pro-inflammatory responses, providing potential strategies for immune intervention.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2022)
Article
Immunology
Youcheng Yang, Jiajun Chen, Linqing Lu, Zizheng Xu, Feng Li, Minxuan Yang, Jun Li, Li Lin, Zhendong Qin
Summary: Studies have shown that goose erythrocytes have the ability to phagocytose and exhibit antimicrobial activity. They can produce reactive oxygen species and inducible nitric oxide synthase in response to bacterial stimulation. Furthermore, goose erythrocytes show powerful antibacterial activity and up-regulate the expression of inflammatory factors and antioxidant enzymes.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Zhendong Qin, Minxuan Yang, Zhijie Lu, V. Sarath Babu, Yanan Li, Fei Shi, Fanbin Zhan, Chun Liu, Jun Li, Li Lin
Summary: Intravascular hemolysis leads to accumulation of hemoglobin and iron, activation of antioxidant system, and tissue oxidative damage in grass carp. The study suggests that excess of released Hb from hemolysis may be associated with ROS and inflammation generation.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Fisheries
Mengru Jin, Jiaojiao He, Jun Li, Yonghua Hu, Dongmei Sun, Hanjie Gu
Summary: The study reveals that YccA is a novel virulence factor of E. piscicida and essential for bacterial pathogenicity through evasion of the host's innate immune functions.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Immunology
Zi-You Ma, Jia-Xin Liang, Wen-Shuo Li, Yuan Sun, Chang-Song Wu, Ya-Zhen Hu, Jun Li, Yong-An Zhang, Xu-Jie Zhang
Summary: This study uncovered the interaction between the complement system and phagocytic B cells in teleost fish, and revealed the stimulating function of C3a on the phagocytic activity of IgM(+) B cells.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Environmental Sciences
Tingting Xu, Yuding Fan, Tianchang Jia, Chong Wang, Wei Wang, Jun Li, Qingli Zhang, Cuiluan Yao
Summary: An epidemiological survey was conducted in coastal China to investigate the prevalence and susceptibility of large yellow croaker Larimichthys crocea to Covert mortality nodavirus (CMNV). The study found that CMNV infection rates were relatively high in cultured L. crocea and may have significant impacts on the species, warranting further research.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Fisheries
Zhendong Qin, V. Sarath Babu, Yanan Li, Fei Shi, Fanbin Zhan, Chun Liu, Jun Li, Li Lin
Summary: This study reveals that hemorrhagic venereal infection or tissue injury can result in the release of hemoglobin, which induces oxidative stress and inflammation in head-kidney macrophages. The oxidative activity of hemoglobin upregulates proinflammatory cytokine expression and induces apoptosis in macrophages.
Article
Immunology
Xiaoxue Yin, Xiaoyu Li, Liangliang Mu, Hao Bai, Yanjian Yang, Nuo Chen, Liting Wu, Shengli Fu, Jun Li, Wantao Ying, Jianmin Ye
Summary: This study reveals the association between site-specific high-mannose modification, IgM antibody affinity, and structural disulfide polymerization. The combination of IgM and mannose receptor amplifies the phagocytic ability of phagocytes, contributing to the immune defense against microbial infection.
JOURNAL OF IMMUNOLOGY
(2022)