Article
Food Science & Technology
Gustavo A. Figueroa Campos, Johannes G. K. T. Kruizenga, Sorel Tchewonpi Sagu, Steffen Schwarz, Thomas Homann, Andreas Taubert, Harshadrai M. Rawel
Summary: This study investigated the changes in the protein fraction of coffee, which is important for cup quality. The results showed a significant increase in modified peptides from wet processed green beans compared to dry ones. This study contributes to a better understanding of the influence of different processing methods on protein quality and its role in coffee cup quality and aroma.
Article
Chemistry, Applied
Chang-Ho Kim, Soo Jin Park, Jeong Seok Yu, Do Yup Lee
Summary: This study aims to characterize the metabolite profiles of farm-grown coffee, focusing on the effects of post-harvest processing, roasting degree, and brewing methods. The results showed that roasting degree was the major discriminant factor, while post-harvest processing and brewing methods also had some influence on the metabolite profiles. Additionally, the study identified novel compounds associated with potential health benefits.
Article
Agronomy
Erika Tatiana Cortes-Macias, Cristina Fuentes Lopez, Piergiorgio Gentile, Joel Giron-Hernandez, Ana Fuentes Lopez
Summary: Different post-harvest methods and roasting intensity were studied for their impact on physicochemical characteristics, antioxidant capacity and bioactive compounds content of Colombia Arabica coffee samples. Results showed strong modifications in parameters like color, antioxidant capacity, and 5-hydroxymethylfurfural content due to roasting. PCA explained 87.4% of sample variability, with antioxidants and hydroxymethylfurfural related to roasting degree.
POSTHARVEST BIOLOGY AND TECHNOLOGY
(2022)
Review
Chemistry, Applied
Leah M. Munyendo, Daniel M. Njoroge, Eddy E. Owaga, Beatrice Mugendi
Summary: The balance between coffee phytochemicals and post-harvest handling is complex, and studies have shown positive health effects arising from regular consumption of coffee. The variation in coffee post-harvest handling techniques affects the content of phytochemicals, resulting in brew with varied sensory and functional properties. Further research on how coffee phytochemicals are affected during post-harvest handling practices would unlock the health benefits of this popular beverage.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2021)
Article
Dentistry, Oral Surgery & Medicine
Seonghye Kim, Hyun-Joo Kim, Yuri Song, Hyun Ah Lee, Suhkmann Kim, Jin Chung
Summary: This study aimed to propose biomarker candidates for periodontitis through untargeted metabolomics analysis. 5 metabolites were identified as biomarkers that distinguished periodontitis patients from healthy controls, showing potential applications in periodontal screening, detection, and monitoring of treatment outcomes.
JOURNAL OF CLINICAL PERIODONTOLOGY
(2021)
Article
Chemistry, Applied
Fareeya Kulapichitr, Chaleeda Borompichaichartkul, Mingchih Fang, Inthawoot Suppavorasatit, Keith R. Cadwallader
Summary: The drying process affects the qualitative indicators of green coffees to varying degrees, with sun drying and heat pump drying resulting in comparable levels of CGAs and antioxidant activities. Specific CGAs, antioxidant activities, and color parameters are correlated among coffees. Heat pump drying at 50 degrees C is a viable alternative and possibly superior to sun drying for preserving certain desirable chemical and physical characteristics of green coffee, according to results from CGAs contents and PCA analysis.
Article
Multidisciplinary Sciences
Rujikorn Treeriya, Phuc N. Ho, Attapol Titapun, Poramate Klanrit, Manida Suksawat, Thanaporn Kulthawatsiri, Suphasarang Sirirattanakul, Watcharin Loilome, Nisana Namwat, Arporn Wangwiwatsin, Nittaya Chamadol, Narong Khuntikeo, Jutarop Phetcharaburanin
Summary: In this study, nuclear magnetic resonance (NMR) metabolomics was used to investigate the fecal metabolic phenotypes of different study groups, including normal bile duct, periductal fibrosis (PDF), and cholangiocarcinoma (CCA) patients. The results revealed distinct fecal metabolic patterns in PDF and CCA patients compared to the normal bile duct group, indicating the involvement of various metabolic pathways in PDF and CCA progression. The study also highlighted the role of gut-microbial host metabolic crosstalk in PDF and/or CCA patients.
Article
Biochemistry & Molecular Biology
Michal Halagarda, Pawel Obrok
Summary: Coffee is a popular beverage known for its sensory properties and psychoactive effects. This study evaluated the impact of different post-harvest coffee fruit treatments on the antioxidant and psychoactive properties of Arabica coffee. The results showed that the processing method did not significantly affect the total phenolic content or antiradical activity of coffee, but differences in water content, pH, and titratable acidity may influence the sensory profile of coffee.
Article
Chemistry, Applied
Flavio Meira Borem, Giselle Figueiredo de Abreu, Antonio Gilberto Ferreira, Maiara da Silva Santos, Thayana da Conceicao Alves, Ana Paula de Carvalho Alves
Summary: NMR was used to identify metabolic changes in green coffee beans stored in different packages. The sensory quality of beverages deteriorated more in paper bags after 6 months of storage, while high barrier bags and vacuum packs maintained quality for 18 months. NMR allowed the differentiation of coffee packed in paper bags from the 3rd month of storage, providing an early identification of changes that would otherwise only be noticed through sensory analysis after six months. Choline and glycerophosphocholine were the main compounds associated with the observed changes in coffee stored in paper bags.
Article
Biochemistry & Molecular Biology
Javier Gallardo-Ignacio, Anislada Santibanez, Octavio Oropeza-Mariano, Ricardo Salazar, Rosa Mariana Montiel-Ruiz, Sandra Cabrera-Hilerio, Manases Gonzales-Cortazar, Francisco Cruz-Sosa, Pilar Nicasio-Torres
Summary: Mexico is known for its specialty and organic coffee, mainly produced by small indigenous community cooperatives. The physical, chemical, and biological characteristics of different roasts of Coffea arabica varieties were analyzed. The study found that the caffeine and melanoidin contents increased with the level of roasting, while the chlorogenic acid content showed a dissimilar effect. Premium and specialty coffees were classified based on their nutritional content and sensory evaluation.
Review
Horticulture
Edy Subroto, Mohamad Djali, Rossi Indiarto, Elazmanawati Lembong, Nur Baiti
Summary: Cocoa beans, which are used to produce chocolate and related products, are greatly influenced by microbial activity during post-harvest handling, especially fermentation, drying, and storage. This review explores the factors affecting each stage, the process mechanisms, and the latest technologies for improving cocoa bean quality. Fermentation plays a crucial role in determining cocoa bean quality as it involves various microorganisms that produce flavor compounds. Drying and storage processes are also important to maintain quality and prevent microbial contaminants. Various technologies, including the use of microbial starters during fermentation and vacuum drying and controlled atmosphere during storage, can enhance cocoa bean quality. However, controlling microbial activity remains a challenge, necessitating continuous development of post-harvest technology.
Article
Food Science & Technology
Ammar Mohammed Ahmed Ali, Sakina A. Yagi, Ahmed A. A. Qahtan, Abdurrahman A. Alatar, Simone Angeloni, Filippo Maggi, Giovanni M. Caprioli, Eslam M. Abdel-Salam, Kouadio Ibrahime Sinan, Gokhan Zengin
Summary: This study evaluated the phytochemical contents and antioxidant and enzyme inhibition activity of six Yemeni green coffee beans varieties. The results showed that all varieties were rich in polyphenols and exhibited significant antioxidant and enzyme inhibition properties. These beans are beneficial to human health and could have significant applications in the food, pharmaceutical, and cosmetics industries.
Article
Forestry
Charlotte E. Norris, Sylvie A. Quideau, Se-Woung Oh, Mathew J. B. Swallow, Barbara E. Kishchuk
Summary: The boreal forest is facing increasing disturbances, particularly wildfires and harvesting, which may impact the forest floor and soil microbial activity. The separate and compound effects of wildfire and harvesting on soil properties were compared in the mixedwood boreal forest of Alberta. Salvage-logging had a greater impact on the recovery of forest floor microbial communities than harvest or wildfire alone.
FOREST ECOLOGY AND MANAGEMENT
(2023)
Review
Food Science & Technology
Mingchun Wen, Mengting Zhu, Zisheng Han, Chi-Tang Ho, Daniel Granato, Liang Zhang
Summary: This review summarizes the recent advances in tea metabolomics research. By using different analytical tools, the metabolite variation of tea leaves and the changes of chemical composition under different processing technologies were studied. The importance of metabolite identification process was also discussed.
COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY
(2023)
Review
Food Science & Technology
Dilek Eltemur, Peter Robatscher, Michael Oberhuber, Matteo Scampicchio, Alberto Ceccon
Summary: Nuclear magnetic resonance (NMR) spectroscopy is a promising technique for the analysis of bovine milk due to its non-destructive nature, minimal sample preparation requirements, and comprehensive approach. This review provides an overview of the applications of NMR techniques in assessing the quality and authenticity of bovine milk, highlighting key studies and emphasizing the versatility and significance of NMR spectroscopy in milk metabolomics research.
Article
Geriatrics & Gerontology
Francesca Di Cesare, Leonardo Tenori, Gaia Meoni, Anna Maria Gori, Rossella Marcucci, Betti Giusti, Raffaele Molino-Lova, Claudio Macchi, Silvia Pancani, Claudio Luchinat, Edoardo Saccenti
Summary: This study investigates the metabolite-lipidic component association networks in the serum of 355 Italian nonagenarians and ultra-nonagenarian, showing different connectivity patterns in lipids related to sex and clinical conditions. The findings suggest that characterizing metabolic structures and their dynamic inter-connections can shed light on the dimorphic pathophysiological mechanisms of aging at the molecular level.
Article
Neurosciences
Gaia Meoni, Leonardo Tenori, Sebastian Schade, Cristina Licari, Chiara Pirazzini, Maria Giulia Bacalini, Paolo Garagnani, Paola Turano, Claudia Trenkwalder, Claudio Franceschi, Brit Mollenhauer, Claudio Luchinat
Summary: Parkinson's disease is the neurological disorder with the highest increase in prevalence. The lack of precise diagnosis at early stages remains a challenge. Metabolomics has provided valuable insights into the molecular basis of PD and potential biomarkers for early detection and treatment efficacy. In this study, NMR was used to analyze serum samples from German PD patients, revealing more pronounced pathological characteristics in male patients and confirming altered levels of acetone and cholesterol. Additionally, stronger oxidative stress markers were detected.
NPJ PARKINSONS DISEASE
(2022)
Article
Biochemical Research Methods
Alessia Vignoli, Leonardo Tenori, Cristina Morsiani, Paola Turano, Miriam Capri, Claudio Luchinat
Summary: Blood derivatives are commonly used in metabolomic clinical studies due to their low invasiveness and rich biological information. However, the choice of blood collection tubes can affect the metabolic content of plasma and serum. In this study, the metabolomic and lipoprotein profiles of blood samples collected using different tubes were compared. Results showed significant alterations in metabolites and correlations among the three blood matrices. The influence of blood collection tubes on lipoprotein profiles was also observed but to a lesser extent. Overcoming the issue of different collection tubes is crucial for scaling metabolomics and lipoprotein analysis in epidemiological studies. A statistical solution based on regression was proposed to reduce the alterations induced by different collection tubes for both metabolomic and lipoprotein profiles.
JOURNAL OF PROTEOME RESEARCH
(2022)
Editorial Material
Chemistry, Multidisciplinary
Alessia Vignoli, Gaia Meoni, Leonardo Tenori
APPLIED SCIENCES-BASEL
(2022)
Article
Chemistry, Multidisciplinary
Linda Cerofolini, Kiefer O. Ramberg, Luis C. Padilla, Pawel Antonik, Enrico Ravera, Claudio Luchinat, Marco Fragai, Peter B. Crowley
Summary: Protein frameworks are emerging biomaterials with potential medical and technological applications. This study introduces a complementary strategy using solid-state NMR analysis to characterize low-crystallinity frameworks, specifically focusing on a microcrystalline protein-macrocycle framework and its rehydrated freeze-dried form.
CHEMICAL COMMUNICATIONS
(2023)
Article
Biochemical Research Methods
Cristina Licari, Leonardo Tenori, Francesca Di Cesare, Claudio Luchinat, Betti Giusti, Ada Kura, Rosina De Cario, Domenico Inzitari, Benedetta Piccardi, Mascia Nesi, Cristina Sarti, Francesco Arba, Vanessa Palumbo, Patrizia Nencini, Rossella Marcucci, Anna Maria Gori, Elena Sticchi
Summary: Metabolic perturbations and inflammatory mediators play a fundamental role in adverse post-acute ischemic stroke outcomes. This study found associations between specific metabolic features and outcomes such as hemorrhagic transformation, non-response to thrombolytic treatment, and functional outcome. These metabolic changes indicate a state of energy failure, oxidative stress, and systemic inflammation.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Agriculture, Dairy & Animal Science
M. Franzoi, G. Niero, G. Meoni, L. Tenori, C. Luchinat, M. Penasa, M. Cassandro, M. De Marchi
Summary: This study evaluates mid-infrared spectroscopy (MIRS) as a rapid method for predicting cow milk metabolites determined through proton nuclear magnetic resonance (1H NMR) spectroscopy. The results show that MIRS can accurately predict certain milk metabolites, but its prediction performance for other metabolites is poor. Further research is needed to explore the practical application of this method in the dairy industry.
JOURNAL OF DAIRY SCIENCE
(2023)
Article
Chemistry, Multidisciplinary
Fabio Carniato, Marco Ricci, Lorenzo Tei, Francesca Garello, Chiara Furlan, Enzo Terreno, Enrico Ravera, Giacomo Parigi, Claudio Luchinat, Mauro Botta
Summary: This article describes nanogels based on a chitosan matrix stabilized by a Mn-t-CDTA bisamide derivative. These nanogels are proposed as an alternative to less stable paramagnetic nanogels obtained by electrostatic interactions. The novel nanogels have improved relaxivity values, high stability, good extravasation and accumulation, and high T-1 contrast in tumors. These properties make them promising MRI probes for in vitro and in vivo preclinical applications.
Article
Oncology
Edda Russo, Leandro Di Gloria, Giulia Nannini, Gaia Meoni, Elena Niccolai, Maria Novella Ringressi, Simone Baldi, Renato Fani, Leonardo Tenori, Antonio Taddei, Matteo Ramazzotti, Amedeo Amedei
Summary: This study comprehensively investigates the microbial spatial variations and their role in CRC progression, identifying potential microbial and metabolic biomarkers and analyzing and comparing metabolites obtained from CRC and AP patients. The results highlight the importance of microbiota communities and oncometabolites in CRC development.
Article
Biochemistry & Molecular Biology
Francesca Di Cesare, Alessia Vignoli, Claudio Luchinat, Leonardo Tenori, Edoardo Saccenti
Summary: This study analyzed the association networks of serum metabolites and found that the metabolite-metabolite association network of colorectal cancer (CRC) patients is distinct from that of patients with polyposis and healthy controls. Energy metabolism-related nodes play a crucial role in the CRC network.
Article
Agriculture, Multidisciplinary
Anton Shiriaev, Stefano Brizzolara, Carlo Sorce, Gaia Meoni, Chiara Vergata, Federico Martinelli, Elie Maza, Anis Djari, Julien Pirrello, Beatrice Pezzarossa, Fernando Malorgio, Pietro Tonutti
Summary: This study investigated the effects of enriching tomatoes with selenium during the last stages of fruit development, particularly ripening. Selenium enrichment had no significant effects on ethylene production or color changes in the ripening fruit but influenced the biochemical composition of ripe tomatoes. Selenium decreased beta-carotene content, increased naringenin and chlorogenic acid accumulation, and decreased coumaric acid level. It also affected the volatile organic compound profile, including specific apocarotenoid compounds. RNA-seq analysis showed that selenium treatment mainly impacted gene expression related to hormonal signaling, secondary metabolism, flavonoid biosynthesis, and glycosaminoglycan degradation.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Agriculture, Multidisciplinary
Anton Shiriaev, Stefano Brizzolara, Carlo Sorce, Gaia Meoni, Chiara Vergata, Federico Martinelli, Elie Maza, Anis Djari, Julien Pirrello, Beatrice Pezzarossa, Fernando Malorgio, Pietro Tonutti
Summary: This study investigated the effects of selenium enrichment on tomato ripening and found that selenium had an impact on metabolic processes and the biochemical composition of ripe tomatoes. The changes in metabolites may be due to the influence of selenium treatment on gene expression.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)