Comprehensive Genome Engineering Toolbox for Microalgae Nannochloropsis oceanica Based on CRISPR-Cas Systems

Synthetic Biology Approaches To Enhance Microalgal Productivity

(2021) Mihris Ibnu Saleem Naduthodi et al.   TRENDS IN BIOTECHNOLOGY

Genome engineering of Nannochloropsis with hundred‐kilobase fragment deletions by Cas9 cleavages

(2021) Qintao Wang et al.   PLANT JOURNAL

Efficient multiplexed gene regulation in Saccharomyces cerevisiae using dCas12a

(2021) Klaudia Ciurkot et al.   NUCLEIC ACIDS RESEARCH

Enhanced lipid productivity in AGP knockout marine microalga Tetraselmis sp. using a DNA-free CRISPR-Cas9 RNP method

(2020) Kwang Suk Chang et al.   BIORESOURCE TECHNOLOGY

Genome editing with the CRISPR‐Cas system: an art, ethics and global regulatory perspective

(2020) Debin Zhang et al.   PLANT BIOTECHNOLOGY JOURNAL

CRISPRi-dCas12a: A dCas12a-Mediated CRISPR Interference for Repression of Multiple Genes and Metabolic Engineering in Cyanobacteria

(2020) Sun Young Choi et al.   ACS Synthetic Biology

Efficient and multiplexable genome editing using Platinum TALENs in oleaginous microalga, Nannochloropsis oceanica NIES‐2145

(2020) Tomokazu Kurita et al.   GENES TO CELLS

Optimization of high-throughput lipid screening of the microalga Nannochloropsis oceanica using BODIPY 505/515

(2020) Christian Südfeld et al.   Algal Research-Biomass Biofuels and Bioproducts

CRISPR–Cas ribonucleoprotein mediated homology-directed repair for efficient targeted genome editing in microalgae Nannochloropsis oceanica IMET1

(2019) Mihris Ibnu Saleem Naduthodi et al.   Biotechnology for Biofuels

Application of the CRISPR/Cas system for genome editing in microalgae

(2019) Yu-Ting Zhang et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Modular one-pot assembly of CRISPR arrays enables library generation and reveals factors influencing crRNA biogenesis

(2019) Chunyu Liao et al.   Nature Communications

Multiplexed genome engineering by Cas12a and CRISPR arrays encoded on single transcripts

(2019) Carlo C. Campa et al.   NATURE METHODS

A high-capacity gene stacking toolkit for the oleaginous microalga, Nannochloropsis oceanica CCMP1779

(2019) Eric Poliner et al.   Algal Research-Biomass Biofuels and Bioproducts

Progress of CRISPR-Cas Based Genome Editing in Photosynthetic Microbes

(2018) Mihris Ibnu Saleem Naduthodi et al.   Biotechnology Journal

An enhanced CRISPR repressor for targeted mammalian gene regulation

(2018) Nan Cher Yeo et al.   NATURE METHODS

Unrestrained markerless trait stacking inNannochloropsis gaditanathrough combined genome editing and marker recycling technologies

(2018) John Verruto et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Nontransgenic Marker-Free Gene Disruption by an Episomal CRISPR System in the Oleaginous Microalga, Nannochloropsis oceanica CCMP1779

(2018) Eric Poliner et al.   ACS Synthetic Biology

Progress of CRISPR-Cas Based Genome Editing in Photosynthetic Microbes

(2018) Mihris Ibnu Saleem Naduthodi et al.   Biotechnology Journal

Optimizing CRISPR/Cas9 for the Diatom Phaeodactylum tricornutum

(2018) Daniel Stukenberg et al.   Frontiers in Plant Science

Lipid production in Nannochloropsis gaditana is doubled by decreasing expression of a single transcriptional regulator

(2017) Imad Ajjawi et al.   NATURE BIOTECHNOLOGY

A toolkit for Nannochloropsis oceanica CCMP1779 enables gene stacking and genetic engineering of the eicosapentaenoic acid pathway for enhanced long-chain polyunsaturated fatty acid production

(2017) Eric Poliner et al.   PLANT BIOTECHNOLOGY JOURNAL

A gene-within-a-gene Cas9/sgRNA hybrid construct enables gene editing and gene replacement strategies in Chlamydomonas reinhardtii

(2017) Wen Zhi Jiang et al.   Algal Research-Biomass Biofuels and Bioproducts

Multiplex gene regulation by CRISPR-ddCpf1

(2017) Xiaochun Zhang et al.   Cell Discovery

Improving heterologous membrane protein production in Escherichia coli by combining transcriptional tuning and codon usage algorithms

(2017) Nico J. Claassens et al.   PLoS One

Combinatorial optimization of CRISPR/Cas9 expression enables precision genome engineering in the methylotrophic yeast Pichia pastoris

(2016) Astrid Weninger et al.   JOURNAL OF BIOTECHNOLOGY

CRISPR interference (CRISPRi) for gene regulation and succinate production in cyanobacterium S. elongatus PCC 7942

(2016) Chun-Hung Huang et al.   Microbial Cell Factories

Multiplex gene editing by CRISPR–Cpf1 using a single crRNA array

(2016) Bernd Zetsche et al.   NATURE BIOTECHNOLOGY

Genome-wide analysis reveals specificities of Cpf1 endonucleases in human cells

(2016) Daesik Kim et al.   NATURE BIOTECHNOLOGY

Editing the epigenome: technologies for programmable transcription and epigenetic modulation

(2016) Pratiksha I Thakore et al.   NATURE METHODS

Metabolic regulation of triacylglycerol accumulation in the green algae: identification of potential targets for engineering to improve oil yield

(2016) Elton C. Goncalves et al.   PLANT BIOTECHNOLOGY JOURNAL

Genome editing of model oleaginous microalgaeNannochloropsisspp. by CRISPR/Cas9

(2016) Qintao Wang et al.   PLANT JOURNAL

DNA-free two-gene knockout in Chlamydomonas reinhardtii via CRISPR-Cas9 ribonucleoproteins

(2016) Kwangryul Baek et al.   Scientific Reports

CRISPR/Cas9-induced knockout and knock-in mutations in Chlamydomonas reinhardtii

(2016) Sung-Eun Shin et al.   Scientific Reports

Water use and its recycling in microalgae cultivation for biofuel application

(2015) Wasif Farooq et al.   BIORESOURCE TECHNOLOGY

Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System

(2015) Bernd Zetsche et al.   CELL

CRISPR-Cas9-Mediated Genome Editing in Leishmania donovani

(2015) Wen-Wei Zhang et al.   mBio

Multiple Gene Repression in Cyanobacteria Using CRISPRi

(2015) Lun Yao et al.   ACS Synthetic Biology

Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation

(2014) Luke A. Gilbert et al.   CELL

Successful Transient Expression of Cas9 and Single Guide RNA Genes in Chlamydomonas reinhardtii

(2014) Wenzhi Jiang et al.   EUKARYOTIC CELL

CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes

(2013) Luke A. Gilbert et al.   CELL

Landfill leachate – a water and nutrient resource for algae-based biofuels

(2013) Scott J. Edmundson et al.   ENVIRONMENTAL TECHNOLOGY

Optical control of mammalian endogenous transcription and epigenetic states

(2013) Silvana Konermann et al.   NATURE

CRISPR interference (CRISPRi) for sequence-specific control of gene expression

(2013) Matthew H Larson et al.   Nature Protocols

Genome engineering using the CRISPR-Cas9 system

(2013) F Ann Ran et al.   Nature Protocols

Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system

(2013) David Bikard et al.   NUCLEIC ACIDS RESEARCH

Demonstration of CRISPR/Cas9/sgRNA-mediated targeted gene modification in Arabidopsis, tobacco, sorghum and rice

(2013) Wenzhi Jiang et al.   NUCLEIC ACIDS RESEARCH

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

Engineered Luciferase Reporter from a Deep Sea Shrimp Utilizing a Novel Imidazopyrazinone Substrate

(2012) Mary P. Hall et al.   ACS Chemical Biology

Environmental Performance of Algal Biofuel Technology Options

(2012) Venkatesh Vasudevan et al.   ENVIRONMENTAL SCIENCE & TECHNOLOGY

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

Genome, Functional Gene Annotation, and Nuclear Transformation of the Heterokont Oleaginous Alga Nannochloropsis oceanica CCMP1779

(2012) Astrid Vieler et al.   PLoS Genetics

High-efficiency homologous recombination in the oil-producing alga Nannochloropsis sp.

(2011) O. Kilian et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The role of biochemical engineering in the production of biofuels from microalgae

(2010) Jorge Alberto Vieira Costa et al.   BIORESOURCE TECHNOLOGY

An Outlook on Microalgal Biofuels

(2010) R. H. Wijffels et al.   SCIENCE

Photosynthetic performance of outdoor Nannochloropsis mass cultures under a wide range of environmental conditions

(2009) A Sukenik et al.   AQUATIC MICROBIAL ECOLOGY

The ecology of algal biodiesel production

(2009) Val H. Smith et al.   TRENDS IN ECOLOGY & EVOLUTION

Microalgae for oil: Strain selection, induction of lipid synthesis and outdoor mass cultivation in a low-cost photobioreactor

(2008) Liliana Rodolfi et al.   BIOTECHNOLOGY AND BIOENGINEERING

Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances

(2008) Qiang Hu et al.   PLANT JOURNAL

Rare Codons Cluster

(2008) Thomas F. Clarke et al.   PLoS One

Biodiesel from microalgae beats bioethanol

(2008) Yusuf Chisti   TRENDS IN BIOTECHNOLOGY