Article
Biochemistry & Molecular Biology
Xinlei Li, Zhaohui Xu, Bidisha Mitra, Minghang Wang, Haitao Guo, Zongdi Feng
Summary: Our study showed that culturing NTCP-reconstituted HepG2 cells in a commercial human iPSC-derived hepatocyte maintenance medium (HMM) significantly increased susceptibility to HBV infection, likely through activation of the NTCP promoter by HMM. However, even with enhanced NTCP expression by HMM, no apparent HBV spread was observed in vitro.
CELL AND BIOSCIENCE
(2021)
Article
Multidisciplinary Sciences
Jinta Asami, Kanako Terakado Kimura, Yoko Fujita-Fujiharu, Hanako Ishida, Zhikuan Zhang, Yayoi Nomura, Kehong Liu, Tomoko Uemura, Yumi Sato, Masatsugu Ono, Masaki Yamamoto, Takeshi Noda, Hideki Shigematsu, David Drew, So Iwata, Toshiyuki Shimizu, Norimichi Nomura, Umeharu Ohto
Summary: Chronic infection with hepatitis B virus (HBV) requires interaction between the large glycoproteins of the virus envelope (LHBs) and the host entry receptor sodium taurocholate co-transporting polypeptide (NTCP). This study reveals the structures of NTCP and identifies a possible transport route for substrate. Furthermore, the study suggests a binding mode between LHBs and NTCP, which explains naturally occurring HBV-insusceptible mutations in NTCP.
Article
Gastroenterology & Hepatology
Kira A. A. T. Lowjaga, Michael Kirstgen, Simon F. Muller, Nora Goldmann, Felix Lehmann, Dieter Glebe, Joachim Geyer
Summary: The study demonstrates that TLC can rapidly accumulate in NTCP-expressing cells, leading to a long-lasting trans-inhibition of NTCP's transporter and receptor function. This suggests a potential novel target site for pharmacological long-acting HBV/HDV entry inhibitors.
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
(2021)
Article
Gastroenterology & Hepatology
Takayoshi Shirasaki, Kazuhisa Murai, Atsuya Ishida, Kazuyuki Kuroki, Kazunori Kawaguchi, Ying Wang, Souma Yamanaka, Rio Yasukawa, Narumi Kawasaki, Ying-Yi Li, Tetsuro Shimakami, Ariunaa Sumiyadorj, Kouki Nio, Saiho Sugimoto, Noriaki Orita, Hideo Takayama, Hikari Okada, Phuong Doan Thi Bich, Sadahiro Iwabuchi, Shinichi Hashimoto, Mayuko Ide, Noriko Tabata, Satoru Ito, Kouji Matsushima, Hiroshi Yanagawa, Taro Yamashita, Shuichi Kaneko, Masao Honda
Summary: The study found that endothelial lipase (LIPG) participates in HBV infection by increasing HBV attachment to the cell membrane. LIPG achieves this through two possible mechanisms: increasing HBV attachment to HSPGs or promoting HSPG-dependent or independent HBV attachment to NTCP through its lipase activity.
HEPATOLOGY COMMUNICATIONS
(2023)
Article
Gastroenterology & Hepatology
Takayoshi Shirasaki, Kazuhisa Murai, Atsuya Ishida, Kazuyuki Kuroki, Kazunori Kawaguchi, Ying Wang, Souma Yamanaka, Rio Yasukawa, Narumi Kawasaki, Ying-Yi Li, Tetsuro Shimakami, Ariunaa Sumiyadorj, Kouki Nio, Saiho Sugimoto, Noriaki Orita, Hideo Takayama, Hikari Okada, Phuong Doan Thi Bich, Sadahiro Iwabuchi, Shinichi Hashimoto, Mayuko Ide, Noriko Tabata, Satoru Ito, Kouji Matsushima, Hiroshi Yanagawa, Taro Yamashita, Shuichi Kaneko, Masao Honda
Summary: In this study, it was found that endothelial lipase (LIPG) participates in HBV infection by upregulating HBV attachment to the cell membrane through two possible mechanisms: increasing HBV attachment to heparan sulfate proteoglycans (HSPGs) or facilitating HSPG-dependent or HSPG-independent HBV attachment to sodium taurocholate cotransporting peptide (NTCP) by its lipase activity.
HEPATOLOGY COMMUNICATIONS
(2023)
Review
Biochemistry & Molecular Biology
Yan Li, Jun Zhou, Tianliang Li
Summary: Hepatitis B virus (HBV) is a globally prevalent human DNA virus causing chronic liver infection, with the transmembrane protein NTCP identified as the receptor for cellular entry of HBV. Various cell culture and animal models have been established for studying HBV biology and developing treatments for infection. Understanding the NTCP biology could contribute to the design of new therapeutic drugs for preventing liver cell infection.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Pharmacology & Pharmacy
Hsing Huang, Hsiu-Chen Huang, Wei-Chung Chiou, Lie-Chwen Lin, Jui-Chieh Chen, Hui-Kang Liu, Yu-Heng Lai, Cheng Huang
Summary: Hepatitis B virus (HBV) infection causes severe liver diseases, but current therapies are unable to eradicate the virus completely. The newly identified inhibitor EP targets the entry step of HBV infection and shows potential for fighting against HBV infection by interfering with the NTCP-LHBsAg interaction.
ANTIVIRAL RESEARCH
(2021)
Article
Pharmacology & Pharmacy
Anna Basile, Carla Zannella, Margot De Marco, Giuseppina Sanna, Gianluigi Franci, Massimiliano Galdiero, Aldo Manzin, Vincenzo De Laurenzi, Massimiliano Chetta, Alessandra Rosati, Maria Caterina Turco, Liberato Marzullo
Summary: Researchers have developed a specific antibody against IFITM2 that can interfere with the invasion of SARS-CoV-2 and reduce its damage to host cells. This antibody also reduces the damage caused by other viruses such as HSV and RSV, suggesting that IFITM2 may have a similar mechanism of invasion to other viruses. These results demonstrate the specific role of IFITM2 in viral entry and its potential as a cell target for antiviral therapies.
ANTIVIRAL RESEARCH
(2023)
Article
Virology
Kento Fukano, Mizuki Oshima, Senko Tsukuda, Hideki Aizaki, Mio Ohki, Sam-Yong Park, Takaji Wakita, Kousho Wakae, Koichi Watashi, Masamichi Muramatsu
Summary: NTCP oligomerization plays a crucial role in HBV internalization, and troglitazone inhibits HBV infection by interfering with NTCP oligomerization. Phenylalanine 274 of NTCP is identified as essential for oligomerization. NTCP oligomerization is initiated downstream of the NTCP-EGFR interaction and triggers HBV internalization. This study provides valuable insights into the mechanism of HBV entry and suggests that targeting NTCP oligomerization could be a potential strategy for drug development.
JOURNAL OF VIROLOGY
(2021)
Article
Virology
Michael Kirstgen, Kira Alessandra Alicia Theresa Lowjaga, Simon Franz Mueller, Nora Goldmann, Felix Lehmann, Dieter Glebe, Karl-Heinz Baringhaus, Joachim Geyer
Summary: This study identified virus-selective inhibitors of NTCP for HBV/HDV through screening different compounds, providing a novel approach for the development of cell-entry inhibitors.
Review
Immunology
Shuling Wu, Wei Yi, Yuanjiao Gao, Wen Deng, Xiaoyue Bi, Yanjie Lin, Liu Yang, Yao Lu, Ruyu Liu, Min Chang, Ge Shen, Leiping Hu, Lu Zhang, Minghui Li, Yao Xie
Summary: Functional cure is considered achieved when chronic hepatitis B patients achieve HBsAg seroclearance after treatment. Clearing the virus is challenging due to the difficulty in clearing both cccDNA and integrated dslDNA. High levels of HBsAg in the serum lead to immune system failure, making it unable to produce an effective antiviral response. Only less than 1% of chronic hepatitis B patients are cured annually with antiviral treatment. Coinfection with other viruses such as HIV, HCV, and HDV makes it even more difficult to cure. However, it has been found that patients with coinfection have a higher probability of achieving HBsAg seroclearance, especially those with HBV/HIV coinfection with up to 36% 5-year seroclerance rate. This interesting phenomenon is related to the functional reconstruction of the immune system after antiretroviral therapy (ART).
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Keiji Ueda, Yadarat Suwanmanee
Summary: The precise mechanism of HBV entry into cells is still largely unknown. However, researchers have identified ATP5B as an essential factor for HBV entry, which is believed to bind with myristoylated preS1 2-47. Knockdown of ATP5B in NTCP-expressing HepG2 cells reduced HBV infectivity, indicating that ATP5B is involved in the HBV entry process.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, General & Internal
Jun Li, Zhao Wu, Gui-Qiang Wang, Hong Zhao
Summary: The study revealed correlations between HBcrAg and antiviral efficacy, virological, and histological variables in chronic hepatitis B patients, with a decreased HBcrAg predicting HBeAg loss after antiviral therapy.
CHINESE MEDICAL JOURNAL
(2021)
Review
Gastroenterology & Hepatology
Norah A. Terrault, Miriam T. Levy, Ka Wang Cheung, Gonzague Jourdain
Summary: The management of viral hepatitis in pregnancy requires special consideration due to the unique characteristics of each hepatitis virus and their impact on maternal and infant outcomes. Preventing mother-to-child transmission is crucial in reducing the global burden of chronic viral hepatitis.
NATURE REVIEWS GASTROENTEROLOGY & HEPATOLOGY
(2021)
Article
Virology
Muhammad Atif Zahoor, Adrian Kuipery, Alexander I. Mosa, Adam J. Gehring, Jordan J. Feld
Summary: In this study, subclones of HepG2-NTCP-A3 with increased permissiveness to HBV were isolated. One subclone showed more than four-fold higher HBV infection compared to the parental clone. The subclone also maintained greater permissiveness under PEG-free conditions, indicating its potential for developing a PEG-free HBV infection model.
Article
Multidisciplinary Sciences
Khanit Sa-ngiamsuntorn, Piyanoot Thongsri, Yongyut Pewkliang, Suparerk Borwornpinyo, Adisak Wongkajornsilp
Summary: The study introduces an effective hepatocyte model for screening anti-HBV drugs targeting both pre-viral entry and post-viral entry lifecycle stages, providing important insights for finding new approaches to treating hepatitis B.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2022)
Article
Chemistry, Organic
Sitthivut Charoensutthivarakul, Duangporn Lohawittayanan, Phongthon Kanjanasirirat, Kedchin Jearawuttanakul, Sawinee Seemakhan, Suparerk Borwornpinyo, Matthew Phanchana
Summary: Quinazolinedione, with its broad spectrum of biological activities including antimalarial, anticancer, and anti-inflammatory properties, was synthesized using cost-effective and greener alternatives. The synthesized compound, TCMDC-125133, showed promising antimalarial activity and was evaluated for its antiproliferative activity.
Article
Pharmacology & Pharmacy
Kunlakanya Jitobaom, Chompunuch Boonarkart, Suwimon Manopwisedjaroen, Nuntaya Punyadee, Suparerk Borwornpinyo, Arunee Thitithanyanont, Panisadee Avirutnan, Prasert Auewarakul
Summary: The present study demonstrated the benefit of drug combinations on anti-SARS-CoV-2 activity. Niclosamide and ivermectin showed the best synergistic profile and should be further tested in clinical trials.
BMC PHARMACOLOGY & TOXICOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Kanit Bhukhai, Guillemette Fouquet, Yutthana Rittavee, Nopmullee Tanhuad, Chaiyaporn Lakmuang, Suparerk Borwornpinyo, Usanarat Anurathapan, Apichart Suksamrarn, Pawinee Piyachaturawat, Arthit Chairoungdua, Olivier Hermine, Suradej Hongeng
Summary: ASPP 049, a phytoestrogen diarylheptanoid isolated from Curcuma comosa, promotes erythropoiesis through activation of the Epo-Epo receptor (EpoR) complex, inducing EpoR-mediated erythropoiesis pathways and improving anemia symptoms.
Article
Medicine, General & Internal
Wipawee Booranapong, Panida Kosrirukvongs, Sunisa Duangsa-ard, Kanda Kasetsinsombat, Khanit Sa-ngiamsuntorn, Adisak Wongkajornsilp
Summary: This study evaluated the efficacy and safety of using cultivated oral mucosal cells as autologous grafts for corneal replacement in patients with limbal stem cell deficiency. The results showed favorable outcomes, with patients who suffered chemical burns benefiting more clinically compared to those with SJS.
JOURNAL OF MEDICAL CASE REPORTS
(2022)
Article
Oncology
Stefan Schreier, Prapaphan Budchart, Suparerk Borwornpinyo, Wichit Arpornwirat, Panuwat Lertsithichai, Prakasit Chirappapha, Wannapong Triampo
Summary: This study proposes the detection of CD44-related systemic inflammation as a method for assessing residual cancer in early-stage breast cancer. By analyzing the morphological characteristics of circulating CD44+/CD45- rare cells, the detection of residual cancer can be improved, providing an objective measure of disease burden.
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
(2023)
Article
Oncology
Atthapol Srimongkol, Natanan Laosillapacharoen, Duangporn Saengwimol, Vijender Chaitankar, Duangnate Rojanaporn, Thanastha Thanomchard, Suparerk Borwornpinyo, Suradej Hongeng, Rossukon Kaewkhaw
Summary: This study aimed to identify drugs with improved antitumor activity and more favorable retinal toxicity profiles through screening of potentially repurposable FDA-approved drugs in patient-derived tumor organoids. The results showed that sunitinib was highly cytotoxic against RB organoids and had better efficacy and lower toxicity compared to melphalan and topotecan.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2023)
Article
Multidisciplinary Sciences
Thanida Chanpong, Watee Seesuay, Wararat Chiangjong, Piamsiri Jiramornimit, Sarinthip Preedagasamzin, Korakot Atjanasuppat, Bunyada Jittorntrum, Somsak Prasongtanakij, Supannikar Tawinwung, Sulada Pukiat, Chonticha Saisawang, Suparerk Borwornpinyo, Khanit Sa-ngiamsuntorn, Wanpen Chaichumpa, Suradej Hongeng, Usanarat Anurathapan
Summary: CD30, a membrane epitope on cancer cells, can be a potential target for lymphoma treatment. By using phage technology, a novel mCD30 monoclonal antibody HuscFv #A4 was discovered and proved to specifically eradicate CD30-expressing cancers. Chimeric antigen receptor-modified T lymphocytes using HuscFv #A4 showed significant cytotoxicity against CD30-expressing cell line.
Article
Allergy
Kittipong Maneechotesuwan, Phunsup Wongsurakiat, Jirawat Assawabhumi, Kanda Kasetsinsombat, Adisak Wongkajornsilp
Summary: It was found that TAK1 expression was increased in asthmatic patients with fixed airway obstruction, independent of the number of eosinophils. This suggests that TAK1 may play a significant role in asthmatic patients with fixed airway obstruction.
JOURNAL OF ASTHMA AND ALLERGY
(2023)
Article
Biochemical Research Methods
Kanokorn Wechakorn, Chariwat Pitsanuwong, Phongthon Kanjanasirirat, Yongyut Pewkliang, Suparerk Borwornpinyo, Palangpon Kongsaeree
Summary: A highly selective rhodamine-triazole fluorescent probe was synthesized using Cu(I)-catalyzed click reaction, which can detect Cu2+ in physiological conditions. The probe is sensitive to Cu2+ and exhibits fluorescence emission in the presence of Cu2+. The detection limit was determined to be 21 nM (1.3 ppb) using fluorescence titration. It can also be applied for fluorescence imaging of Cu2+ in cells.
JOURNAL OF FLUORESCENCE
(2023)
Article
Plant Sciences
Prapairat Seephonkai, Chatthai Kaewtong, Phurpa Wangchuk, Kedchin Jearawuttanakul, Phongthon Kanjanasirirat, Suparerk Borwornpinyo, Tachin Khulmanee, Rapatbhorn Patrapuvich
Summary: In this study, six compounds were isolated from the stem bark of Clausena excavata, including five pyranocoumarins and one coumarin. Compounds 3 and 4 exhibited potent antiplasmodial activities against the multidrug-resistant Plasmodium falciparum strain K1.
Article
Virology
Jitra Limthongkul, Kornkamon Akkarasereenon, Tanpitcha Yodweerapong, Poramate Songthammawat, Pirut Tong-Ngam, Alisa Tubsuwan, Nawapol Kunkaew, Phongthon Kanjanasirirat, Tanawadee Khumpanied, Warawuth Wannalo, Sukathida Ubol, Suparerk Borwornpinyo, Poonsakdi Ploypradith, Marisa Ponpuak
Summary: This study discovered a novel autophagy inhibitor, Ka-003, which can inhibit DENV replication. Ka-003 has low cytotoxicity and shows potential for dengue treatment.
Article
Multidisciplinary Sciences
Janpen Tunganuntarat, Phongthon Kanjanasirirat, Tanawadee Khumpanied, Salisa Benjaskulluecha, Benjawan Wongprom, Tanapat Palaga, Tegar Adriansyah Putra Siregar, Suparerk Borwornpinyo, Angkana Chaiprasert, Prasit Palittapongarnpim, Marisa Ponpuak
Summary: Starvation-induced autophagy enhances lysosomal delivery to mycobacterial phagosomes, limiting the growth of Mycobacterium tuberculosis. However, the Beijing genotype of M. tuberculosis can evade autophagic elimination. This study reveals that the autophagy-resistant Beijing strain evades autophagic control by upregulating the expression of Kxd1 and Plekhm2, inhibiting lysosomal delivery to the phagosomes.
SCIENTIFIC REPORTS
(2023)
Article
Virology
Kunlakanya Jitobaom, Chompunuch Boonarkart, Suwimon Manopwisedjaroen, Nuntaya Punyadee, Suparerk Borwornpinyo, Arunee Thitithanyanont, Panisadee Avirutnan, Prasert Auewarakul
Summary: The combination of favipiravir and ivermectin has synergistic effects against SARS-CoV-2, and the combination of favipiravir with niclosamide also shows antiviral activity. However, the efficacy of favipiravir may vary in different cell types.