期刊
NATURE
卷 606, 期 7916, 页码 1021-+出版社
NATURE PORTFOLIO
DOI: 10.1038/s41586-022-04845-4
关键词
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资金
- Japanese Ministry of Education, Culture, Sports, Science and Technology [19H03164, 19H00976, 18K05334, 19H00923]
- Knut and Alice Wallenberg foundation
- Platform Project for Supporting Drug Discovery and Life Science Research (Basis for Supporting Innovative Drug Discovery and Life Science Research (BINDS)) from Japan Agency for Medical Research and Development (AMED) [JP19am0101115, 1570, 1846, 1848, JP21am0101079]
- AMED Research Program on Emerging and Re-emerging Infectious Disease [20fk0108270h0001]
- JST Core Research for Evolutional Science and Technology [JPMJCR20HA]
- JSPS Core-to-Core Program A
- Joint Usage/Research Center Program of Institute for Frontier Life and Medical Sciences, Kyoto University
- Joint Research Project of the Institute of Medical Science, the University of Tokyo
- Grants-in-Aid for Scientific Research [19H00923, 19H00976, 19H03164, 18K05334] Funding Source: KAKEN
Chronic infection with hepatitis B virus (HBV) requires interaction between the large glycoproteins of the virus envelope (LHBs) and the host entry receptor sodium taurocholate co-transporting polypeptide (NTCP). This study reveals the structures of NTCP and identifies a possible transport route for substrate. Furthermore, the study suggests a binding mode between LHBs and NTCP, which explains naturally occurring HBV-insusceptible mutations in NTCP.
Chronic infection with hepatitis B virus (HBV) affects more than 290 million people worldwide, is a major cause of cirrhosis and hepatocellular carcinoma, and results in an estimated 820,000 deaths annually(1,2). For HBV infection to be established, a molecular interaction is required between the large glycoproteins ofthe virus envelope (known as LHBs) and the host entry receptor sodium taurocholate co-transporting polypeptide (NTCP), a sodium-dependent bile acid transporter from the blood to hepatocytes(3). However, the molecular basis for the virus-transporter interaction is poorly understood. Here we report the cryo-electron microscopy structures of human, bovine and rat NTCPs in the apo state, which reveal the presence of a tunnel acrossthe membrane and a possible transport route for the substrate. Moreover, the cryo-electron microscopy structure of human NTCP in the presence of the myristoylated preS1 domain of LHBs, together with mutation and transport assays, suggest a binding mode in which preS1 and the substrate compete for the extracellular opening ofthe tunnel in NTCP. Our preS1 domain interaction analysis enables a mechanistic interpretation of naturally occurring HBV-insusceptible mutations in human NTCP. Together, our findings provide a structural framework for HBV recognition and a mechanistic understanding of sodium-dependent bile acid translocation by mammalian NTCPs.
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